• Journal of Ginseng Research
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• v.44 no.3
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• pp.399-404
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• 2020

Korean ginseng (Panax ginseng) is associated with a variety of therapeutic effects, including antioxidative, anti-inflammatory, vasorelaxative, antiallergic, antidiabetic, and anticancer effects. Accordingly, the use of ginseng has reached an all-time high among members of the general public. However, the safety and efficacy of ginseng in transplant recipients receiving immunosuppressant drugs have still not been elucidated. Transplantation is the most challenging and complex of surgical procedures and may require causation for the use of ginseng. In this regard, we have previously examined the safety, immunological benefits, and protective mechanisms of ginseng with respect to calcineurin inhibitor-based immunosuppression, which is the most widely used regimen in organ transplantation. Using an experimental model of calcineurin inhibitor-induced organ injury, we found that ginseng does not affect drug levels in the peripheral blood and tissue, favorably regulates immune response, and protects against calcineurin inhibitor-induced nephrotoxicity and pancreatic islet injury. On the basis of our experimental studies and a review of the related literature, we propose that ginseng may provide benefits in organ transplant recipients administered calcineurin inhibitors. Through the present review, we aimed to briefly discuss our current understanding of the therapeutic benefits of ginseng related to transplant patient survival.

• Toxicological Research
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• v.33 no.1
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• pp.43-48
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• 2017

With ultraviolet and visible light exposure, some pharmaceutical substances applied systemically or topically may cause phototoxic skin irritation. The major factor in phototoxicity is the generation of reactive oxygen species (ROS) such as singlet oxygen and superoxide anion that cause oxidative damage to DNA, lipids and proteins. Thus, measuring the generation of ROS can predict the phototoxic potential of a given substance indirectly. For this reason, a standard ROS assay (ROS assay) was developed and validated and provides an alternative method for phototoxicity evaluation. However, negative substances are over-predicted by the assay. Except for ultraviolet A (UVA), other UV ranges are not a major factor in causing phototoxicity and may lead to incorrect labeling of some non-phototoxic substances as being phototoxic in the ROS assay when using a solar simulator. A UVA stimulator is also widely used to evaluate phototoxicity in various test substances. Consequently, we identified the applicability of a UVA simulator to the ROS assay for photoreactivity. In this study, we tested 60 pharmaceutical substances including 50 phototoxins and 10 non-phototoxins to predict their phototoxic potential via the ROS assay with a UVA simulator. Following the ROS protocol, all test substances were dissolved in dimethyl sulfoxide or sodium phosphate buffer. The final concentration of the test solutions in the reaction mixture was 20 to $200{\mu}M$. The exposure was with $2.0{\sim}2.2mW/cm^2$ irradiance and optimization for a relevant dose of UVA was performed. The generation of ROS was compared before and after UVA exposure and was measured by a microplate spectrophotometer. Sensitivity and specificity values were 85.7% and 100.0% respectively, and the accuracy was 88.1%. From this analysis, the ROS assay with a UVA simulator is suitable for testing the photoreactivity and estimating the phototoxic potential of various test pharmaceutical substances.

• Food Science of Animal Resources
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• v.35 no.6
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• pp.721-730
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• 2015

The objective of this study was to examine the effect of ginger extract (GE) combined with citric acid on the tenderness of duck breast muscles. Total six marinades were prepared with the combination of citric acid (0 and 0.3 M citric acid) and GE (0, 15, and 30%). Each marinade was sprayed on the surface of duck breasts (15 mL/100 g), and the samples were marinated for 72 h at 4℃. The pH and proteolytic activity of marinades were determined. After 72 h of marination, Warner Bratzler shear force (WBSF), myofibrillar fragmentation index (MFI), pH, cooking loss, moisture content, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and protein solubility were evaluated. There was no significant (p>0.05) difference in moisture content or cooking loss among all samples. However, GE marination resulted in a significant (p<0.05) decrease in WBSF but a significant (p<0.05) increase in pH and MFI. In addition, total protein and myofibrillar protein solubility of GE-marinated duck breast muscles in both WOC (without citric acid) and WC (with citric acid) conditions were significantly (p<0.05) increased compared to non-GE-marinated duck breast muscles. SDS-PAGE showed an increase of protein degradation (MHC and actin) in WC condition compared to WOC condition. There was a marked actin reduction in GE-treated samples in WC. The tenderization effect of GE combined with citric acid may be attributed to various mechanisms such as increased MFI and myofibrillar protein solubility.

