• Toxicological Research
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• v.30 no.3
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• pp.211-220
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• 2014

Resveratrol has received considerable attention as a polyphenol with various biological effects such as anti-inflammatory, anti-oxidant, anti-mutagenic, anti-carcinogenic, and cardioprotective properties. As part of the overall safety assessment of HS-1793, a novel resveratrol analogue free from the restriction of metabolic instability and the high dose requirement of resveratrol, we assessed genotoxicity in three in vitro assays: a bacterial mutation assay, a comet assay, and a chromosomal aberration assay. In the bacterial reverse mutation assay, HS-1793 did not increase revertant colony numbers in S. typhimurium strains (TA98, TA100, TA1535 and TA1537) or an E. coli strain (WP2 uvrA) regardless of metabolic activation. HS-1793 showed no evidence of genotoxic activity such as DNA damage on L5178Y $Tk^{+/-}$ mouse lymphoma cells with or without the S9 mix in the in vitro comet assay. No statistically significant differences in the incidence of chromosomal aberrations following HS-1793 treatment was observed on Chinese hamster lung cells exposed with or without the S9 mix. These results provide additional evidence that HS-1793 is non-genotoxic at the dose tested in three standard tests and further supports the generally recognized as safe determination of HS-1793 during early drug development.

• Laboratory Medicine Online
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• v.7 no.2
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• pp.83-87
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• 2017

Pseudohypoparathyroidism (PHP) is a rare disorder caused by genetic and epigenetic aberrations in the GNAS complex locus resulting in impaired expression of stimulatory G protein ($Gs{\alpha}$). PHP type Ib (PHP-Ib) is characterized by hypocalcemia and hyperphosphatemia due to renal resistance to the parathyroid hormone, and is distinguished from PHP-Ia by the absence of osteodystrophic features. An 11-yr-old boy presented with poor oral intake and cramping lower limb pain after physical activity. Laboratory studies revealed hypocalcemia, hyperphosphatemia, and increased parathyroid hormone levels. The GNAS complex locus was evaluated using the methylation-specific multiplex ligation-dependent probe amplification (MS-MLPA) assay. Gain of methylation in the NESP55 domain and loss of methylation in the antisense (AS) transcript, XL, and A/B domains in the maternal allele were observed. Consequently, we present a case of PHP-Ib diagnosed using MS-MLPA.

• Korean Journal of Optics and Photonics
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• v.14 no.3
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• pp.236-243
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• 2003

We discussed the design of lens module schematic eyes equivalent to finite model eyes, which are used to model the human eye based on spherical aberration and Stiles-Crowford effect. The optical system for head mounted display (HMD) is designed and evaluated using lens module schematic eyes. In addition to a compact HMD system, an optical system with high Performance is required. To satisfy these requirements, we used diffractive optical elements and aspheric surfaces so that the color and mono-chromatic aberrations were corrected. The optical system for HMD is composed of 0.47 inch micro-display of SVGA grade with 480,000 pixels, a plastic hybrid lens for the virtual image, and the lens module schematic eyes. The designed optical system fulfills the current specifications of HMD: such as, EFL of 31.25 mm, FOV of 24H$\times$18V$\times$30D degrees, and overall length of 59.1 mm. As a result, we could design an optical system useful for HMD; the system is expected to be comfortable while the user wears it.

• Journal of the Optical Society of Korea
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• v.17 no.4
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• pp.336-343
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• 2013

Recently various types of driver assistance systems have been used for automobiles. In 2008, the U.S Congress passed a law which required that most cars be equipped with devices to warn objects behind the vehicle. Because of that, market of rear view cameras is expected to rise dramatically. Therefore many suppliers try to provide a wide angle camera for car makers. But a high distortion is caused by the wide angle might result in lower image quality. In order to improve the image quality, normally we use an algorithm to correct a distortion. Though we can improve the distorted image by correction algorithm, we must pay more cost to use it. In this paper, we propose a new optical system reducing a distortion in contrast to a conventional lens without cost. In other words, we can see only an area of interest. That is similar to reducing a field of view. Using a new optical system, we can get a less distorted image. In order to view an area of interest, we introduce an off axis optical system having refractive surfaces and reflective surfaces. In this paper, we describe the results of design and, evaluation of an off axis wide angle compact imaging system. In comparison to conventional wide angle lens, we can get the improvement of MTF, distortion, and lateral color aberrations. And we also can reduce a total cost because we don't need the outer apparatus or bracket to mount on the car.

