• Toxicological Research
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• 제20권3호
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• pp.251-261
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• 2004

A large number of chemical pollutants including phthalates, alkylphenolic compounds and organochlorine pesticides have the ability to disrupt endocrine function in animals, and alter cog-nitive function. Because hormone mediated events play an important role in central nervous system development and function, the changes in cognitive function seem to be mediated by the endocrine-like action of these chemicals. The present study therefore was designed to investigate effect of bisphenol A (BPA), an endocrine disrupting chemical on neuro-behavial patterns, and expression of estrogen receptors and tyrosine hydroxylase, a limiting enzyme of dopamine synthesis pathway. BPA was treated orally for 3 weeks into 3 week old mice, and then the neuro-behavial patterns (stereo-type behaviors such as jumping rearing and forepaw tremor, climbing behavior, tail flick, rotarod and locomotor activity), and the expression of estrogen receptors and tyrosine hydroxylase were deter-mined every 3 week for 9 weeks. During the treatment of BPA, the food uptake and body weight increase were not significantly changed. BPA resulted in the increased stereotype behaviors (jump-ing, rearing and forepaw tremor) 6 or 9 weeks after treatment. The time response to tail flick and locomotor activity were decreased by the treatment of BPA, whereas the time for rotarod was increased by the treatment of BPA. The expression of estrogen receptor alpha and beta was increased in the brain and pituitary gland. Maximum expression was found in the brain after 9 week of 100 mg/kg BPA treatment and in the pituitary gland after 6 week of 100 mg/kg BPA treatment. Tyrosine hydroxylase was increased in dose and time dependent manners in the brain but no change was found in the pituitary gland. The present data show that exposure of BPA in the young mice could alter expression of estrogen receptors and dopamine synthesis pathway, thereby modulate neuro-behavial patterns (increase of stereotype behaviors but decrease locomotor activity).

• 한국산림과학회지
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• 제99권5호
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• pp.698-705
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• 2010

The concentration of monoterpenes (12 species) was measured from spring to fall in 2008 in ambient air at six different forests located in the southern parts of Korea. Sampling - using a Solid Adsorption Tube - was conducted at 2-hour-interval a day (24 hours) of each season. The highest annual average concentration of total monoterpene was 0.715 ppbv, which occurred at Mt. Baega. ${\alpha}$-Pinene, sabinene and ${\beta}$-pinene were the most abundant compounds throughout the sampling periods and areas. The concentrations of monoterpene were higher in spring than in any other seasons. The compositions and concentrations of monoterpene displayed a distinct pattern by area. Most of monoterpenes had diurnal variations with higher concentrations during the daytime, and lower during the nighttime. This may be due to the fact that the amount of monoterpenes emitted increases at elevated ambient temperatures and increased sunlight intensity. The results of this study showed that each area had distinct monoterpene compositions, which depended on the tree species, topography and local climate at each area.

• Journal of Applied Biological Chemistry
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• 제44권3호
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• pp.113-118
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• 2001

Xylose (glucose) isomerase was purified to homogeneity from cell-extracts of Streptomyces chibaensis J-59 via ammonium sulfate precipitation followed by chromatography on DEAE-cellulose, and gel filtration on Sephacryl S-300. The purified enzyme is a homotetramer with a native molecular mass of 180 kDa and a subunit molecular mass of 44 kDa. The amino acid N-terminal sequence of glucose isomerase from S. chibaensis J-59 was determined to be Ser-Tyr-Gln-Pro-Thr-Pro-Glu-Asp-Arg-Phe-Thr-Phe-Gly-Leu. The first 14 amino acids of the N-terminal sequence of the enzyme showed strong analogies with N-terminal sequences of glucose isomerase produced by other Streptomyces spp. The optimum pH and temperature for activity were 7.5 and 85, respectively. The purified enzyme required $Mg^{2+}$, $Co^{2+}$, and $Mn^{2+}$ for the activity, $Mg^{2+}$ being the most effective. The enzyme was not inhibited by $Ca^{2+}$, but was inhibited by $Hg^{2+}$, $Ag^+$, and $Cu^{2+}$. The $K_m$, $V_{max}$, and $k_{cat}$ values of S. chibaensis J-59 isomerase for glucose were 83 mM, 40.9 U/mg, and $1,843min^{-1}$, respectively. In the presence of $Co^{2+}$, cell-free enzymes retained 100% without loss of activities by the heat-treatment at $70^{\circ}C$ for 7 days. The enzyme retained 50% residual activity after heating at $85^{\circ}C$ for 13.5 h, at $90^{\circ}C$ for 126 min. The enzyme is more thermostable than any other glucose isomerases of Streptomyces spp.