• Food Science of Animal Resources
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• v.35 no.6
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• pp.783-792
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• 2015

Abstract This study evaluated the effects of adding glasswort hydrate containing non-meat ingredient (GM, carboxy methyl cellulose; GC, carrageenan; GI, isolated soy protein; GS, sodium caseinate) on the quality characteristics of reduced-salt, reduced-fat frankfurters. The pH and color evaluation showed significant differences, depending on the type of glasswort hydrate added (p<0.05). In the raw batters and cooked frankfurters, the addition of glasswort hydrate decreased the redness and increased the yellowness in comparison with frankfurters without glasswort hydrate. The reduction in salt and fat content significantly increased cooking loss and decreased hardness, tenderness and juiciness (p<0.05). Glasswort hydrate containing non-meat ingredient improved cooking loss, water holding capacity, emulsion stability, hardness, and viscosity of reduced-salt, reduced-fat frankfurters. The GM treatment had the highest myofibiliar protein solubility among all treatments, which was associated with emulsion stability and viscosity. The GC treatment had higher values for all texture parameters than the control. In the sensory evaluation, the addition of glasswort hydrate with non-meat ingredient improved tenderness and juiciness of reduced-salt, reduced-fat frankfurters. GM, GC, and GI treatments improved not only the physicochemical properties but also the sensory characteristics of reduced-salt, reduced-fat frankfurters. The results indicated that the use of glasswort hydrate containing non-meat ingredient was improved the quality characteristics of reduced-salt, reduced-fat frankfurters.

• The Journal of Korean Academy of Prosthodontics
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• v.31 no.4
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• pp.580-610
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• 1993

The purpose of this study was to analyse the deflection and stress distribution at the supporting bone and it's superstructure by the alteration of angulation between implant and it's implant abutment. For this study, the free-end saddle case of mandibular first and second molar missing would be planned to restore with fixed prosthesis. So the mandibular second premolar was prepared for abutment, and the cylinder type osseointegrated implant was placed at the site of mandibular second molar for abutment. The finite element stress analysis was applied for this study. 13 two-dimensional FEM models were created, a standard model at $0^{\circ}$ and 12 models created by changing the angulation between implant and implant abutment as increasing the angulation mesially and distally with $5^{\circ}$ unittill $30^{\circ}$. The preprocessing decording, solving and postprocessing procedures were done by using FEM analysis software PATRAN and SUN-SPARC2GX. The deflections and von Mises stresses were calculated under concentrated load (load 1) and distributed load(load 2) at the reference points. The results were as follows : 1. Observing at standard model, the amount of total deflection at the distobuccal cusp-tip of pontic under concentrated load was largest of all, and that at the apex of implant was least of all, and the amount of total deflection at the buccal cusp-tip of second premolar under distributed load was largest of all, and that at the apex of implant was least of all. 2. Increasing the angulation mesially or distally, the amounts of total deflection were increased or decreased according to the reference points. But the order according to the amount of total deflection was not changed except apex of second premolar and central fossa of implant abutment under concentrated load during distal inclination. 3. Observing at standard model, the von Mises stress at the distal joint of pontic under concentrated load was largest of all, and that at the apex of implant was least of all. The von Mises stress at the distal margin of second premolar under distributed load was largest of all, and that at the apex of Implant was least of ail. 4. Increasing the angulation of implant mesially, the von Mises stresses at the mesial crest of implant were increased under concentrated load and distributed load, but those were increased remarkably under distributed load and so that at $30^{\circ}$ mesial inclination was largest of all. 5. Increasing the angulation of implant distally, the von Mises stresses at the distal crest of implant were increased remarkably under concentrated load and distributed load, and so those at $30^{\circ}$ distal inclination were largest of all.

• Journal of Soil and Groundwater Environment
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• v.17 no.2
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• pp.54-61
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• 2012

