• Asian Journal of Turfgrass Science
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• v.26 no.1
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• pp.35-43
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• 2012

These studies were carried out to ascertain the effects of paraffin oil on prolongation of the green leaf color period in Zoysiagrass. Fifty-fold diluted 98% paraffin oil treatment obtained the lowest reduction rate of green color. In early October we sprayed 50-fold, 100-fold and 200-fold diluted 98% paraffin oil in a Zoysia japonica field in Hapcheon of Gyeongnam and Sangju of Gyeongbuk and in a Z. matrella field in Gumjung of Busan and Anseong of Gyeonggii. Leaf color maintained for 60 days after treatment in Zoysia japonica. Leaf color remained for 60 days after treatment in Anseong, Hapcheon and Sangju when treated in the mid-October. Chlorophyll content was higher in paraffin oil treatment areas than in control areas in early October. Weight reduction rate was lower in the paraffin oil treated than in untreated leaves of Zoysiagrass when sprayed in Gumjung and Anseong in early October and mid-October, respectively. The chlorophyll content in leaves of Z. japonica was increased by increasing the spray volume of paraffin oil in the same dose treated in Lexington, Kentucky, USA. Paraffin oil can be used to prolongate of Zoysiagrass leaf color.

• Asian Journal of Turfgrass Science
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• v.19 no.2
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• pp.131-140
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• 2005

This study was conducted to compare cold tolerances and growth rates of 5 basic species, commercial lines, and breeding lines of zoysiagrass. Total 41 zoysiagrasses were tested at the northern part (Jeokseong-myeon) and the middle part (Cheonan) of South Korea. Cold tolerance (survival rate during winter), growth rate, green-up speed, and density data were collected visually. Four types of zoysiagrasses (Z. matrella, Z. tenuifolia, USm, and S4M2) with genetic characteristics of Z. matrella died during winter cold, while the others survived. Midium leaf type zoysiagrasses, such as Anyang1, Samdeock1, Anyang2, SJ21, and Pyeongdong, with genetic characteristics of both Z. sinica and Z. macrostachya showed vigorous growth rate one year after planting at the northern part (Jeokseong-myeon). Twenty nine ($70.7\%$) zoysiagrasses showed vigorous growth rate, but the other twelve ($29.3\%$) zoysiagrasses died by cold and drought condition at the middle (Cheonan) part of S. Korea. Anyang2, AJ9-7, Samdeock1, Samdeock2, Samdeock3, and Zenith-C showed vigorous growth rate even though cold and drought environmental condition were artificially provided at these region. But, SJ2-19, NSm, 88Mey, 88Mey-7S, 88Mey-9S, SJ2l-10, ASm, and DBm which did not die at the northern part, showed serious injury at the middle part of S. korea. From this results, we can conclude that drought is as important factor as low temperature for zoysiagrasses during winter.

• Asian Journal of Turfgrass Science
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• v.24 no.2
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• pp.88-96
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• 2010

Zoysiagrass, especially Jungji implicating medium-leaf type zoysiagrass has been widely used in South Korea recently. This study was carried out to classify 36 selected medium-leaf type zoysiagrass accessions compared to 5 basic zoysiagrass species using RAPDs. Morphological characteristics such as leaf width, leaf angle, leaf sheath length, existance of trichomes and stolon color were measured as useful characteristics for identification of species in Zoysia genus. Nineteen RAPD markers were identified using 8 selected random primers. The dissimilarity coefficient of variants ranged from 0 to 0.736. Three zoysiagrass groups were clustered by dissimilarity coefficient analysis. Group 1 consisted of Z. japonica and some US varieties including 'Zenith' and 'Meyer'. Group 2 consisted of Z. sinica, Z. macrostachya and Korean commercial varieties such as 'Anyang', 'Samdeock', and 'Pyeongdong' medium-leaf type grasses. Group 3 was genetically distinct from Group 1 and Group 2, and included Z. matrella and Z. tenuifolia. 'Anyang', 'Samdeock', and 'Pyeongdong' medium-leaf type zoysiagrasses showed very close genetic relationship with Z. sinica and Z. macrostachya.

