• Molecules and Cells
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• 제38권5호
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• pp.416-425
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• 2015

NF-E2-related factor 2 (Nrf2), a basic leucine zipper transcription factor, has recently received a great deal of attention as an important molecule that enhances antioxidative defenses and induces resistance to chemotherapy or radiotherapy. In this study, we investigated the apoptosis-inducing and Nrf2- upregulating effects of quercetin on malignant mesothelioma (MM) MSTO-211H and H2452 cells. Quercetin treatment inhibited cell growth and led to upregulation of Nrf2 at both the mRNA and protein levels without altering the ubiquitination and extending the half-life of the Nrf2 protein. Following treatment with quercetin, analyses of the nuclear level of Nrf2, Nrf2 antioxidant response element-binding assay, Nrf2 promoter-luc assay, and RT-PCR toward the Nrf2-regulated gene, heme oxygenase-1, demonstrated that the induced Nrf2 is transcriptionally active. Knockdown of Nrf2 expression with siRNA enhanced cytotoxicity due to the induction of apoptosis, as evidenced by an increase in the level of proapoptotic Bax, a decrease in the level of antiapoptotic Bcl-2 with enhanced cleavage of caspase-3 and PARP proteins, the appearance of a sub-$G_0/G_1$ peak in the flow cytometric assay, and increased percentage of apoptotic propensities in the annexin V binding assay. Effective reversal of apoptosis was observed following pretreatment with the pan-caspase inhibitor Z-VAD. Moreover, Nrf2 knockdown exhibited increased sensitivity to the anticancer drug, cisplatin, presumably by potentiating the oxidative stress induced by cisplatin. Collectively, our data demonstrate the importance of Nrf2 in cytoprotection, survival, and drug resistance with implications for the potential significance of targeting Nrf2 as a promising strategy for overcoming resistance to chemotherapeutics in MM.

• 한국의류학회지
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• 제30권8호
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• pp.1178-1187
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• 2006

The purpose of this study is to conduct a questionnaire survey on the actual conditions of the working uniform and protective equipment put on by street cleaners, then to identify their inconvenience and problems and to draw solutions to improve them, and finally to design a spring-and-autumn street cleaner uniform which has enhanced functionalities such as motional flexibility, safety and soil proof. The questionnaire survey of this study was done by street cleaners who were in charge of street cleaning and collecting the recyclable waste. The result of this study, easily worn-out parts of the working clothing were the sleeve hems and the front part on the upper uniform, and the trouser hems and zipper on the lower uniform. Easily soiled parts were the sleeve hems, parts of arm on the upper uniform, and the trouser hems and knee parts on the lower uniform. Based on the results of the questionnaire survey, the sample uniforms were designed in consideration of material, design and pattern. A soil-proof material was used for easily soiled parts such as sleeve hems, knee parts and trouser hems. Reflective material was applied to the parts such as chest, back and the side lines of sleeves and trousers to raise visibility in working and to ensure safety. A crease was given to each side of the back. The height of sleeve cap was lowered to lift the motional flexibility of back and arm parts. Darts were applied to elbow and knee parts for a three-dimensional design. Gussets were inserted in the thigh part to give it activity. Then quantitative evaluation and a sensory evaluation, which was composed of outward appearance, motional flexibility and fitness for field operation, were conducted. According to these evaluations, the sample uniform was found to be more satisfactory than current uniform.

• 한국의류학회지
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• 제33권3호
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• pp.420-432
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• 2009

The purpose of this study is to survey the present women's golf windbreaker market to develop the trendy product with supplementing design and functionality by grasping the inconvenient part through the result of the questionnaire. According to the questionnaire, 38.1% said that the armhole is the most inconvenient part by tightfitting. Therefore, a mobility of wearer was enhanced by giving shirring detail to the armhole for flexibility through inserting the elastic band with a regular interval around armhole in the design1. The swing movement was allowed to be convenient by attaching knit to the side panel. And, the sleeves were designed in the detachable form so those are allowed to be produced in two kinds such as long sleeves and short sleeves. In the design2, a zipper was inserted from the neckline to the armhole so it is designed a detachable bolero. Furthermore, it was designed to be possible to wear two different way as a jumper style and a vest style. Given wearing a vest, the sleeve part was patched knit, thereby having enhanced activity. The design1 and the design2 evaluated an objective assessment and subjective assessment. Assessment group consists of 5 subject groups and 10 expert groups to evaluate external appearance and movement adaptability. In the external appearance evaluation, the design1 obtained the better evaluation than design2. In the movement adaptability evaluation, the design1 obtained the better evaluation than the design2 for all of the items except the wrist area. In the group of professionals, the design1 obtained the better evaluation than the design2 for all of the items except the bottom area.

