• Title/Summary/Keyword: Yield loss

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Biosynthesis of Cephalexin in PEG400-Ammonium Sulfate and PEG400-Magnesium Sulfate Aqueous Two-Phase Systems

  • Cao, Xuejun;Zhu, Jianhang;Wei, Dongzhi;Hur, Byung-Ki
    • Journal of Microbiology and Biotechnology
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    • v.14 no.1
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    • pp.62-67
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    • 2004
  • The biosynthesis of cephalexin was carried out in the aqueous two-phase systems using penicillin acylase as a catalyst, and 7-aminodeacetoxicephalosporanic acid (7-ADCA) and phenylglycine methyl ester (PGME) as substrates. 20% PEG400-l7.5% ${(NH_4)}_2SO_4$ containing 0.5 M NaCl and 1.5 M methanol aqueous two-phase systems (ATPS) were selected as reaction medium, and 53% product yield was obtained using immobilized penicillin acylase as a catalyst. 20% PEG400-l5% $MgSO_4$ ATPS was also used for the synthesis of cephalexin, and 60-62% product yield was obtained by using free penicillin acylase as a catalyst. When batch reactions were repeated in the ATPS, the cephalexin yields decreased during the reactions due to deactivation, loss, and product inhibition of penicillin acylase. The effect of different ratio of phenylglycine methyl ester to 7-ADCA on the product yield was investigated, and high cephalexin yield was obtained at a high molar ratio.

A Study on Soot Formation of Turbulent Premixed Propane Flames in n Constant-Volume Combustor at High Temperatures and High Pressures (고온ㆍ고압 정적 연소기내 난류 프로판 예혼합 화염의 매연생성에 관한 연구)

  • 배명환
    • Transactions of the Korean Society of Automotive Engineers
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    • v.9 no.4
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    • pp.1-9
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    • 2001
  • The soot yield has been studied by a premixed propane-oxygen-inert gas combustion in a specially designed disk-type constant-volume combustion chamber to investigate the effects of pressure, temperature and turbulence on soot formation. Premixtures are simultaneously ignited by eight spark plugs located on the circumference of chamber at 45 degree intervals in order to observe the soot formation under high pressures. The eight flames converged compress the end gases to a high pressure. The laser schlieren and direct flame photographs for observation field with 10 mm in diameter are taken to examine into the behaviors of flame front and gas flow in laminar and turbulent combustion. The soot volume fraction in the chamber center during the final stage of combustion at the highest pressure is measured by the in situ laser extinction technique and simultaneously the corresponding burnt gas temperature by the two-color pyrometry method. The pressure and temperature during soot formation are changed by varying the initial charge pressure and the volume fraction of inert gas compositions, respectively. It is found that the soot yield increases with dropping temperature and rising pressure at constant equivalence ratio, and that the soot yield of turbulent combustion decreases in comparison with that of laminar combustion because the burnt gas temperature increases with the drop of heat loss.

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Applicability Examination of the RUSLE Sediment Yield Prediction Equation in Korea (해외 토사유출량 산정공식의 국내적용성 검토 (I);RUSLE를 중심으로)

  • Son, Gwang-Ik
    • Journal of Korea Water Resources Association
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    • v.34 no.3
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    • pp.199-207
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    • 2001
  • Due to the nationwide development, sediment yield has to be estimated to design the sedimentation basin which is used to minimize the effects of construction or disturbing the natural soil condition. But there is no proved equation for the estimation of sediment yield in Korea. Therefore, applicability and the limitation of RUSLE (revised universal sediment loss equation) sediment yield equation is examined for the construction sites, where the rainfall and sediment data are available. General mistakes in estimation of the RUSLE parameters are also discussed. It is found that RUSLE could be applied in Korea as long as the sediment delivery ratio was considered. Appropriate estimation method of sediment delivery ratio are also proposed.

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Evaluation of Cyst Loss in Standard Procedural Steps for Detecting of Giardia lamblia and Cryptosporidium parvum in Water

