• Steel and Composite Structures
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• v.45 no.1
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• pp.1-21
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• 2022

In the structural analysis of steel frames, joints are generally considered as rigid or hinged considering their moment transfer ability. However, the first studies conducted with the beginning of the 20th century showed that the joints do not actually fit these two definitions. In reality, a joint behaves between these two extreme points and is called semi-rigid. Including the actual state of the joint in the structural analysis provides significant economic advantages, so the subject is an intense field of study today. However, it does not find enough application area in practice. For this reason, a large-scale literature published from the first studies on the subject to the present has been examined within the scope of the study. Three important points have been identified in order to examine a joint realistically; modelling the load-displacement relationship, performing the structural analysis and how to design. Joint modelling methods were grouped under 7 main headings as analytical, empirical, mechanical, numerical, informational, hybrid and experimental. In addition to the moment-rotation, other important external load effects like axial force, shear and torsion were considered. Various evaluations were made to expand the practical application area of semi-rigid connections by examining analysis methods and design approaches. Dynamic behaviour was also included in the study, and besides column-beam connections, other important connection types such as beam-beam, column-beam-cross, base connection were also examined in this paper.

• Journal of Electrochemical Science and Technology
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• v.15 no.2
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• pp.276-290
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• 2024

A study of the transition from transitory state to steady state in DSSCs based on natural dyes is presented; cochineal was used as dye and Li⁺, Na⁺, and K⁺ were the ions added to the electrolyte. The photocurrent profiles were obtained as a function of time. Several DSSCs were prepared with different cations and their role and the transitory-to-steady transition was determined. A novel hybrid charge carrier source model based on the Heaviside function H(t) and the Lambert-Beer law, was developed and applied to analysis of the transient response of the output photocurrent. Additionally, the maximum effective light absorption coefficient α and the electronic extraction rate κ for each ion were determined: ${\alpha}_{Li^+,Na^+,K^+}\,=\,(0.486,\,0.00085,\,0.1126)\,cm^{-1}$, and also the electronic extraction rate ${\kappa}^{Li^+,Na^+,K^+}_{ext.}\,=\,(1410,\,19.07,\,19.69)\,cm\,s^{-1}$. The impedance model using Fick's second law was developed for carrier recombination to characterize the photocurrent.

• Korean Journal of Microbiology
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• v.35 no.4
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• pp.315-321
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• 1999

This study has been performed to deveope a yeast strain having high ${\alpha}$-amylase production ability using nuclear transfer method. Hybrids formed between the strains of Saccharomyces fiburigera KCTC 7393 and Saccharomyces cerevisiae KCTC 7049 (tyr-, ura-)were obtained by nuclear transfer technique. Nuclei isolated from the wild type S. fiburigera strain were transfered into auxotrophic mutants S. cerevisiae and selected the hybrids showing an increased starch degrading capability were selected (MN-16). This transformant grew best and produced maximal ${\alpha}$-amylase activity on the medium containing 2% (V/V) soluble starch. ${\alpha}$-Amylase from MN-16 was purified electrophoretically homogenety and its properties were investigated. The enzyme was purified about 10.6 fold with an overall yield 9.7% from the culture medium by ammonium sulfate fractionation. DEAE-Sephacel column chromatography, and Sephacryl S-200 column chromatography. The purified enzyme showed a single band on SDS-polyacrylamide gel electrophoresis. The molecular weight of the ${\alpha}$-amylase was estimated to be 53,000 daltons by SDS-PAGE and by gel permeation chromatography on Sephacryl S-200. The purified enzyme showed the maximum activity at pH 5.5 and 40${\circ}C$. The km value for soluble starch was 2.5㎎/㎖. The enzyme activity increased in the presence of $Ca^{2+}, Co^{2+}, EDTA, Mg^{2+}, Mn^{2+}, Zn^{2+}$, but inhibited by $Cu^{2+}, Fe^{2+}$, and $Ni^{2+}$

• Animal cells and systems
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• v.1 no.2
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• pp.363-370
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• 1997

RNase MRP is a ribonucleoprotein complex with a site-specific endonuclease activity. Its original substrate for cleavage is the small mitochondrial RNA near the mitochondrial DNA replication origin, thus it was proposed to generate the primer for mtDNA replication. Recently, it has been shown to have another substrate in the nucleus, such as pre-S.8S ribosomal RNA in nucleolus. The gene for the RNA component of RNase MRP (MRP RNA) was found to be encoded by the nucleus genome, suggesting an interesting intracellular trafficking of MRP RNA to both mitochondria and nucleolus after transcription in nucleus. In this study, genomic DNA encoding MRP RNA was microinjected into the nucleus of Xenopus oocytes, to analyze promoter regions involved in the transcription. It showed that the proximal sequence element and TATA box are important for basal level transcription; octamer motif and Sp1 binding sites are for elevated level transcription. Most of Xenopus MRP RNA was exported out to the cytoplasm following transcription in the nucleus. Utilizing various hybrid constructs, export of MRP RNA was found to be regulated by the promoter and the 5' half of the coding region of the gene. Interestingly, the transcription in nucleus seems to be coupled to the export of MRP RNA to cytoplasm. Intracellular transport of injected MRP RNA can be easily visualized by whole-mount in situ hybridization following microinjection; it also shows possible intra-nuclear sites for transcription and export of MRP RNA.

