• Journal of Animal Science and Technology
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• 제54권6호
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• pp.383-394
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• 2012

Microarray analyses provide information that can be used to enhance the efficiency of livestock production. For example, microarray profiling can potentially identify the biological processes responsible for the phenotypic characteristics of porcine liver. We performed transcriptome profiling to identify differentially expressed genes (DEGs) in liver of pigs from two breeds, the Korean native pigs (KNP) and Yorkshire pigs. We correctly identified expected DEGs using factor analysis for robust microarray summarization (FARMS) and robust multi-array average (RMA) strategies. We identified 366 DEGs in liver (p<0.05, fold-change>2). We also performed functional analyses, including gene ontology and molecular network analyses. In addition, we identified the regulatory relationship between DEGs and their transcription factors using in silico and qRT-PCR analysis. Our findings suggest that DEGs and their transcription factors may have a potential role in adipogenesis and/or lipid deposition in liver tissues of two pig breeds.

• Asian-Australasian Journal of Animal Sciences
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• 제15권9호
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• pp.1232-1236
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• 2002

Direct and service sire genetic parameters for total number of pigs born (TNB), number of pigs born alive (NBA), total pig weight at birth (TWB), and average pig birth weight (ABW) were estimated by DF-REML under a multiple trait animal model. Data on 3,078 litters of the Yorkshire from Jan, 1975 to Dec, 1998 at National Livestock Research Institute were obtained. The animal model included fixed contemporary group effects and random additive direct, service sire, and residual effects. Additive genetic relationships among animals were included. A separate relationship matrix for service sires and their sire was also included. Additive direct heritability estimates for TNB, NBA, TWB, and ABW were 0.19, 0.18, 0.25 and 0.39, respectively. Service sire heritability estimates for TNB, NBA, TWB, and ABW were 0.02, 0.01, 0.02 and 0.01, respectively. The genetic and phenotypic correlations of TNB with NBA estimated in this study were 0.81 and 0.81, respectively, and the genetic and phenotypic correlations of TNB with TWB estimated were 0.82 and 0.76, respectively. Results indicate that service sires account for 1 to 2% of the total variation for TNB, NBA, TWB, and ABW. Further investigation is needed to determine whether the service sire effect is primarily genetic or environmental.

• Asian-Australasian Journal of Animal Sciences
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• 제18권5호
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• pp.620-625
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• 2005

Correlation of microsatellite heterozygosity with performance or heterosis was reported in wild animal populations and domestic animal populations, but the correlation with heterosis in a crossbreeding F$_1$ pig population remained uncertain. To explore this, we had random selected and mated Yorkshire${\times}$Meishan (F, n = 82) and their reciprocal (G, n = 47) to F$_1$, and used the two straightbreds as control groups (Yorkshire = 34, Meishan = 55), and observed the heterosis of birth weight (BWT), average daily gain (ADG) and feed and meat ratio (FMR). Two Kinds of measurement-individual heterozygosity (IH) and individual mean d$^2$ (lg value, ID) were used as index of heterozygosity and variance from 39 microsatellite marker loci to perform univariate regression analysis against heterosis. We detected significant correlation of IH with BWT in all of F$_1$ (F+G) and in F. We observed significant correlation of ID with ADG in all of F$_1$ (F+G), and with FMR in all of F$_1$ (F+G) and in F. There was significant maternal effect on heterosis, which was indicated by significant difference of means and distribution of heterosis between F and G. This difference was consistent with distributions of IH and ID, and with difference of means in F and G. From this study, it would be suggested that the two kinds of genetic index could be used to explore the genetic basis of heterosis in crossbreeding populations but could not determine which is better.

• 한국산학기술학회논문지
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• 제20권10호
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• pp.195-202
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• 2019

