• 상하수도학회지
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• 제18권6호
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• pp.748-757
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• 2004

In this study, we analyzed the sensitivity of parameters which affect the result of ILLUDAS model, in the various rainfall conditions. The three basins including Namgaja, Kings creek, Gray haven were selected for this research. The rainfall conditions are considered in terms of the rainfall frequency, the duration and the distribution. In most cases, the impermeability area ratio, the sewer slope, and the sewer roughness coefficient give more significant effects on the results than others. The results show that as increasing the rainfall frequency, the sensitivity of the parameters, sewer slope and roughness coefficient are rised, while the impermeability area ratio is decreasing. And also, for the duration of rainfall, the impermeability area ratio's sensitivity shows similar tendency. In case of the rainfall distribution, the parameters of the sewer roughness and the impermeability area ratio show more sensitive in Huff distribution. Especially, The impermeability area ratio is the most sensitive parameter in Central blocking and Yen & Chow distributions respectively.

• Biotechnology and Bioprocess Engineering:BBE
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• 제10권3호
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• pp.280-283
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• 2005

The adsorption of a model protein bovine serum albumin (BSA) in expanded bed chromatography was undertaken by exploiting a commercially available expanded bed column (20 mm i.d.) from UpFront Chromatography and Streamline DEAE $(\rho=1.2g/cm^3)$ from Amersham Pharmacia Biotechnology. The influence of whole yeast cells on the adsorption capacity of column was explored by employing yeast cells in a concentration ranged of 0 to $15\%(w/v)$. Equilibrium isotherms for adsorption of BSA on Streamline DEAE were correlated by using Langmuir equation. The presence of yeast cells resulted in decreased of BSA binding capacity in both batch binding and expanded bed chromatography. Results indicated that the yeast cells act as competitor for proteins to bind to the sites on adsorbents.

• Biotechnology and Bioprocess Engineering:BBE
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• 제10권6호
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• pp.552-555
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• 2005

The influence of whole yeast cells $(0{\sim}15%\;w/v)$ on the protein adsorption performance in dye-ligand chromatography was explored. The adsorption of a model protein, bovine serum albumin (BSA), was selected to demonstrate this approach. The UpFront adsorbent $(p=1.5\;g/cm^3)$ derivatised with Cibacron Blue 3GA and a commercially available expanded bed column (20 mm i.d.) from UpFront Chromatography, Denmark, were employed in the batch binding and expanded bed operation. The BSA binding capacity was demonstrated to not be adversely affected by the presence of yeast cells. The dynamic binding capacity of BSA at a $C/C_0=0.1$ biomass concentration of 5, 10, 15% w/v were 9, 8, and 7.5mg/mL of settled adsorbent, respectively.

• Biotechnology and Bioprocess Engineering:BBE
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• 제11권3호
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• pp.268-272
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• 2006

A dense, pellicular UpFront adsorbent ($p=1.5 g/cm^3$, UpFront Chromatography, Cophenhagen, Denmark) was characterized in terms of hydrodynamic properties and protein adsorption performance in expanded bed chromatography. Cibacron Blue 3GA was immobilised into the adsorbent and protein adsorption of bovine serum albumin (BSA) was selected to test the setup. The Bodenstein number and axial dispersion coefficient estimated for this dense pellicular adsorbent was 54 and $1.63{\times}10^{-5}m^2/s$, respectively, indicating a stable expanded bed. It could be shown that the BSA protein was captured by the adsorbent in the presence of 30% (w/v) of whole-yeast cells with an estimated dynamic binding capacity $(C/C_o = 0.01)$ of approximately 6.5 mg/mL adsorbent.

• Biotechnology and Bioprocess Engineering:BBE
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• 제11권5호
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• pp.466-469
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• 2006

The bed stability of Streamline DEAE (p = 1.2 g/mL) in a 20mm (i.d.) glass expanded bed contactor, and its performance on the recovery of glucose 6-phosphate dehydrogenase (G6PDH) from unclarified yeast homogenate were investigated. A residence time distribution study showed that a stable expanded bed was achieved. The theoretical plate and Bodenstein numbers determined were 25 and 53, respectively. A recovery yield of 87% and purification factor of 4.1 were achieved in the operation using 5% (w/v) biomass concentration feedstock. The performance of the anion exchange EBAC was still considerable good at a biomass concentration as high as 15% (w/v).

