• Journal of Animal Science and Technology
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• v.50 no.4
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• pp.457-464
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• 2008

Heritability and genetic correlation for reproductive traits in Yorkshire pig breed were estimated using Bayesian method via Gibbs sampling. The data set consisted of 9,609 reproductive records at pig breeding farm in Korea. For estimating those parameters using Gibbs sampling, 5,000 cycles of ‘burn-in’ period were discarded among a total of 55,000 samples. Out of the remaining 50,000 samples, 5,000 estimates by each parameter were retained and used for analyses to avoid any correlation among adjacent samples. The reproductive trait considered in this study were total number of born piglets(TNB) and estimated by two different models. The estimated heritability and permanent environmental effect using Gibbs sampler were 0.12±0.020 and 10.9±1.63, respectively. Estimated genetic correlations considered parities as different traits were distributed from 0.99 to －0.13. Such results indicated that reproductive traits for sows should be considered as different traits.

• Food Science of Animal Resources
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• v.40 no.5
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• pp.852-859
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• 2020

The muscle stem cells of domestic animals are of interest to researchers in the food and biotechnology industries for the production of cultured meat. For producing cultured meat, it is crucial for muscle stem cells to be efficiently isolated and stably maintained in vitro on a large scale. In the present study, we aimed to optimize the method for the enrichment of pig muscle stem cells using a magnetic-activated cell sorting (MACS) system. Pig muscle stem cells were collected from the biceps femoris muscles of 14 d-old pigs of three breeds [Landrace×Yorkshire×Duroc (LYD), Berkshire, and Korean native pigs] and cultured in skeletal muscle growth medium-2 (SkGM-2) supplemented with epidermal growth factor (EGF), dexamethasone, and a p38 inhibitor (SB203580). Approximately 30% of total cultured cells were nonmyogenic cells in the absence of purification in our system, as determined by immunostaining for cluster of differentiation 56 (CD56) and CD29, which are known markers of muscle stem cells. Interestingly, following MACS isolation using the CD29 antibody, the proportion of CD56⁺/CD29⁺ muscle stem cells was significantly increased (91.5±2.40%), and the proportion of CD56 single-positive nonmyogenic cells was dramatically decreased. Furthermore, we verified that this method worked well for purifying muscle stem cells in the three pig breeds. Accordingly, we found that CD29 is a valuable candidate among the various marker genes for the isolation of pig muscle stem cells and developed a simple sorting method based on a single antibody to this protein.

• Korean Journal of Agricultural Science
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• v.44 no.1
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• pp.50-59
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• 2017

This study was performed to evaluate the effect of organic medicinal charcoal as a feed additive on aflatoxin absorption, odor emission, fecal microflora and in vitro digestibility in pig diet. A 10-day trial was conducted with 20 [(Yorkshire ${\times}$ Landrace) ${\times}$ Duroc] finishing pigs ($BW=81{\pm}3.3kg$) to investigate the population of Lactobacillus and E. coli in feces and fecal odor ($NH_3$, $H_2S$, total mercaptans, and Acetic acid) in vivo. The in vitro and in vivo treatments included: control (basal diet; CON); (basal diet + 0.25% Organic Medicinal Charcoal; OMC); (basal diet + 0.50% Pyroligneous Charcoal; PC); and (basal diet + 0.50% Coconut tree Charcoal; CC). The aflatoxin absorption capacity was 100, 10, and 20% in OMC, PC, and CC, respectively. The digestibility of dry matter in OMC was significantly higher than that of CON, PC, or CC in vitro (p < 0.05). The digestibility of organic matter in OMC was found to be significantly different from that of CON (p < 0.05). Fecal ammonia and $H_2S$ emissions of OMC were observed to be significantly lower than those of CON, PC, and CC (p < 0.05). Lactobacillus counts in feces of OMC and CC were significantly higher than those of CON and PC (p < 0.05). Fecal E. coli counts of OMC and CC were lower than those of CON and PC (p < 0.05). It was concluded that organic medicinal charcoal can be used as a feed additive in pig diets because it improves the digestibility of feed and fecal odor, and has positive effects on the population of microorganism in feces.

