• Proceedings of the Computational Structural Engineering Institute Conference
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• 2006.04a
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• pp.733-740
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• 2006

Inverted V (or chevron) braced steel frames have been seen as being highly prone to soft story response once the compression brace buckles under earthquake loading. To salvage chevron braced frames. the concept of the zipper column was proposed many years ago such that the zipper column can redistribute the inelastic demand over the height of the building. However. rational design method for the zipper column has not been established yet. In this paper, a new dynamic design method for the zipper column was proposed by combining the refined physical braced model and modal pushover analysis. Inelastic dynamic analysis conducted on 6 story building model showed that the proposed method was more superior to the existing static design method and was very effective in improving seismic performance of chevron braced steel frames.

• Structural Engineering and Mechanics
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• v.73 no.3
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• pp.303-317
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• 2020

In this study an innovative rocking zipper braced frame (RZBF) is proposed to overcome the deficiencies of common concentrically braced frames. RZBF is an improved rocking concentrically braced frame which is based on combination of rocking behavior and zipper columns. The base rocking joints and post-tensioned bars provide rocking response and restoring force, respectively. Also, zipper columns distribute the unbalance force over the frame height and reduce the damage concentration. To evaluate seismic performance of RZBF, a comparison study is carried out considering concentrically braced frame, zipper braced frame, rocking concentrically braced frame and RZBF. Thereby, a suite of non-linear time history analyses had been performed on four different types of archetypes with four, six, eight, ten and twelve stories. Frames were designed and non-linear time history analyses were conducted in OpenSees. To compare the seismic behavior of the archetypes, roof drifts, residual roof drifts, story drifts, the forces of first and top story braces, PT bars forces, column uplift and base shears were taken in to consideration. Results illustrate that using RZBF, can reduce the damage due to reduced residual drifts. Zipper columns enhance the seismic performance of rocking systems. As the number of stories increase in the RZBF systems, larger top story braces were needed. So the RZBF system is applicable on low and midrise buildings.

• Journal of the Earthquake Engineering Society of Korea
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• v.8 no.3
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• pp.53-62
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• 2004

In this paper the overstrength factors, ductility factors, and response modification factors of ordinary concentric braced frames designed in accordance with a current seismic design code are determined by performing pushover analysis. According to the analysis results, the overstrength and the response modification factors turn out to be larger than the values regulated in the codes in most model structures. However if the braces are reinforced by BRB or zipper columns, the overstrength factors and response modification factors turn out to increase significantly.

• Journal of Life Science
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• v.30 no.11
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• pp.1012-1020
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• 2020

Remicade is a therapeutic biosimilar natural antibody in which the mouse variable domain has been linked to the human constant domain. It is a chimeric monoclonal antibody specific to tumor necrosis factor-alpha (TNF-α) and has been developed for the treatment of rheumatoid arthritis. To investigate the biological activity of the Remicade antibody, we carried out a bioinformatics study using a protein data bank to characterize the TNF-α antigen binding mechanism of the Remicade natural antibody. Because the production of the Remicade antibody is often limited by genetic instability of the natural antibody-producing cell, we generated a Remicade single-chain variable domain fragment antibody (Remicade) in which a heavy chain variable domain (VH) is joined with a light chain variable domain (VL) by a polypeptide linker. Furthermore, Remicade was fused to a leucine zipper (RemicadeScZip) for higher production and higher antigen-binding activity than Remicade. The Remicade and Remicade ScZip were expressed in Escherichia coli and purified by a Ni⁺-NTA-agarose column. As expected, the purified proteins had migrated as 28.80 kDa and 33.96 kDa in sodium dodecyl sulfate-polyacrylamide electrophoresis. The TNF-α antigen binding activity of Remicade was not observed by ELISA and western blot. In contrast, RemicadeScZip showed antigen-binding activity. Additional bio-layer interferometry analysis confirmed the antigen-binding activity of RemicadeScZip, suggesting that the leucine zipper stabilized the folding of RemicadeScZip in a denatured condition and improved the TNF-α antigenbinding activity.

• Journal of Korean Association for Spatial Structures
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• v.19 no.4
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• pp.45-52
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• 2019

The purpose of the paper is to introduce a system that reduces the occurrence of weak-story in the event of earthquake. Weak-story concentrates deformation on the story and causes all member to collapse before the capacity of all member is reached. This paper introduces Strong-Back system (SB) to protect weak story. SB is a hybrid of zipper frame, tied eccentrically braced frame, and elastic truss system and it is divided into elastic and inelastic areas. Elastic areas prevent the generation of weak story by distributing energy, and inelastic areas dissipate energy through buckling or yielding. In this paper, the seismic performance is evaluated by comparing the four type braced frame with SB through push-over analysis. The four criteria are compared from the base shear, the ductility capacity, the column failure order, and the quantity of brace. As a result, SB proved to have sufficient performance to protect the weak-story.

• Journal of Life Science
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• v.15 no.6 s.73
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• pp.928-934
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• 2005

ATF2 is a cellular transcription factor which belongs to the CREB/ATF class and it is leucine zipper protein which generally binds to DNA as dimers. This paper presents the procedure for subcloning the ATF2 gene and the results of experiment used the expressed ATF2. The pET expression vector was used since it produced 6xHis fusion protein for easy purification using affinity column. The Nickel chelating chromatography was used for Purifying the expressed ATE2 from E- codi BL2l. Subsequen시y In vitro binding pull-down assay showed the binding specificity of ATF2 with AP-1 family factors such as BATF, c-Fos, c-Jun and ATF2 itselgf. ATF2 forms homodimer as well as strong heterodimer with BATF. It also forms stable dimer with c-Jun but barely binds with c-Fos.