Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
권호 표지

Identification of Excision of Ac Transposable Element in P.nigra x maximowiczii Using Agrobacterium-mediated Transformation

  • Ahn, In-Suk (Department of Forestry, North Carolina University, USA) ;
  • Park, Young-Goo (Department of Forest, Kyungpook National University) ;
  • Shin, Dong-Ill (Department of Plant genetic engineering, Catholic University Daegu) ;
  • Sul, Ill-Whan (Department of Biotechnology, Daegu University of Foreign Studies)
  • Published : 2003.03.01
Download PDF
⟨ Previous Next ⟩

Abstract

The Ac (activator) which is one of the well-characterized transposable elements from maize was examined for its transposition possibility to the heterologous plant (P.nigra x maximowiczii) genome via Agrobacterium tumefacience (LBA4404) mediated transformation system. A number of transgenic plants were successfully recovered after 30 weeks by amount reduction from 50 to 15 g/$m\ell$ kanamycin for in vitro selection to minimize phytotoxic effects and to increase callus growth and regeneration efficiency. Among transgenic plants, 62 out of 106 transgenic poplars (58.5%) showed abnormal phenotypes such as severe serrated leaves and light leaf coloration. Indigo staining with X-gluc proved indirectly the restoration of Gus enzyme function and the presence of Ac in poplar genome by PCR. Southern analysis indicated the transposition and existence of Ac element in poplar genomes. In this research, an Agrobacterium-mediated transformation system in poplar species was developed and identified that Ac derived from maize can be excised and trans posed into other poplar genomes.

Keywords

References

  1. Plant J v.3 Mobility of the maize transposable element En/Spm in Arabidopsis thaliana. Cardon GH;Grey M;Saedler H;Gierl A https://doi.org/10.1111/j.1365-313X.1993.00773.x
  2. Transgenic plants v.1 Gene tagging in transgenic plant. In Kung SD, Wu R (eds) Coomber SA;Feldmann KA
  3. Ann Rev Genet v.20 Molecular genetics of transposable lements in plants. Doring HP;Stralinger P https://doi.org/10.1146/annurev.ge.20.120186.001135
  4. Maize transposable elements in Mobile DNA (Douglas E. Berg and Martha M. Howe) Fedoroff NN
  5. Plant Cell Tiss Org Cult v.20 Effect of thidiazuron, light influence, rates and kanamycin on in vitro shoot organogenesis from excised Rubus cotyledons and leaves. Fiola JA;Hassan MA;Swartz HK;Bors RH;Cc Nicols R
  6. Plant Mol Biol v.33 Excision of the maize transposable element Ac in periclinal chimeric leaves of 35S-Ac-rolC transgenic aspen-Populus. Flaudung M;Ahuja MR https://doi.org/10.1023/A:1005788706864
  7. Mol Gen Genet v.217 The maize En-1/Spm element transposes in potato Frey M;Tavantzis SM;Saedler H https://doi.org/10.1007/BF00330958
  8. Plant Mol Biol v.19 Plant-transposble elements and gene tagging. Gierl A;Saedler H https://doi.org/10.1007/BF00015605
  9. Biotechnology of perennial fruit crops. Grape. In Hammerschlag FA, Litz RE (eds) Gray DJ;Beredith CP
  10. Molecular Biology of Rice Ac/Dsas tools for rice molecular biology. In K. Shimamoto (ed) Izawa T;Simamoto K
  11. Plant Tiss. Cult. Manual. v.B8 Regeneration and transformation of apple Malus pumilla Mill. In Lindsey K (ed) James DJ;Dandekar AM
  12. Plant Mol Biol Rep v.5 Assayin gchimeric genes in plant: The GUS gene fusion system. Jefferson RA https://doi.org/10.1007/BF02667740
  13. Plant Bol Biol v.16 Transpositionof the maize activator element in transgenic rice plant. Murray EE;Rochelea, u T;Eberle M;Stock C;Sekar V;Adang M https://doi.org/10.1007/BF00016075
  14. Physiol Plant v.15 A revised medium for rapid growth and bioasays with tobacco cultures. Murashige R;Skoog F https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  15. Plant J v.4 Instability of the L6 gene for rust resistance in flax is correlated with the presence a linked Ac element. Lawrence G;Finnegan J;Ellis J https://doi.org/10.1046/j.1365-313X.1993.04040659.x
  16. Mol Gen Gent v.245 Modification of the 5' untranslated leader region of the maize Activator element leads to increased activity in Arabidopsis. Lawson EJR;Scofield SR;Sjodin C;Jones JDG;Dean C
  17. In vitro culture of higher plants. Pierik RLM
  18. Rec Adv Phytochem v.2 Plant tissue culture as a technique fof the phytochemist. Staba EJ