• 한국식물병리학회지
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• 제13권4호
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• pp.239-243
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• 1997

1990년 8월 충북 보은의 참깨 밭에서 전형적인 엽화병(葉化病; phyllody disease)이 발생하였다. 병징은 꽃이 잎처럼 녹색으로 변하고, 이병엽은 정상엽보다 작고 잔가지가 마치 빗자루 모양으로 총생하였고 개화 및 결실이 되지 않았다. 투과 전자현미경으로 병든 잎의 사관요소에서 phytoplasma가 존재하는 것을 확인하였다. 병든 잔가지에서 추출한 DNA를 중합효소 연쇄반응으로 분석한 결과 대추나무 빗자루병에 걸린 나무에서 증폭되는 DNA와 같은 크기의 band가 관찰되었다. 따라서 위의 시료는 phytoplasma 벙으로 확인 되었으며 참깨 엽화병으로 명명하였다. 또한 참깨 엽화병을 일으키는 phytoplasma와 대추 나무 빗자루병 phytoplasma를 PCR 증폭 및 제한효소로 절단하여 분석한 결과 이들은 서로 다른 그룹이라는 것을 알 수 있었다.

• 한국유기농업학회지
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• 제23권1호
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• pp.67-75
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• 2015

유기멀칭자재가 제초작업에 소요되는 노동력을 절감하고 대추 유목의 생장에 미치는 영향을 구명하고자 2012년부터 2013년까지 4종류의 유기 멀칭자재를 이용하여 시험을 수행하였다. 시험에 이용된 멀칭재료(볏짚, 왕겨, 톱밥 및 우드칩)는 2012년에 대추 유목이 심겨진 토양위에 처리되었다. 2012년 모든 멀칭재료별 잡초 발생 억제율은 96~98% 범위에 있었다. 2013년에 잡초 발생 억제율은 볏짚멀칭 처리구에서 7%, 왕겨멀칭 처리구에서 60%, 톱밥멀칭 처리구에서 27% 그리고 우드칩멀칭 처리구에서 79%이었다. 2013년 잡초 발생 억제율은 2012년에 비하여 감소하였다. 대추 유목의 생육은 우드칩멀칭에서 가장 양호하게 나타났으며, 왕겨멀칭에서 가장 저조하게 나타났다.

• 한국식물병리학회지
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• 제14권5호
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• pp.402-407
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• 1998

Phytophthora rot on jujube fruit has occurred at several cultivation areas in Kyung-buk and Kyung-nam provinces. Symptoms consisted of brownish to reddish rot on fruits resulting in early drop or mummification. The causal fungus isolated from infected fruits and adjacent leaf stalks was identified as Phytophthora citricola, which has never been reported in Korea. Sporangia were semi-papillate, noncaducous and highly variable in shapes. Plerotic oospores with paragynous antheridia were abundant is single cultures. Sporangia of two isolates were measured as 38-76$\times$20-40 ${\mu}{\textrm}{m}$ and averaged 51.4$\times$27.0 and 55.6$\times$36.0 ${\mu}{\textrm}{m}$. Oogonia were ranged from 26 to 36 ${\mu}{\textrm}{m}$ and averaged 31.3 and 32.0 ${\mu}{\textrm}{m}$. Colony pattern was slightly radiated with sparse aerial mycelia on common media. Minium, optimum and maximum temperatures for mycelial growth were recorded at 7, 25, and 32$^{\circ}C$, respectively. Among tested media, 10% V8A was the best and $25^{\circ}C$ was better than 15$^{\circ}C$ for oospore formation of the fungus. The jujube isolates of P. citricola were readily differentiated from other closely related species in the genus, namely; P. nicotianae, P. citrophthora, P. cactorum, P. capsici, and P. plalmivora on the basis of PCR-RFLP of r-DNA. The fungus showed strong pathogenicty to jujube, apple, pear, orange, persimmon and eggplant, and relatively weak to citron, tomato, pepper and cucumber. In this study, P. citrocola is firstly identified and jujube fruit rot caused by the fungus is recorded as a new disease in Korea.