• 한국환경복원기술학회지
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• 제19권4호
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• pp.45-59
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• 2016

This study introduced wood waste into revegetation base materials for recovery of highway cut slope. The object of this study is to derive the mixing ratio of wood waste, kaoline and silica by conducting a test installation, monitoring, analysis and evaluation. The following items were investigated and analyzed each experimental zone to deduce the mixing ratio of wood waste, kaoline and silica on slope revegetation base materials: the physical and chemical properties of the vegetation base materials, soil-hardness, soil-humidity, collapse and eroded spots, established number of trees, existing species of grass and tree species, vegetation coverage ratio, pest status, and invasion of disturbance species. The revegetation method was evaluated in each experiment zone which had different mixing ratios. As a result, experiment zone C scored 47 points out of 60 as the best revegetation method. However, this result has been derived from just one construction and short-term monitoring. In order to derive the suitable and dependable mixing ratio, conducting an objective revegetation method evaluation and long-term experimenting and monitoring is required.

• 대한기계학회논문집A
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• 제39권5호
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• pp.507-515
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• 2015

본 연구는 5.5MW 증속기용 기계식 동력순환 시험 장비의 핵심 기능을 하는 토크 인가 장치의 개발 및 검증에 관한 것으로, 설계 및 해석이 수행되었다. 또한, 시험 장비의 각 부품에 대하여 회전각을 측정하여 비틀림 강성을 확인하였다. 시험체를 제외한 시험 장비에 토크를 인가하여 각 부품의 회전각을 측정한 결과 시험 장비의 비틀림 강성은 정방향에서 231.13 kNm/rad으로 나타났으며, 증속기 1대의 비틀림 강성이 1,064,400 kNm/rad보다 큰 경우 정격 토크를 인가할 수 있다. 따라서 시험 장비의 회전각 한계로 인하여 시험체 증속기의 비틀림 강성에 따라 정격 토크 인가 여부가 결정된다.

• 대한수의학회지
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• 제35권4호
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• pp.671-681
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• 1995

This study was attempted to investigate the topographical distribution, shape and immunoreactivity of growth hormone-releasing factor(GRF)- and somatostatin(SOM)-immunoreactive neurons in the hypothalamus of the Korean squirrels(Sciurus vulgalis coreae). For the light microscopical examination of immunohistochemistry, the brains were fixed with 4% paraformaldehyde solution by means of intracardiac perfusion. And the frozen sections($40{\mu}m$ thick) were stained immunohistochemically by ABC method. Distribution of GRF immunoreactive neurons($12-17{\mu}m$) was highest in the paraventricular nucleus, moderate in the periventricular and supraoptic nuclei, and low in the arcuate nucleus and lateral hypothalamic area. Their immunoreactive fibers were found very high in the median eminence, moderately in the supraoptic, paraventricular and periventricular nuclei, and low in the arcuate nucleus and lateral hypothalamic area. SOM immunoreactive perikarya($14-18{\mu}m$) were found moderately in the periventricular nucleus near the subependymal layer of the third ventricle, and low in the arcuate and suprachiasmatic nuclei. SOM immunoreactive fibers were found high in the median eminence, and moderately or low in the arcuate and periventricular nuclei.