In this study, the adsorption of $Cu^{2+}$ from aqueous solution by the biochar derived from woody biomass at different pyrolysis temperatures has been investigated. The woody biomass wastes used in this study were branch of willow ($Salix$ $koreensis$ $Andersson$) and bark of chestnut ($Castanea$ $crenata$ $var.$ $dulcis$). Three biochar samples prepared by heating each biomass at temperature of $300^{\circ}C$, $500^{\circ}C$, and $700^{\circ}C$were tested for the adsorption capacity of Cu. Also the physicochemical properties of the developed biochars were studied using different characterization techniques such as FT-IR, SEM, BET surface area, and cation exchange capacity (CEC). The adsorption of Cu could be well described by Langmuir model for both willow and chestnut biochars with $R^2{\geq}0.98$. The maximum adsorption capacities of the biochar produced at $700^{\circ}C$ from the Langmuir equation were found to be 12.5 mg $g^{-1}$ and 16.9 mg $g^{-1}$ for willow and chestnut, respectively. Chestnut biochar was found to interact more effectively with the active sites available for Cu, resulting higher removal of Cu(II) than wiloow biochar. Ion exchange and surface complexation found to be the main mechanisms involved in the adsorption process. This study demonstrated the feasibility of the biochars derived from woody biomass to be as a low-cost potential adsorbent for heavy metals as Cu(II) removal in aquatic system.

• Proceedings of the Korea Water Resources Association Conference
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• 2005.05b
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• pp.454-458
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• 2005

여름철 집중강우시 유입되는 고탁수층은 저수지의 밀도성층으로 인하여 표수층 하부에 위치하며, 이를 적기에 배제하지 않을 경우에는 수평방향의 확산현상과 연직방향의 전도현상으로 인해 저수지 전역에 분포하게 되어, 탁수현상의 장기화를 유발한다. 일본 사메우라댐의 경우에는 1977년 9월 태풍 17호에 의한 집중호우로 대량의 고탁수가 유입되어 하류하천으로의 댐 방류수의 고탁도현상이 장기간 지속된 바 있다. 이와 같은 탁수장기화에 대한 저수지내 대책의 하나로서 홍수유입후 탁도가 높은 물을 단기간동안 방류하고, 갈수기에는 탁도가 낮은 물을 방류하는 선택취수 개념이 제안된 바 있다. 국내의 임하댐의 경우에도 2002년 태풍 '루사'와 2003년 태풍 '매미'로 인해 발생한 탁수가 전도현상을 거치며 저수지내 전수층에 확산됨으로써 댐방류수의 고탁도현상이 장기화된바 있다. 이로 인하여 댐하류에 위치한 안동시 용상정수장의 동절기 정수처리 장애로 2003년 11월이후 약 160여일동안 댐방류가 중단된 바 있다. 임하댐의 경우에도 탁수장기화에 대한 대책으로서 홍수기 고탁수층을 우선배제한 후, 저수지내에 유입되는 청수을 담수함으로써 이를 방지할 수 있다. 이러한 선택취수 기법은 동일한 수리동역학적 개념을 적용하여 하류하천의 냉해문제를 대비하기 위한 방법으로 표층의 온수를 선택방류하는 데에도 이용될 수 있다. 이와 같은 선택취수를 효율적으로 수행하기 위해서는 취수시설에 접근하는 밀도흐름을 정확하게 이해하고 있어야 한다. 또한 취수탑 인근에서의 온도 및 탁도 등의 연직분포를 실시간으로 파악할 수 있는 자동수질측정시스템이 구축되어 있어야 한다. 현재 임하댐의 경우, 하류하천의 수질을 고려한 효율적인 탁수배제를 위하여 상류 유입부 2개소, 발전취수탑 전면 1개소, 조정지댐 1개소 등 총 4개의 자동수질측정시스템이 설치되어 탁도와 온도가 실시간으로 모니터링되고 있으며, 향후 강우시 저수지에서의 밀도흐름을 보다 정밀하게 모니터링하기 위하여 저수지내 4개의 주요지점에 추가적으로 설치될 예정이다. 이와 같은 자동측정시스템과 더불어 주기적인 세부 수질조사를 실시하면 저수지내의 온도성층 변화와 탁수층 분포를 매우 정확하게 파악할 수 있으며, 호소의 수질모형과 연계하여 강우시 탁수 도달시간과 탁수량을 사전에 예측할 수도 있다. 또한 이와 같은 기초적인 정보를 바탕으로 하류하천의 탁수 피해를 최소화할 수 있는 선택취수탑의 운영방안을 수립할 수 있다 본 연구에서는 이를 위해 선택취수탑 주위의 성층흐름을 기존의 실험자료와 수치해석을 통하여 분석하였고, 온도성층구조나 취수구의 위치변화에 따른 방류수 수질특성을 조사하였다.

