• Asian Pacific Journal of Cancer Prevention
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• 제13권11호
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• pp.5391-5397
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• 2012

Background: Neuroblastoma (NB), like most human cancers, is characterized by genomic instability, manifested at the chromosomal level as allelic gain, loss or rearrangement. Genetics methods, as well as conventional and molecular cytogenetics may provide valuable clues for the identification of target loci and successful search for major genes in neuroblastoma. We aimed to investigate AURKA and MYCN gene rearrangements and the chromosomal aberrations (CAs) to determine the prognosis of neuroblastoma. Methods: We performed cytogenetic analysis by G-banding in 25 cases [11 girls (44%) and 14 boys (66%)] and in 25 controls. Fluorescence in situ hybridization (FISH) with AURKA and MYCN gene probes was also used on interphase nuclei to screen for alterations. Results: Some 18.4% of patient cells exhibited CAs., with a significant difference between patient and control groups in the frequencies (P<0.0001). Some 72% of the cells had structural aberrations, and only 28% had numerical chnages in patients. Structural aberrations consisted of deletions, translocations, breaks and fragility in various chromosomes, 84% and 52% of the patients having deletions and translocations, respectively. Among these expressed CAs, there was a higher frequency at 1q21, 1q32, 2q21, 2q31, 2p24, 4q31, 9q11, 9q22, 13q14, 14q11.2, 14q24, and 15q22 in patients. 32% of the patients had chromosome breaks, most frequently in chromosomes 1, 2, 3, 4, 5, 8, 9, 11, 12, 19 and X. The number of cells with breaks and the genomic damage frequencies were higher in patients (p<0.001). Aneuploidies in chromosomes X, 22, 3, 17 and 18 were most frequently observed. Numerical chromosome abnormalities were distinctive in 10.7% of sex chromosomes. Fragile sites were observed in 16% of our patients. Conclusion: Our data confirmed that there is a close correlation between amplification of the two genes, amplification of MYCN possibly contributing significantly to the oncogenic properties of AURKA. The high frequencies of chromosomal aberrations and amplifications of AURKA and MYCN genes indicate prognostic value in children with neuroblastomas and may point to contributing factors in their development.

• 한국안광학회지
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• 제19권4호
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• pp.487-492
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• 2014

목적: 대물부의 구성이 더블렛+메니스커스 렌즈를 가지는 3X 스코프 개발에 관한 것이다. 방법: 더블렛(doublet) + 단일렌즈(singlet)의 구성을 가지는 대물부를 초기조건으로 하여 대물부의 유한광선 수차와 스코프 전체 광학계의 유한광선 수차를 최소화하도록 스코프 광학계를 최적화하여 새로운 형태의 3X 스코프를 개발하였다. 결과: 대물부의 구성을 더블렛+단일렌즈로 두고 유한광선 수차를 최소화하도록 스코프 광학계를 최적화를 하였을 때 우리는 단일렌즈는 더블렛 방향으로 오목한 메니스커스 형태의 렌즈가 되며, 더블렛과 메니스커스 렌즈 사이의 거리가 멀수록 유한광선 수차는 더욱 최소화됨을 확인할 수 있었다. 결론: 본 연구에서 우리는 3X 스코프의 대물부를 더블렛+메니스커스 렌즈 방식의 3매의 렌즈를 채택함으로서 기존의 스코프보다 유한광선 수차를 약 1/14로 줄일 수 있는 새로운 형태의 3X 스코프를 개발할 수 있었다. 이러한 수차량의 감소는 기존 스코프 보다 유효 구경을 크게 할 수 있으며 광학계의 길이를 짧게 할 수 있는 수단이 됨을 확인할 수 있었다.

