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Effect of Temperature, Relative Humidity on Germination and Development of Powdery Mildew (Leveillula taurica) on Pepper and Its Inoculation Method (고추 흰가루병균(Leveillula taurica)의 발아특성 및 접종법)

  • Kim, Dae-Hyun;Park, Jong-Han;Lee, Jung-Sup;Han, Kyung-Sook;Han, You-Kyoung;Hwang, Jeong-Hwan
    • Research in Plant Disease
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    • v.15 no.3
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    • pp.187-192
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    • 2009
  • Pepper powdery mildew, Leveillula taurica is one of the most important pathogens of pepper in the greenhouses and fields in Korea and is becoming a worldwide disease. These experiments were carried out to investigate the optimal germination, disease development conditions, disease incidence and effective inoculation methods of pepper powdery mildew. The incidence of powdery mildew was investigated throughout the country based on the major pepper growing areas in 2009. The average rate of infected leaves ranged from 15.3% to 81.8% in greenhouses and fields. Powdery mildew incidences were more severe in greenhouses than those of fields. The optimal temperature for conidial germination was $25^{\circ}C$ and showed the highest germination at 6 hs after treatment. The range of temperature for germination was $10^{\circ}C$ to $35^{\circ}C$. Temperature of greater than $30^{\circ}C$ and below $20^{\circ}C$ affected the abnormal germination and germ tube elongation. The optimal relative humidity for germination and germ tube elongation was 85% and germination increased as relative humidity increased. Disease development started 8 days after inoculation and showed the highest disease severity at 15 days in greenhouse and field regardless of varieties. Among three different inoculation methods tested the spore dropping method was better than touching with infected leaves and spraying with spore suspension. However, the method has limitation in mass inoculation due to the amount of time consumed.

Effects of No-Tillage on Pepper Growing in the Greenhouse with Organic Soil (시설 고추 무경운 유기재배 효과)

  • Yang, Seung-Koo;Seo, Youn-Won;Kim, Hyun-Woo;Kim, Byeong-Ho;Kim, Yong-Sun;Im, Gyeong-Ho;Kim, Hong-Jae;Kim, Jeong-Geun
    • Proceedings of the Korean Society of Organic Agriculture Conference
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    • 2009.12a
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    • pp.287-287
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    • 2009
  • 우리나라의 시설재배면적은 51,237ha에 이르며 수경재배 면적을 제외하면 대부분 경운재배를 하고 있는 실정이다. 경운 작업은 대형 농기구를 이용하여 경운하기 때문에 시설내 토양 입자가 단립으로 잘게 부서지며 대형농기구로 인하여 토양이 답압되고 경운층 아래 경반층이 형성되어 뿌리 발육과 배수 불량의 원인을 제공하게 된다. 경운은 기존에 설치되어 있는 관수시설과 지주, 멀칭을 제거한 후 유기물을 투입하고 2~3회 경운작업 후 다시 소형농기구를 이용하여 이랑을 조성한다. 그리고 제거된 관수시설, 멀칭, 지주를 다시 설치하여야 하기 때문에 시설의 경운재배는 가장 고통스러운 악성노동을 수반하게 된다. 따라서 본 시험에서는 시설 고추 유기재배 시 무경운재배 효과에 대하여 검토하고자 전남 나주시 남평읍 평산리 "JD 중동통 미사질양토"에서 시험을 수행하였다. 2009년 3월 11일 "녹광" 고추 품종을 재식 거리 135${\times}$38cm로 정식하여 7월 27일까지 재배한 후, 시험포를 1/2로 나누어 트렉터를 이용하여 경운작업을 실시하고 2009년 8월 1일 억제작형으로 "녹광" 고추 품종을 정식하였다. 그리고 나머지 시험포 1/2은 전작물을 재배한 이랑에 경운작업을 생략하고 전작물 고추의 식물체는 지제부를 절단하여 분해되도록 고랑을 피복하였으며, 고추 포기와 포기 사이에 무경운으로 고추를 정식하여 시험을 수행하였다. 고추 정식 전 7월 30일에 경운 시험포에 10a당 29톤의 물을 관수하였으며, 무경운 시험포에는 10a당 48톤의 물을 관수하고, 관수 2일 후 시험포의 습도와 이랑에 발생되는 균열수를 조사하였다. 이랑의 수분함량은 표토 0.23%, 토양 30cm 깊이의 수분함량은 0.18%로 경운방법에 따라서 차이가 없었으나 토양 20cm 깊이의 수분함량은 경운은 0.25%, 무경운은 0.14%로 경운재배 토양에 비하여 뿌리의 주요 분포지역인 작토층의 수분함량이 44% 정도 낮았다. 그리고 경운재배 이랑에서는 7~35cm 정도 되는 균열이 m당 22개정도 발생되었으나 무경운에서는 관찰되지 않았다. 시설 토양의 생물다양성 지표가 되는 미소동물의 분포를 조사한(9월 16일) 결과 경운재배 토양에서는 보리톡톡기 등 8종 97개체가, 무경운 토양에서는 10종 101개체가 포획되었다. 경운 방법에 따른 시설 유기재배 고추의 수확과수는 주당 139~141과, 수량은 1,367~1,431g/주가 수확되고 10a당 수량은 경운재배가 2,790kg, 무경운재배는 2,666kg이 생산되어 경운방법에 따른 수량 차는 인정되지 않았다. 경운방법에 따른 경영비를 분석한 결과 무경운은 경운재배에 비하여 10a당 유기질 비료비 178천원, 대농구상각비 211천원, 노력비 261천원의 절감으로 경운재배 경영비 4,815천원/10a 대비 약 14%의 경영비가 절감되었다. 그리고 작기마다 반복되는 경운작업과 관수시설, 멀칭, 지주설치 작업을 1회 설치 작업으로 연속사용이 가능하여 시설재배에서 가장 고통스러운 악성노동의 회피가 가능하였다.

