• The Journal of Korean Physical Therapy
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• 제35권1호
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• pp.31-35
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• 2023

Purpose: This study sought to identify whether fascial therapy using myofascial release (MFR) and Fascial Distortion Model (FDM) techniques affected not only the area where treatment was being given but also remote areas connected to the treatment area by fascial continuity through comparison of the pain pressure threshold (PPT). Methods: The subjects were 16 healthy normal adults in their 20s and 30s who were divided into the MFR and FDM groups before the experiment. The PPT was measured at 4 different points on the body of the subjects. C7, T7, L5, and gastrocnemius along the superficial back line (SBL) before and after the intervention. Results: Only the FDM group subjects showed a significant increase in the PPT at T7 after the intervention. (p<0.05). In addition, the FDM group demonstrated significantly increased PPT at L5 compared to the MFR group. However, neither the FDM nor the MFR group showed a meaningful change in the PPT at the remote area in the lower leg. Conclusion: These findings showed that FDM can affect PPT more and has a positive effect on the pain threshold compared to MFR. However, neither FDM nor MFR showed any effect on the PPT in a remote area.

• 한국수산과학회지
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• 제34권6호
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• pp.605-610
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• 2001

이 연구에서는 해산어의 종묘 생산 과정에서 rotifer의 다음 단계 먹이생물로 이용되고 있는 Artemia의 대체 먹이생물로서 Diaphanosoma celebensis의 배양 환경 중 염분 농도가 미치는 영향을 검토하였다. 염분 농도 0 ppt에서 45ppt 사이에서 5 ppt 간격으로 염분 조정된 10단계의 조건하에서 D. celebensis의 증식 밀도, 안정배양 가능정도 (RPGI: Relative Population Growth Index) 그리고 먹이섭 이력 (RCN; Remaining Cell Numbers)을 비교 검토하였다. 그 결과, 염분 농도 0ppt에서 45ppt까지 10단계의 염분 배양 환경하에서 가장 높은 증식 밀도, 안정 배양도 (RPGI) 그리고 양호한 섭이력 (RCN)이 관찰된 실험구는 염분 농도 20ppt였으며, $15\~35ppt$의 염분 배양 환경하에서도 비교적 안정적인 배양 결과가 관찰되었고 20ppt를 중심으로 저염분 또는 고염분 배양 조건으로 갈수록 증식, RPGI 그리고 RCN은 저하되는 경향이 뚜렷하였다. 그리고 염분 농도 0ppt에서는 실험 개시 직후 전 개체가 사망하였다. 이 연구 결과 D. celebensis는 해산어의 종묘 생산 과정에서 Artemia의 대체 먹이생물로 광범위한 염분 농도 범위하에서 비교적 손쉽게 배양 가능할 것으로 판단되었다.

• 한국항공우주학회지
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• 제30권3호
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• pp.46-56
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• 2002

본 논문에서는 플라즈마 펄스 추력기(PPT)를 사용한 우주 비행체의 자세 제어 가용성을 연구하였다. 전형적인 기체 추진 시스템에 비해 PPT는 높은 임펄스 특성을 갖고 있어 궤도나 자세 제어에 필요한 추진제의 질량을 적게 소모한다. PPT는 상대적으로 긴 작동 시간과 장착의 간편성 때문에 장기 작동을 요구하는 임무에 많이 적용되리라 기대된다. 소형 위성 시스템의 자세 제어를 위한 PPT의 적용 가능성을 실제 임무를 통하여 연구하였다. 고전적인 비례 미분 제어기와 퍼지 제어기를 적용해 보았고 점 더 유연한 임무 성능을 위해 연료 절감형 퍼지 제어기를 제안하였다.

• Fisheries and Aquatic Sciences
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• 제8권2호
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• pp.70-75
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• 2005

We investigated the effects of salinity and three food species of microalgae on the growth of three types of the rotifer Brachionus plicatilis, with the aim of improving mass culture of rotifers in hatcheries. Three types (large, small, and ultra-small) of the rotifer were cultured at 16 ppt and 32 ppt salinity with the green algae Chlorella ellipsoidea, Nannochloris oculata, or Tetraselmis tetrathele. The maximum density and specific growth rate were compared for each rotifer type. Ultra-small rotifers grew significantly faster at 16 ppt salinity than at 32 ppt, and C. ellipsoidea and T. tetrathele promoted significantly higher growth than did N. oculata. However, small rotifers grew significantly better at 32 ppt salinity than at 16 ppt, and small rotifers fed on N. oculata achieved the highest density at 1,185 individuals/ml. Large rotifers grew faster at 16 ppt salinity than at 32 ppt, with a diet of T. tetrathele resulting in the fastest growth. Each type of rotifer thrived under different regimens of microalgae and salinity.