  • Kim, Kyung-Ju;Jung, Hyang-Hee;Lee, Ki-Say
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.11 no.4
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    • pp.368-371
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    • 2006
  • The standard procedure outlined by the United States Environmental Protection Agency (US EPA) in Method 1623 for analyzing Giardia lamblia cysts and Cryptosporidium parvum oocysts in water samples consists of filtration, elution, centrifugal concentration, immunomagnetic separation (IMS), and immunofluorescence assay (IFA) followed by microscopic examination. In this study, the extent of (oo)cyst loss in each step of this procedure was evaluated by comparing recovery yields in segmented analyses: (i) IMS + IFA, (ii) concentration + IMS + IFA, and (iii) filtration/elution + concentration + IMS + IFA. The complete (oo)cyst recovery by the full procedure was $52{\sim}57%$. The (oo) cyst loss in the IMS step was only $0{\sim}6%$, implying that IMS is a fairly reliable method for (oo)cyst purification. Centrifugal concentration of the eluted sample and pellet collection before IMS resulted in a loss of $8{\sim}14%$ of the (oo)cysts. The largest (oo)cyst loss occurred in the elution step, with $68{\sim}71%$ of the total loss. The permeated loss of (oo)cysts was negligible during filtration of the water sample with a $1.0-{\mu}m$ pore polyethersulfone (PES) capsule. These results demonstrated that the largest fraction of (oo)cyst loss in this procedure occurred due to poor elution from the filter matrix. Improvements in the elution methodology are therefore required to enhance the overall recovery yield and the reliability of the detection of these parasitic protozoa.

Studies on the Reduction of Hydrogen Fluoride Damage to Rice Plant I. Effect of Soil Improvement Agents (수도(水稻)에 대(對)한 불화수소(弗化水素) 가스 피해경감(被害輕減)에 관(關)한 연구(硏究) I. 개량제(改良劑) 처리(處理)에 의(依)한 효과(效果))

  • Kim, Bok-Young;Han, Ki-Hak;Kim, Jeong-Je
    • Korean Journal of Soil Science and Fertilizer
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    • v.14 no.3
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    • pp.157-162
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    • 1981
  • The studies we re carried out to examine the effect of soil improvement agents, such as lime, wollastonite and phosphate, in reducing the damage caused by hydrogen fluoride gas to the vegetative growth of rice. The yield loss, damage rate of the plant leaves, the fluoride of the plants were measured. The results obtained are summarized as follows : 1. The best results were obtained a treatment of lime and wollastonite. 2. The rice yield was not significantly increased by the soil improvement agents. 3. The fluoride content of the plants was positively correlated with yield loss and damage rate during vegetative growth.

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Past and Present Meteorological Stress in Crop Production and Its Significance (농작물의 기상재해와 대책)

  • Eun-Woong Lee
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.27 no.4
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    • pp.291-295
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    • 1982
  • The biosphere of the earth is not only about to overpass the limit to meet the food demand of the world but also the stability of its food production has been also jeopardized by the disasters and pests, especially by the unpredictable weather disasters. In addition the agricultural and industrial pollution against biosphere aggravates the unstability of agricultural production and constitutes a threat in securing the food of the world. In Korea the yield level of crops has been greatly enhanced by the improved agrotechnologies and varietal improvement, but the yield variability due to unfavorable weather events and pests remained unchanged with the change in time. Among weather-related disasters the drought and flood damages has occurred most frequently and impacted most greatly on the agricultural production and its stability. During last decade (1970-l980) the rice production experienced the average annual loss of 0.544 million metric ton which was composed of 0.21 million M/T by climatic disaster, 0.21 million M/T by disease and 0.12 million M/T by insects, and the annual loss of upland crop production from climatic disasters amounted to 0.06 million metric tons. Especially in 1980, the global climatic disasters due to cold or hot temperature endangered the agricultural production all over the world and also the rice production of Korea recorded the unprecedented yield reduction of about 30 percent due to cool summer weather. Nowadays, the unusual weather conditions are prevaling throughout the world, and agro-meteologists predict that the unpredictable cool summer and drought will often attack the rice and other crops in 1980's. To meet the coming weather unstability and to secure the stable crop production, multilateral efforts should be rendered. Therefore, the Korea Society of Crop Science, which commemorates the 20th anniversary of its founding, prepared the symposium on Meteological Stress in Crop Production and its Countermeasures to discuss the decrease in agricultural production due to weather-related disasters and to devise the multilateral counter-measures against the unfavorable weather events.

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Yield Loss Assessment and Economic Thresholds of Squash Powdery Mildew Caused by Sphaerotheca fuliginea (호박 흰가루병의 피해 해석 및 경제적 방제수준 설정)

  • Moon, Youn-Gi;Choi, Jun-Keun;Kang, An-Seok
    • Research in Plant Disease
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    • v.16 no.3
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    • pp.285-289
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    • 2010
  • The experiments were carried out in fields for two years from 2008 to assess yield losses of squash due to powdery mildew caused by Sphaerotheca fuliginea and to determine its economic thresholds. Powdery mildew disease was first observed in late June, about 50 days after field-transplanting, progressed rapidly during late July to early August, and began to reduce from late August. Powdery mildew severity was negatively correlated with squash yields. A positive correlation was observed between fruit weight and % marketable fruits. A simple linear regression model was obtained as Y=-10.399 X + 6607.5 with $R^2$ = 0.9700 when squash yields (Y) was predicted using powdery mildew severity as an independent variable(X). Spray threshold for maximizing squash yields without economic considerations was estimated as 6.5% in terms of leaf lesion area with powdery mildew. Economic threshold and economic spray threshold able to compensate the costs of fungicide sprays were determined as 21.6% and 17.3% in leaf lesion area, respectively.

