• Journal of Microbiology and Biotechnology
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• v.25 no.5
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• pp.579-588
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• 2015

Fungi of the genus Pestalotiopsis have drawn attention for their capability to produce an array of bioactive secondary metabolites that have potential for drug development. Here, we report the determination of a polyketide derivative compound, pestalotiollide B, in the culture of the saprophytic fungus Pestalotiopsis microspora NK17. Structural information acquired by analyses with a set of spectroscopic and chromatographic techniques suggests that pestalotiollide B has the same skeleton as the penicillide derivatives, dibenzodioxocinones, which are inhibitors of cholesterol ester transfer protein (CETP), and as purpactins A and C', inhibitors of acyl-CoA:cholesterol acyltransferase (ACAT). Strain NK17 can make a fairly high yield of pestalotiollide B (i.e., up to 7.22 mg/l) in a constitutive manner in liquid culture. Moreover, we found that a putative histone deacetylase gene, designated as hid1, played a role in the biosynthesis of pestalotiollide B. In the hid1 null mutant, the yield of pestalotiollide B increased approximately 2-fold to 15.90 mg/l. In contrast, deletion of gene hid1 led to a dramatic decrease of conidia production of the fungus. These results suggest that hid1 is a modulator, concerting secondary metabolism and development such as conidiation in P. microspora. Our work may help with the investigation into the biosynthesis of pestalotiollide B and the development for new CETP and ACAT inhibitors.

• BMB Reports
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• v.42 no.11
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• pp.752-757
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• 2009

Copper is essential but toxic in excess for aerobic organisms. Yeast transcription factor ACE1 functions as a sensor for copper and an inducer for the transcription of CUP1. In addition, ACE1 can activate the transcription of superoxide dismutase gene (sod1) in response to copper. In this study, we introduced the yeast ACE1 into Arabidopsis and analyzed its function in plant. Under high copper stress, the transgenic plants over-expressing ACE1 showed higher survival rate than the wild-type. We also found that over-expression of ACE1 in Arabidopsis increased the activities of SOD and POD, which were beneficial to the cell in copper buffering. Excess copper would suppress the expression of chlorophyll biosynthetic genes in Arabidopsis, RT-PCR analysis revealed that over-expression of ACE1 decrease the suppression. Together, our results indicate that ACE1 may play an important role in response to copper stress in Arabidopsis.

• Journal of Physiology & Pathology in Korean Medicine
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• v.28 no.3
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• pp.251-262
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• 2014

This article is for understanding dementia with the perspective of Korean Medicine through research on syndrome differentiations of dementia clinically applied and relations between modern diseases and Korean Medicine pattern types of dementia. clinical papers were searched in China Academic Journals(CAJ) of China National Knowledge Infrastructure(CNKI) from 2012 to 2013. Conclusions are as follows. First, dementia was expressed in many ways such as imbecility, stupidity, fatuity, idiocy, vacuity, etc and was related with amnesia, forgetfulness, speech not in the right order, depressive psychosis(quiet insanity), manic psychosis, depression syndrome. Second, prescriptions such as QiFuYin and ZuoGuiWan from JingYueQuanShu, XiXinTang and ZhiMiTang from BianZhengLu, TongQiaoHuoXueTang, XueFuZhuYuTang and BuYangHaiWuTang from YiLinGaiCuo, HaiShaoDan from YiFangJiJie, HuangLianJieDuTang from WaiTaiMiYao were suggested for dementia. Third, syndrome differentiation pattern types of dementia are kidney deficiency and marrow decrease, qi-blood depletion, liver-kidney depletion, spleen-kidney depletion, heart-spleen deficiency as deficiency patterns and effulgent heart-liver fire, ascendant hyperactivity of liver yang, qi stagnation and blood stasis, phlegm turbidity obstructing orifice, phlegm-blood stasis obstructing orifice, intense heat toxin as excess patterns and qi deficiency with blood stasis, yin deficiency with yang hyperactivity as deficiency-excess complex patterns. Major pattern types are kidney deficiency and marrow decrease, phlegm-blood stasis obstructing orifice, qi stagnation and blood stasis, liver-kidney depletion, phlegm turbidity obstructing orifice.