• Applied Microscopy
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• 제43권3호
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• pp.103-109
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• 2013

ICI 182,780 (ICI) is known as an estrogen receptor antagonist, whereas propyl pyrazole triol (PPT) is an estrogen receptor agonist. In this study, ICI or ICI added with PPT was injected into adult male mice. Body and reproductive organ weights were reduced in the ICI added with PPT group compared to the control group. Further, the ICI and ICI added with PPT groups both showed increases in luminal areas of the seminiferous tubules of the testis, whereas cell heights of efferent ductules and the initial segment of the epididymis were reduced. Sperm count in the caudal epididymis was reduced in the ICI and ICI added with PPT groups. These results show that reproductive tissues were more deeply affected in the ICI added with PPT group. We also demonstrated that treatment with ICI resulted in histological changes in the testis, efferent ductule, and epididymis. Further, alternate treatment with ICI and PPT induced abnormalities in reproductive organs. These results indicate that a high concentration of PPT together with ICI may cause histological abnormalities instead of histological restoration in reproductive organs.

• Journal of Ginseng Research
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• 제40권1호
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• pp.47-54
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• 2016

Background: The roots of Panax ginseng contain noble tetracyclic triterpenoid saponins derived from dammarenediol-II. Dammarene-type ginsenosides are classified into the protopanaxadiol (PPD) and protopanaxatriol (PPT) groups based on their triterpene aglycone structures. Two cytochrome P450 (CYP) genes (CYP716A47 and CYP716A53v2) are critical for the production of PPD and PPT aglycones, respectively. CYP716A53v2 is a protopanaxadiol 6-hydroxylase that catalyzes PPT production from PPD in P. ginseng. Methods: We constructed transgenic P. ginseng lines overexpressing or silencing (via RNA interference) the CYP716A53v2 gene and analyzed changes in their ginsenoside profiles. Result: Overexpression of CYP716A53v2 led to increased accumulation of CYP716A53v2 mRNA in all transgenic roots compared to nontransgenic roots. Conversely, silencing of CYP716A53v2 mRNA in RNAi transgenic roots resulted in reduced CYP716A53v2 transcription. HPLC analysis revealed that transgenic roots overexpressing CYP716A53v2 contained higher levels of PPT-group ginsenosides ($Rg_1$, Re, and Rf) but lower levels of PPD-group ginsenosides (Rb1, Rc, $Rb_2$, and Rd). By contrast, RNAi transgenic roots contained lower levels of PPT-group compounds and higher levels of PPD-group compounds. Conclusion: The production of PPD- and PPT-group ginsenosides can be altered by changing the expression of CYP716A53v2 in transgenic P. ginseng. The biological activities of PPD-group ginsenosides are known to differ from those of the PPT group. Thus, increasing or decreasing the levels of PPT-group ginsenosides in transgenic P. ginseng may yield new medicinal uses for transgenic P. ginseng.

• 한국육종학회지
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• 제42권4호
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• pp.351-356
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• 2010

Brachypodium distachyon is rapidly emerged in biological study and has been currently used as a model system for genetics and functional studies for crop improvement and biofuel production. Phosphinothricin (PPT) has been widely used as a selectable agent, which raises ammonium content and induces toxicity in non-transformed plant cells. However PPT selection is not much effective on Brachypodium callus consequently reducing transformation efficiency. In order to identify the efficient conditions of PPT selection, calli obtained from mature seeds of Brachypodium (PI 254867) were cultured on the callus inducing medium (CIM) or regeneration medium (ReM) containing serial dilutions of the PPT (0, 2, 5, 10, and 15 mg/l) in dark or light condition. Callus growth and ammonium content of each treatment were measured 2 weeks after the treatment. Although callus growth and ammonium content did not show much difference in CIM, slow callus growth and increased ammonium accumulation were found in ReM. No significant difference of ammonium accumulation in response to PPT was found between dark and light conditions. In order to identify major factors affecting increased ammonium accumulation, callus was cultured on the media in combined with phytohormones (2,4-D or kinetin) and carbon sources (sucrose or maltose) containing with PPT (5 mg/l). The highest ammonium content in callus was found in the kinetin and maltose media.