• 한국수자원학회:학술대회논문집
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• 한국수자원학회 2019년도 학술발표회
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• pp.321-321
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• 2019

수공구조물을 설계하는데 설계강우량을 결정하기 위한 강우시간분포의 생산은 중요한 요소이고 주로 Huff 방법, Mononobe 방법, SCS 방법, Yen and Chow 방법 등을 이용하고 있다. 그러나 Huff 방법 등들로 한국의 극치강우사상을 고려하기에는 반영하지 못하는 한계점이 있었다. 본 연구에서는 기존 방법들의 한계점을 극복하고 한국 극치강우사상의 집중지속시간 특성을 반영하는 HSD 방법을 제시하여 서울과 대구광역시에 적용해 설계빈도에 관한 강우 시간분포를 발생시키고 설계 강우량을 산정하며 기존 기법들과 비교하였다. 이에 따라 연구결과를 통해 Huff 방법과 HSD 방법의 첨두 강우량은 지속시간이 길어질수록 차이가 상당하였고 과거 극치강우사상을 살펴보면 HSD 방법을 반드시 고려할 필요가 있다고 판단되었다. 게다가 기후변화로 인해 집중호우현상이 더욱 심해져서 Huff 방법만 이용할 경우 추후에 큰 문제가 발생할 수도 있다. 그래서 수공 구조물 설계 시 HSD 방법을 이용한 대상 지역의 집중지속시간 특성을 반영하는 설계 강우량과 Huff 방법을 이용한 설계 강우량을 같이 고려하여 비교 선택하면 안전한 수공구조물 설계를 실시 할 수 있을 것으로 판단되었다.

• Biotechnology and Bioprocess Engineering:BBE
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• 제10권3호
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• pp.284-288
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• 2005

Baker's yeast was disrupted in a 1.4-L stainless steel horizontal bead mill under a continuous recycle mode using 0.3 mm diameter zirconia beads as abrasive. A single pass in continuous mode bead mill operation liberates half of the maximally released protein. The maximum total protein release can only be achieved after passaging the cells 5 times through the disruption chamber. The degree of cell disruption was increased with the increase in feeding rate, but the total protein release was highest at the middle range of feeding rate (45 L/h). The total protein release was increased with an increase in biomass concentration from 10 to $50\%$(w/v). However, higher heat dissipation as a result of high viscosity of concentrated biomass led to the denaturation of labile protein such as glucose 6-phosphate dehydrogenase (G6PDH). As a result the highest specific activity of G6PDH was achieved at biomass concentration of $20\%$(ww/v). Generally, the degree of cell disruption and total protein released were increased with an increase in impeller tip speed, but the specific activity of G6PDH was decreased substantially at higher impeller tip speed (14 m/s). Both the degree of cell disruption and total protein release increased, as the bead loading increased from 75 to $85\% (v/v)$. Hence, in order to obtain a higher yield of labile protein such as G6PDH, the yeast cell should not be disrupted at biomass concentration and impeller tip speed higher than $20\%(w/v)$ and 10 m/s, respectively.

• 한국농공학회논문집
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• 제48권7호
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• pp.3-14
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• 2006

The objective of this study was to develop a GIS-based decision support system (GIS-USLE system) to estimate soil erosion and evaluate its effect on concentrated upland plots in Godang district, Korea. This system was developed for the ArcView environment using A VENUE script. Three modules were used in the GIS-USLE system, namely pre-processing, the USLE factors calculator module, and post-processing. This system benefits from a user friendly environment that allows users with limited computer knowledge to use it. This system was applied to 1,285 individual upland plots ranging from 0.005 to 1.347 ha in size with an average slope steepness of 14 %. The rainfall distributions were estimated using the three methods, namely Mononobe and Yen-Chow with Triangle and with Trapezoid type, and then used to calculate the rainfall erosivity factor. The soil erosion amounts from the 1,285 individual plots in the study area by 2 year return period with a 24h maximum rainfall amount of 154.6 mm were estimated at 5 tons/ha on average. Slope appeared to be the most important factor affecting soil erosion estimation, as expected. The prototype model was applied to the project area, and the results appeared to support the practical applications. By examining many fields simultaneously, this system can easily provide fast estimation of soil erosion and thus reveal the spatial pattern of erosion from fields in a region. This study will help estimate and evaluate soil erosion in concentrated upland districts and identify the best management practices.

