• Proceedings of the Korean Society of Precision Engineering Conference
• /
• 2012.05a
• /
• pp.271-272
• /
• 2012

• Korean Journal of Veterinary Research
• /
• v.49 no.1
• /
• pp.35-38
• /
• 2009

The zebra shark Stegostoma fasciatum which had been reared in the commercial aquaria was found dead and submitted for postmortem examination. A pure bacterial culture was isolated from pale and enlarged liver. The analysis of ureC and 16S rRNA genes confirmed the isolate as Photobacterium (P.) damselae subsp. damselae and this pathogen was sensitive to gentamicin. Although, no mortality in mouse was observed in the experimental infection study, the isolation of this pathogen in aquarium fish is significant because it can act as a reservoir to other aquatic animals and can also be zoonotic potential to human during aquarium management. This paper describes the first isolation of P. damselae subsp. damselae from zebra shark.

• Applied Microscopy
• /
• v.46 no.2
• /
• pp.93-99
• /
• 2016

The thickness of specimen is an important factor in microscopic researches. Thicker specimen contains more information, but it is difficult to obtain well focused image with precise details due to optical limit of conventional microscope. Recently, a microscope unit that combines improved illumination system, which allows real time three-dimensional (3D) image and automatic z-stack merging software. In this research, we evaluated the usefulness of this unit in observing thick samples; Golgi stained nervous tissue and ground prepared bone, tooth, and non-transparent small sample; zebra fish teeth. Well focused image in thick samples was obtained by processing z-stack images with Panfocal software. A clear feature of neuronal dendrite branching pattern could be taken. 3D features were clearly observed by oblique illumination. Furthermore, 3D array and shape of zebra fish teeth was clearly distinguished. A novel combination of two channel oblique illumination and z-stack imaging process increased depth of field and optimized contrast, which has a potential to be further applied in the field of neuroscience, hard tissue biology, and analysis of small organic structures such as ear ossicles and zebra fish teeth.

• Journal of Aquaculture
• /
• v.11 no.3
• /
• pp.353-361
• /
• 1998

Genetic analysis of body pigmentation pattern has been conducted by the reciprocal monohybrid and back crosses using the wild type zebrafish, Danio rerio, and its aquarium morphs (mutants), leopard danio. Also, the effect fo pigmentation mutation was investigated with regard to the survival rates of eggs and larvae for the first 15 days after fertilization. As a result, the pattern pigment distribution was inherited by a single gene having two alleles, and which was basically followed by the principle of dominace and segregaion in Mendelian inheritance. A locus for pigment pattern turned out to be located on an autosomal chromosome. Average survival rates estimated from the various crosses between, and within, wild type zebrafish and leopard danio were as follows ; they were 80.6${\pm}$4.8% from the crosses within leopard danios ($L{\times}L$), 70.6${\pm}$4.2% between leopard female and wild type male ($L{\times}Z$), 73.2${\pm}$2.0% between wild type female and leopard male ($Z{\times}L$), and 83.8${\pm}$6.7% within wild type zebrafish ($Z{\times}Z$). These results indicated that the leopard danio, which were reported as an "aquarium morph" several decades ago and also known as a mutant in pigmentation pattern of the wild type zebrafish, seemed to be genetically stable like the wild type of zebrafish.zebrafish.

• Journal of Veterinary Clinics
• /
• v.27 no.1
• /
• pp.93-96
• /
• 2010

We describe a case of enterolithiasis in an eight-year-old male Grant's zebra (Equus burchelli boehmi) that died after a 10-day history of depression, anorexia, dehydration and colic. On necropsy, an enterolith was discovered at the conjunction of the descending colon and the rectum. The spherical enterolith weighed 1,660 g and was $13.5cm{\times}8cm$ in size. According to scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDS), the nidus consisted primarily of $SiO_2$ with outer layers of magnesium and phosphate. The formation of enteroliths is closely related to diet. We suggest that this captive zebra's diet, which consisted primarily of alfalfa hay with mineral supplements, was the cause of enterolithiasis in this case. This is the first report of enterolithiasis in a captive equid in Korea. Our findings provide information valuable for the development of dietary guidelines to prevent enterolithiasis in captive wild equids.

• Proceedings of the Korean Information Science Society Conference
• /
• 2001.10c
• /
• pp.343-345
• /
• 2001

통신 및 네트워크 사용자의 증가와 실시간 처리를 요하는 멀티미디어 데이터 서비스의 증가의 인하여 네트워크 트래픽이 증가하고 서비스 품질 저하 및 접속 속도의 감소가 초래되고 있다 이러한 문제를 해결하기 위하여 라우터의 용량이 확장되고 있으나, 라우터의 용량확장에 따른 테이블의 크기 증가는 경로 선택을 위한 검색 시간 증가를 초래하므로, 라우팅 테이블의 구성방식에 대한 연구가 필요하다. 본 논문은 TCP/IP 기반의 라우팅 프로토콜을 관리하는 Zebra 소프트웨어를 이용하여, 라우팅 테이블의 구조와 구성방식에 대하여 분석하고, 비교 알고리즘을 제시하여 성능 비교 및 평가를 하고자 한다.