• 한국유전체학회:학술대회논문집
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• 한국유전체학회 2004년도 The 13th Korea Genome Conference
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• pp.46.1-46.1
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• 2004

• 한국광학회:학술대회논문집
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• 한국광학회 2002년도 제13회 정기총회 및 2002년도 동계학술발표회
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• pp.208-209
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• 2002

• 한국광학회:학술대회논문집
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• 한국광학회 2002년도 제13회 정기총회 및 2002년도 동계학술발표회
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• pp.104-105
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• 2002

• 한국환경성돌연변이발암원학회지
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• 제18권2호
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• pp.89-92
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• 1998

The results of chromosome aberration test in mammalian cells in culture (Chinese hamster lung fibroblast cells) showed no induction of structural and numerical aberrations by YH1226, a cephalosporin antibiotic regardless of metabolic activation, while positive control group (mitomycin C and benzo(a)pyrene) showed structural chromosome aberrations of 25% and 10%, respectively. The in vivo induction of micronuclei was measured in polychromatic erythrocytes in bone marrow of male ddY mouse given YH1226 at 500, 250, 125 mg/kg by i.p. once. After 24 hours, animals were sacrificed and evaluated for the incidence of micronucleated polychromatic erythrocytes in whole erythrocytes. Although a positive response for induction of micronuclei in animals treated with mitomycin C demonstrated the sensitivity of the test system for detection of a chemical clastogen, YH1226 did not induce microunclei in bone marrow of ddY male mice.

• Toxicological Research
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• 제34권4호
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• pp.311-324
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• 2018

Arsenic is one of the most toxic environmental toxicants. More than 150 million people worldwide are exposed to arsenic through ground water contamination. It is an exclusive human carcinogen. Although the hallmarks of arsenic toxicity are skin lesions and skin cancers, arsenic can also induce cancers in the lung, liver, kidney, urinary bladder, and other internal organs. Arsenic is a non-mutagenic compound but can induce significant cytogenetic damage as measured by chromosomal aberrations, sister chromatid exchanges, and micronuclei formation in human systems. These genotoxic end points are extensively used to predict genotoxic potentials of different environmental chemicals, drugs, pesticides, and insecticides. These cytogenetic end points are also used for evaluating cancer risk. Here, by critically reviewing and analyzing the existing literature, we conclude that inorganic arsenic is a genotoxic carcinogen.

• 한국식품위생안전성학회지
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• 제1권1호
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• pp.13-21
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• 1986

Two short-term bioassays were employed to asses the mutagenic and clastogenic activities in browning reaction of pentose-creatine, pentose-glycine and pentose-creatine-glycine browning reaction model system. Methylene chloride extract of rhamnose-creatine-glycine browning reaction exhibited the strongest mutagenicity toward Salmonella typhimurium TA98 with S-9. Methylene chloride extract of pentose-creatine and pentose-glycine browning reaction solutions was also tested for mutagenicity, with positive responses. Methylene chloride extract of pentose-creatine-glycine browning reaction solutions induced significant increase in chromosome aberrations in the treated Chinese hamster ovary(CHO) cells. Each of pentose-creatine and pentose-glycine browning reaction solutions induced a relatively low frequency of chromosome aberrations in the treated cells.

• 환경생물
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• 제21권4호
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• pp.384-391
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• 2003

The induction of chromosome aberrations in Hordeum vulgare germs after irradiation is studied for the dose range of 10 to 1,000 mGy. The relationship between the frequency of aberrant cells and the absorbed dose is shown to be non -linear and has a dose-independent plateau within the range of 56-467 mGy where the level of cytogenetic damage is statistically significantly distinguished from the spontaneous level. The comparison of the goodness of the experimental data fitting with mathematical models of different complexities, using the most common quantitative criteria, demonstrates the benefit of the piecewise linear model over the linear and polynomial ones in approximating the cytogenetical disturbance frequency. The results of our study support the conclusion about indirect mechanism of chromosome aberrations induced by low doses or dose rates mutagenesis.

• 한국광학회지
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• 제9권3호
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• pp.142-145
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• 1998

가우스 함수로 진폭변조한 우인한 가우스 동에 작은 Seidel 제 1차 수차가 있을때의 회절진폭 분포를 해석적으로 구하였다. 즉, 무수차 가우스 동의 회절진폭 분포와 수차에 의한 회절효과의 함으로 구하였다. 각 수차의 표현은 파면수차함수의 환산좌표를 공간각주파구로 대치하여, 수차에 의한 회절상을 되풀이 공식(recurrence formula)으로 구하였다.