• BMB Reports
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• 제51권8호
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• pp.388-393
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• 2018

The activating transcription factor (ATF) 4 belongs to the ATF/CREB (cAMP Response Element Binding bZIP [Basic Leucine Zipper]) transcription factor family, and plays a central role in the UPR (Unfolded Protein Response) process in cells. The induction of ATF4 expression has previously been shown to increase the replication of HIV-1. However, the detailed mechanism underlying this effect and the factors involved in the regulation of ATF4 function are still unknown. Here, we demonstrate first that knocking out ATF4 using siRNA shows a strong negative effect on HIV-1 production, indicating that ATF4 is a functional positive cellular factor in HIV-1 production. To determine the mechanism by which ATF4 regulates the HIV-1 life cycle, we assessed the effect of the overexpression of wild type ATF4 and its various derivatives on HIV-1 LTR-mediated transcriptional activation and the production of HIV-1 particles. This effect was studied through co-transfection experiments with either reporter vectors or proviral DNA. We found that the N-terminal domains of ATF4 are involved in HIV-1 LTR-mediated transcriptional activation, and thus in HIV-1 production.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제10권6호
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• pp.2851-2873
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• 2016

Cloud storage as a service provides high scalability and availability as per need of user, without large investment on infrastructure. However, data security risks, such as confidentiality, privacy, and integrity of the outsourced data are associated with the cloud-computing model. Over the year's techniques such as, remote data checking (RDC), data integrity protection (DIP), provable data possession (PDP), proof of storage (POS), and proof of retrievability (POR) have been devised to frequently and securely check the integrity of outsourced data. In this paper, we improve the efficiency of PDP scheme, in terms of computation, storage, and communication cost for large data archives. By utilizing the capabilities of JAR and ZIP technology, the cost of searching the metadata in proof generation process is reduced from O(n) to O(1). Moreover, due to direct access to metadata, disk I/O cost is reduced and resulting in 50 to 60 time faster proof generation for large datasets. Furthermore, our proposed scheme achieved 50% reduction in storage size of data and respective metadata that result in providing storage and communication efficiency.

• 한국기록관리학회지
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• 제16권4호
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• pp.79-120
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• 2016

변화하는 컴퓨팅 환경에 따라 공문서 생산 환경이 G-클라우드 및 클라우드 온-나라 시스템으로 전환하고 있다. 더불어 공문서 포맷도 독자 포맷인 HWP을 대신하여 개방형 규격인 ODF가 정착할 것으로 전망된다. 이에 본 연구는 현행 공문서 장기보존 전략을 재검토 하고, ODF 생산 문서 보존에 적합한 문서보존포맷과 장기보존포맷 설계 방향을 제시하고자 한다. 생산 포맷이 ODF인 기록은 문서보존포맷으로의 변환 없이 기록관리 메타데이터를 추가한 확장 ODF 포맷을 제안하고자 하며 독자포맷의 보존에도 ODF를 검토하였다. 장기보존 포맷, 즉 NEO 포맷 변환 현황을 알아보고 BagIt 규격 패키지를 ISO21320으로 제정된 ZIP을 적용하여 장기보존포맷을 설계하였다.