• Korean Journal of Microbiology
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• v.47 no.1
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• pp.14-21
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• 2011

Riboflavin synthase catalyzes the formation of one molecule of each riboflavin and 5-amino-6-ribitylamino-2,4-pyrimidinedione by the transfer of a 4-carbon moiety between two molecules of the substrates, 6,7-dimetyl-8-ribityllumazine. The most remarkable feature is the sequence similarity between the N-terminal half (1-97) and the C-terminal half domain (99-213). To investigate the structure and fluorescent characteristics of the N-terminal half of riboflavin synthase (N-RS) in Escherichia coli, more than 10 mutant genes coding for the mutated N-terminal domain of riboflavin synthase were generated by polymerase chain reaction. The genes coding for the proteins were inserted into pQE vector designed for easy purification of protein by 6X-His tagging system, expressed, and the proteins were purified. Almost all mutated N-terminal domain of riboflavin synthases bind to 6,7-dimethyl-8-ribityllumazine and riboflavin as fluorescent ligands. However, N-RS C47D and N-RS ET66,67DQ mutant proteins show colorless, indicating that fluorescent ligands were dissociated during purification. In addition, most mutated proteins show low fluorescent intensity comparing to N-RS wild type, whereas N-RS C48S posses stronger fluorescent intensity than that of wild type protein. Based on this result, N-RS C48S can be used as the tool for high throughput screening system for searching for the compound with inhibitory effect for the riboflavin synthase.

• The Korean Journal of Food And Nutrition
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• v.23 no.4
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• pp.582-587
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• 2010

본 연구에서는 녹두 유전자원의 지방산 함량을 신속 대량 검정하는 기술을 개발하여 유전자원 활용 및 육종 촉진에 기여하고자 하였다. 유전자원 평가에 적합한 신속하고 비파괴적인 지방산 함량 평가기술을 개발하기 위해 공시자원 1,125점의 녹두 종자를 종실상태와 분쇄한 분말상태로 근적외선분광분석기(NIR)를 이용하여 1,104~2,494 nm에서의 스펙트럼을 얻고 이들 중 스펙트럼이 중복되지 않는 원산지가 다양한 대표자원 106점을 선발하여 일반적인 방법으로 지방산 함량을 분석하고, 이 값과 NIR 스펙트럼 흡광도값 간의 상관분석을 위한 calibration set로 활용하였다. 그 결과 palmitic acid, stearic acid, oleic acid, linoleic acid, linolenic acid 및 total fatty acid에 대한 NIR 흡광도와의 상관계수 $R^2$이 각각 0.74, 0.18, 0.12, 0.72, 0.48 및 0.78로 나타났고, 이들 중 $R^2$가 높은 검량식을 미지의 시료 10점으로 검증한 결과, palmitic, linoleic 및 total fatty acid에 대한 검증 상관계수 $R^2$이 0.96, 0.74, 0.81로 나타나, 다양한 녹두 유전자원의 지방산함량 신속 대량 예측에 유효하게 활용될 수 있는 것으로 나타났다. 한편, 공시된 녹두 유전자원 115점 중에서 자원번호 IT208075 자원은 저 지방산 자원($14.24\;mg\;g^{-1}$)으로 선발되었고, IT163279 자원은 고 지방산 자원($18.43\;mg\;g^{-1}$)으로 선발되어 향후 녹두작물의 성분육종에 유용할 것으로 생각된다.

• The Journal of Internal Korean Medicine
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• v.25 no.1
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• pp.1-15
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• 2004

Objective : This study was aimed to evaluate the anti-starvation stress effect of Liriopis Tuber on mice. Method : The first experiment was done to mice which have a high corticosterone level at a short term starvation. The plasma corticosterone level of each mouse was measured over time at 12, 24, 36, 48, 72 hours of starvation respectively. The second experiment was done in the two groups(LT A and LT B) of mice which were in famine for 36.5 hours after being administered Liriopis Tuber three times with different doses(LT A: 1.0 g/.kg and LT B: 3.0g/kg) for three days. The plasma corticosterone levels and rectal temperature were measured when a 36.5-hour of starvation period was finished. Results : 1. The corticosterone level in the mice was significantly increased after a 36.5-hour of starvation. (P<0.001). 2. The plasma corticosterone level was decreased significantly in the group of 3.0g/kg, compared to the control group.(P<0.05) 3. The rectal temperature in the groups of 1.0g/kg and 3.0g/kg were increased slightly in both group. But the two groups didn't show significant change. Conclusion: Based on the above results, Liriopis Tuber might have anti-starvation stress effect and it may be used as anti-starvation stress medicine and anti-stress medicine. Conclusion : Based on the above results, Liriopis Tuber might have anti-starvation stress effect and it may be used as anti-starvation stress medicine and anti-stress medicine.