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Adaptation Strategy in Dry Matter and Seed Production of Rice and Weed Species (벼와 잡초(雜草)의 건물생산(乾物生産) 및 종자생산(鐘子生産) 특성(特性)에 따른 환경적응(環境適應) 전략(戰略))

  • Kim, Soon-Chul;Moody, Keith
    • Korean Journal of Weed Science
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    • v.9 no.3
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    • pp.183-200
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    • 1989
  • An experiment was conducted at the greenhouse of the International Rice Research Institute in 1987 to find out the character of dry matter production, the potential seed production ability and the ecological and physiological strategies to adaptation. For these, two rice cultivars, IR64(lowland rice) and UPLRi-5(upland rice), and seven weed species were used ; Echinochloa glabrescens Munro ex Hook. f., E. cress-galls ssp. hispidula (Retz.) Honda, E. colons (L.) Link, Monochoria vaginalis (Burin. f.), Ludwigia octovalvis Jacq.) Raven, Fimbristylis miliacea Vahl and Cyperus difformis L. Adaptation strategies of weed species varied by species. However, they had efficient seed production strategy through different ways. In general, sedge weed species (F. miliacea and C. difformis) produced great amount of seeds at the expense of seed size through greater ratooning ability and low relative dry weight for flowering. For broadleaved weed species, greater number of descendants were obtained through high plasticity and low relative dry weight for flowering (M. vaginalis) or greater growth ability through effective photosynthetic efficiency (L. octovalvis). Grass weed species, on the other hand, produced their seeds through effective growth (net assimilation rate and relative growth rate), high ratooning ability (except E. crus-galls ssp. hispidula) or low relative dry weight to maximum dry weight for flowering (E. glabrescens). The harvest indices of the weed species were considerably lower than those of rice. Fimbristylis miliacea had the greatest ratooning ability followed by C. difformis, E. colons and E. glabrescens. The greatest seed productivity was recorded by C. difformis (279, 000) and L. octovalvis (268,000) while rice produced the least number of seeds (1300-6100). Log seed weight had a negative linear relationship with log seed number (y=6.30-1.48X, $R=-0.965^{**}$). For all species plant plasticity response was not directly correlated with mortality response.

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Investigation of the Utilization of Organic Materials and the Chemical Properties of Soil in the Organic Farms in Korea (국내 유기농재배지 유기물 시용실태 및 토양의 화학적 특성)

  • Lee Yong-Hoan;Lee Sang-Guei;Kim Sung-Hoan;Shin Jae-Hoon;Choi Doo-Hoi;Lee Yun-Jeong;Kim Han-Myeng
    • Korean Journal of Organic Agriculture
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    • v.14 no.1
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    • pp.55-67
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    • 2006
  • A survey of 31 organic farmers were conducted to investigate the actual conditions of organic matter application. The amounts of organic matter application in the fields were higher in order of fruit, vegetable and rice farm. The average was 50 ton/ha in vegetable farms. In the green vegetable farms saw dust and animal manure were mainly utilized to make compost. Rice straw, wood chip, and forest bushes were also used for composting. In the fruit vegetable farms materials relatively lower in nitrogen content such as rice straw and cattle manure were used in vegetative period and materials higher in nitrogen content such as oil cake and wild grass were used in reproductive phase. Nutrient balance investigated in the farm in Icheon region who produce lettuce, angelica, and kale continuously in one cropping year indicated surplus in three major nutrients. Nitrogen and phosphorous were in excess by 29 and 10 kg respectively in the organic rice farm in yang-pyoung region. While soil chemical properties in the organic farms are within the adequate range in open field, it is much higher than the limits in the greenhouse soils. Overall application of organic matter is in an oversupply state. This results suggested that the organic matter management should be based on the soil conditions for sustainable cultivation. Chemical composition of organic matters and soil test reports should be considered prior to the application of organic matter.

