• 한국균학회소식:학술대회논문집
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• 2016.05a
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• pp.27-27
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• 2016

The ascomycete fungus Fusarium graminearum is the most common pathogen of Fusarium head blight (FHB), a devastating disease for major cereal crops worldwide. FHB causes significant crop losses by reducing grain yield and quality as well as contaminating cereals with trichothecenes and zearalenone (ZEA) that pose a serious threat to animal health and food safety. ZEA is a causative agent of hyperestrogenic syndrome in mammals and can result in reproductive disorders in farm animals. In F. graminearum, the ZEA biosynthetic cluster is composed of four genes, PKS4, PKS13, ZEB1, and ZEB2, which encode a reducing polyketide synthase, a nonreducing polyketide synthase, an isoamyl alcohol oxidase, and a transcription factor, respectively. Although it is known that ZEB2 primarily acts as a regulator of ZEA biosynthetic cluster genes, the mechanism underlying this regulation remains undetermined. In this study, two isoforms (ZEB2L and ZEB2S) from the ZEB2 gene in F. graminearum were characterized. It was revealed that ZEB2L contains a basic leucine zipper (bZIP) DNA-binding domain at the N-terminus, whereas ZEB2S is an N-terminally truncated form of ZEB2L that lacks the bZIP domain. Interestingly, ZEA triggered the induction of both ZEB2L and ZEB2S transcription. In ZEA producing condition, the expression of ZEB2S transcripts via alternative promoter usage was directly or indirectly initiated by ZEA. Physical interaction between ZEB2L and ZEB2L as well as between ZEB2L and ZEB2S was observed in the nucleus. The ZEB2S-ZEB2S interaction was detected in both the cytosol and the nucleus. ZEB2L-ZEB2L oligomers activated ZEA biosynthetic cluster genes, including ZEB2L. ZEB2S inhibited ZEB2L transcription by forming ZEB2L-ZEB2S heterodimers, which reduced the DNA-binding activity of ZEB2L. This study provides insight into the autoregulation of ZEB2 expression by alternative promoter usage and a feedback loop during ZEA production.

• Biomedical Science Letters
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• v.28 no.1
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• pp.9-24
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• 2022

The aim of this study was to identify the expression of zinc finger E-box binding homeobox 1 (ZEB1), its prognostic significance, and correlation between ZEB1 and infiltrating immune cells in lung cancer. Correlation between ZEB1 and telomerase was also analyzed in different types of cancers. RNA sequencing analysis and survival rates of patients were confirmed by Gene Expression Profiling Interactive Analysis (GEPIA). The Kaplan-Meier plotter and PrognoScan databases were used to analyze the prognostic value of ZEB1 in various cancers. The Tumor IMmune Estimation Resource (TIMER) was used to determine the correlation between ZEB1 and infiltrating immune cells. Lower ZEB1 expression was lower in lung cancer and was related to poor prognosis in lung adenocarcinoma (LUAD). ZEB1 expression exhibited a significantly positive correlation with infiltration levels of immune cells in LUAD and lung squamous cell carcinoma. Furthermore, we found that the ZEB1 expression correlated with subunits of telomerase. Our findings suggest ZEB1 as a potential biomarker to be used for prognostic significance and tumor immunology in lung cancer. The correlation between the expression of ZEB1 and telomere-related gene will help in understand the cancer-promoting mechanisms.

• Journal of Digital Convergence
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• v.20 no.2
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• pp.277-287
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• 2022

ZEB is a building that increases the energy independence of the building itself, and new and renewable elements that can produce energy are essential, and BIPV is the most notable technology. In ZEB's design, BIPV should be planned early in the design, but BIPV plans are insufficient in the early stages. Therefore, this study derived elements for theoretical consideration of BIM and ZEB and analysis of ZEB independence rate based on BIM, a convergence design technology, and analyzed BIPV energy production and building energy consumption. Finally, by calculating the energy independence rate and reviewing the rating criteria in the project model, a basic research method for calculating the energy independence rate of ZEB at the beginning of the design was presented. Through this, it is expected that design productivity can be improved by supporting the decision of ZEB subjects.

• Journal of Microbiology and Biotechnology
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• v.26 no.5
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• pp.967-974
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• 2016

Zearalenone (ZEA) is an estrogenic mycotoxin that is produced by several Fusarium species, including Fusarium graminearum. One of the ZEA biosynthetic genes, ZEB2, encodes two isoforms of Zeb2 by alternative transcription, forming an activator (Zeb2L-Zeb2L homooligomer) and an inhibitor (Zeb2L-Zeb2S heterodimer) that directly regulate the ZEA biosynthetic genes in F. graminearum. Cyclic AMP-dependent protein kinase A (PKA) signaling regulates secondary metabolic processes in several filamentous fungi. In this study, we investigated the effects of the PKA signaling pathway on ZEA biosynthesis. Through functional analyses of PKA catalytic and regulatory subunits (CPKs and PKR), we found that the PKA pathway negatively regulates ZEA production. Genetic and biochemical evidence further demonstrated that the PKA pathway specifically represses ZEB2L transcription and also takes part in posttranscriptional regulation of ZEB2L during ZEA production. Our findings reveal the intriguing mechanism that the PKA pathway regulates secondary metabolite production by reprograming alternative transcription.