• Journal of Animal Science and Technology
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• v.50 no.3
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• pp.321-330
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• 2008

본 연구는 흑운모 분말 급여시 육성돈에 생산성, 면역관련 혈액학적 지표, 혈청 내 스트레스 농도 및 분내 유해가스 발생에 미치는 영향을 구명하고자 실시하였다. 시험 동물은 [(Landrace ×Yorkshire)×Duroc] 3원 교잡종 육성돈 96두를 공시하였으며, 시험 개시시의 체중은 29.45±1.35kg으로 사양시험을 42일간 실시하였다. 시험설계에서 공시돈은 돈방당 4두씩 1) CON(Basal diet), 2) Solt2(Basal diet+Solto-B 0.2%), 3) Solt4(Basal diet+Solto-B 0.4%) 및 4) Solt6 (Basal diet+Solto-B 0.6%)로 4처리구에 각각 6돈방씩 완전 임의배치 하였다. 질소 소화율에서는 solt4 처리구가 CON과 solt2 처리구에 비해 유의적으로 높게 나타내었다(Linear effect = 0.016). 총 에너지에서는 Solt4와 Solt6 처리구가 CON 처리구에 비해 유의적으로 높게 나타내었다(Linear effect=0.008, Quadratic effect=0.019). 면역 및 스트레스 관련 혈액성상에서는 처리간의 차이가 없었다. 황화수소는 5 day와 10 day에서 대조구에 비해 Solt 처리구가 유의적으로 감소를 하였다(5 day, Linear effect=0.009, Quadractic effect=0.037; 10 day, Linear effect = 0.009). 아세트산는 5 day에서 대조구에 비해 Solt 처리구가 유의적으로 감소를 하였다(Linear effect <0.001, Quadractic effect < 0.001). 15 day에서도 CON과 Solt6 처리구가 Solt2와 Solt4 처리구에 비해 유의적으로 감소를 하였다(Quadractic effect=0.023). 메캅탄은 5 day와 10 day에서 CON 처리구에 비해 Solt 처리구가 유의적으로 감소를 하였다(5 day, Linear effec= 0.029, Quadractic effect=0.037; 10 day, Linear effect=0.029). 암모니아는 5 day, 10 day 및 15 day에서 Solt2와 Solt4 처리구가 CON과 Solt6 처리구에 비해 유의적으로 감소하였다(5 day, Quadractic effect=0.038; 10day, Quadractic effect = 0.045; 15day, Quadractic effect = 0.038). 결론적으로, 육성돈에 흑운모 분말 급여시 분내 유해가스 함량에 유의적인 감소를 나타내었다.

• Animal Bioscience
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• v.36 no.9
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• pp.1336-1349
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• 2023

Objective: The study was conducted to screen differentially expressed miRNAs in sows at early pregnancy by high-throughput sequencing and explore its mechanism of action on embryo implantation. Methods: The blood serum of pregnant and non-pregnant Landrace×Yorkshire sows were collected 14 days after artificial insemination, and exosomal miRNAs were purified for high throughput miRNA sequencing. The expression patterns of 10 differentially expressed (DE) miRNAs were validated by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). The qRT-PCR quantified the abundance of serum exosomal miR-192 in pregnant and control sows, and the diagnostic power was assessed by receiver operating characteristic (ROC) analysis. The target genes of DE miRNAs were predicted with bioinformatics software, and the functional and pathway enrichment analysis was performed on gene ontology and the Kyoto encyclopedia of genes and genomes terms. Furthermore, a luciferase reporter system was used to identify the target relation between miR-192 and integrin alpha 4 (ITGA4), a gene influencing embryo implantation in pigs. Finally, the expression levels of miRNAs and the target gene ITGA4 were analyzed by qRT-PCR, and western blot, with the proliferation of BeWo cells detected by cell counting kit-8 (CCK-8). Results: A total of 221 known miRNAs were detected in the libraries of the pregnant and non-pregnant sows, of which 55 were up-regulated and 67 were down-regulated in the pregnant individuals compared with the non-pregnant controls. From these, the expression patterns of 10 DE miRNAs were validated. The qRT-PCR analysis further confirmed a significantly higher expression of miR-192 in the serum exosomes extracted from pregnant sows, when compared to controls. The ROC analysis revealed that miR-192 provided excellent diagnostic accuracy for pregnancy (area under the ROC curve [AUC]=0.843; p>0.001). The dual-luciferase reporter assay indicated that miR-192 directly targeted ITGA4. The protein expression of ITGA4 was reduced in cells that overexpressed miR-192. Overexpression of miR-192 resulted in the decreased proliferation of BeWo cells and regulated the expression of cell cycle-related genes. Conclusion: Serum exosomal miR-192 could serve as a potential biomarker for early pregnancy in pigs. miR-192 targeted ITGA4 gene directly, and miR-192 can regulate cellular proliferation.

