• Fisheries and Aquatic Sciences
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• v.17 no.2
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• pp.249-253
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• 2014

An 8-week feeding experiment was conducted to determine the influence of dietary distillers dried grain (DDG) on the growth and body composition of juvenile abalone Haliotis discus hannai. Five diets were formulated to contain 0% (DDG0), 15% (DDG15), 30% (DDG30), 45% (DDG45), and 60% (DDG60) DDG, and three replicate groups of abalone (average body weight: $3.6{\pm}0.21$ g) were fed one of the experimental diets at a feeding rate of 5% body weight per day once daily (17:00 h) for 8 weeks. Survival, shell length, and shell width of juvenile abalone were not affected by dietary DDG levels (P > 0.05). Weight gains of juvenile abalone fed DDG15 and DDG30 diets were not different compared to DDG0, but abalone fed DDG45 and DDG60 diets gained less weight than those fed DDG0 (P < 0.05). Soft body weight/body weight ratio of juvenile abalone fed the DDG60 diet was lower than that of those fed the DDG0 diet (P < 0.05), but proximate composition of the soft body was not affected by dietary DDG levels (P > 0.05). The results of this experiment suggest that DDG is a good replacement for wheat flour and soybean meal, and can be used up to 30% in the diet to maintain the growth performance of the juvenile abalone.

• Journal of Aquaculture
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• v.11 no.1
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• pp.31-38
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• 1998

A 20-week growth trial was conducted in flow-through aquarium system to investigate the effects of supplemental macroalgae and spirulina in the diets on growth and body cmposition in juvenile abalone (Haliotis discus hannai). Four replicate groups of the abalone averaging 65mg were fed one of ten isonitrogenous (34%) and isolipidic (7.5%) diets containing 8.1% Ulva, 7.5% Undaria, 11% Laminara, 11% Sargassum, spirulina (5, 10 and 15%), or dried Undaria powder (10 and 20%). In addition, these formulated diets were compared with natural food(dried Undaria). Survival rate of abalone were not significantly affected by the different dietary macoralgae sources, spirulina or dried Undaria powder levels (P>0.05). Weight gain and soft body weight of abalone fed the diet containing Sargassum was significantly higher (P<0.05) than those of abalone fed the diets containing Laminaria, 20% dried Undaria powder and natural food. Survival rate, weight gain, soft body weight and shell length of abalone fed natural food were lowest (P<0.05) among all diet. Moisture, protein and lipid contents of soft body were not influenced by experimental diets except natural food. Lipid content of abalones fed natural food was significantly lower than those of abalone fed other diets (P<0.05). These data indicate that abalone can more efficiently utilize Sargassum than Ulva, Undaria, Laminaria or spirulina.

• Fisheries and Aquatic Sciences
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• v.18 no.2
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• pp.159-164
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• 2015

A feeding experiment was conducted to determine the effect of dietary distillers dried grain (DDG) on the growth and body composition of juvenile abalone, Haliotis discus hannai. Three diets were formulated to contain 0% (DDG0), 30% DDG (DDG1) replacing wheat flour and 30% DDG (DDG2) replacing fish meal and wheat flour. A commercial feed (CF) was also compared with experimental diets. Three replicate groups of abalone averaging $0.6{\pm}0.01g$ were fed one of the four diets for 14 weeks. The highest survival rate was observed in the abalone fed the DDG2 diet, which was higher than that of abalone fed the DDG0 diet. Weight gain, shell length, shell width and soft body weight of the abalone were not affected by dietary DDG and CF diet (P > 0.05). Proximate and amino acid composition of the soft whole body were not affected by dietary DDG and CF diet (P > 0.05). The result of this experiment suggests that DDG is a good ingredient to replace fish meal and wheat flour in the diet and could be used up to 30% in the diet without negative effects on the growth performance of juvenile abalone Haliotis discus hannai.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.50 no.6
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• pp.738-744
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• 2017