• Asian Journal of Turfgrass Science
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• v.11 no.4
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• pp.311-320
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• 1997

This study was carried out to induce and maintain callus from 59 zoysiagrass lines, to know the effective disinfestation method for zoysiagrass stolon as explant and the difference in the response of callus induction among 59 lines, and to investigate the effect of medium, growth regulators, light, temperature, stolon part and internode position on callus induction and emhryogenic callus(E.C.) formation. The treatment of 0.lmg/L $HgCl_2$for 15 min resulted in no contamination and the highest callus induction(46.6%). Callus was induced from the 59 zoysiagrass lines. The callus growth of Z. japonica and Z. sinica was generally better than Z. matrella Ten cell lines whose callus and stolon grow fast in culture and in field, respectively were selected to he used for breeding. Callus induction was the most effective at 2.0mg /L of both 2, 4-D and picloram in MS medium. MS medium was the best for callus induction and growth while LS medium was the best for embryogenic callus and shoot formation. Callus induction and growth was better at 28, 31$^{\circ}C$. than 25$^{\circ}C$. and dark condition was better than light condition in MS me-dium containing 2mg/L 2,4-D. While callus induction was better with node part as explant than with internode part, callus growth and embryogenic callus formation was better with internode part. In 'Japonica 1', the first internode was the most effective in callus induction, but third internode was the best in '$M_2$ X $S_2$'.

• Asian Journal of Turfgrass Science
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• v.15 no.2
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• pp.39-50
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• 2001

This research was designed to develop the rapid propagation technology of zoysiagrass using carpet production and to provide turf-growing farmers with efficient cultivation methods thor carpet production. Turfgrass cultivar tested in cadet production by seeding was Z. japonica cv. Zenith. Since the surface coverage rate reached to 85% in 2 months after seeding at the rate 6g/$m^2$ in the early part of July, carpet production using ‘Zenith’seeds would be possible to produce within within the same year. Thrfgrasses tested in carpet production by vegetative propagation were Z. japonica, medium type Z. matrella, fine type Z. matrella, and medium type Z. japonica. Planting rates of vegetative parts (sprigs) were 1.2L/$m^2$, 2.4L/$m^2$, 2.5L/$m^2$, and 5L/$m^2$. Two different sizes of sprig were used; 1~2 nodes and 3~4 nodes. Surface coverage rate was 90% in one year after planting at the rate of 2.5L sprigs of medium type Z. japonica. Therefore, the use of sprigs with 3~4 nodes at the planting rate of 2.5L would be suitable for the carpet prodcution. Three months old zoysiagrass carpet (Zenith) was overseeded with Kentucky bluegrass, perennial ryegrass, and tall fescue at two different overseeding rate. Surface coverage rate was the highest (80%) at the plot overseeded with perennial ryegrass that showed the earliest germination. Suitable overseeding species for the rapid sod formation at the early stage of establishment were tall fescue and perennial ryegrass and desirable overseeding time was from early to middle of September.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.48 no.1
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• pp.20-24
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• 2003

Proline is known as an osmotrotectant to enhance tolerance against both salt and dehydration stresses. A P5CS ($\Delta^1$-pyrroline-5-carboxylate synthetase) plays a major role in regulation of synthesis of proline. An overexpression of the mothbean P5CS gene in transgenic tobacco plant increased the levels of proline and osmotolerance. In an attempt to look for the possibility to use content of proline as well as a level of P5CS gene expression as molecular markers for salt tolerance, the amounts of proline and transcript levels of P5CS were measured as functions of either concentration of NaCl or length of treatment period among different species of zoysiagrass. Hybridzoysia showed the highest level of proline ($329\mu\textrm{g}$/g.f.w.) among five different species of zoysiagrass at 250 mM NaCl in 24 hours. The level of P5CS transcript was also the highest in the hybridzoysia at 250 mM NaCl in 24 hours. The transcriptions of P5CS gene were induced at the rates of 1.2, 1.2, 1.8, and 1.8, upon treatment of 250 mM NaCl in Z. japonica, Z. matrella, Z. sinica and hybridzoysia respectively. Based on a correlation between the level of P5CS transcript and the proline content among different species of zoysiagrass, a comparative structural analysis of the gene for P5CS from either Z. sinica or hybridzoysia may lead to an understanding of mechanism for salt tolerance shown differently among zoysiagrasses.

