• 제목/요약/키워드: Zea Mays L.

검색결과 270건 처리시간 0.039초

Variations in endopolyploidy level during the short period of the early growing stage in the roots and leaves of maize (Zea mays) seedlings

  • Ogawa, Atsushi;Taguchi, Nanako;Miyoshi, Kazumitsu
    • Plant Biotechnology Reports
    • /
    • 제4권2호
    • /
    • pp.117-123
    • /
    • 2010
  • We used a flow cytometer to investigate the variations in endopolyploidy (the frequencies of nuclei with DNA contents equivalent to 4C through 16C) during the short period of the early growing stage in vigorously growing young tissues of maize seedlings. We examined different portions of the root and leaves that had been growing for 7 (day 7) and 13 (day 13) days after germination. Endoreplication showed two opposing phenomena without aging. In one case, the endopolyploidy of the first leaf was higher on day 13 than on day 7. In the latter case, endopolyploidy decreased, as clearly revealed by a comparison of the endopolyploidy of the second leaves and the 160-170 mm portion of the seminal root on days 7 and 13. Endopolyploidy was also lower in the top of the leaf. In roots, endopolyploidy was increased by the exogenous application of abscisic acid for only 1 day. The levels of endopolyploidy increased without an increase in cell size in the roots. These results showed that endoreplication occurs in actively growing and young tissue and that the variation can be induced in the short period examined.

Total polyphenol and ferulic acid analysis of a new variety of corn, Bandiburichodang, according to steaming time and roasting temperature

  • Nari Yoon;Hak-Dong Lee;Uyoung Na;A Ram Yu;Min-Jung Bae;Gunhwa Park;Sanghyun Lee
    • Journal of Applied Biological Chemistry
    • /
    • 제66권
    • /
    • pp.305-310
    • /
    • 2023
  • Bandiburichodang (BDC) is a new variety of Zea mays L. Total polyphenol content (TPC) assay and quantitative analysis of ferulic acid (FA) were performed to determine the steaming, roasting conditions of BDC kernels that lead to the highest content. TPC levels increased after roasting under all conditions. TPC levels in samples steamed at 115 ℃ for 25 min were 3.157 mg/g before roasted, and increased to 3.825 and 4.739 mg/g after roasting at 160 and 200 ℃, respectively. Whether BDC kernels were roasted was relevant with TPC content. BDC kernels were extracted to perform quantitative analysis of FA. Roasting temperature affected FA content: the higher the temperature, the lower the content. BDC kernels that were steamed at 115 ℃ for 25 min had 0.178 mg/g of FA content before roasting, and levels decreased to 0.132 and 0.115 mg/g after roasting. Under different roasting conditions, FA content decreased 15 to 50%. We hypothesize that this phenomenon is due to a breakdown of phenolic compounds or cell wall disruption.

오염된 곡물류(밀, 콩, 옥수수)에서 주요 진균독소 검출 (Detection of Major Mycotoxins from Contaminated Cereals (Wheat, Soybean and Corn))

  • 정일민;김은영;백수봉;유승헌
    • 분석과학
    • /
    • 제12권6호
    • /
    • pp.534-539
    • /
    • 1999
  • Penicillium, Aspergillus 및 Fusarium에 오염된 밀(Triticum aestivum L.), 콩(Glycine max Merr), 옥수수(Zea mays L.)에서 HPLC를 이용하여 주요 독소들을 검정하였다. Penicillium 독소인 brefeldin A는 밀에서 3.1~270 ppm, 콩에서 45~230 ppm, 옥수수에서 1030~1240 ppm 다량 검출되었다. Citreoviridin과 griseofulvin은 밀, 콩, 옥수수에서 각각 40~80 ppm과 3.6~26.0 ppm이 검출되었다. 그리고 citrinin과 patulin은 밀, 콩, 옥수수에서 각각 0.3~4.0 ppm과 420~3800 ppm로 검출되었고, 특히 옥수수에서 다량 검출되었다. Aspergillus 독소로 ochratoxin A는 밀에서 730 ppm, 콩에서 12.4 ppm, 옥수수는 310 ppm이 검출되었다.

  • PDF

옥수수의 항산화 활성에 대한 3요소 비료의 효과 (Effects of NPK Fertilizers on Antioxidant Activity of Corn(Zea mays L.))