• 동의신경정신과학회지
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• 제23권2호
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• pp.1-12
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• 2012

Objectives : This study is set out to discover more about the treatment of anxiety disorder in Traditional Chinese Medicine. Methods : We searched the database-China Academic Journals(CAJ). For papers published since 2007-2011, which are classified in categories. Results : We have finally selected 23 articles, and the results were as follows: 1. Stages of Anxiety disorder are discussed in various aspects. 2. Diagnostic criteria and evaluation criteria were the same, especially, in the CCMD and HAMA was used most frequently. Adverse effects were reported using TESS. 3. There are various ways, such as Herbal medicine, acupuncture, electroacupuncture stimulation, acupoints injection, and abdominal massage therapy to treat Anxiety disorders in TCM. 4. Zizyphus jujuba, Poligala tenuifolia, Poria cocos are mainly used in herbal medicine. Sinmun(HT7), Sameumgyo(SP6), Naegwan(PC6) are mainly used in acupuncture points. 5. The treatment group, cured by herbal medicine or acupuncture, was similar in its therapeutic efficiency to that of the control group, which was cured by western medicine. In some cases, the treatment group was better off than the control group. Herbal medicines have been reported that they have less side effects and drug addiction than the western medicines. We predict that herbal medicine in the treatment for anxiety disorders will have an advantage. Conclusions : There are a wide range of approaches in the treatments for Anxiety disorders in TCM. In some aspects, herbal medicine is more effective than that of the western medicine. According to this study, we predict that herbal medicine in the treatment for anxiety disorders, has some advantage. Therefore, there is a need for more clinical research for this disorder in Korean traditional medicine.

• 한국식품영양학회지
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• 제14권5호
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• pp.405-410
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• 2001

영지, 표고, 눈꽃동충하초 그리고 번데기동충하초를 이용한 버섯젤리의 제조와 제품의 색도, 물성 및 기호도를 비교 조사하였다. 버섯 젤리 제조를 위한 추출액의 적정혼합비율을 선발한 결과, 영지버섯 젤리는 버섯추출액 85%, 대추추출액 10%, 황기추출액 5%를 혼합했을 때, 표고버섯 젤리는 버섯추출액 80%, 대추추출액 10%, 감초추출액 5%, 오미자추출액 5%를 혼합했을 때, 눈꽃동충하초와 번데기동충하초 젤리는 버섯추출액 05%, 대추추출액 10%, 감초추출액 5%를 혼합했을 때 가장 우수하였다. 젤화제 종류별로 버섯 추출액에 대한 응고 효과는 모든 버섯에 대해 carrageenan이 가장 효과적이었다 버섯 젤리의 색도는 carrageenan 첨가량에 따라 큰 차이가 없었고, hardness, gumminess, chewiness는 증가하는 경향이었다. 관능은 젤화가 완전히 이루어지면서 hardness가 낮은 carrageenan 0.6% 첨가가 가장 우수하였다.

• Journal of Plant Biology
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• 제3권1호
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• pp.32-38
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• 1960

Since the peculiar virus disease of chinese date tree (Zizyphus jujuba Mill. var. inermis Rehd.) has been noted in South Korea around 1950, 70% to 80% of the economically important trees have been either completely destroyed or infected with the virus, severe damage has been noted, particularly, across the area ranged from middle east to the middle part of Korea, including Seoul area. Yoon-Koock-Byung in 1958 first reported the disease and descirbed it might be caused by a kinds of yellows. But he did not conform in his paper that the disease is pecisely caused by yellows virus. The authors, hereby intend to identify the true cause of the desease of the chinese data tree by studying the external symptoms of the disease and the internal morphological characteristics of the diseaset plant which shows various abnormalities in contrast to the healthy checks. In view of fact that leaves of the infected plants become yellowish in color similar to the peach yellows, aster yellows, it is likely to be identifiable as the common yellows. Furthermore, the abnormal characteristics observed by the authors are as follow: The floral organs such as petals, sepals, stamens, and pistil turn into vegetative leaves, the leaves on heavily infected plant appear as small sized one and also showing as a common witch's broom like symptom. There are also an occuring of numerous advantitious shoots developed from both of stems and roots. The amount of photosynthetic starch grains increases in parenchymatous cells, necrosis takes place in mesophyll, Particularly, Palisade Parenchyma in the leaves of infected plants are distinguished in contrast to the healthy checks. From the symptoms and the present experimetns described above, the authors are believed that the disease of chinese data tree is not caused by the yellows. It appears the disease is rather similar to the symptoms of sandal spike virus which was noted in India early in this centry. But the host plant of standal disease, Santalum albun L. and the insect vector, Jassus indicus Wal., have never been reported in Korean flora and the founa. The termperature and the otehr environmental factors is quite different Korea and India. Thus the authors believe that the peculiar disease must be an endemic new virus origin in Korea and must be called as "shoot cluster disease of chinese date tree."