Mulberry Growth Promotion by Nitrogen Application under Abnormally Wet and Cool Weather Conditions (하추기 이상 저온하에서 뽕나무 발육부진요인과 추비에 의한 생육증진)

  • 이원주;윤명근
    • Journal of Sericultural and Entomological Science
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    • v.36 no.2
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    • pp.110-114
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    • 1994
  • Abnormally cool and wet weather conditions during the summer of 1993 offered an opportunity to evaluate the effects of abnormal weather conditions on mulberry growth and to develop cultivation practicies to reduce leaf yield loss under the similar abnormal weather conditions. Different methods of nutrient supplementation were evaluated in Suwon and Kongju. Nitrogen was supplemented by foliar spray of urea(1.7%) or composite chemical fertilizer Jamsibiryo #8, or by the application of ammonium sulfate(60kg/ha) to the soil. During the period between the late June and the early September which corresponds to the mulberry growing season after summer pruning, mean temperature was 1.4$^{\circ}C$ lower and precipitation 83mm higher than normal year. This weather condition in 1993 caused reduction in leaf yield by 16.4% than common year. Soil nitrogen content decreased due to higher precipitation. Leaf yield loss was reduced by the supplementation of nitrogen to the soil. Leaf nitrogen content increased with the foliar urea spray and nitrogen supplementation to the soil.

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Comparison of Soil Loss Estimation using SWAT and SATEEC (SWAT과 SATEEC 모형을 이용한 토양유실량 비교)

  • Park, Youn-Shik;Kim, Jong-Gun;Heo, Sung-Gu;Kim, Nam-Won;Lim, Kyung-Jae
    • Proceedings of the Korea Water Resources Association Conference
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    • 2008.05a
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    • pp.1295-1299
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    • 2008
  • Soil erosion is a natural process and has been occurring in most areas in the watershed. However, accelerated soil erosion rates have been causing numerous environmental impacts in recent years. To reduce soil erosion and sediment inflow into the water bodies, site-specific soil erosion best management practices (BMPs) need to be established and implemented. The most commonly used soil erosion model is the Universal Soil Loss Equation (USLE), which have been used in many countries over 30 years. The Sediment Assessment Tool for Effective Erosion Control (SATEEC) ArcView GIS system has been developed and enhanced to estimate the soil erosion and sediment yield from the watershed using the USLE input data. In the last decade, the Soil and Water Assessment Tool (SWAT) model also has been widely used to estimate soil erosion and sediment yield at a watershed scale. The SATEEC system estimates the LS factor using the equation suggested by Moore and Burch, while the SWAT model estimates the LS factor based on the relationship between sub watershed average slope and slope length. Thus the SATEEC and SWAT estimated soil erosion values were compared in this study. The differences in LS factor estimation methods in the SATEEC and SWAT caused significant difference in estimated soil erosion. In this study, the difference was -51.9%(default threshold)$\sim$-54.5%(min. threshold) between SATEEC and non-patched SWAT, and -7.8%(default threshold)$\sim$+3.8%(min. threshold) between SATEEC and patched SWAT estimated soil erosion.

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Marination and Physicochemical Characteristics of Vacuum-aged Duck Breast Meat

  • Khan, Muhammad Issa;Lee, Hyun Jung;Kim, Hyun-Joo;Young, Hae In;Lee, Haelim;Jo, Cheorun
    • Asian-Australasian Journal of Animal Sciences
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    • v.29 no.11
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    • pp.1639-1645
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    • 2016
  • We investigated marinade absorption and physicochemical characteristics of vacuum-aged duck breasts that were halved and individually vacuum-packed for chiller aging at $4^{\circ}C$ for 14 d. One half was marinated for 0, 7, or 14 d, while the second half was used as a control. Marinade absorption, cooking loss, cooking yield, texture profile, pH, color, protein solubility, and thiobarbituric acid reactive substances (TBARS) values were evaluated, and protein sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was performed. Marinade absorption and pH did not vary significantly after 14 d of aging. Marination increased the pH, color ($a^*$ and $b^*$) values, and cooking yield and reduced cooking loss. TBARS values significantly increased with aging time, but were significantly reduced by marination. Myofibril and total protein solubility increased with aging and marination, while SDS-PAGE showed protein degradation. Hence, aging and marination can be used simultaneously to improve physicochemical quality and cooking yield of vacuum-aged duck breast.