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http://dx.doi.org/10.5483/BMBRep.2018.51.8.054

Understanding of the functional role(s) of the Activating Transcription Factor 4(ATF4) in HIV regulation and production  

Lee, Seong-Deok (National Research Laboratory for Molecular Virology, Department of Pathology, School of Medicine, The Catholic University of Korea)
Yu, Kyung-Lee (National Research Laboratory for Molecular Virology, Department of Pathology, School of Medicine, The Catholic University of Korea)
Park, Seong-Hyun (National Research Laboratory for Molecular Virology, Department of Pathology, School of Medicine, The Catholic University of Korea)
Jung, Yu-Mi (National Research Laboratory for Molecular Virology, Department of Pathology, School of Medicine, The Catholic University of Korea)
Kim, Min-Jeong (Avixgen Inc.)
You, Ji-Chang (National Research Laboratory for Molecular Virology, Department of Pathology, School of Medicine, The Catholic University of Korea)
Publication Information
BMB Reports / v.51, no.8, 2018 , pp. 388-393 More about this Journal
Abstract
The activating transcription factor (ATF) 4 belongs to the ATF/CREB (cAMP Response Element Binding bZIP [Basic Leucine Zipper]) transcription factor family, and plays a central role in the UPR (Unfolded Protein Response) process in cells. The induction of ATF4 expression has previously been shown to increase the replication of HIV-1. However, the detailed mechanism underlying this effect and the factors involved in the regulation of ATF4 function are still unknown. Here, we demonstrate first that knocking out ATF4 using siRNA shows a strong negative effect on HIV-1 production, indicating that ATF4 is a functional positive cellular factor in HIV-1 production. To determine the mechanism by which ATF4 regulates the HIV-1 life cycle, we assessed the effect of the overexpression of wild type ATF4 and its various derivatives on HIV-1 LTR-mediated transcriptional activation and the production of HIV-1 particles. This effect was studied through co-transfection experiments with either reporter vectors or proviral DNA. We found that the N-terminal domains of ATF4 are involved in HIV-1 LTR-mediated transcriptional activation, and thus in HIV-1 production.
Keywords
Activating Transctiption Factor 4; ATF4; HIV-1; HIV-1 LTR; HIV-1 promoter; Transctiption activation;
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Times Cited By KSCI : 2  (Citation Analysis)
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