Effect of dietary inclusion of microalgae, diatom-dominant, oil extracts (MOE) on growth, body composition and shell color of juvenile abalone Haliotis discus was investigated. One thousand four hundred and seventy juvenile abalone were distributed into 21 plastic rectangular containers. Seven experimental diets were prepared: MOE0, MOE0.01, MOE0.05, MOE0.1, MOE0.5, MOE1 and MOE2 diets containing MOE at the concentrations of 0, 0.01, 0.05, 0.1, 0.5, 1 and 2% at the expense of mixture of squid liver and soybean oils, respectively. The experimental diets were fed to abalone in triplicate once a day with a little leftover for 16 weeks. Weight gain and specific growth rate of abalone fed the MOE1 and MOE2 diets were higher than those of abalone fed the all other diets. The shell length and soft body weight of abalone fed the MOE2 diet were longer and heavier than those of abalone fed the all other diets. Crude protein and ash content of the soft body of abalone were affected by dietary inclusion of MOE. The shell color of abalone fed the all experimental diets was different from that of wild abalone. In conclusion, dietary inclusion of MOE improved growth of abalone, but did not shell color of abalone.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.44 no.5
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• pp.560-564
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• 2011

Two feeding experiments were conducted to investigate the effects of dietary inclusion of genetically modified (GM) soybean and corn on the growth performance, feed utilization and body composition of juvenile abalone Haliotis discus hannai. Four isonitrogenous (31% crude protein) and isolipidic (6% crude lipid) diets (designated as nGM-soya, GM-soya, nGM-corn and GM-corn) were formulated to contain 20% non-GM (nGM) and GM soya and corn. Fifty juvenile abalone (initial body weight, 2.0 g) were distributed in each 50 L tank in a flow-through system. Each experimental diet was fed to duplicate groups of abalone to satiation once a day for 10 weeks. No effects of GM feedstuffs on survival were observed. Dietary inclusion of GM feedstuffs did not affect either growth performance or feed utilization of abalone. Body composition was not altered by the inclusion of GM feedstuffs. These results indicate that dietary inclusion of GM soybean and corn could have no effect on the growth performance and body composition of juvenile abalone. Further studies to investigate the effects of GM feedstuffs on transgenic fragment residues in ambient environments and in animals are necessary for the safe use of such ingredients in aquaculture.

• Proceedings of the Korean Aquaculture Society Conference
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• 2003.10a
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• pp.73-73
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• 2003

This study was carried out to evaluate the utilization of fermented skipjack tuna viscera (FSTV) in the diet for juvenile abalone Haliotis discus hannai. Lactobacillus bulgaricus was used for fermentation of skipjack tuna viscera. Eight isonitrogenous (about 30% crude protein) diets were formulated to include different levels (0%, 10%, 20% and 30%) of FSTV as a replacer of either dietary fish meal or soybean meal. Three replicate groups of abalone were fed the experimental diets containing different levels of FSTV for 7 weeks. The inclusion of FSTV up to 30% in fish meal-based diet had no significant effect on survival, body weight, shell growth, and proximate composition of abalone (P>0.05). Weight gain of abalone fed the diet substituting 10% FSTV for soybean meal was not significantly different to that of abalone fed the control diet, however this value decreased in abalone fed the 20% and 30% FSTV (P<0.05).The contents of crude protein and lipid of soft body in abalone fed soybean meal-based diets were significantly affected by dietary FSTV level (P<0.05). The results of this study indicate that FSTV can be used as a partial substitute protein source for fish meal or soybean meal in the formulated diet for juvenile abalone.

• Journal of Aquaculture
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• v.12 no.4
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• pp.317-322
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• 1999

A growth trial was conducted to investigate the utilization of wheat germ meal as a protein source of formulated diet for juvenile abalone (Haliotis discus hannai). Four replicate groups of the abalone average weighing 150mg were fed one of four isonitrogenous (33%) and isolipidic (6%) diets containing 0%, 10%, 20%, or 30% wheat germ meal for 18 weeks. In addition, these formulated diets were compared with commercial diet. Survival rate, weight gain, soft body weight , and shell growth of abalone fed diets containing 0%, 10%, 20%, or 30% wheat germ meal were not different (P>0.05) from those of abalonn fed the control diet and commercial diet. There were no significant differences (P>0.05) in soft body composition of moisture, protein and lipid. It si concluded that wheat germ meal were be used as a partial protein source of formulated diet for juvenile abalone.

