• 대한수의학회지
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• 제36권2호
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• pp.471-482
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• 1996

These experiments were undertaken in order to find out the useful clinicopathological diagnostic methods of cadmium poisoning in dogs. Twenty-one dogs were divided into a control group and 6 experimental groups. The experimental groups were adminstered orally 5, 10, 15, 30, 60 and 120mg of cadmium per kg of body weight for 56 days. All dogs were examined for clinical signs, and weekly changes in hematological and blood chemical values. All dogs were necropsied on 57th days of experiment. Tissue samples including hair, skin, muscle, lung, liver, kidney, spleen, pancreas, testis, ovary, uterus, and bone were collected and analyzed for cadmium, zinc, iron and copper contents using atomic absorption spectrophotometer. From these experiments following results were obtained : 1. All experimental dogs showed vomitting, salivation, anorexia, decreased water-intake, dehydration, and marked weight loss. The dogs received 30mg/kg or more of cadmium died during the period from 2nd to 7th week after administration. 2. Hematologically, all experimental dogs showed decrease in erythrocyte count, hemoglobin concentration, and packed cell volume. The anemia was identified as normocytic and regenerative morphologically. 3. No significant differences in serum glutamic oxaloacetic transminase, glutamic pyruvic transaminase, blood urea nitrogen, and cholosterol value were obseved between the control and experimental dogs. 4. The cadmium contents in various tissues of experimental dogs were estimated as $37.8{\sim}201.8{\mu}g/g$ in bone, $14.1{\sim}49.5{\mu}g/g$ in liver, $13.2{\sim}53.1{\mu}g/g$ in kidney, $0.4{\sim}35.2{\mu}g/g$ in pancreas, $0.8{\sim}35.4{\mu}g/g$ in spleen, $0.9{\sim}30.1{\mu}g/g$ in hair, $0{\sim}7.1{\mu}g/g$ in lung, $0{\sim}5.1{\mu}g/g$ in skin, and $0{\sim}3.6{\mu}g/g$ in muscle, respectively. However, the serum, testis, ovary and uterus showed no cadmium accumulation. Two contol dogs showed cadmium accumulation only in bone. 5. Significant differances in zinc, iron, and copper contents in tissue samples were observed between the control and experimental groups.

• 한국토양비료학회지
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• 제50권5호
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• pp.401-408
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• 2017

Organic amendments such as animal waste compost, lime-bordeaux mixture, and lime sulphur mixture contain heavy metal(loid)s which are toxic to human being, animal, and plant. The objective of this study was to compare heavy metal(loid)s contamination of soil between conventional and organic farm. Soil samples were collected from 10 conventional and 38 organic fruit farms. At each sampling point, top (0~15 cm) and sub soil (15~30 cm) were taken using hand auger. Total concentration for arsenic (As), cadmium (Cd), copper (Cu), nikel (Ni), lead (Pb), and zinc (Zn) in the collected soil were measured. The pollution index (PI) for heavy metal in organic farms indicated it was unpolluted area. However, mean value of PI for organic farms (0.143) was higher than that for conventional farms (0.122). High Geoaccumulation index (Igeo) for Cu, Pb, and Zn implied that grape farms were more polluted than others fruit farms because a lot of lime-bordeaux mixture and lime sulphur were applied in organic grape farms. Especially, top soils showed higher level of contamination than sub soil. Based on the above results, organic amendments might cause accumulation of heavy metals in soil. Therefore, particular attention should be paid for concentration of Cu, Pb, and Zn, when organic amendments are used in the organic fruit farms.

• 한국환경농학회지
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• 제16권2호
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• pp.124-129
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• 1997

중금속오염폐수처리에 미생물 이용 가능성을 검토하기 위하여 중금속에 강한 내성을 지님과 동시에 균체내 중금속 축적능력이 우수한 중금속 내성균을 분리하여 온도, pH, 및 균체 전배양시간 등과 같은 외부 요인에 따른 균체내 중금속 축적변화를 조사한 결과는 다음과 같다. 중금속화하물 종류에 따른 균체내 중금속 축적량은 $-nitrate(NO_3)$ group을 가진 중금속화합물에서 약간 높았으나 화합물 종류에 따라서 큰 차이는 없었다. 균체 전배양시간에 따른 중금속 축적은 24시간 전배양한 균체에서 높게 나타났으나 전반적으로 전배양시간에 따라서 큰 차이는 없었다. 중금속 내성균의 균체내 중금속 축적은 온도, pH등 외부환경요인에 영향을 받았으며, 중금속 내성균의 중금속 최적축적 온도는 전반적으로 $20{\sim}37^{\circ}C$ 범위였고, 중금속 최적축적 pH는 전반적으로 $6{\sim}8$ 범위였다. 중금속을 처리한 용액중 중금속 내성균의 균체량을 증가시킬수록 균체내 축적된 총 중금속양은 증가되었으나 균체 단위 g당 중금속 축적량은 감소되었으며, 균체량의 증가에 비례해서 중금속 축적량이 증가되지는 않았다.

