• Journal of The Korean Society of Grassland and Forage Science
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• v.32 no.4
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• pp.411-418
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• 2012

This study was conducted to develop the technique for cultivation of forage crops using swine slurry in fallow paddy land located in the mid-mountain area (FPL). The field experiments were carried out from 2007 to 2009 on FPL at Kumsan, Chungbuk province in Korea. Swine slurry was prepared which decayed for 6 months. The experimental plots were consisted of seven treatments; tall fescue-based mixed pasture applied with chemical fertilizer (Control), mono-tall fescue pasture (MTF), tall fescue-based mixed pasture (TFBM), mono-Perennial ryegrass (MPR), mono-Italian ryegrass (MIR), mono-Red clover (MRC and mono), Reed canarygrass (MRCG) applied with swine slurry. The field of tall fescue-based pasture had been sown with a grasses mixture containing 'Fawn' tall fescue, 'potomac' orchardgrass, 'Reveille' Perennial ryegrass, and 'kenblue' Kentuky bluegrass, 'Kenland' Red clover. Seeding rates were 16, 6, 4, 2 and 2 (kg) per ha, respectively. DM yields of forages and rates of grass coverage were higher in MTF, TFBM and MRCG as compared with control treatment. This result means that FPL has contained with favorable conditions for growing grass, because forage productivity is more than 14.5 tons per ha per year in fallow paddy land. In addition, the farmer can save the trouble of repeated plowing and sowing every year, with the introduction of perennial grasses. The farmer must conduct the re-seeding and induce the improvement of management methods for the elevation of the persistence of red clover and perennial ryegrass, because both red clover and perennial ryegrass having high nutritive value and palatability was less persistent. Therefore, we suggest that FPL may be the good land for forage production utilizing swine slurry and swine slurry can be applied on FPL without any negative effects on DM production and the property of soil. FPL of Korea can be better utilized by applying swine slurry to the mono and/or mixed swards.

• Journal of The Korean Society of Grassland and Forage Science
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• v.33 no.2
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• pp.105-110
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• 2013

This study was carried out to investigate the effect of nitrogen (N) fertilization on the forage growth, production and quality of native reed (Phragmites communis) grasses. Field experiments were conducted in Cheonan and in Ansan, 2012. Treatments were control (no N fertilization), 50 kg/ha and 100 kg/ha in Cheonan plots (fertilization on April $30^{th}$, and harvest on June $21^{st}$). Treatments in Ansan plots were control (no N fertilization) and 60 kg/ha (fertilization on May first, and harvest on August first). Forage growth and leaf colors were improved in N fertilized plots. However, the drymatter (DM) percentage was slightly decreased with N fertilization. Forage yields, in terms of DM, crude protein (CP) and digestible DM (DDM), were significantly increased with N fertilization in both sites. In Cheonan, DM, CP and DDM yields per ha were 4,026 kg, 235 kg and 1,850 kg, respectively, in the control plot, and were 4,658 kg, 306 kg and 2,388 kg, respectively, in the N 50 kg plot, and 5,622 kg, 446 kg and 3,143 kg, respectively, in the N 100 kg plot. In Ansan, DM, CP and DDM yields per ha were 2,802 kg, 177 kg and 1,288 kg, respectively, in the control plot, and were 3,876 kg, 294 kg and 1,853 kg, respectively, in the N 60 kg plot. Forage quality in terms of CP content, in vitro DM digestibility (IVDMD) and relative feed value (RFV) were also increased with N fertilization in both sites. In Cheonan, the CP content, IVDMD and RFV were 5.85%, 45.96% and 64.5 (grade 5), respectively, in the control plot, 6.58%, 51.27% and 72.3 (grade 5), respectively, in the N 50 kg plot, and 7.94%, 55.91% and 72.7 (grade 5), respectively, in the N 100 kg plot. In Ansan, the CP content, IVDMD and RFV were 6.30%, 45.98% and 70.2 (grade 5), respectively, in the control plot, and 7.59%, 47.80% and 78.3 (grade 4), respectively, in the N 60 kg plot. In conclusion, N fertilization of 60~100 kg/ha was desirable for greater forage production, with a higher quality of native Phragmites communis achievable. This should only be applied if the fertilization area is not located at a riverside/streamside or in riparian land where there is a high risk of water pollution by fertilization.