본 연구는 외화 낭비를 줄이기 위해 우리흑돈이 종료종모돈으로써 두록을 대체할 수 있는지 조사하고자 하였다. 본 논문에서는 랜드레이스 ${\times}$ 요크셔 ${\times}$ 두록(LYD)과 랜드레이스 ${\times}$ 요크셔 ${\times}$ 우리흑돈(LYW)의 두 삼원교잡종에서 등심의 육질 특성을 비교하기 위해 이화학적 분석을 실시하였다. 공시 동물은 180일령의 LYD 59두와 LYW 60두, 총 119두를 이용하였으며, 도축 후 24시간 동안 냉각시키고, 돈육에 대한 이화학적 분석, 육색, 지방산 조성 및 관능평가를 실시하였다. 일반 성분 결과 수분 및 보수력은 두 품종간의 유의한 차이를 나타내지 않았다. 지방 함량 및 가열감량은 LYW에서 LYD에 비해 유의적으로 높게 나타났고(p<0.05), 전단력 및 pH는 LYW가 LYD보다 유의적으로 낮게 나타났다(p<0.05). 육색지수를 확인한 결과 적색도($a^*$) 및 황색도($b^*$)가 LYW에서 유의적으로 높게 나타났다(p<0.05). 두 품종의 지방산 함량을 비교한 결과 Stearic acid(C18:0)가 LYD에 비해 LYW에서 유의적으로 높게 나타났다(p<0.05). 관능검사 결과 LYW가 LYD에 비해 육색과 풍미가 유의적으로 높게 나타났다(p<0.05). 이러한 결과는 LYD와 비교하여 LYW에서 특정 화학적 특징이 개선되었음을 나타낸다. 따라서 우리흑돈이 수입 종돈인 두록의 대체 종돈으로 사용 가능할 것으로 사료된다.

• Asian-Australasian Journal of Animal Sciences
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• 제14권7호
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• pp.910-914
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• 2001

The teat number of a sow plays an important role for weaning pigs and has been utilized in selection of swine breeding stock. Various linear models have been employed for genetic analyses of teat number although the teat number can be considered as a count trait. Theoretically, Poisson error mixed models are more appropriate for count traits than Normal error mixed models. In this study, the two models were compared by analyzing data simulated with Poisson error. Considering the mean square errors and correlation coefficients between observed and fitted values, the Poisson generalized linear mixed model (PGLMM) fit the data better than the Normal error mixed model. Also these two models were applied to analyzing teat numbers in four breeds of swine (Landrace, Yorkshire, crossbred of Landrace and Yorkshire, crossbred of Landrace, Yorkshire, and Chinese indigenous Min pig) collected in China. However, when analyzed with the field data, the Normal error mixed model, on the contrary, fit better for all the breeds than the PGLMM. The results from both simulated and field data indicate that teat numbers of swine might not have variance equal to mean and thus not have a Poisson distribution.

• Asian-Australasian Journal of Animal Sciences
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• 제14권8호
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• pp.1051-1056
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• 2001

Data for teat number for Landrace (L), Yorkshire (Y), crossbred of Landrace and Yorkshire (LY), and crossbred of Landrace, Yorkshire and Chinese indigenous Min Pig (LYM) were analyzed using Gibbs sampling. In Bayesian inference, flat priors and some informative priors were used to examine their influence on posterior estimates. The posterior mean estimates of heritabilities with flat priors were $0.661{\pm}0.035$ for L, $0.540{\pm}0.072$ for Y, $0.789{\pm}0.074$ for LY, and $0.577{\pm}0.058$ for LYM, and they did not differ (p>0.05) from their corresponding estimates of REML. When inverse Gamma densities for variance components were used as priors with the shape parameter of 4, the posterior estimates were still corresponding (p>0.05) to REML estimates and mean estimates using Gibbs sampling with flat priors. However, when the inverse Gamma densities with the shape parameter of 10 were utilized, some posterior estimates differed (p<0.10) from REML estimates and/or from other Gibbs mean estimates. The use of moderate degree of belief was influential to the posterior estimates, especially for Y and for LY where data sizes were small. When the data size is small, REML estimates of variance components have unknown distributions. On the other hand, Bayesian approach gives exact posterior densities of variance components. However, when the data size is small and prior knowledge is lacked, researchers should be careful with even moderate priors.

• Journal of Animal Science and Technology
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• 제63권6호
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• pp.1223-1231
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• 2021

Regulator of G protein signaling 16 (RGS16) is known to be associated with porcine circovirus type 2 (PCV2). PCV2 associated disease (PCVAD) is a serious problem in the swine industry. The representative symptoms of PCVAD are high viral titer proliferation and decreased average daily gain. In this study, we identified single nucleotide polymorphisms (SNPs) in the RGS16 region, including the upstream region. Of the 22 identified SNPs, rs332913874, rs326071195, and rs318298586 were genotyped in 142 Yorkshire pigs. These SNPs were significantly associated with the PCV2 viral load. Moreover, the haplotype combination was also related to the PCV2 viral load. The haplotype and diplotype analysis also had a significant difference with the PCV2 viral load. Taken together, our results suggest that RGS16 SNPs considerably affect the PCV2 viral load.