• Steel and Composite Structures
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• v.36 no.2
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• pp.187-196
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• 2020

Double skin composite wall system owns several structural merits in terms of high load-carrying capacity, large axial stiffness, and favorable ductility. A recently proposed form of truss connector was used to bond the steel plates to the concrete core to achieve good composite action. The structural behavior of rectangular high walls under compression and T-shaped high walls under eccentric compression has been investigated by the authors. Furthermore, the influences of the truss spacings, the wall width, and the faceplate thickness have been previously studied by the authors on short walls under uniform compression. This paper experimentally investigated the effect of width-to-thickness ratio on the compressive behavior of short walls. Compressive tests were conducted on three short specimens with different width-to-thickness ratios. Based on the test results, it is found that the composite wall shows high compressive resistance and good ductility. The walls fail by local buckling of steel plates and crushing of concrete core. It is also observed that width-to-thickness ratio has great influence on the compressive resistance, initial stiffness, and strain distribution across the section. Finally, the test results are compared with the predictions by modern codes.

• The Journal of the Korea Contents Association
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• v.16 no.6
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• pp.516-528
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• 2016

Tourism information related to the selection and the images formation of tourist destinations is getting more important in tourism industry, and the problems of information overflow is also on the rise simultaneously. And this information overflow can affect the tourists' cognition, emotion and attitudes about tourism information. The objective of this study is to assess their information overload on tourism information on tourists' emotion, attitude and information searching effort. Data were collected from total 438 people with 224 people at public spaces in Seoul, Korea and 214 people at public spaces in Xi'an, China. This study conducts confirmatory factor analysis, correlation analysis and structural equation modelling to verifying 5 hypothesis related to information overflow. The main results of the study show that the cognition of information overload can lead to positive effect to tourists' positive emotion, attitude and searching effort.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.23
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• pp.10387-10391
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• 2015

Background: This study aimed to identify any association between the p73 gene G4C14-to-A4T14 polymorphism and risk of non-small cell lung cancer (NSCLC) in the south of China. Materials and Methods: We genotyped the p73 gene polymorphism of peripheral blood DNA from 168 patients with NSCLC and 195 normal controls using HRM (high resolution melting) and PCR-CTPP (polymerase chain reaction with confronting two-pair primers). Results: The results of genotyping by HRM and PCR-CTPP were consistent with direct sequencing, the p73 genotype distribution in 168 lung cancer patients being as follows: GC/GC 101 cases (60.1%), GC/AT 59 cases (35.1%), AT/AT 8 cases (4.8%). The carriers of AT/AT genotype had a significantly reduced risk of NSCLC (OR=0.370; 95%CI: 0.170-0.806; p=0.010) as compared with non-carriers. However, we found no relations between p73 genotypes and histological type (p=0.798, $x^2=0.452$), tumor stage (p=0.806, $x^2=0.806$), or lymph node metastasis (p=0.578, $x^2=1.098$). Conclusions: Our findings suggest that the p73 G4C14-to-A4T14 polymorphism may be a modifier of NSCLC susceptibility in the Chinese population.

• Bulletin of the Korean Chemical Society
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• v.32 no.7
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• pp.2253-2259
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• 2011

The self-assembly of one novel organic complex based on chlorogenic acid (HCA) and 2,2'-bipyridine (2,2'-bipy) has been synthesized and characterized. The complex achieved by hydrogen-bonding interactions, adopted a 1:1 stoichiometry in a solid state. The proton transfer occurred from the carboxyl oxygen to the aromatic nitrogen atom to form salts CA${\cdot}$(2,2'-Hbipy), the 2,2'-Hbipy molecule individually occupies the pseudo-tetragonum that is formed with CA. In this paper, the interactions of CA${\cdot}$(2,2'-Hbipy) with bovine serum albumin (BSA) were studied by fluorescence spectrometry. For CA${\cdot}$(2,2'-Hbipy), HCA and 2,2'-bipy, the average quenching constants for BSA were $2.4384{\times}10^4$, $4.653{\times}10^3$, and $3.059{\times}10^3\;L{\cdot}mol^{-1}$, respectively. The mechanism for protein fluorescence quenching is apparently governed by a static quenching process. The Stern-Volmer quenching constants and corresponding thermodynamic parameters ${\Delta}$H, ${\Delta}$G and ${\Delta}$S were calculated. The binding constants and the number of binding sites were also investigated. The conformational changes of BSA were observed from synchronous fluorescence spectra.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.14
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• pp.6123-6128
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• 2015