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Studies on the Herbicidal Properties of Pyrazolate (제초제(除草劑) Pyrazolate의 작용특성(作用特性)에 관한 연구(硏究))

  • Ryang, H.S.;Han, S.S.;Kim, K.H.
    • Korean Journal of Weed Science
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    • v.3 no.2
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    • pp.174-189
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    • 1983
  • Experiments were conducted to evaluate the herbicidal characteristics of pyrazolate [4-(2,4-dichloro benzoyl)-1,3-dimethylpyrazol-5-yl-p-toluene-sulphonate] in greenhouse and lowland rice field. Pyrazolate controlled effectively most of annual weeds and such perennial weeds as Sagittaria pygmaea MIQ., Potamogeton distinctus A. BENN, Sagittaria trifolia L., Cyperus serotinus ROTTB, and Scirpus hotarui OHWI., whereas Eleocharis kuroguwai OHWI. was tolerent to pyrazolate. Although pyrazolate was applied at 2 to 10 days after transplanting, there was no difference in weed control The weeding effect was not influenced by percolation, depth of water and soil type. No difference in crop injury of rice was found with various levels of seedling age, transplanting depth, percolation, depth of water, soil type and time of application. When combined with butachlor, the mixture gave the same effect on rice phytotoxicity and weed control as pyrazolate alone did. Pyrazolate moved 1 to 2cm downward in lowland soil regardless of soil type and percolation. The herbicidal activity of pyrazolate persisted in soil for 60 to 90 days, depending on soil type, percolation and presence of soil microorganism.

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Acetolactate Synthase Activity Inhibition and Herbicidal Activity of Sulfonylurea and Imidazolinone Herbicides (Sulfonylurea 및 imidazolinone계 제초제(除草劑)의 살초작용(殺草作用)과 acetolactate synthase 활성(活性) 억제작용(抑制作用))

  • Hwang, I.T.;Hong, K.S.;Cho, K.Y.
    • Korean Journal of Weed Science
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    • v.15 no.1
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    • pp.54-62
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    • 1995
  • Acetolactate synthase activity inhibition and herbicidal activities were investigated with 2 sulfonylureas [chlorsulfuron{2-chloro-N-{{(4-methoxy-6-methyl-1,3,5-triazin-2-yl) amino} carboxyl} benzenesulfonamide}, metsulfuron-methyl{methyl-2{{{{(4-methoxy-6-methyl-1,3,5-triazin-2-yl)amino}carbonyl}amino}sulfonyl}benzoic acid}, and 2 imidazoli-nones [imazethapyr{2-{4,5-dihydro-4-methyl-4-(1-methyl)-5-oxo-1H-imidazol-2-yl}-5-ethyl-3-pyridinecarboxylicacid}, imazaquin{2-{4,5-dihydro-4-methyl-4-(1-methyl)-5-oxo-1H-imidazol-2-yl}-3-quinoline carboxylic acid} herbicides. A broad weeding spectrum was observed with the treated herbicides at low application rates. Both corn(Zea mays L.) and sorghum(Sorghum bicolor Moench) were very sensitive to the two herbicide groups. Although legumes, such as soybean(Glycine max Merr.), clover(Trifolium repense L.), and indian jointvetch(Aeschnomene indica L.) were sensitive to the sulfonylureas, they were tolerant to the imidazolinones. On the contrary, wheat(Triticum aestivum L.) and barley(Hoderum sativum Jess.) showed the reverse responses of the legumes to the two herbicide groups. Quackgrass(Agropyron repens(L.) P. Beauv.). however, was commonly tolerant to the two herbicide groups. Degrees of crop injury and acetolactate synthase inhibition also varied with the crops examined. The 50% inhibition concentrations of sulfonylureas on acetolactate synthase in vitro activity($IC_{50}$) from corn, wheat, and soybean did not relate to the greenhouse herbicidal activities ($GI_{50}$). With chlorsulfuron, for example, wheat had more than 100 times higher $GI_{50}$ than corn and soybean, but the $IC_{50}$ was 4 to 10 times lower. Similar observation was made with metsulfuron-methyl. However, closer relationships between $IC_{50}$ and $GI_{50}$ were found with the imidazolinones. When imazethapyr was applied, the order of $GI_{50}$ values against com, wheat, and soybean was the same as that of $IC_{50}$.