• Microbiology and Biotechnology Letters
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• v.33 no.2
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• pp.117-122
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• 2005

Beijerinckia indica was cultured in mineral salts medium (MSM) medium with various carbon and nitrogen sources to improve the production yield of heteropolysaccharide-7 (PS-7). At high C/N ratio, the high concentration of PS-7 was produced until 40 h of the culture, whereas most of the glucose as a carbon source was used for the cell growth at low C/N ratio. However, at the high C/N ratio, PS-7 accumulation stopped at 48 h of the culture due to the increasing viscosity of the culture broth would inhibit the cell growth. Therefore, the optimized value of C/N ratio was 33.3 (20 g/L glucose, 7.5 mM $NH_{4}NO_3$) for the high production of PS-7. In the culture with various carbon sources, B. indica effectively used the hexoses or glucose-generating sugars for PS-7 formation. Especially, sucrose was the best carbon source for the high production of PS-7 (6.96 g/L) with a high viscosity (40772 cp). In the culture of B. indica with MSM medium containing 20 g/L glucose and 7.5 mM $NH_{4}NO_3$ in a 51 fermentor, the highest cell concentration was 2.5 g/L and the highest concentration of PS-7 was 7.5 g/L (35174 cp). The additional nitrogen sources of 7.5 mM $NH_{4}NO_3$, glutamine and glutamate at 12 h of the culture after exhaustion of a nitrogen source regulated the metabolism of carbon sources, therefore the nitrogen sources could control PS-7 synthesis.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.62 no.2
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• pp.143-148
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• 2017

Soybean [Glycine max (L.) Merr.] seed is an important dietary source of protein, oil, carbohydrates, isoflavones, and other nutrients for humans and animals. Raffinose and stachyose are the main antinutritional factors in soybean seed. They are carbohydrates belonging to the raffinose family of oligosaccharides, which are not readily digested in humans and cause flatulence or diarrhea. The genetic reduction of the raffinose and stachyose contents in mature soybean seeds will improve the nutritional value of soybean. The objective of this research was to evaluate agronomic traits with 10 $F_6$ strains selected from breeding populations derived from a cross among seven parents. The contents of raffinose and stachyose in mature seeds were detected by high-performance liquid chromatography. Agronomic traits such as flower color, flowering date, harvesting date, lodging, plant height, seed coat color, hilum color, 100 seed weight, and yield were evaluated. Ten intermediate parents showed low raffinose and stachyose contents. The intermediate parent 883-1 had a small seed size, six intermediate parents (15A1, 15D1, RS-5, RS-33, RS-64, and RS-70) had a medium seed size, and two intermediate parents (14G20 and RS-21) had a large seed size. The intermediate parent RS-21 had a black seed coat and a green cotyledon. Four intermediate parents (883-1, 14G20, RS-5, and RS-21) had elite agronomic traits. The new intermediate parents developed through this study will be used to develop improved soybean cultivars with low contents of raffinose and stachyose.

• Korean Journal of Agricultural and Forest Meteorology
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• v.21 no.4
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• pp.250-260
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• 2019

This study assessed clove germination, shoot growth, photosynthesis and bulb development of southern-type garlic (Allium sativum L.) in a temperature gradient tunnel (TGT), to examine the impacts of increases in temperature on the growth of garlic and find a way to minimize them. The temperatures in the middle and outlet of the TGT were 3.2℃ and 5.8℃ higher, respectively, than the ambient temperature at the tunnel inlet. The germination of garlic cloves was late at temperatures of ambient+3℃ (in the middle of the TGT) and ambient+6℃ (at the outlet) than at ambient temperature (at the inlet). However, bolting and the timing of maximum leaf number per plant were faster at ambient+3℃ or +6℃ than at ambient temperature. Shoot growth was generally greater at ambient temperature. Bulb growth did not significantly differ according to cultivation temperatures, but fresh and dry weights were slightly higher at ambient temperature and ambient+3℃ in the late growth stage. The photosynthesis rate (A), stomatal conductance (g_s), and transpiration rate (E) were higher at ambient+3℃ than at ambient temperature. Furthermore, at ambient+3℃, the net photosynthetic rate (A_max) was high, while the dark respiration rate (R_d) was low. At ambient temperature and ambient+3℃, bulb development was healthier, resulting in better productivity and more commercial bulbs, while at ambient+6℃, the bulbs were small and secondary cloves developed, resulting in low commercial value. Therefore, at elevated temperatures caused by global warming, it is necessary to meet the low-temperature requirements before clove sowing, or to delay the sowing time, to improve germination rate and increase yield. The harvest should also be advanced to escape high-temperature stress in the bulb development stage.