• Proceedings of the KIEE Conference
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• 2009.07a
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• pp.2186_2187
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• 2009

본 논문은 Zero energy building(ZEB) 또는 net zero energy building의 정의와 개념에 대해서 서술 하였다, 그리고 ZEB의 장단점을 알아보고 연구 방향에 대해서 논하였다. ZEB는 주거와 상업적 빌딩에 신재생에너지를 이용하고 에너지 소비 효울을 높여 큰 에너지 절감을 통해 경제적 이익을 얻을 수 있고 저탄소 배출로 환경을 보존 할 수 있는 녹색성장의 한 방법이다. "Zero energy"라는 단어는 요즘 많이 사용 되는데 비해 정확한 정의와 개념에 대해서는 이해가 부족하다. 따라서 본 논문에서는 ZEB에 대해 정확한 정의와 세계적 흐름, ZEB의 장단점 그리고 앞으로 연구해야 할 방향에 대해 논의 하겠다.

• Journal of Korean Institute of Architectural Sustainable Environment and Building Systems
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• v.12 no.6
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• pp.557-566
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• 2018

The ZEB certification system has come into effect since 2018 according to the ZEB road map. From 2020, the public buildings with the total floor area smaller than $3000m^2$ are the target of the certification, and it will be extended to the buildings with up to $5000m^2$ floor area in 2025. However, current mandatory regulations for public office buildings seem already to meet the ZEB certification system planned for 2025. In this work, two buildings belong to $3,000{\sim}5,000m^2$ in total floor area were selected to analyze the possibility of meeting the ZEB certification only by following current obligation regulations. Results showed that the test buildings satisfied the minimum building energy efficiency and energy self-sufficiency rates for the ZEB certification when the mandatory insulation and installation ratio of renewable energy are applied. This can be useful for revising the road map or extending the target buildings of the ZEB certification.

• Journal of the Architectural Institute of Korea Structure & Construction
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• v.36 no.3
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• pp.147-157
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• 2020

With the increasing obligation to reduce greenhouse gas emissions to the building sector, the government has been gradually expanding its obligation to zero-energy buildings since 2020. Since the ZEB certification took effect in 2017, 48 preliminary and main certifications have been completed as of March 2019, and most of them are public buildings or even certified, but have earned Grade 5 of ZEB. This means that compared with the number of annual building permits registered in Korea, the figure is insignificant, and that it receives little if not mandatory. Therefore, this study investigated empirical cases of ZEB additional construction cost and conducted cost benefit analysis according to changes in ZEB additional construction cost based on a preliminary feasibility analysis project recently conducted by the KDI. In addition, considering the public buildings, the social costs were considered, and the cost-benefit analysis method was the same as the KDI's preliminary feasibility analysis method. The analysis shows that if the ratio of ZEB additional construction cost is more than 5 percent, it is analyzed that there is no economic feasibility, and considering that the ratio of additional construction cost in the cases of ZEB in Korea is 17 percent to 38 percent, it will not be easy to obtain ZEB certification in terms of cost. Finally, to narrow these differences in cost and economic aspects, the overseas low energy and ZEB incentive examples proposed measures such as the concept of subsidy payment in Illinois and the compensation of social costs to private ZEB.

• Journal of the Korean Society for Geothermal and Hydrothermal Energy
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• v.19 no.1
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• pp.1-13
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• 2023

Many government in the world have conducted building energy performance certification program to reduce building energy consumption. In this study, a reference building and its HVAC system was modeled, and the energy load and consumption were estimated by the ECO2 program. The software is a simple building energy simulation program based on monthly calculated method. The building energy efficiency rating the the reference building was 1+ under baseline condition. The simulation results showed that the insulation performance slightly affected building energy load and consumption, but light density had a significant effect on them. The application of geothermal heat pumps gave improvement of building energy efficiency rating but it could not make it possible to get zero energy building(ZEB) certification. The ZEB 5 certification could be achieved by using photovoltaics, however getting better grade was difficult. The simulation results showed that the ZEB 4 certification, one grade higher than ZEB 5, could be attained by using more than one renewable energy source such as geothermal and solar energy in this study.

• Journal of the Korean Solar Energy Society
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• v.39 no.3
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• pp.1-7
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• 2019

As part of the government's energy policy, Zero Energy Building certification was launched on January of 2017. However, the three passive-housing rental housing projects are the only ZEB-certified detached housing since the certification's launch. The reason is that, in order for a detached housing to earn ZEB certification, it has to secure self-reliance in energy, and a photovoltaic system is the only viable renewable energy system. Therefore, conducting an analysis to optimize the photovoltaic system in an early design stage is strongly recommended. This study aimed to propose an optimal photovoltaic system design for a detached housing after analyzing through the ECO2 energy simulation of 44 cases, varying in a module type and efficiency, inclination and azimuth. As a result, 15 cases out of 44 cases were analyzed to satisfy ZEB evaluation criteria, and it is thought that these data could contribute greatly to the expansion of ZEB certification dissemination.

• KIEAE Journal
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• v.15 no.5
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• pp.67-74
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• 2015

This study aimed to develop a design manual to be used during the initial stage of the nearly Zero Energy Building (nZEB) design process. Recently, with the increased demand for nZEBs, there are many architects and architectural firms who are becoming interested in nZEB design. However, since the nZEB design process requires a different approach to the conventional building design process, architects have difficulties with application of the nZEB design process in their projects. Therefore, a design manual which can be used in the nZEB design process was developed in this study. Based on an intensive literature review, energy-saving strategies and their performance levels, which affect heating and cooling energy consumptions were established for a reference building. To analyze the sensitivity of each energy strategy to the overall performance, computer simulations using EnergyPlus were performed. At the same time, an Analysis of Variance assessment was conducted to estimate the relative importance of each energy factor. The energy sensitivity and priority of the energy factors was developed into a set of design guidelines.