• Korean Journal of Optics and Photonics
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• v.28 no.5
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• pp.187-193
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• 2017

The purpose of this study was to investigate some implications for optics education and improvement of the Examination for Appointing Secondary School Physics Teachers (EASSPT), by analyzing the problems in EASSPT. For these purpose, we analyzed 38 optics problems of the EASSPT from the 16 school years of 2002 to 2017. The results of the analysis are as follows: First, optics problems were presented in 10.8% of the physics subject area, which was least frequently. Second, many problems have addressed specific areas such as Snell's law, image of a single lens, or interference. Few problems were found in many other evaluation areas, including optical instruments and aberrations. Third, compared to other areas, optics problems were presented as relatively easy questions, and the problems were presented in more laboratory contexts than daily-life contexts. In addition, we have discussed implications for EASSPT and in-service physics-teacher training.

• Korean Journal of Optics and Photonics
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• v.28 no.5
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• pp.241-249
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• 2017

This paper presents the design and evaluation of the optical system for an uncooled thermal-imaging camera. The operating wavelength range of this system is from $7.7{\mu}m$ to $12.8{\mu}m$. Through optimization, we have obtained a LWIR (Long Wave Infrared) optical system with a focal length of 5.44 mm, which consists of four aspheric surfaces and two diffractive surfaces. The f-number of the optical system is F/1.2, and its field of view is $90^{\circ}{\times}67.5^{\circ}$. The hybrid lens was used to balance the higher-order aberrations, and its diffraction properties were evaluated by scalar diffraction theory. We calculated the polychromatic integrated diffraction efficiency, and the MTF drop generated by background noise. We have evaluated the thermal compensation of a LWIR fixed optical system, which is optically passively athermalized to maintain MTF performance in the focal depth. In conclusion, these design results are useful for an uncooled thermal-imaging camera.

• Journal of Genetic Medicine
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• v.16 no.1
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• pp.10-14
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• 2019

Purpose: The aim of this study was to investigate the clinicopathological features of premature ovarian insufficiency (POI) associated with chromosomal abnormalities. Materials and Methods: This was a retrospective study of POI patients with chromosomal abnormalities diagnosed between January 2009 and December 2017. The definition of POI is based on hypergonadotropinism of 40 or greater in follicle stimulating hormone (FSH) measurements at age 40 years or less. FSH was measured twice at least 4 weeks apart. Karyotyping using peripheral blood for chromosomal testing was conducted in all patients diagnosed with POI. We analyzed the clinical characteristics and genetic causes of patients who were diagnosed with POI. Results: Forty patients were diagnosed with POI including 9 (22.5%) with identified chromosomal abnormalities. The mean age at diagnosis was $23.1{\pm}7.8years$ (ranging between 14 and 39). Three patients did not experience menarche. The presenting complaints were short stature in one case, one case of amenorrhea with ambiguous external genitals, one case of infertility, and six related to menstruation such as oligomenorrhea or irregular rhythm. Turner syndrome was diagnosed in four cases, Xq deletion in one case, trisomy X in two cases, and 46,XY disorder of sexual development in two other patients. Conclusion: Patients diagnosed with POI carrying the same type of chromosomal abnormality manifest different phenotypes. The management protocol also needs to be changed depending on the diagnosis. A karyotype is indicated for accurate diagnosis and proper management of POI in patients, with or without stigmata of chromosomal abnormalities.