• 물과 미래
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• 제29권6호
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• pp.189-201
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• 1996

본 연구는 우리나라에 적합한 강우의 시간 분포모형을 산정하고, 지역의 지형학적 특성을 고려한 강우-유출 모형인 GIUH 모형을 제시함으로써 설계자가 가장 적절하게 실무에 적용하도록 도움을 주는데 있다. 연구결과, 우리나라의 강우는 삼각형 분포의 적용성이 가장 높았으며 강우-유출 모형중에서는 지형학적 순간단위도(GIUH) 모형의 우수성이 입증되었다. 분석을 위한 기초자료는 IHP 대표시험유역의 12년간 13,000개 강우사상과 73개의 강우-유출 관측자료를 이용하였으며, 이 자료들을 이용하여 유역면적별 5가지 강우의 시간 분포모형을 산정, 실무에 적용할 수 있도록 제시하고 이를 GIUH로서 적용성을 검증하였다. 또한, 우리나라 실무에 주로 적용되는 4가지 강우-유출모형인 합리식, Kajiyama 공식, Nakayasu 합성단위도와 Clark 순간단위도와 본 연구의 GIHU 를 통하여 유역면적별 각 강우-유출 모형의 매개변수, 적용한계 및 비유량 함수식을 제시하여 미계측유역의 설계홍수량 산정의 기준을 마련하고자 한다.

• 한국수자원학회:학술대회논문집
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• 한국수자원학회 2004년도 학술발표회
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• pp.681-686
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• 2004

수공구조물의 설계를 위해서는 해당 수공구조물의 중요도에 따른 설계빈도 및 설계유량과 설계수위 등의 설정이라는 과정이 필요하다. 설계빈도는 하천설계기준에 제시되어 있는 바와 같이 시설물의 입지조건과 중요도에 따라 기준이 제시되어 있으며 설계홍수량은 확률강우량을 기초로 한 설계강우를 결정하고, 결정된 설계우량에 의한 유출량의 산정작업이 필요하다. 이와 같은 설계수량의 산정에 있어서 설계강우의 지속기간 설정은 매우 중요한 작업이다. 일반적으로 동일한 설계빈도의 홍수량은 지속기간에 따라 많은 차이를 보이고 있다. 따라서 설계강우의 지속시간 설정은 매우 중요한 설계인자가 되므로 본 연구에서는 IHP유역인 위천유역을 대상으로 최근 권장되고 있는 설계강우의 지속시간 선정을 위한 개념인 임계지속기간을 산정하여 임계지속기간에 영향을 미치는 수문인자들에 대해 살펴보고자 한다. 본 연구에서는 IHP 유역인 위천 유역(동곡 외 4개 소유역)을 대상으로 설계홍수량의 첨두유출량이 최대로 발생하는 강우지속기간을 임계지속기간으로 설정하였으며, 설계홍수량의 산정시 설계강우로부터 홍수량을 산정하기 위한 일련의 절차에서 이용되는 각종 수문요소들, 즉 강우시간분포와 유효우량 산정방법, 유출모형 그리고 면적의 변화에 따른 임계지속기간의 변화를 연구하였다. 본 연구에서는 임계지속기간의 개념을 고려할 설계강우의 지속기간을 산정하기 위해 필요한 각 수문요소별 산정방법은 국내 자료로부터 제안된 방법을 우선 사용하였으며, 임계지속기간의 개념에 따른 설계강우의 지속기간 산정을 위해 확률강우량 산정, 강우의 시간분포(Huff 분포, Yen & Chow의 삼각형 분포), 유효우량 산정방법(AMC-II, AMC-III, CN37), 대표단위도와 6가지 합성단위도법을 적용하였다. 산정 된 결과로부터 임계지속기간 산정에 영향을 주는 각 수문인자 중 강우시간분포와 유효우량 산정방법 그리고 유출모형에 대해 자자 검토하였으며, 최종적으로 면적에 따른 임계지속기간과 유출량의 변화를 검토해 보았다.