• Journal of Animal Environmental Science
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• v.13 no.1
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• pp.13-20
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• 2007

Sawdust biofiltration is an emerging bio-technology for control of ammonia emissions including compost odors from composting of biological wastes. Although sawdust is widely used as a medium for bulking agent in composting system and for microbial attachment in biofiltration systems, the performance of agitated bed composting and sawdust biofiltration are not well established. A pilot-scale composting of hog manure amended with sawdust and sawdust biofiltration systems for practical operation were investigated using aerated and agitated rectangular reactor with compost turner and sawdust biofilter operated under controlled conditions, each with a working capacity of approximately $40m^3\;and\;4.5m^3$ respectively. These were used to investigate the effect of compost temperature, seed germination rate and the C/N ratio of the compost on ammonia emissions, compost maturity and sawdust biofiltration performance. Temperature profiles showed that the material in three runs had been reached to temperature of 55 to $65^{\circ}C$ and above. The ammonia concentration in the exhaust gas of the sawdust biofilter media was below the maximum average value as 45 ppm. Seed germination rate levels of final compost was maintained from 70 to 93% and EC values of the finished compost varied between 2.8 and 4.8 ds/m, providing adequate conditions for plant growth.

• Food Science and Preservation
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• v.21 no.1
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• pp.62-68
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• 2014

This study was performed in order to investigate the functional components of 5 kinds of marine algae. We have collected 5 samples of marine algae, such as the sea mustard (Undaria pinnatifida), sea tangle (Laminaria iaponice), sea weed fusiforme (Hizikia fusiforme), green laver (Entetomotpha), laver (Phophyratenera), which have been harvested in Jeollanam-do. In order to examine the functional effects, 5 kinds of marine algae were extracted with hot water ($80^{\circ}C$, 4 hr), ethanol and methanol (R.T., 4 hr), and subcritical water extract (SWE, 3 MPa, $90^{\circ}C$, $150^{\circ}C$, $210^{\circ}C$). A higher yield of extract was obtained through SWE method (3 MPa, $210^{\circ}C$) in all of the samples obtained. The highest total sugar content was 427.4 mg/g in green laver extracted with SWE (3 MPa, $210^{\circ}C$). The content of the SWE total phenolic compounds was higher than that of the water and solvent (methanol, ethanol) extracts. The anti-oxidative activities of the extracts from 5 kinds of marine algae were examined through the DPPH radical scavenging activity test. The SWE (3 MPa, $150^{\circ}C$ and $210^{\circ}C$) of the marine algae was the highest among all of the extracts. As per the results, the SWE of the marine algae contained more functional components and it had a higher antioxidant activity than those of the other extracts. The $IC_{50}$ value of tyrosinase in seaweed fusiforme and laver were higher than those of the other samples. These results strongly support the possible use of marine algae as functional materials.