• Journal of Aquaculture
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• v.11 no.4
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• pp.505-512
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• 1998

This study was conducted to investigate the effects of three different formulated diets and macroalgae(Undaria) on growth and body comoposition among juvenile Haliotis discus, H. sieboldii and H. discus hannail. Three replicate groups among the three species abalones average weighing 0.31g were fed one of one experimental diet, two commercial diets and Undaria for 9 weeks. The three formulated diets produced significantly (P<0.05) greated body and shell growth than Undaria within same abalone species. Survival rates (97~99%) of H. discus and H. sieboldii were higher than that (74~77%) of H. discus hannai. Whereas different diets had no significantly (P>0.05) distincitive effect on survival within each three different abalone species. Moisture of abalone soft body in final stage reduced compared to those of abalone in initial stage in H. discus and H. discus hannai. Regardless of diet, abalone soft body revealed significantly (P<0.05) increased protein and decreased ash at the end of feeding trial in all three abalone species. This study indicate that all three different formulated diets used in this experiment could be a practical diet in three abalone species.

• Food Science and Preservation
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• v.20 no.4
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• pp.441-450
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• 2013

In this study, the general compositions, amino acids, fatty acids and collagen content of three abalones, which were harvested in Wando, Heuksando and Jeju Island in Korea, were analyzed and compared. The abalone body and viscera were separated from each other and the physicochemical properties were investigated. The moisture and protein contents of the viscera were lower than that of the body; while the lipid, carbohydrate, and mineral contents were higher. The general compositions and mineral contents also presented fluctuation among the three abalone groups. The amino acid content via hydrolysis in the abalone viscera ranged from 93.16 to 127.02 mg/g, which was lower than the amino acid content of the abalone body of approximately 145 mg/g. However, the free amino acids level in the abalone viscera ranged from 16.81 to 20.10 mg/g, which was higher than that of the abalone body level of approximately 7.90 to 10.59 mg/g. The fatty acid analysis revealed that while the saturated fatty acid percentages in the abalone body were higher than in the abalone viscera, the percentages of the monoenoic and polyenoic fatty acids of the body were significantly lower. The body of the abalone harvested in Wando contained the highest level of collagen ($2.26{\pm}0.46mg/g$), followed by the abalone harvested in Jeju Island and Heuksando. The abalone viscera contained much lower collagen, which ranged from $0.37{\pm}0.15mg/g$ to $0.20{\pm}0.03mg/g$. The results of this study will provide useful information for the future research of abalone.

• Journal of Aquaculture
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• v.13 no.1
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• pp.55-62
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• 2000

A 10-week feeding trial was conducted to investigate the effects of several yeasts with or without chemical treatment (protoplasted) in formulated diets on growth and body composition of juvenile abalone(Haliotis discus hanai). There replicate groups of the abalone average weighing 210 mg were fed one of eight isonitrogenous (30%) and isolipidic (4.4%) diets containing 3% Kluyveromyces fragilis protoplasted K. fragilis Candida utilis protoplasted C. utilis, Saccharomyces cerevisiae protoplasted S. cerevisiae or brewer's yeast. In addition these formulated diets were compared with commercial diet. Survival rate and proximate analysis of soft whole body of abalone were not significantly affected by the different dietary yeasts and commercial diet (P>0.05) Body weight gain and soft body weight control diet and diets containing S. cerevisiae or brewer's yeast (P<0.05) Shell length of abalone fed yeast and commercial diet (P<0.05) Th results suggest that protoplasted K. fragilis as an additive in this formulated diet can improve weight gain of abalone.