• 한국식품과학회지
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• 제37권6호
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• pp.997-1004
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• 2005

여러 천연물 추출물 140종의 파골세포 분화처제효과 검색은 파골세포의 마커 enzyme인 TRAP활성으로 확인하였다. 그 결과 파고지$^{3}$, 지각$^{13}$, 금령자$^{6}$, 시호$^{9}$, 황연$^{69}$, 배양인삼$^{98}$, 구맥$^{26}$, 등 대부분의 추출물이 $100{\mu}g/mL$ 농도 이상에서는 높은 세포 독성을 보여서 파골분화 억제 정도를 확인 할 수 없었고 세포독성이 없는 농도를 선정하여 파골세포 분화억제 정도를 재검색한 결과는 천연물 중 파고지$^{3}$, 호두$^{21}$, 용안육씨$^{77}$, 황금$^{78}$, 정공등$^{37}$ 메탄올 추출물과 생대추과육$^{128}$, 콩나물$^{70}$ 열수 추출물은 비교적 낮은 농도에서도 30% 이하의 TRAP 활성을 보여 파골분화 억제 효과가 우수하게 나타났다. 이 중 전통 한약재인 황금$^{78}$ 메탄올 추출물과 과일류인 생대추과육$^{128}$ 열수 추출물이 $10{\mu}g/mL$의 낮은 농도에서도 가장 좋은 파골분화 억제 효과가 나타났다.

• Journal of Radiation Protection and Research
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• 제26권1호
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• pp.27-33
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• 2001

Apoptosis의 유발을 억제하는 한약제를 파악하여 apoptosis와 관련된 정상적 또는 질병에 대한 연구에 자료를 제공하고자 방사선에 의해 유도된 apoptosis를 지표로 한의학적 처방에서 많이 사용되는 대표적 한약제(24종)의 효과를 검증하였다. 용안육, 산조인, 원지, 인삼, 복령 목향, 천궁 백작약, 승마, 시호 및 눈꽃동충하초 투여군에서 apoptosis는 감소되었으며, 이들 생약제는 apoptosis와 관련된 질병의 예방 및 치료에 적용할 수 있을 것이다. 추후 이들 생약의 작용 기전에 대한 연구가 요구된다.

• 한국식품영양과학회지
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• 제28권3호
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• pp.698-704
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• 1999

In this study, we evaluated the radioprotective effects of Kuei Pi Tang and its ingredients, as a prescription of traditional Oriental medicine. The jejunal crypt survival, endogenous spleen colony formation, and apoptosis in jejunal crypt cells were investigated in mice irradiated with high and low dose of ${\gamma}$ rays. In the mice administered with Kuei Pi Tang extract before irradiation, the jejunal crypts were protected significantly(p<0.005) and the frequency of radiation induced apoptosis was reduced(p<0.05). In the experiment on the effects of the ingredients of Kuei Pi Tang, the results indicated that the extract of Dangui(Angelica sinensis), Suanzaoren(Zizyphus valgaris), Rensan (Panax ginseng), Fuling(Poria cocos) and Muxiang(Saussurea lappa) might have a major radioprotective effect. Although the mechanisms of this inhibitory effect remain to be elucidated, these results indi cated that Kuei Pi Tang might be a useful radioprotector, especially since it is a relatively nontoxic natural product.

• 미생물학회지
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• 제3권1호
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• pp.1-6
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• 1965

Over a period of 1962-64, a transmission-experiment of witchess' broom of jujube tree by stem-grafting was conducted. When stem-grafting of sound scions upon diseased roots or diseased scions upon sound roots were carried out, disease transmission of high rate was witnessed; 99% in the former and, in the latter, 62% of the stocks which saw union by callussing and had new shoots. Even when the diseased scions by stem-grafting or the diseased buds by budding upon sound stock died away, the transmission rate was 21% in stem-grafting and 14% in budding which seems to show that, when tissues of diseased plants and stocks are kept contacted over a certain period, the disease transmission occurs. And when the recovered scions taken from once diseased tree were grafted upon diseased roots, the transmission rate was 100 % and therefore it is presumed that the immunity could not be acquired even under the assumption of complete recovery from the disease. In stem-grafting of the diseased scions upon sound roots, 98% of the scions which were stored in the cellar, overwintered and grafted in spring was diseased, whereas the disease rate of the scions which were cut and grafted in spring was only 33%. It was particularly noteworthy that 90% of the scions in the former case and only 3% in the latter case were diseased as of June 18th approximately 2 months after the actual grafting and then the latter advanced to 33% with the passage of time. It appears that the pathogen in branches and shoots of the diseased trees standing outdoors become inactivated or diminished during winter. Through its symptom, pathological change in tissue, and easy transmission of the disease via stem-grafting, it seems certain that the pathogen of the witches broom disease in jujube tree is a virus.