• Journal of The Korean Society of Grassland and Forage Science
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• v.26 no.4
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• pp.267-276
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• 2006

To investigate the physiological responses to winter freezing stress naturally occurring, the level of lipid peroxidation and enzymatic antioxidant responses were compared between zoysiagrass and creeping bentgrass during overwintering. Root mortality of creeping bentgrass was significantly higher than zoysiagrass at January. Root growth of creeping bentgrass was nearly parallel with temperature fluctuation, while zoysiagrass showed little changes in root growth until the end of April. Total nonstructural carbohydrate of zoysiauass was 10% higher than creeping bentgrass. Malondialdehyde(MDA) content in creeping bentgrass was 2-fold higher than that of zoysiagrass. The peroxidase(POD) activity of creeping bentgrass in January was 4.2 times higher, while superoxide(SOD) and catalase(CAT) activities lowered 22% and 67%, respectively, compared to zoysiagrass. These results suggest that zoysiagrass roots much properly operate cold tolerance mechanism and: are less susceptible to cold stress in comparison to creeping bentgrass.

• Weed & Turfgrass Science
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• v.5 no.4
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• pp.274-279
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• 2016

Aphanisticus congener is a newly recorded buprestid (Coleoptera) insect pest of turfgrass in Korea. This buprestid pest was initially found from turfgrass conservation site in a greenhouse in Jinju, Gyeongnam province, Korea in July, 2014. The Aphanisticus in the family Buprestidae is a leaf miner. A. congener is the close species of A. aeneus which was firstly reported as sugarcane leaf sucker in India. A. congener was active from early July to late August in the greenhouse. Damage by the insect led to drying out and browning of turfgrass leaf because larva fed on cell sap of leaves and adult fed on leaf surface. A. congener damaged Zoysia japonica, Z. sinica, Conodon dactylon, and Poa pratensis when adults were artificially released into potted turfgrasses in the laboratory. In green house, A. congener damaged Z. japonica, Z. macrostachya, Z. matrella, Z. sinica, Conodon dactylon, and hybrid zoysiagrass. However, no damage symptoms were observed from the same turfgrass accessions in the nearby field of the greenhouse. Thus, the new coleopteran pest may be a warm-adapted pest for turfgrass, damaging turfgrass leaf only in warmer conditions.

• Asian Journal of Turfgrass Science
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• v.20 no.1
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• pp.1-10
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• 2006

This study was carried out to develope new zoysiagrass cultivar 'Millock'(Patent registration No. : 10-2005-0110051). Artificial selfing of collected line of MJ8 was conducted to develope F1 plant (MJ8S). Among the inbred progenies, MJ8S-9 (Millock) showed superior performance in color, density, and rust resistance. 'Millock' showed genetically dark green color, with medium-textured leaf ($4.2{\pm}0.44$ mm), short internode length ($3.5{\pm}0.28$ cm), and wide leaf angle ($52.5{\pm}10.8$ degree). Height to the lowest leaf blade of this cultivar was $1.9{\pm}0.91$ cm, which may allow low mowing height. 'Millock' has a yellowish green stolen. Also, specific bands with primer number OPB 8 by RAPD analysis can be used for a cultivar identification.

• Asian Journal of Turfgrass Science
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• v.25 no.2
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• pp.229-236
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• 2011

This study was carried out to provide recent information on zoysiagrass (Zoysia spp.) sod production in Japan. Total zoysiagrass sod production acreage in Japan was 6,623 ha. The acreage by prefecture was 3,900 ha (58.9%) in Ibalaki, 779 ha (11.8%) in Tostoli, 609 ha (9.2%) in Kagosima, and 550 ha (8.3%) in Sijeumoka, respectively. Sod was harvested by 1.5 cm sod thickness. Mowing height of Goraisiba (Zoysia matrella) and Nosiba (Zoysia japonica) cultivars was cut by 10 mm and 25 mm height, respectively. Japan agricultural cooperative issued a warranty of sod production quality to zoysiagrass consumers. A quality evaluation for sod production was carried out continuously to maintain a reasonable degree of uniformity and hight quality of sod production three times a year. Also, zosiagrass sod production was rotated with Chinese cabbage (Brassica rapa var. glabra Regel) for improving fertility of soil every five to six years. Patented cultivars for promoting bland in Ibaraki prefecture of Japan were 'Tsukuba himae', 'Tsukuba kagayaki', 'Tsukuba Talou' and 'Tsukuba green'.