• Genomics & Informatics
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• 제8권2호
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• pp.81-85
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• 2010

The aim of the study was to investigate pig reference families, generated from Korean native pigs (KNP) that were crossed with Yorkshire (YS) breeds, which were used to evaluate genetic markers to select breeding animals with superior pork quality. A set of five candidate genes (PRKAG3, MC4R, CAST, ESR, and PRLR ) was analyzed for association with pork quality traits. PRKAG3 (I199V) SNP genotypes were significantly associated with muscle moisture, protein, and fat contents. The MC4R D298N polymorphism was significantly associated with meat tenderness and color traits. The CAST polymorphism was significantly associated with muscle moisture and crude protein traits. These three genes have been associated with pork quality traits in other pig populations, and some of our results are consistent with earlier studies. In addition, two reproductive candidate genes (ESR and PRLR ) did not have significant associations. These results suggest that further study is warranted to investigate and develop more DNA markers associated with pork quality in our KNP-crossed pig families.

• Journal of Animal Science and Technology
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• 제53권6호
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• pp.525-532
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• 2011

Insufficient pre-slaughter fasting leaves serious amount of feed-like contents (designated here as un-fasted stomach spent feed, USSF) in the eviscerated pig stomach. This study was intended to evoke economical and environmental seriousness of USSF discharge by estimating its value as pig feed. For finishing pigs feeding trial, three levels (0, 5, and 10%) of USSF were blended with pig feed to prepare control and two treatment diets, respectively. A total of 42 (21 males, 21 females) crossbred (Landrace ${\times}$ Yorkshire ${\times}$ Duroc) finishing pigs weighing $81.5{\pm}8.0$ kg were employed to 28d feeding trial and in vivo digestibility trial by $Cr_2O_3$ indicator method with 7 males and 7 female pigs per treatment. In vitro total tract digestion of USSF showed 70.5% and 57.6% of DM and OM digestibilities, respectively which were poorer (p<0.05) than those of pig diet. There were no differences in body weight gain, daily feed intake and feed conversion ratio among treatments although 10% USSF substitution exerted relatively poor performance. In vivo apparent digestibilities of diets containing USSF 5% and 10% were lower (p<0.05) than that of 100% pig feed. There were no differences (p>0.05) in dressing percentage and carcass grade among treatments. Results of this study showed that 5% USSF substitution in finishing pigs diet did not exert any disadvantage in terms of production performance and carcass grade. This study implied that un-fasted slaughter causing excessive excretion of USSF should be avoided. If not avoidable, the USSF should not be wasted in abattoir but could be recycled as pig feed.

• Asian-Australasian Journal of Animal Sciences
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• 제30권2호
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• pp.246-253
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• 2017

Objective: Present study explores the effect of hot summer period on the glycolytic rate of early post-mortem meat quality of Ghungroo and Large White Yorkshire (LWY) pig and comparative adaptability to high temperature between above breeds by shifting the expression of stress related genes like mono-carboxylate transporters (MCTs) and heat shock proteins (HSPs). Methods: Healthy pigs of two different breeds, viz., LYW and Ghungroo (20 from each) were maintained during hot summer period (May to June) with a mean temperature of about $38^{\circ}C$. The pigs were slaughtered and meat samples from the longissimus dorsi (LD) muscles were analyzed for pH, glycogen and lactate content and mRNA expression. Following 24 h of chilling, LD muscle was also taken from the carcasses to evaluate protein solubility and different meat quality measurements. Results: LWY exhibited significantly (p<0.01) higher plasma cortisol and lactate dehydrogenase concentration than Ghungroo indicating their higher sensitivity to high temperature. LD muscle from LWY pigs revealed lower initial and ultimate pH values and higher drip loss compared to Ghungroo, indicating a faster rate of pH fall. LD muscle of Ghungroo had significantly lower lactate content at 45 min postmortem indicating normal postmortem glycolysis and much slower glycolytic rate at early postmortem. LD muscle of LWY showed rapid postmortem glycolysis, higher drip loss and higher degrees of protein denaturation. Ghungroo exhibited slightly better water holding capacity, lower cooking loss and higher protein solubility. All HSPs (HSP27, HSP70, and HSP90) and MCTs (MCT1, MCT2, and MCT4) in the LD muscle of pigs inclined to increase more in Ghungroo than LWY when exposed to high temperature. Conclusion: Effect of high temperature on the variation of HSPs and MCTs may play a crucial role in thermal tolerance and adaptation to different climatic conditions, pH regulation, muscle acidification, drip loss, protein denaturation and also in postmortem meat quality development.