• 생약학회지
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• 제16권4호
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• pp.181-190
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• 1985

To determine contents of inorganic elements of Ganoderma lucidum, the horn-shaped carpophores and the pileus of Ganoderma lucidum were incinerated and analyzed by inductively coupled plasma atomic emission spectrophotometry. The ash contents of the pileus and the horn-shaped carpophore were 1.48% and 1.40%, respectively. The pileus contained calcium, magnesium, sodium, manganese, iron, zinc and germanium in that order. The horn-shaped carpophore contained magnesium, calcium, zinc, manganese, iron, copper and germanium in that order. To examine the protein-bound polysaccharide from Ganoderma lucidum for immunopotentiating activity, its fruit bodies were extracted with hot water. Purification of the extract was carried out by acetone precipitation and dialysis. The fraction obtained during the purification procedure consisted of a polysaccharide moiety (51%) and a protein moiety (5%). When the compound was administered intraperitoneally to the mice at a dose of 50mg/kg, it enhanced the accumulation of the peritoneal exudate cells, macrophage and polymorphonuclear leucocytes, thereby indicating immunopotentiation.

• BMB Reports
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• 제50권2호
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• pp.85-90
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• 2017

Recently, we demonstrated that superoxide dismutase 3 (SOD3) is a strong candidate for biomedicine. Anti-oxidant function of SOD3 was accomplished without cell penetration, and it inhibited the inflammatory responses via non-enzymatic functions. SOD3 has the heparin binding domain associating cell surface. Interestingly, we found that $Zn^{2+}$ promotes transduction effects of recombinant human SOD3 (rhSOD3) by increasing uptake via the heparin binding domain (HBD). We demonstrated an uptake of rhSOD3 from media to cell lysate via HBD, resulting in an accumulation of rhSOD3 in the nucleus, which was promoted by the presence of $Zn^{2+}$. This resulted in increased inhibitory effects of rhSOD3 on NF-{\kappa}B and STAT3 signals in the presence of $Zn^{2+}$, which shows elevated association of rhSOD3 into the cells. These results suggest that an optimized procedure can help to enhance the inflammatory efficacy of rhSOD3, as a novel biomedicine.

• The Korean Journal of Ecology
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• 제21권3호
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• pp.225-231
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• 1998

The studies on the potentiality of biomonitoring heavy metal pollution in coastal region of industrial complex were performed to investigate the heavy metal accumulation and induction of metal-binding protein (MBP) as detoxification process using Rumex maritimus. Bioconcentration in organs and MBP in root of R. maritimus was investigated for the research of the tolerance of heavy metals. The bioconcentration of cadmium and zinc in organs showed 3.6-8.0 times in root higher than in shoot, so it was found that heavy metal accumulated selectively in root. MBP increased absorbance in 254 nm and decreased in 280 nm, because it was composed of high cystein content and low aromatic acids, so absorbance had large difference between 254 nm and 280 nm. The existence of MBP in the 10-20 fraction was ascertained with anion exchange chromatography and it was identified that concentration of heavy metal increased according as an exposure concentration of medium increased in QAE Sephadex A-25 elution profile. These results suggested that MBP could play a role in biomarker determining the bioconcentration of plant. This study demonstrated a possibility that removal ability of heavy metal of R. maritimus resulted from detoxification process and MBP could be utilized as a biomarker of heavy metal pollution.

• 아시안잔디학회지
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• 제10권4호
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• pp.297-304
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• 1996

The investigation was performed to reveal the removal rate of metal constituents of litters in a Phragmites communis Miseanthus sacchariflorus, Typha angastata and Seirpas tabernaemontani grasslands in the lake Paldangho. The removal rates of metal constituents are determined by the mathematical models. The removal rates and time required to decay up to a percentage of each metal constituent were calculated using these model. The removal rates of Cu, Fe and Zn were 0.61, 0.58 and 0.79 in Phragmites communis 0.39, 0.47 and 0.68 in Miseanthus saccharflorus; 0.26, 0.09 and 0.23 in Typha angustata: 0.56, 0.27 and 0.67 in Seirpus tabernaemontani respectively. The periods required to reach half time to the stedy state of the removal and accumulation for Cu, Fe and Zn were 1,13,1.19 and 0.79 years in Phragmites communis; 1.79, 1.49 and 1.02 years in Miscanthus sacchariflorus; 2.70, 7.43 and 2.96 years in Typha angustata ; 1.23, 2.58 and 1.04 years in Scirqus tabernaemontani, re-spectively. Key words: Phragmites communis, Miscanthus sacchariflorus, Typha angustata and Scirpus tabernaemontani, lake Paldangho, Removal rate, Cupper, Iron, Zinc.

