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The Performance of Anion Exchange Expanded Bed Adsorption Chromatography on the Recovery of G6PDH from Unclarified Feedstock with High Biomass Concentration  

Chow, Yen Mei (Department of Process and Food Engineering, Faculty of Engineering, Universiti Putra Malaysia)
Tey, Beng Ti (Department of Chemical and Environmental Engineering, Faculty of Engineering, Universiti Putra Malaysia)
Ibrahim, Mohd Nordin (Department of Process and Food Engineering, Faculty of Engineering, Universiti Putra Malaysia)
Ariff, Arbakariya (Department of Bioprocess Technology, Faculty of Biotechnology and Molecular Science, Universiti Putra Malaysia)
Ling, Tau Chuan (Department of Process and Food Engineering, Faculty of Engineering, Universiti Putra Malaysia)
Publication Information
Biotechnology and Bioprocess Engineering:BBE / v.11, no.5, 2006 , pp. 466-469 More about this Journal
Abstract
The bed stability of Streamline DEAE (p = 1.2 g/mL) in a 20mm (i.d.) glass expanded bed contactor, and its performance on the recovery of glucose 6-phosphate dehydrogenase (G6PDH) from unclarified yeast homogenate were investigated. A residence time distribution study showed that a stable expanded bed was achieved. The theoretical plate and Bodenstein numbers determined were 25 and 53, respectively. A recovery yield of 87% and purification factor of 4.1 were achieved in the operation using 5% (w/v) biomass concentration feedstock. The performance of the anion exchange EBAC was still considerable good at a biomass concentration as high as 15% (w/v).
Keywords
expanded bed adsorption; G6PDH; biomass; anion exchange; yeast;
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Times Cited By KSCI : 1  (Citation Analysis)
Times Cited By Web Of Science : 1  (Related Records In Web of Science)
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1 Bonnerjea, J., S. Oh, M. Hoare, and P. Dunnill (1986) Protein purification: The right step at the right time. Bio/Technol. 4: 954-958   DOI
2 Thommes, J., M. Halfar, S. Lenz, and M.-R. Kula (1995) Purification of monoclonal antibodies from whole hybri-doma fermentation broth by fluidized bed adsorption. Biotechnol. Bioeng. 45: 205-211   DOI   ScienceOn
3 Boschetti, E., L. Guerrier, P. Girot, and J. Horvath (1995) Preparative high-performance liquid chromatographic separation of proteins with HyperD ion-exchange supports. J. Chromatogr. B, Biomed. Appl. 664: 225-231   DOI   ScienceOn
4 Bradford, M. M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 72: 248-254   DOI   ScienceOn
5 Tan, Y. P, T. C. Ling, W. S. Tan, K. Yusoff, and B. T. Tey (2005) Purification of recombinant nucleocapsid protein of Newcastle disease virus from unclarified feedstock using expanded bed adsorption chromatography. Protein Expr. Purif. 46: 114-121   DOI   ScienceOn
6 Fernandez-Lahore, H. M., R. Kleef, M. R. Kula, and J. Thommes (1999) The influence of complex biological feedstock on the fluidization and bed stability in expanded bed adsorption. Biotechnol. Bioeng. 64: 484-496   DOI   ScienceOn
7 Lu, M.-H., D.-Q. Lin, Y.-C. Wu, J.-X. Yun, L.-H. Mei, and S.-J. Yao (2005) Separation of Nattokinase from Bacillus subtilis fermentation broth by expanded bed adsorption with mixed-mode adsorbent. Biotechnol. Bioprocess Eng. 10: 128-135   과학기술학회마을   DOI   ScienceOn
8 Ling, T. C., C. K. Loong, W. S. Tan, B. T. Tey, W. M. W. Abdullah, and A. Ariff (2004) Purification of filamentous bacteriophage M13 by expanded bed anion exchange chromatography. J. Microbiol. 42: 228-232
9 Thommes, J., M. Weiher, A. Karau, and M.-R. Kula (1995) Hydrodynamics and performance in fluidized bed adsorption. Biotechnol. Bioeng. 48: 367-374   DOI   ScienceOn
10 Bruce, L. J. and H. A. Chase (2001) Hydrodynamics and adsorption behaviour within an expanded bed adsorption column studied using in-bed sampling. Chem. Eng. Sci. 56:3149-3162   DOI   ScienceOn
11 Chow, Y. M., B. T. Tey, M. N. Ibrahim, A. Ariff, and T C. Ling (2005) The disruption of Saccharomyces cerevisiae cells and release of glucose 6-phosphate dehydrogenase (G6PDH) in a horizontal dyno bead mill operated in continuous recycling mode. Biotechnol. Bioprocess Eng. 10: 284-288   과학기술학회마을   DOI   ScienceOn
12 Ameskamp, N., C. Priesner, J. Lehmann, and D. Lutke-meyer (1999) Pilot scale recovery of monoclonal antibodies by expanded bed ion exchange adsorption. Bioseparation 8: 169-188   DOI   ScienceOn
13 Viloria-Cols, M. E., R. Hatti-Kaul, and B. Mattiasson (2004) Agarose-coated anion exchanger prevents cell-adsorbent interactions. J. Chromatogr. A 1043: 195-200   DOI   ScienceOn
14 Chow, Y. M., B. T. Tey, M. N. Ibrahim, A. Ariff, and T. C. Ling (2006) Protein adsorption and hydrodynamic stability of a dense, pellicular adsorbent in high-biomass expanded bed chromatography. Biotechnol. Bioprocess Eng. 11: 268-272   과학기술학회마을   DOI   ScienceOn
15 Thommes, J., A. Bader, M. Halfar, A. Karau, M. R. Kula (1996) Isolation of monoclonal antibodies from cell containing hybridoma broth using a protein A coated adsorbent in expanded beds. J. Chromatogr. A 752: 111-122   DOI   ScienceOn
16 Nayak, D. P., S. Ponrathnam, and C. R. Rajan (2001) Macroporous copolymer matrix. IV Expanded bed adsorption application. J. Chromatogr. A 922: 63-76   DOI   ScienceOn
17 Chow, Y. M., B. T. Tey, M. N. Ibrahim, A. Ariff, and T. C. Ling (2005) The influence of bakers' yeast cells on protein adsorption in anion exchange expanded bed chromatography. Biotechnol. Bioprocess Eng. 10: 280-283   과학기술학회마을   DOI   ScienceOn
18 Chow, Y. M., B. T. Tey, M. N. Ibrahim, A. Ariff, and T. C. Ling (2005) The influence of bakers' yeast cells on protein adsorption performance in dye-ligand expanded bed chromatography. Biotechnol. Bioprocess Eng. 10: 552-555   과학기술학회마을   DOI   ScienceOn
19 Barnfield Frej, A.-K., H. J. Johansson, S. Johansson, and P. Leijon (1997) Expanded bed adsorption at production scale: Scale-up verification, process example and sanitiza-tion of column and adsorbent. Bioprocess Biosyst. Eng. 16: 57-63