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Effect of Rice Seed Disinfection of Loess-sulfur on the Suppression of Bakanae disease caused by Fusarium fujikuroi (벼 키다리병 방제에 관한 황토유황의 종자소독 효과)

  • So, Hyun-Kyu;Kim, Yong-Ki;Hong, Sung-Jun;Han, Eun-Jung;Park, Jong-Ho;Shim, Chang-Ki;Kim, Min-Jeong;Kim, Seok-Cheol
    • Korean Journal of Organic Agriculture
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    • v.25 no.2
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    • pp.345-355
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    • 2017
  • This study was conducted to evaluate rice seed disinfection efficacy of loess-sulfur for the suppression of Bakanae disease caused by Fusarium fujikuroi. Rice seeds were treated at different concentrations of loess-sulfur, soaking time and temperature, and combination of hot-water treatment. Rice cultivar, Shindongjin harvested from Bakanae disease-infested area in 2015, was used. Loess-sulfur was treated as follows; concentration of undiluted solution, 2%, 1% and 0.5%; soaking time of 24 and 48 hours; treatment temperature of $20^{\circ}C$ and $30^{\circ}C$; hot water treatment or not. Optimal conditions of rice seed disinfection were selected soaking time of 48 hours and the suspension of 0.5% and 1% loess-sulfur by investigating seed germination and isolation frequency of Fusarium spp. on Komada agar medium in vitro, and were established 3 disinfection conditions as hot water ($60^{\circ}C$, 10 min.) + 1% loess-sulfur ($20^{\circ}C$, 48 hours), 1% loess-sulfur only ($30^{\circ}C$, 48 hours) and 1% loess-sulfur only ($20^{\circ}C$, 48 hours) through additional test in greenhouse. Above 3 conditions were verified by rice seedling box and paddy field test in the way of investigating Bakanae diseased plants (%) and healthy plants (%). Consequently, most effective rice seed disinfection conditions on Bakanae disease were combination of hot water and 1% loess-sulfur and loess-sulfur only at $30^{\circ}C$. Furthermore, treatments with these conditions showed control value of 100% were maintained from seedling to the heading stage in the field. However, treatment of 1% loess-sulfur only at $20^{\circ}C$ showed low control value of 78.2% in paddy field. Hot water only treatment turned out to be an effective disinfection method when conducted thoroughly with $60^{\circ}C$, 10 min. However, it was thought additional soaking process with loess-sulfur after hot water treatment served more high control effect against Bakanae disease when rice seeds were disinfected on a large scale. This results expected rice seed disinfection with loess-sulfur were effectively and easily usable method if farmers had only one of either hot water-disinfector or seed-disinfector. In addition, loess-sulfur is well-known to farmers, simple to manufacture method and cheap.

Changes of Tomato Growth and Soil Chemical Properties as Affected by Soil pH and Nitrogen Fertilizers (토양 pH와 질소 관비 비종에 따른 토마토 생육 및 토양화학성 변화)

  • Kang, Yun-Im;Roh, Mi-Young;Kwon, Joon-Kook;Park, Kyoung-Sub;Cho, Myeong-Whan;Lee, Si-Young;Lee, In-Bok;Kang, Nam-Jun
    • Korean Journal of Environmental Agriculture
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    • v.29 no.4
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    • pp.328-335
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    • 2010
  • This study was conducted to determine effects of soil pH and form of nitrogen fertilizers on tomato growth and chemical properties of greenhouse soil using ferigation system. Tomato (Lycopersicon esculentum Mill. cv. Superdoterang) were grown for three months in 18 L pots filled with two soil (pH 6.8 and pH 8.7). 4 different nitrogen fertilizers (urea, ammonium nitrate, ammonium sulfate, and potassium nitrate) were fertigated with different concentrations of 0, 10, 50, and 100 mg N/L during tomato cultivation. Soil pH 8.7 decreased yield and chlorophyll fluorescence compared with soil pH 6.8. Yield at soil pH 8.7 increased by ammonium nitrate and ammonium sulfate fertigation. Soil pH 6.8 induced increment of yield by nitrogen concentration than form of nitrogen fertilizers. Soil pH after cultivation of tomato decreased by application of ammonium nitrate and ammonium sulfate. Soil EC by 100 mg N/L application of ammonium sulfate was twice as much as other fertilizers. Form of nitrogen fertilizer had less effect on concentration of soil $NH_4^+$-N and $NO_3^-$-N in soil but the concentrations slightly reduced at pH 8.7. These results indicate that application of urea and ammonium nitrate for a nitrogen source of fertigation has little affects on soil chemical properties before and after tomato cultivation.