• Korean Journal of Poultry Science
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• v.47 no.1
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• pp.21-27
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• 2020

This study intended to investigate the effects of slaked lime and ethanol on poultry red mites (PRM) in the coops of laying hen. Three experiments were conducted to this effect. The first used untreated hydrated lime as a control and 10% and 20% aqueous solutions of hydrated lime were used as treatments. The second experiment used 30%, 40%, 50%, 60%, 70%, 80%, 90%, and 100% ethanol solutions as treatments. The third experiment used hydrated lime in two distinct concentrations (10% and 20%) and 30%, 40%, and 50% ethanol solutions mixed to yield a total of 6 (2 × 3) treatments. All treatments were tested three times each. In the first experiment, the PRM killing rates were 74.0% and 92.3% when treated with 10% and 20% hydrated lime solutions, respectively. The acaricidal activity of the control sample was 0%. In the second experiment, the lowest value was 1.67% for the 30% ethanol solution, 8.33% for the 40% solution, and 7.47% for the 50% solution. The acaricidal activities of the 60% and 70% solutions were 42.4% and 84.7%, respectively. A 100% acaricidal activity was observed in dilutions above 80% (P<0.05). In the third experiment, the PRM killing effect of the hydrated lime + ethanol mixture was 100% in all treatments. In conclusion, after taking into consideration the economical and safety factors, a mixture of 10% hydrated lime solution and 30% ethanol is considered to be a suitable candidate for controlling PRM.

• Journal of Korean Society of Environmental Engineers
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• v.28 no.5
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• pp.501-510
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• 2006

To investigate the effects of MLSS concentration and influent C/N ratio on the nitrogen removal efficiency of alternately intermittently aerated nonwoven fabric filter bioreactors, the MLSS concentrations of the reactors were maintained at approximately 5,500 mg/L, 10,000 mg/L and 15,000 mg/L, and the influent TCOD/TKN ratio was decreased gradually from 5 to 2 by adding $NH_4Cl$. The influent was prepared by diluting a food waste leachate to a TCOD concentration of about 300 mg/L. The results of the experiment showed F/M ratios less than 0.112 g TCOD/g MLSS-day, average TCOD removal efficiencies of above 95%, and an average observed microbial yield coefficient of 0.283 g MLSS/g COD removed. The nitrification efficiencies were computed to be always better than 96% except one case where the nitrification efficiency was 90.5% when the MLSS concentration and the influent TCOD/TKN ratio was 5,500 mg/L and 2, respectively. The denitrification efficiency deteriorated as the influent TCOD/TKN ratio decreased. The average denitrification efficiency at the MLSS concentration of 10,000 mg/L was 10.7% better than that at the MLSS concentration of 5,500 mg/L, and the denitrification rate improved at a rate of 2.66 mg NL as the MLSS concentration increased by 1,000 mg/L. When the MLSS concentration was 15,000 mg/L, however, the average denitrification efficiency was merely 4.6% higher compared to when the MLSS concentration was 5,500 mg/L, and the denitrification rate increased at a rate of 0.75 mg N/L per 1,000 mg/L MLSS increase. Therefore, no strict proportional relationship was found between MLSS concentration and endogenous denitrification rate. The average alkalinity consumption rate was 3.36 mg alkalinity/mg T-N removed, which is similar to the theoretical value of 3.57 mg alkalinity/mg T-N removed, but the rate increased as the influent TCOD/TKN ratio decreased.

• Journal of Korean Society of Environmental Engineers
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• v.35 no.3
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• pp.165-170
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• 2013

In this study, physico-chemical properties and environmental fate were investigated and ecotoxicity tests using fish, daphnia and algae were conducted for an initial ecological risk assessment of 1,2-Benzisothiazol-3-one. Due to low volatility of the test substance under environmental conditions, it is likely to distributed in soil and water environment. The compound has low adsorption in the soil, with low bioconcentration potential. Acute toxicity results showed that 96 h-$LC_{50}$ for Oryzias laties was 4.7 mg/L (measured) and 48h-$EC_{50}$ for Daphnia magna was 3.3 mg/L (measured). In a growth inhibition test with Pseudokirchneriella subcapitata, 72 h-$EC_{50}$ was 0.456 mg/L (growth rate, nominal) and 0.262 mg/L (yield, nominal). Using the acute toxicity value of algae, predicted no-effect concentration (PNEC) in the aquatic environment was determined to be 2.62 ${\mu}g/L$ using an factor of 100. According to globally harmonized system (GHS), the compound was categorized as aquatic acute 1 for algae, while it was categorized as aquatic acute 2 for fish and daphnia. This screening assessment suggests that the test substance may pose ecological risks in the aquatic environment.