• Journal of Life Science
• /
• v.8 no.4
• /
• pp.426-430
• /
• 1998

The molecular relationships have analyzed between the Bombyx mandarina(wild silkworm) and Bombyx mori strains (domesticated silkworm, geographical silkworms). A total of 166 polymorphic RAPD markers amplified from 35 different primers were used to analyze the molecular relationships among thirteen silkworm strains. The genetic similarity coefficient between Bombyx mandarina and Jam305 showed the lowest genetic similarity value with 0.451, Bombyx mandarina and Bibaekjam showed the highest genetic similarity value with 0.958. These strains were classified into Bombyx mandarina(a wild silkworm) and Bombyx mori(twelve domesticated silkworm) groups upon the genetic similary coefficient of 0.55. Further classificient of 0.60; the 1st sub-group (J111, Bibaekjam, $pnd^{ps}$), the 2nd sub-group (Galwon, C18, od yujam, JAM306, C108), the 3rd sub-group(R-hwang, p50), the 4th sub-group(zebra) and the 5th sub-group(JAM305). According to this study, RAPD markers seems to be a valuable tool for molecular relationships and classification among the silkworms.

• Journal of Ginseng Research
• /
• v.40 no.2
• /
• pp.151-159
• /
• 2016

Background: Ginsenoside-Rg3, the pharmacologically active component of red ginseng, has been found to inhibit tumor growth, invasion, metastasis, and angiogenesis in various cancer models. Previously, we found that 20(R)-ginsenoside-Rg3 (Rg3) could inhibit angiogenesis. Since microRNAs (miRNAs) have been shown to affect many biological processes, they might play an important role in ginsenoside-mediated angiomodulation. Methods: In this study, we examined the underlying mechanisms of Rg3-induced angiosuppression through modulating the miRNA expression. In the miRNA-expression profiling analysis, six miRNAs and three miRNAs were found to be up- or down-regulated in vascular-endothelial-growth-factor-induced human-umbilical-vein endothelial cells (HUVECs) after Rg3 treatment, respectively. Results: A computational prediction suggested that mature hsa-miR-520h (miR-520h) targets ephrin receptor (Eph) B2 and EphB4, and hence, affecting angiogenesis. The up-regulation of miR-520h after Rg3 treatment was validated by quantitative real-time polymerase chain reaction, while the protein expressions of EphB2 and EphB4 were found to decrease, respectively. The mimics and inhibitors of miR- 520h were transfected into HUVECs and injected into zebra-fish embryos. The results showed that overexpression of miR-520h could significantly suppress the EphB2 and EphB4 protein expression, proliferation, and tubulogenesis of HUVECs, and the subintestinal-vessel formation of the zebra fish. Conclusion: These results might provide further information on the mechanism of Rg3-induced angiosuppression and the involvement of miRNAs in angiogenesis.

• BMB Reports
• /
• v.49 no.5
• /
• pp.249-254
• /
• 2016

All living things share some common life processes, such as growth and reproduction, and have the ability to respond to their environment. However, each type of organism has its own specialized way of managing biological events. Genetic sequences determine phenotypic and physiological traits. Based on genetic information, comparative genomics has been used to delineate the differences and similarities between various genomes, and significant progress has been made in understanding regenerative biology by comparing the genomes of a variety of lower animal models of regeneration, such as planaria, zebra fish, and newts. However, the genome of lizards has been relatively ignored until recently, even though lizards have been studied as an excellent amniote model of tissue regeneration. Very recently, whole genome sequences of lizards have been uncovered, and several attempts have been made to find regeneration factors based on genetic information. In this article, recent advances in comparative analysis of the lizard genome are introduced, and their biological implications and putative applications for regenerative medicine and stem cell biology are discussed.

• Journal of the Korean Society of Clothing and Textiles
• /
• v.30 no.6 s.154
• /
• pp.840-850
• /
• 2006

This thesis that is written by inquiring the shoes of 1990's, is written in order to propose the method of analyzing the changes of shoes design systematically. The picture to be gathered was a content analysis low at ARS and Accessory Collegioni since though the research method took shoes to $1990{\sim}1999$. I categorized the design factors into shape, material, color, decoration. by using those category, the result of analysis of design character. In 1,990's, boots, pumps and straps were the most popular in upper, point and rounded cuban and french were the most popular in toe, leather was the most popular in materiel, suede in F/W and patent in S/S. Black was the most popular color, brown in F/W and white/beige in S/S were the second. Re-created shape that was constructed through the reconstructions of structures and shapes of shoes, shape that was expanded to other genre were appeared in character of design factor. Material image was strengthened by varying patterns of unburned calf, leopard and zebra. Recognition of shoes color was expanded by using unique colorations and abstract, fresh figure colors. Post-modem tendency was appeared like using brassiere strap instead of back strap or using round string in elegant shoes.