Effect of Root Zone Warming by Hot Water on Rhizosphere Environment and Growth of Greenhouse- grown Oriental Melon (Cucumis melo L.) (온수 지중가온이 참외의 근권환경 및 생육에 미치는 영향)

  • 신용습;이우승;도한우;배수곤;최성국
    • Journal of Bio-Environment Control
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    • v.6 no.2
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    • pp.103-109
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    • 1997
  • This experiment was conducted to investigate the effects of root zone warming on rhizosphere temperature of Oriental melon (Cucumis melo L. var. Makuwa) in winter season. Root zone was warmed by hot water flowing through pipe set at 35cm depth from the ridge. Treatments of minimum soil temperature at 20cm depth were 17, 21, $25^{\circ}C$, and non-warmed from Jan. 18 to Apr. 18. The results are summarized as follows. 1. The cumulative soil temperature for 1 month after planting oriental melon was 441, 558, 648, and 735$^{\circ}C$ at control, 17, 21, and $25^{\circ}C$ plot, respectively. 2. As soil temperature was higher, air temperature in tunnel was higher. The lowest temperature in control plot at night was 9.5$^{\circ}C$, 11.$0^{\circ}C$ in 17$^{\circ}C$ plot, 13.5$^{\circ}C$ in 21$^{\circ}C$ plot, and 16.5$^{\circ}C$ in $25^{\circ}C$ plot, respectively. 3. The xylem exudate amount of control plot for 24 hours just after basal stem abscission was 8.1$m\ell$. It was 1.2 times higher in 17$^{\circ}C$ plot, 1.3 times higher in 21 $^{\circ}C$ plot, and 4.8 times higher in $25^{\circ}C$ plot than in control plot at 30 days after planting. The xylem exudate amount at 67 days after planting of control plot was 10.4$m\ell$, those of 17, 21, $25^{\circ}C$ plots were 1.1, 3.2, and 3.3 times as compared to control plot. 4, Early growth in leaf length, stem diameter, leaf number and leaf area for 30 days after planting were better in higher temperature plots than in control plot. Particularly, the increase of leaf area was striking in higher temperature plots. Leaf area of control plot was 279.5$\textrm{cm}^2$ for 30 days after planting, 153.4% in 17$^{\circ}C$ plot, 745.6% in 21$^{\circ}C$ plot and 879.4% in $25^{\circ}C$ plot were increased as compared to in control plot.

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Habitat Utilization Change of Crane Species against the Increasing Anthropogenic Structure after Released from Civilian Control Zone; CCZ in Cheorwon, Rep. of Korea (철원 민간인통제지역 해제 이후 인공시설물 증가에 따른 두루미류 분포변화)

  • Yoo, Seunghwa;Joo, Sungbae;Lee, Kisup;Kim, Suho;Kim, Dongwon;Kim, Hwajung;Kim, Jinhan
    • Journal of Environmental Impact Assessment
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    • v.29 no.1
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    • pp.1-7
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    • 2020
  • This study aims to evaluate the effect of artificial facilities constructed afterthe release of the civilian controlled zone (CCZ) in Cheorwon on the inhabitation of cranes. The study site was released from the CCZ in 2012, and several barns have been built since 2017. The average number of Red-crowned Cranes was 9.3±4.3 individuals (±Standard Deviation) in the period before the release of the CCZ from 2009 to 2012, and it decreased by ca. one third of Red-crowned Crane's average individuals were showing 3.5±0.5 individuals after release. The average number of White-naped Cranes also appeared to be decreased by ca. 90% from 63.3±24.6 to 6.0±6.0 individuals. This results suggest that the construction of greenhouses and barns after the releasing of the CCZ affected the decrease of the population of these two crane species. In the case of Red-crowned Crane, the core area tended to move away from the area where barns were built. However, the distribution range of Red-crowned Cranes did not change significantly we expected because the study area was not their favorite place since 2012 when the area was released from the CCZ. The density of the White-naped Crane was relatively high even in the area where greenhouses were built, but after 2017 when the barn was built intensively, core habitat of crane became shrink and fragmented. These results suggest that both Red-crowned Cranes and White-naped Cranes response sensitively against habitat change due to the construction of barns. Since the release of the CCZ, the construction of the greenhouse has been influenced on the distribution of cranes, but subsequently, the construction of the barn seems to have caused a bigger change in their core habitat because the passage of people to barns is more frequent than the greenhouses.