• Clinical and Experimental Reproductive Medicine
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• v.46 no.1
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• pp.22-29
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• 2019

Objective: As paternal age increases, the quality of sperm decreases due to increased DNA fragmentation and aneuploidy. Higher levels of structural chromosomal aberrations in the gametes ultimately decrease both the morphologic quality of embryos and the pregnancy rate. In this study, we investigated whether paternal age affected the euploidy rate. Methods: This study was performed using the medical records of patients who underwent in vitro fertilization (IVF) procedures with preimplantation genetic screening (PGS) from January 2016 to August 2017 at a single center. Based on their morphological grade, embryos were categorized as good- or poor-quality blastocysts. The effects of paternal age were elucidated by adjusting for maternal age. Results: Among the 571 total blastocysts, 219 euploid blastocysts were analyzed by PGS (38.4%). When the study population was divided into four groups according to both maternal and paternal age, significant differences were only noted between groups that differed by maternal age (group 1 vs. 3, p= 0.031; group 2 vs. 4, p= 0.027). Further analysis revealed no significant differences in the euploidy rate among the groups according to the morphological grade of the embryos. Conclusion: Paternal age did not have a significant impact on euploidy rates when PGS was performed. An additional study with a larger sample size is needed to clarify the effects of advanced paternal age on IVF outcomes.

• Korean Journal of Optics and Photonics
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• v.33 no.6
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• pp.275-286
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• 2022

As a first step in the optical design process, we derive a recursive numerical calculation method that can give a solution to the Gaussian equation that the paraxial rays satisfy. Given the refractive power, the angle of incidence to the first principal plane of the lens, the angle of exit to the second principal plane of the lens, and the distance between the principal planes, the radii of curvature of the front and back surfaces of a lens can be obtained by applying the recursive numerical calculation method proposed in this paper according to the thickness of the lens. If a module consists of two or more lenses, the thickness and radius of curvature of each lens can be similarly determined after selecting the distance between the principal planes of the lens under the condition of the design specification while increasing the number of lenses one by one.

• Clinical and Experimental Reproductive Medicine
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• v.49 no.3
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• pp.210-214
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• 2022

Objective: Platelet-rich plasma (PRP) therapy has received a considerable attention as an adjunct to fertility treatments, especially in women with very low ovarian reserve and premature ovarian insufficiency. Although recent studies have demonstrated that PRP led to improvements in folliculogenesis and biomarkers of ovarian reserve, the effect of intraovarian PRP administration on embryo genetics has not been studied. Methods: We report a pilot study of patients who had preimplantation genetic testing for aneuploidy (PGT-A) before and then within 3 months following PRP administration. Twelve infertile women with at least one prior failed in vitro fertilization (IVF) cycle underwent ovarian stimulation (cycle 1) with a gentle stimulation protocol and PGT-A performed at the blastocyst stage. Following cycle 1, autologous intraovarian PRP administration was performed. Within 3 months following PRP administration, the patients underwent cycle 2 and produced blastocysts for PGT-A. The percentage of euploid embryos between both cycles was compared. Results: The mean age of all participants was 40.08±1.46 years, and their mean body mass index was 26.18±1.18 kg/m². The number of good-quality embryos formed at the blastocyst stage was similar between cycle 1 and cycle 2 (3.08±0.88 vs. 2.17±0.49, respectively; p=0.11). Among all patients in cycle 1, 3 of 37 embryos were euploid (8.11%) while in cycle 2, 11 out of 28 embryos were euploid (39.28%, p=0.002). Three clinical pregnancies were noted among this patient group. Conclusion: This novel study is the first to present an improvement in the embryo euploidy rate following intraovarian PRP application in infertile women with prior failed IVF cycles. The growth factors present in PRP may exhibit a local paracrine effect that could improve meiotic aberrations in human oocytes and thus improve euploidy rates. Whether PRP improves live birth rates and lowers miscarriage rates remains to be determined in large trials.