• 천문학회보
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• 제36권2호
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• pp.129.1-129.1
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• 2011

We have applied an advanced multi-aperture indexing photometry and sophisticated de-trending method to existing Taiwanese-American Occultation Survey (TAOS) data sets. TAOS, a wide-field ($3^{\circ}{\times}3^{\circ}$) and rapid photometry (5Hz) survey, is designed to detect small objects in the Kuiper Belt. Since TAOS has fast and multiple exposures per zipper mode image, point spread function (PSF) varies in a given image. Selecting appropriate aperture among various size apertures allows us to reflect these variations in each light curve. The survey data turned out to contain various trends such as telescope vibration, CCD noise, and unstable local weather. We select multiple sets of stars using a hierarchical clustering algorithm in such a way that the light curves in each cluster show strong correlations between them. We then determine a primary trend (PT) per cluster using a weighted sum of the normalized light curves, and we use the constructed PTs to remove trends in individual light curves. After removing the trend, we can get each synthetic light curve of star that has much higher signal-to-noise ratio. We compare the efficiency of the synthetic light curves with the efficiency of light curves made by previous existing photometry pipelines. Our photometric method is able to restore subtle brightness variation that tends to be missed in conventional aperture photometric methods, and can be applied to other wide-field surveys suffering from PSF variations and trends. We are developing an analysis package for the next generation TAOS survey (TAOS II) based on the current experiments.

• 복식문화연구
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• 제20권2호
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• pp.222-237
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• 2012

Air-containing fashion, which can offer diverse functions through the inflow and outflow of air, is highly relevant in today's mobile society, where people are experiencing a wider range of environments. This study attempts to suggest the possibility of air-containing multi-functional fashion that could continuously be utilized by developing a design for an air-containing jacket using modular systems. In this research, the modular systems in architecture and furniture design were referenced through a review of the literature for the purpose of establishing modular systems in fashion. Functions relevant to the mobility of today's society are derived from the results of advanced research and applied to the design of modules of the jacket. The modules are integrated through the modular systems. The folding and unfolding structure in architecture and furniture is applied as a folding system in fashion, the vertical accumulation structure as a layering system, and the horizontal integration structure as a combining system, and in addition, the containing system has emerged in fashion. Each module is designed to fulfill certain functions, such as cushioning, heat insulation, and portability. The folding system is utilized in designing the cushion module to support the neck and back of a wearer by making its hood and hem fold in the back. The application of a layering system was suggested by making the vest, combined with the neck cushion and back cushion via the combining system, layered with its insulation module. By applying the combining system, the hood that includes the neck cushion, the skirt that includes the back cushion, the body that includes the insulation module, and the sleeves can be connected and separated by a zipper. The applicability of this concept was proven by applying a developed design to an actual item.

• 한국균학회소식:학술대회논문집
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• 한국균학회 2016년도 춘계학술대회 및 임시총회
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• pp.27-27
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• 2016

The ascomycete fungus Fusarium graminearum is the most common pathogen of Fusarium head blight (FHB), a devastating disease for major cereal crops worldwide. FHB causes significant crop losses by reducing grain yield and quality as well as contaminating cereals with trichothecenes and zearalenone (ZEA) that pose a serious threat to animal health and food safety. ZEA is a causative agent of hyperestrogenic syndrome in mammals and can result in reproductive disorders in farm animals. In F. graminearum, the ZEA biosynthetic cluster is composed of four genes, PKS4, PKS13, ZEB1, and ZEB2, which encode a reducing polyketide synthase, a nonreducing polyketide synthase, an isoamyl alcohol oxidase, and a transcription factor, respectively. Although it is known that ZEB2 primarily acts as a regulator of ZEA biosynthetic cluster genes, the mechanism underlying this regulation remains undetermined. In this study, two isoforms (ZEB2L and ZEB2S) from the ZEB2 gene in F. graminearum were characterized. It was revealed that ZEB2L contains a basic leucine zipper (bZIP) DNA-binding domain at the N-terminus, whereas ZEB2S is an N-terminally truncated form of ZEB2L that lacks the bZIP domain. Interestingly, ZEA triggered the induction of both ZEB2L and ZEB2S transcription. In ZEA producing condition, the expression of ZEB2S transcripts via alternative promoter usage was directly or indirectly initiated by ZEA. Physical interaction between ZEB2L and ZEB2L as well as between ZEB2L and ZEB2S was observed in the nucleus. The ZEB2S-ZEB2S interaction was detected in both the cytosol and the nucleus. ZEB2L-ZEB2L oligomers activated ZEA biosynthetic cluster genes, including ZEB2L. ZEB2S inhibited ZEB2L transcription by forming ZEB2L-ZEB2S heterodimers, which reduced the DNA-binding activity of ZEB2L. This study provides insight into the autoregulation of ZEB2 expression by alternative promoter usage and a feedback loop during ZEA production.