• Asian-Australasian Journal of Animal Sciences
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• v.11 no.4
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• pp.381-384
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• 1998

Twenty-three crossbred (Yorkshire $\times$ Duroc $\times$ Hampshire $\times$ Chester White) primiparous sows were used to evaluate the effects of the mineral-salt laxative in lactation diets on sow and litter performance. The sows were fed a sorghum-extruded soybean-based diet with .85% lysine, .90% Ca, .80% P, and 3.2 Mcal ME/kg. Sow body weight (p > .54) and backfat loss (p > .61), average daily feed intake (p > .42), and litter weight gain (p > .74) were not affected by the mineral-salt laxative in the diet. However, survivability of piglets was greater (p < .06) for sows with the mineral-salt laxative in their diet and, thus, number of pigs weaned was increased. As expected, fecal moisture was increased (p < .09) in sows fed the mineral-salt laxative. Apparent digestibilities of DM, N, and GE were not affected by treatment (p > .26). After weaning, stomachs were collected and scored for ulcers and keratinization using a scoring system of 0 = normal to 3 = severe. Severity of ulceration and keratinization was not significantly affected by treatment (row mean scores differ test p > .25), but scores for sows fed the diet containing the mineral-salt laxative were numerically lower than sows fed the control diet. Thus, our data indicate that sows fed the mineral-salt laxative during lactation had improved piglet survivability, greater fecal moisture, and tended to have fewer lesions in the mucosa of the stomach.

• Asian-Australasian Journal of Animal Sciences
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• v.19 no.11
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• pp.1643-1648
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• 2006

To investigate the effects of feeding restricted on growth, carcass characteristics and plasma profiles in an attempt for optimum responses, a total of 108 cross-bred finishing barrows [(Landrace${\times}$Yorkshire)${\times}$Duroc]weighing an average of $46.88{\pm}0.52kg$ were assigned in a randomized complete block (RCB) design to one of four treatments with three replicates and nine pigs per pen. Feeding regimens were, 1) ad libitum from 50 kg to market weight (Ad 3/3), 2) restricted feeding from 90 kg to market weight (Ad 2/3), 3) restricted feeding from 70 kg to market weight (Ad 1/3), and 4) restricted feeding from 50 kg to market weight (Ad 0/3). During the experimental period, average daily feed intake (ADFI) was decreased from 2.53 kg (AD 3/3) to 2.09 kg (AD 0/3) with increasing restricted feeding duration of (p<0.05). Average daily gain (ADG) of AD 3/3 (0.79 kg) was significantly higher (p<0.05) than those of AD 1/3 (0.74 kg) or AD 0/3 (0.72 kg). Feed efficiency was not influenced by restriction regimens. Blood IGF-I concentrations were increased from 74.14 to 134.25 (167.36-115.66) ng/ml as body weight increased. Blood leptin concentrations were affected by feed intake level and coincided with blood IGF-I concentrations. Most of carcass characteristics were not significantly affected by restricted feeding, however cooking losses in AD 1/3 and Ad 0/3 treatment diet were higher than those in Ad 3/3 and Ad 2/3. In addition, there was a trend that backfat thickness was lowered in proportional to decreasing feed intake (p>0.05). In conclusion, restricted feeding improved feed efficiency after 50 kg body weight without deteriorating the pork quality of barrows.