• Spatial Information Research
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• 제17권2호
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• pp.231-241
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• 2009

현재 21세기 지식정보화사회와 유비쿼터스국토의 기반을 조성하는 3차원 국토공간정보의 구축을 위한 품질 관리의 중요성이 강조되고 있다. 통합품질관리를 통한 3차원 국토공간정보 품질관리의 향상은 공급자가 효과적으로 데이터를 갱신하고, 사용자가 최상의 자료를 취득할 수 있게 해준다. 본 연구는 기존 3차원 품질관리와 사례분석를 통하여 3차원 국토공간정보 품질관리개선안을 마련하고자 하였다. 본 연구에서는 첫째, 3차원 국토공간정보 품질관리의 개념을 수립하였고 둘째, 3차원 국토공간정보 품질관리의 범위와 내용 및 절차는 구체적인 세부 항목으로 분류하여 설정하였으며, 마지막으로 구축지침 개선안에 따라 3차원 국토공간정보의 구축 공정과정별로 품질관리표 등 품질과 관련된 개선사항을 도출하였다.

• 임상간호연구
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• 제22권1호
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• pp.20-27
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• 2016

Purpose: The purpose of this study was to compare the fluid intake and output (I&O) measurement methods in order to figure out more effective and easier method for medical patients Methods: 71 hospitalized patients participated in the study. In "liquid only (LO)" method, all amount of water was summed up including any liquid types of food and IV fluids. In "whole food(WF) intake," all liquid and solid food intake and IV fluids were added up. Results: The average amount of fluid intake was 2105.29 ml for LO method and 2523.54 ml for WF method. The average amount of fluid output was 2148.98 ml. The intra-class correlations (ICC) between the intake and output measures by the two different methods was 0.803 and 0.826, respectively. The correlation between the differences of intake/output and body weight change in two different methods was r=.347 (p=.003), and r=.376 (p=.001), respectively. Conclusion: The results of this study indicate that both LO and WF method may be useful in monitoring patients' fluid balance. Given the comparability of using LO over WF, it is suggested that measuring just liquid only intake as the indicator of patient's intake is applicable in clinical setting.

• 대한수의학회지
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• 제39권5호
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• pp.896-907
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• 1999

The morphometrical characteristics such as external measurements and mandible size assessment in mice and rats have to be highly heritable and sufficiently variable between strains in order to calculate a strain specific profiles. The coat color of Korean wild rats were observed and morphometric analysis of external measurements were carried out on Korean wild rats compared to laboratory strains in order to clarify the genetic characteristics of Korean wild rats and to establish background data as a domestication of Korean wild rats for new laboratory strain. Korean wild rats were captured from Chunchon and Hoengsong. 4 inbred and 1 outbred strains of rats were used in this study for the comparison of genetic characteristic of Korean wild rats. Total body length, head length, tail length, hind foot length and ear length were measured and then statistical analysis were carried out by discrimiant analysis. The coat color of Korean wild rat showed golden white in ventral portion and dark agouti in dorsal portion. Korean wild rats could be distinguished from the other laboratory strains distinctly by morphogenetical analysis. There was significant variations among Korean wild rat compared to those of the other laboratory strains of rat. This study may provide that Korean wild rats have a unique genetic characterization compared to those of other inbred strains of rats based on morphogenetical characteristics by external measurements.

• 한국식품과학회지
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• 제35권6호
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• pp.1244-1247
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• 2003

발효 및 유산균 제품에 모유만을 먹은 생후 3개월된 유아의 분변으로부터 bromocresol pule을 함유한 MRS 배지에서 노란색의 집락을 형성하는 균주들을 내산성 및 담즙산 내성이 우수한 13종의 Lactobacillus 균주들을 분리하였으며 당발효성 및 생화학적 실험을 토대로 분리된 유산균들을 동정하였다. 최종적으로 선발한 균주를 L. acidophilus로 KY1909로 명명하였다. Lactic acid 생성 능력을 평가한 후 이성질체를 분석한 결과 상업적으로 이용되는 L. acidophilus NCFM, L. acidophilus CH5, L. acidophilus NOCKS 균주들은 전체 lactic acid 중에서 L(+) form이 차지하는 비율이 각각 52.49, 40.63 및 63.13%로 나타났으나, L. acidophilus KY1909는 L(+) lactic acid의 비율이 93.96%로 나타났다.