Background: The aim of this study was to analyze the prognostic implications of pretreatment circulating endothelial cells (CECs) and circulating endothelial progenitor cells (CEPCs) for the survival of patients with lung cancer. Materials and Methods: Relevant literature was identified using Medline and EMBASE. Patient clinical characteristics, overall survival (OS) and progression-free survival (PFS) together with CEC and CEPC positive rates before treatment were extracted. STATA 12.0 was used for our analysis and assessment of publication bias. Results: A total of 13 articles (8 for CEC and 5 for CEPC, n=595 and n=244) were pooled for the global meta-analysis. The odds ratio (OR) for OS predicted by pretreatment CECs was 1.641 [0.967, 2.786], while the OR for PFS was 1.168 [0.649, 2.100]. The OR for OS predicted by pretreatment CEPCs was 12.673 [5.274, 30.450], while the OR for PFS was 4.930 [0.931, 26.096]. Subgroup analyses were conducted according to clinical staging. Odds ratio (OR) showed the high level of pretreatment CECs only correlated with the OS of patients with advanced lung cancer (stage III-IV). Conclusions: High counts of CECs seem to be associated only with worse 1-year OS in patients with lung cancer, while high level of pretreatment CEPCs correlate with both worse PFS and OS.

• Journal of the Korean Society of Costume
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• v.62 no.6
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• pp.80-97
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• 2012

This study empirically examined Seo Yu-gu's views on constumes that are based on 'Boksikjigu[服飾之具]' of Seomyongji[贍用志] in Imwonkyungjeji[林園經濟志]. In 'Boksikjigu', Seo Yu-gu explained different situations and problems that were related to costumes and proposed solutions for the problems. This study examined the contents, and divide the items into caps, accessories, and clothes. The results of this study were as follows. First, Seo Yu-gu in he rited the tradition of erudition. Seo Yu-gu classified various costume items systematically, and explained in detail about the origin, history and materials of the costume items. Second, he emphasized frugality, effective use, and welfare. Seo Yu-gu argued that the sleeves should be shortened for convenience and tried to improve practicality in the functional aspect. Also, he wanted to enrich people's life by improving the convenience of goods used in daily life. Third, he tried to establish identity as a Confucian intellect. This idea was clearly shown in 'costumes'; therefore, they served as evidences that Seo Yu-gu used to correct wrong practices through precise historical investigation and review of Shim-ui[深衣], the symbol of the practice of Jujagarye[朱子家禮]. In his later years, he discovered the plain dress that was worn as a casual wear by ZhuXi[朱熹]and tried to wear it in dailylife.

• Journal of Korean Medical classics
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• v.24 no.6
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• pp.169-186
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• 2011

1. In Sui, Tang and Five Dynasty, a discourse related to sleep was published in various big Fang Shu(方書)'s that later became a valuable historical data. Notably, the importance of good sleep began to be properly discussed in "Bei Ji Qian Jin Yao Fang(備急千金要方)" of 'Sun Si Miao(孫思邈)', etc. 2. During Two Song era, achievements in the previous eras were accepted and diseases related to sleep were explored in more details. 3. In Jin and Yuan(金元) era, Liu He Jian(劉河間), in "Su Wen Xuan Ji Yuan Bing Shi(素問玄機原病式)", recognized 'anger' as the cause for various sleep disorders and Li Dong Yuan(李東垣), in "Pi Wei Lun(脾胃論)", believed 'lethargy' and 'Shi Hou Ji Hun Mao Yu Shui(食後卽昏冒欲睡)' are related to narcolepsy linked with stomach. Zhang Cong Zheng(張從正) observed 'insomnia' as an isolated disease whereas Zhu Dan Xi(朱丹溪), in "Ge Zhi Yu Lun(格致餘論)" argued about somnambulism based on the relationship between Xiang Huo(相火) and Xin huo(心火).