  • 서영호;허남기;정영상;이해익;민황기
    • 한국토양비료학회지
    • /
    • 제33권2호
    • /
    • pp.85-91
    • /
    • 2000
  • 옥수수의 항산화 활성에 대한 N-P-K의 시비의 효과를 알아보고자, 찰옥 2호와 수원 19호 두 품종을 대상으로 시비량에 따른 옥수수의 항산화 활성과 tocopherols, 페놀화합물, carotenoids의 함량을 분석하였다. 시비 수준은 N, P, K 각각에 대한 표준 시비량과 이의 1/2 및 2배 그리고 무비이었다. 찰옥2호의 항산화 활성은 19.0~22.2%로, 수원19호보다 높았다. 찰옥 2호의 항산화 활성은 표준 시비구에서 가장 높았으나, 처리간의 유의성은 없었고, 수원19호의 항산화활성과 항산화 성분 함량은 질소 2배 시용구에서 높았다. Tocopherols, 페놀화합물, carotenoids의 함량은 찰옥2호의 경우 각각 30.0~38.1, 104.7~118.8, $0.1{\mu}gml^{-1}$였고, 수원19호는 각각 16.7~20.1, 59.9~72.7, $35.5{\sim}50.0{\mu}gml^{-1}$로 항산화 활성과 마찬가지로 변이폭이 작고, 시비량에 따른 뚜렷한 경향을 보이지 않았다. ASI와 항산화 활성은 옥수수의 수량과 정상관이 있었으며, 항산화 활성은 tocopherols, 페놀화합물의 함량과 정상관이 있었으나 carotenoids와는 상관관계가 없었다. 옥수수의 항산화 활성과 항산화성분 함량은 품종간 변이의 영향이 시비량에 의한 환경변이의 영향보다 큰 것으로 보인다.

  • PDF

한우퇴비 시용에 따른 옥수수(Zea mays L.)의 생육에 미치는 영향 (Influence of Hanwoo (Korean Native Cattle) Manure Compost Application in Soil on the Growth of Maize (Zea mays L.))

  • 변지은;이준경;박민수;조나연;김수량;홍성하;이병오;이명규;황선구
    • 한국작물학회지
    • /
    • 제67권3호
    • /
    • pp.164-171
    • /
    • 2022
  • 화학비료 처리구가 한우퇴비 처리구보다 출웅기는 2일, 출사기는 4일정도 빨랐다. 초장은 생육초기에는 화학비료 처리구가 가장 길었으나 생육후기(정식 55일차)에는 한우퇴비 처리구가 화학비료 처리구보다 초장이 길었다. 이는 화학비료 처리구가 한우퇴비 처리구보다 출사기가 빨라 화학비료 처리구의 생장기간이 한우퇴비 처리구보다 짧았기 때문에 나타난 현상으로 보인다. 수확 후 이삭길이는 한우퇴비 시용 처리구에서 가장 길었으며, 이삭 직경, 이삭무게, 백립중의 경우에는 화학비료 처리구와 한우퇴비 시용 처리구가 유의한 차이를 보이지 않았다. 종합적으로 한우퇴비 시용 시 이삭길이의 경우에는 한우퇴비 시용하는 것이 더 길었으며, 이삭무게와 백립중은 화학비료 시용 시와 큰 차이를 보이지 않아 한우퇴비가 화학비료를 대체할 수 있을 것으로 보인다.

Identification and molecular characterization of downy mildew resistant gene candidates in maize (Zea mays subsp. Mays)

  • Kim, Jae Yoon;Kim, Chang-Ho;Kim, Kyung Hee;Lee, Byung-Moo
    • 한국작물학회:학술대회논문집
    • /
    • 한국작물학회 2017년도 9th Asian Crop Science Association conference
    • /
    • pp.113-113
    • /
    • 2017
  • Downy mildew (DM), caused by several species in the Peronosclerospora and Scleropthora genera, is a major maize (Zea mays L.) disease in tropical or subtropical regions. DM is an obligate parasite species in the higher plants and spreads by oospores, wind, and mycelium in seed surface, soil, and living hosts. Owing to its geographical distribution and destructive yield reduction, DM is one of the most severe maize diseases among the maize pathogens. Positional cloning in combination with phenotyping is a general approach to identify disease resistant gene candidates in plants; however, it requires several time-consuming steps including population or fine mapping. Therefore, in the present study, we suggest a new combination strategy to improve the identification of disease resistant gene candidates. Downy mildew (DM) resistant maize was selected from five cultivars using the spreader row technique. Positional cloning and bioinformatics tools identified the DM resistant QTL marker (bnlg1702) and 47 protein coding genes annotations. Eventually, 5 DM resistant gene candidates, including bZIP34, Bak1, and Ppr, were identified by quantitative RT-PCR without fine mapping of the bnlg1702 locus. Specifically, we provided DM resistant gene candidates with our new strategy, including field selection by the spreader row technique without population preparation, the DM resistance region identification by positional cloning using bioinformatics tools, and expression level profiling by quantitative RT-PCR without fine mapping. As whole genome information is available for other crops, we propose applying our novel protocol to other crops or for other diseases with suitable adjustment.

  • PDF