• 한국환경농학회지
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• 제2권1호
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• pp.18-23
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• 1983

아연광산(亞鉛鑛山) 린근답(隣近沓)의 토양중(土壤中) Cd, Pb, 및 Zn의 합량(合量)이 현미중(玄米中) 집적(集積)에 미치는 영향(影響)을 구명(究明)하기 위하여 1979년(年)과 1980년(年)에 현미시료(玄米試料) 93점(點)과 토양시료(土壤試料) 180점(點)을 채취(採取) 분석(分析)한 결과(結果)를 요약(要約)하면 다음과 같다. 1) 토양중(土壤中) Cd, Pb 및 Zn 함량(含量)에 대(對)한 현미중(玄米中)로 함량비(含量比)(상대흡수능(相對吸收能))은 토양농도(土壤濃度)의 역수치(逆數値)에 고도(高度)의 직선회귀관계(直線回歸關係)가 성립(成立)되었으며 년차간(年次間) 차이(差異)의 유의성(有意性)은 납이 가장 크고 다음이 아연(亞鉛)이었으나 카드뮴은 유의성(有意性)이 인정(認定)되지 않았다. 2) Cd, Pb, Zn의 상대흡수능(相對吸收能)은 토양(土壤) pH와 부(負)의 상관관계(相關關係)를 가지나, 유의성(有意性)은 1979년(年)에는 Cd, Zn에서 인정(認定)됨에 반(反)하여 1980년(年)에는 인정(認定)되지 않았다. 3) 년차간(年次間) 상대흡수능(相對吸收能)의 차이(差異)는 재배기술(栽培技術)의 차이(差異)보다 기상요인(氣象要因)(평균기온(平均氣溫), 일조시간등(日照時間等))의 차이(差異)에서 오는 것이 더 컸을 것으로 추정(推定)되었다. 4) 현미중(玄米中) 카드뮴 함량(含量)이 일본식량청(日本食糧廳) 규제농도(規制濃度)인 0.4ppm을 초과(超過)할 수 있는 발생빈도(發生頻度)는 $34{\sim}47%$이며, 현미중(玄米中) 납의 함량(含量)이 국제식품규격상(國際食品規格上) 허용한계농도(許容限界濃度)인 2ppm을 초과(超過)할 수 있는 발생빈도(發生頻度)는 $0{\sim}11%$이었다.

• 한국지하수토양환경학회지:지하수토양환경
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• 제15권3호
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• pp.27-33
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• 2010

The purpose of this study was in search of native plant species showing metal-resistant property and excessively accumulating heavy metals in metal-contaminated soil or abandoned mines as well as in evaluation of applicability of phytoremediation. In the study area, species showing excessively accumulating heavy metals were a shepherd´s purse, pampas grass, a Korean lettuce, a Hwansam vine, the Korean persicary, a foxtail, a goosefoot, and a water pepper. The first screened plant species in Sambo mine were as shepherd's purse, Korean lettuce and pampas grass Among them the shepherd´s purse can be excluded because it is a seasonal plant and has lower removal capacity for heavy metals. The Korean lettuce was also excluded because of having lower removal capacity for heavy metals. Pampas grass is a highly bionic plant species constantly growing from spring. However it has weak points such as little accumulation capacity for zinc as well as small values of an accumulation factor and a translocation factor. Another problem is regarded as removal of roots after the clean up if pampas grass is applied to a farmland. In Sanyang mine, wormwood and Sorijaengi were considered as adaptable species.

• 농업과학연구
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• 제48권1호
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• pp.179-189
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• 2021

Plants need essential mineral elements to favorably develop and to complete their life cycle. Despite the irreplaceable roles of microelements, they are often ignored due to the relative importance of macroelements with their influence on crop growth and development. We focused on the changes in primary metabolites in the leaves and roots of bell pepper plants under 6 microelements-deficient conditions: Copper (Cu), Zinc (Zn), Iron (Fe), Manganese (Mn), Boron (B) and Molybdenum (Mo). Bell pepper plants were grown in hydroponic containers, and individual elements were adjusted to 1/10-strength of Hoagland nutrient solution. A remarkable perturbation in the abundance of the primary metabolites was observed for the Fe and B and the Mn and B deficiencies in the leaves and roots, respectively. The metabolites with up-accumulation in the Fe-deficient leaves were glucose, fructose, xylose, glutamine, asparagine and serine. In contrast, the Mn deficiency also resulted in a higher accumulation of glucose, fructose, xylose, galactose, serine, glycine, β-alanine, alanine and valine in the roots. The B deficiency noticeably accumulated alanine, valine and phenylalanine in the roots while it showed a substantial decrease in glucose, fructose and xylose. These results show that the primary metabolism could be seriously disturbed due to a microelement deficiency, and the alteration may be either the specific or adaptive responses of bell pepper plants.