• Korean Journal of Agricultural Science
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• v.44 no.4
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• pp.519-524
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• 2017

The present study investigated the impacts of mating age of gilts (A1, < 230 days; A2, 230 - 249 days and A3, $250{\leq}days$) on reproductive performance and litter size of sows at farrowing to weaning. A total of 102 crossbred gilts ($Yorkshire{\times}Landrace$; average days old, 90 days) were purchased from a commercial breeding company. After identification of third estrus, the gilts were artificially inseminated using semen of Duroc boars. Pregnant pigs were allotted to one of three groups including A1, A2, and A3. Experimental diets and water were fed ad libitum during each experimental period which included the first, second, and third parities. Backfat thickness (BFT) of sows was higher in A3 at farrowing in the first parity than in others. At weaning time in the second parity, sows in A3 group showed a numerically higher BFT than those in A1. There was no effect of mating age on the total number of piglets born and total weaned piglets. There was a tendency to decrease the wean-to estrus-interval in the first parity as age increased at mating. Results obtained in the present study indicate that the first mating age does not affect the subsequent parites' reproductive performance; however, weaned to estrus interval tended to decrease in A2 at the first parity.

• Journal of the Korean Data and Information Science Society
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• v.27 no.2
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• pp.429-435
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• 2016

Heritability estimates for daily gain (g), backfat thickness (mm), days to 90kg (day), loin eye depth (mm) and meat percent (%) were 0.40, 0.44, 0.40, 0.25 and 0.48, respectively. Estimates of correlation between breeding value and rank for meat productivity traits by model 1 and 2 were 0.995 1.000 and 0.991 1.000, respectively and highly significant (p< 0.0001), and they were almost identical to the breeding values estimated by different farms. When top 5% and top 10% animals were selected by meat productive traits at different farms, markedly different animals were selected by farms since the selected improvement traits in each farm maintaining closed herds were different. Therefore, it seems to be desirable that superior pigs should be selected after the establishment of evaluation system for genetic performance at national level using the integrated data obtained from various farms.

• Journal of Animal Science and Technology
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• v.57 no.1
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• pp.4.1-4.5
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• 2015

The study was performed to investigate the effect of dietary supplementation of a lipid-encapsulated Zinc oxide on growth parameters and intestinal mucosal morphology piglets born to Duroc-sired Landrace ${\times}$ Yorkshire dams. Twenty-four 30-day-old piglets weaned at 25 days of age were orally challenged with $5{\times}10^8$ colony forming units of enterotoxigenic Escherichia coli (ETEC) K88 and fed one of the four diets for 7 days: (i) a nursery basal diet containing 100-ppm ZnO (referred to as BASAL), (ii) BASAL supplemented with 120-ppm apramycin (referred to as ANTIBIO), (iii) BASAL with 2,400-ppm ZnO (referred to as HIGH), and BASAL containing 100-ppm lipid-encapsulated ZnO (referred to as LE). All piglets were killed at the end of the experiment for histological examination on the intestine. The results showed that the average daily gain (ADG), the villus height: crypt depth (CD) ratio in the ileum, and the goblet cell density of the villus and crypt in the duodenum, jejunum, and colon were greater in the LE-fed group that those of the BASAL (p < 0.05). Fecal consistency score (FCS) and the CD ratio in the ileum were less in the LE-fed group, compared to the BASAL-fed one (p < 0.05). The effects observed in the LE-fed group were almost equal to those of the HIGH-fed group as well as even superior to those of the ANTIBIO-fed group. Taken together, our results imply that dietary supplementation of 100-ppm lipid-encapsulated ZnO is as effective as that of 2,400-ppm ZnO for promoting growth diarrhea and intestinal morphology caused by ETEC infection.