• 생명과학회지
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• 제22권9호
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• pp.1201-1206
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• 2012

Crlz1 유전자는 B 세포 분화 과정 중 pre-B 세포 분화 단계에서 특이적으로 발현됨이 밝혀져 있다. Crlz1 유전자의 발현과 연관되는 3개의 DNase I hypersensitive site (HSS8, 9, 10)가 Crlz1 유전자의 전사 시작점으로부터 바로 위쪽의 chromatin 상에서 발견되었고, 그 중에서 HSS9/10은 Crlz1 promoter 지역에 해당하며 매우 높은 전사 활성을 가지는 것으로 이미 보고 되었다. 본 연구에서는 앞의 연구에서 더 나아가 HSS8이 Crlz1 promoter의 전사활성을 약 2 배 증가시키는 enhancer의 역할을 하고 있는 현상을 밝혔으며, 또한 HSS8의 위치가 Crlz1 유전자의 전사 시작점을 기준으로 하였을 때 -1055와 -1159 사이, 즉 104 bp 길이의 chromatin DNA 상에 존재하며, 이러한 HSS8의 위치는 luciferase reporter의 활성 측정으로 비교 분석되어진 deletion construct들의 전사 활성에 대한 결과와도 잘 부합되었다.

• 한국통신학회논문지
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• 제33권12C호
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• pp.971-982
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• 2008

본 논문에서는 무선 전송 시스템에서 OFDMA(Orthogonal Frequency Division Multiple Access) 방식을 사용하여 상향링크 신호와 하향링크 신호를 동시에 전송하면서 자원의 효율을 높이고 자원 할당의 유연성을 향상시킬 수 있는 디지털 이중화 방식으로 SDD(Synchronous Digital Duplexing) 방식을 제안한다. SDD 방식은 상 하향링크 신호를 동시에 송 수신하기 위해 상호 레인징(mutual ranging) 과정을 통하여 AP(access point)와 각 SS(subscriber station), SS와 다른 SS 사이의 상호 시간 동기 정보와 상호 채널 정보를 획득한다. 획득한 정보는 전송시간 선택 및 CS 삽입 여부 판단, CS 길이 설정, FFT 구간 재설정 등에 이용된다. 제안된 SDD 방식이 무선채널에서 사용되는 기존의 이중화 방식인 TDD(Time Division Duplexing), FDD(Frequency Division Duplexing)와 비교하여 옥내 환경에서 적합한 이중화 방식임을 확인한다.

• 한국산학기술학회논문지
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• 제7권6호
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• pp.1100-1105
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• 2006

칼라코드는 획득된 영상에서 칼라의 심각한 왜곡 때문에 그 응용 확장에 어려움이 있었다. 칼라 인식에서 칼라 왜곡의 영향을 줄이기 위해서는 규칙적으로 샘플링된 몇 개의 화소들을 이용하기 보다는 가능한한 각 칼라 영역에서 많은 화소들을 통계적으로 처리하는 것이 더 바람직하다. 이를 위해서는 일반적으로 에지 검출이 필요한 분할이 필요하다. 그러나, 칼라코드에서 에지들은 분할을 불완전하게 만드는 지퍼 효과나 반사와 같은 다양한 왜곡에 의해 끊어질 수 있고, 에지 연결 또한 어려운 처리 과정이다. 본 논문에서는 칼라 인식에서 칼라 왜곡의 영향을 줄이기 위한 좀 더 효과적인 방법은 분할을 위한 정확한 에지 검출을 배제하는 방법으로 k-평균 클러스트링 알고리즘을 적용하였다. 또한, 칼라코드 검출에서 6개의 안전한 칼라와 그레이 성질 모두 이용하였다. 실험은 4M-화소 크기의 야외영상 144장에 대해 수행되었다. 제안한 방법은 테스트 영상에 대해서 100%의 칼라코드 검출율을 나타내었고, 검출된 코드에 대해서는 99% 이상의 평균 칼라 인식 정확도를 보였다. 여기서 가장 높은 정확도를 보인 캐니 에지 검출법을 사용한 경우 91.28%로 나타났다.

• 생명과학회지
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• 제15권6호
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• pp.928-934
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• 2005

ATF2는 c-Fos와 c-Jun과 같은 전사인자이며 이들은 루신지퍼 단백질이다. 루신지퍼 단백질은 동형이합체 혹은 이형이합체를 형성하며 promoter 영역에 결합하여 전사조절에 중요한 역할을 한다. 세포의 전사인자 ATF2는 ATF/CRE site에 결합하며 특히 선택적인 interfamily 이형이량체를 형성함으로써 전사 조절에 있어서 다양한 메카니즘을 제공할 수 있다. 본 연구에서는 ATF2 cDNA를 6xHis를 가진 expression vector에 subcloning하여 E.cnli BL2l에서 발현시켰다. 6xHis tag은 nickel-chelating chromatography를 가능하게 하였다 발현된 ATF2는 In vitro binding pull-down assay에서 동형이합체를 이를 뿐만 아니라 AP-1 그룹의 인자들과 선택적인 이형이합체를 형성함을 보여 주었다. BATF와는 강하게 결합하였으며 c-Jun과도 안정된 이합체를 형성하였다. 그러나 c-Fos와는 이합체를 형성하지 않으므로서 AP-1그룹 내에서도 이합체 형성이 선택적으로 이루어짐을 알 수 있다.