• 한국수산과학회지
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• 제26권6호
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• pp.511-518
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• 1993

해산어류의 종묘생산시 초기 동물먹이생물로 널리 이용되는 미소형, 소형, 대형 윤충(Brachionus plicatilis)의 환경요인에 따른 수명과 번식력을 조사하였다. 전반적으로 온도는 $25{\sim}27^{\circ}C$가 $20{\sim}22^{\circ}C$ 보다 번식력과 수명이 높았으며 미소형과 소형윤충은 이러한 현상이 더욱 뚜렷하였다. 염분은 미소형과 대형 윤충은 32ppt보다 16ppt에서 번식력이 더욱 높았던 반면 소형 윤충은 32ppt에서 더욱 높았다. 먹이생물은 미소형과 대형 윤충은 Tetraselmis tetrathele 소형 윤충은 Chlorella ellipsoidea가 더 좋은 먹이 효율을 보였다. 각 윤충의 먹이생물, 수온, 염분에 따른 최고의 번식력은 미소형 윤충은 Tetraselmis tetrathele, $25{\sim}27^{\circ}C$, 16ppt, 소형 윤충은 Chlorella ellipsoidea, $25{\sim}27^{\circ}C$, 32ppt ,대형 윤충은 Chlorella ellipsoidea, $25{\sim}27^{\circ}C$, 16ppt에서 나타났다.

• Journal of Ginseng Research
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• 제43권2호
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• pp.261-271
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• 2019

Background: Protopanaxatriol (PPT) is an aglycone of ginsenosides, which has high medicinal values. Production of PPT from natural ginseng plants requires artificial deglycosylation procedures of ginsenosides via enzymatic or physicochemical treatments. Metabolic engineering could be an efficient technology for production of ginsenoside sapogenin. For PPT biosynthesis in Panax ginseng, damarenediol-II synthase (PgDDS) and two cytochrome P450 enzymes (CYP716A47 and CYP716A53v2) are essentially required. Methods: Transgenic tobacco co-overexpressing P. ginseng PgDDS, CYP716A47, and CYP716A53v2 was constructed via Agrobacterium-mediated transformation. Results: Expression of the three introduced genes in transgenic tobacco lines was confirmed by Reverse transcription-polymerase chain reaction (RT-PCR). Analysis of liquid chromatography showed three new peaks, dammarenediol-II (DD), protopanaxadiol (PPD), and PPT, in leaves of transgenic tobacco. Transgenic tobacco (line 6) contained $2.8{\mu}g/g$ dry weight (DW), $7.3{\mu}g/g$ DW, and $11.6{\mu}g/g$ DW of PPT, PPD, and DD in leaves, respectively. Production of PPT was achieved via cell suspension culture and was highly affected by auxin treatment. The content of PPT in cell suspension was increased 37.25-fold compared with that of leaves of the transgenic tobacco. Transgenic tobacco was not able to set seeds because of microspore degeneration in anthers. Transmission electron microscopy analysis revealed that cells of phloem tissue situated in the center of the anther showed an abnormally condensed nuclei and degenerated mitochondria. Conclusion: We successfully achieved the production of PPT in transgenic tobacco. The possible factors deriving male sterility in transgenic tobacco are discussed.

• 융합경영연구
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• 제10권5호
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• pp.15-24
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• 2022

Purpose: The purpose of this study is to find a way to improve and overcome the psychological treatment limited to the negative factors dealt with in psychology from a positive psychological point of view. To this end, this study aims to verify whether a positive psychotherapy program can improve happiness, resilience, and post-traumatic growth along with improvement of psychological symptoms such as depression. Research design, data and methodology: To this end, in this study, mean difference analysis was conducted using t-test on 10 participants in the 16th PPT program and 14 in the control group. Also, after setting the main variables, we tried to confirm the effectiveness through simple regression analysis and multiple regression analysis of the causal relationship model. Results: As a result of the independent sample t-test and the paired sample t-test, it was confirmed that the group participating in the PPT program had higher flourish, happiness, resilience, post-traumatic growth, and lower depression. In addition, as a result of regression analysis, it was confirmed that post-traumatic growth had a positive effect, and that depression was a life-threatening factor. Conclusions: Since the PPT program has a positive effect on the participants with relatively negative psychological symptoms, it is necessary to expand it. In addition, it is necessary to introduce various preventive programs such as PPT as well as traditional psychological treatment for negative symptoms such as depression.