• Proceedings of the Korean Aquaculture Society Conference
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• 2003.10a
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• pp.28-28
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• 2003

Viable interspecific hybrids between yellowtail flounder (Pleuronectes ferrugineus, Store.) and winter flounder (Pleuronectes americanus, Walbaum) were produced by artificial insemination of yellowtail flounder eggs with winter flounder sperm. However, mean fertilization rate, hatching success and early survival up to 3 weeks post hatch were significantly lower than those of parental pure cross controls (P<0.01). Overall, cytogenetic traits (karyological analysis and estimation of cellular DNA contents using flow cytometry) of hybrid flounder were intermediate between the two parental species. Microsatellite assay was used to distinguish the parental genomes in the hybrids; in most cases, one allele was specific to each of the parents. Morphometrics assessed by body proportions indicated that hybrids generally displayed a morphology intermediate between the maternal and paternal species. Interspecific hybrids exhibited abnormal and retarded gonad development in both sexes based on histological analysis of gonads from adult fish. The sterility of the hybrids presents a significant advantage for their use in aquaculture, as potential escapees would not be capable of reproducing in the wild and contaminating natural stocks.

• International Journal of Industrial Entomology and Biomaterials
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• v.11 no.2
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• pp.139-143
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• 2005

The implications of temperature (25, 30 and $35^{\circ}C$) and relative humidity (RH; 60, 70 and $80\%$) on the hatching rhythmicity and hatching parameters (percentage and duration) were studied in the silkworm, Bombyx mori L. under natural photoperiod (LD 12 : 12). Disease free layings (DFLs) of two pure silkworm breeds, Pure Mysore (PM, a multivoltine breed) and $NB_4D_2$ (a bivoltine breed), and their hybrid, $PM{\times}NB_4D_2$ were introduced into the experimental conditions on the $3^{rd}$ day of oviposition till completion of hatching. The hatching rhythm was predominantly diurnal under all temperature and humidity conditions, with peaks just after 'lights-on' phase (6 hrs). Extreme temperature and humidity conditions did not alter the hatching rhythmicity, but prolonged the hatching durations, extending it to the next day, coupled with reduced hatching percentage in PM and $PM{\times}NB_4D_2{\cdot}In\;NB_4D_2$, on the other hand, hatching did not extend to the next day. Hatching percentage in this breed, however, reduced below the economic level under high temperature and low humidity conditions. The high temperature and low humidity together, though did not alter the rhythmicity, seems to exert synergetic effect on the hatching percentage and its duration in the silkworm, B. mori.

• Journal of Plant Biotechnology
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• v.45 no.3
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• pp.236-241
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• 2018

Lilium is a perennial bulbous plant belonging to the liriotypes genus. Our aim was to study the phylogenetic relationships of the Lilium family. Two varieties of Lilium ledebourii, 44 varieties of the gene bank, and one variety from the Tulipa family served as the out group. In order to study the diversity between lilium masses, ITS regions were used to design the marker. The results showed that the guanine base is the most abundant nucleotide. Relatively high conservation was observed in the ITS regions of the populations (0.653). Phylogenetic analysis showed that sargentiae and hybrid varieties are older than other varieties of the Lilium family. Also, the location of L. ledebourii varieties (Damash and Namin) was identified in a phylogenetic tree by using the ITS marker. Overall, our research showed that ITS molecular markers are very suitable for phylogenetic studies in the Lilium family.

• Journal of the Earthquake Engineering Society of Korea
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• v.7 no.3
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• pp.1-7
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• 2003

Excitation and system identification are carried out for a full-scale five-story structure to obtain fundamental data which will be used for the design of viscoelastic dampers, The hybrid mass driver(HMD) installed on the fifth floor was employed as external exciter to provide excitation for the building, Each floor response was measured and processed to find out where and how the viscoelastic dampers are located and designed. The sine-sweep and white noise loadings were applied to the structure by the HMD to obtain dynamic characteristics such as natural frequencies, damping ratios, and modes, The identified building was experimentally investigated again with the designed viscoelastic dampers installed at inter-stories to obtain the response behavior in the companion paper.

• Journal of the Korean Geographical Society
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• v.46 no.6
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• pp.738-750
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• 2011

Partly due to the climate changes, flooding occurs more often than before. Particularly high urbanization causes more damages by flooding with unexpected downpours. Recent mobile telecommunication technology can help to reduce the damage by a natural disaster with quick alarming process. This research aimed to implement a flood warning GIS service system based on open source software and mobile hybrid application program with LBS. The developed system utilized location information of mobile clients for smart phone users to get alerted to flooding immediately. This service system would be very useful in urban areas to reduce the flood damages.

• Advances in Computational Design
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• v.5 no.1
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• pp.55-89
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• 2020

For the first time, an accurate analytical solution, based on coupled three-dimensional (3D) piezoelasticity equations, is presented for free vibration analysis of the angle-ply elastic and piezoelectric flat laminated panels under arbitrary boundary conditions. The present analytical solution is applicable to composite, sandwich and hybrid panels having arbitrary angle-ply lay-up, material properties, and boundary conditions. The modified Hamiltons principle approach has been applied to derive the weak form of governing equations where stresses, displacements, electric potential, and electric displacement field variables are considered as primary variables. Thereafter, multi-term multi-field extended Kantorovich approach (MMEKM) is employed to transform the governing equation into two sets of algebraic-ordinary differential equations (ODEs), one along in-plane (x) and other along the thickness (z) direction, respectively. These ODEs are solved in closed-form manner, which ensures the same order of accuracy for all the variables (stresses, displacements, and electric variables) by satisfying the boundary and continuity equations in exact manners. A robust algorithm is developed for extracting the natural frequencies and mode shapes. The numerical results are reported for various configurations such as elastic panels, sandwich panels and piezoelectric panels under different sets of boundary conditions. The effect of ply-angle and thickness to span ratio (s) on the dynamic behavior of the panels are also investigated. The presented 3D analytical solution will be helpful in the assessment of various 1D theories and numerical methods.