• 대한예방한의학회지
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• 제16권1호
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• pp.43-56
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• 2012

1. 추출방법, 추출농도에 따라 항균력의 차이가 있었으나, 일반양파, 자색양파의 1%, 5%, 10% 생즙추출물 모두, 피부상재균인 Staphylococcus epidermidis(KCTC1917), Staphylococcus aureus subsp(ATCC6538)에 양호한 항균력을 보였다(81%~100%). 어성초 열수추출물은 10% 고농도에서만 피부상재균인 Staphylococcus epidermidis(KCTC1917), Staphylococcus aureus subsp(ATCC6538)에 항균력을 보였다(100%). 2. 자색양파, 어성초, 일반양파의 1%, 5%, 10% 추출물 모두, 식중독 균인 Escherichia coil, Salmonella enterica subsp. enterica에서는 항균력을 보이지 않았다. 3. 자색양파 추출물과 어성초 추출물을 0.01%, 0.05%, 0.1%, 0.5%로 처리하였을 때, 피부장벽 조절인자인 filaggrin과 SPT의 발현을 농도 의존적으로 증가시켰다. 반면, 일반양파 추출물도 농도 의존적으로 filaggrin과 SPT발현양을 증가시켰지만, 자색양파 추출물에 비하여 발현양이 많지 않았다. 4. 피부 염증 반응 인자에 미치는 영향을 알아보기 위해서 COX-2의 발현과 AP-1의 발현을 분석한 결과, 자색양파와 어성초의 0.01%, 0.05%, 0.1%, 0.5% 추출물에서 농도 의존적으로 COX-2의 발현과 AP-1의 발현이 감소하였다. 반면 일반양파 추출물도 농도 의존적으로 COX-2의 발현과 AP-1의 발현이 감소하였으나, 자색 양파 추출물에 비하여 감소양이 적었다. 5. 자색양파 추출물과 어성초 추출물은 피부 장벽기능 조절인자인 filaggrin과 SPT의 발현양을 증가시켜 줌으로써, 피부의 수분 유지 기능을 강화하였고, 농도 의존적으로 피부 염증 반응 인자도 감소시켜 항염증효과가 있어, 피부미용기능재료로서의 가능성 보여주었다.

• 생명과학회지
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• 제14권6호
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• pp.925-930
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• 2004

양파 열수 추출물의 항산화능 및 인공소화 전과후의 항돌연변이 효과를 BHT와 ascorbic acid와 비교하여 조사하였다. 총 페놀 및 플라보노이드의 함량은 노란색 양파 추출물이 횐색 양파 추출물보다 많았으며, 양파 추출물의 과산화수소 소거능은 농도가 증가함에 따라 비례적으로 증가하였다. $\beta-carotene-linoleate\;system$에서 항산화능과 reducing power는 증가되었으나, BHT와 ascorbic acid보다는 낮았다. 양파열수 추출물의 인공소화 후에 IQ와 MNNG로 처리된 Salmonella typhimurium TA80과 TA100에 대하여 항돌연변이 효과가 나타났으며, 노란색 양파가 횐색 양파보다 높게 나타났다. 결론적으로 양파 추출물을 소화시키면 항산화 및 항돌연변이의 효과는 양파의 페놀과 플라보노이드의 성분 변화에 의한 것으로 판단된다.

• 한국의류학회지
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• 제29권6호
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• pp.868-876
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• 2005

Silk fabrics were dyed with yellow natural materials. Gardenia, turmeric, amur cork tree, safflower, Japanese pagoda tree, and onion were used as natural materials. Color image of natural dyed silk fabrics was classified by 4 factors(Cheerfulness, Comfortness, Pastrol, Revealation). Cheerfulness factor affected significantly with color image. Amur cork tree or turmeric dyed fabrics were shown as light and cute, safflower or gardenia dyed fabric shown as comfort, and onion dyed fabrics shown as mature and simple. Color image with specialty was significant difference in Preference and Revelation factor. Preference was appeared as amur cork tree>turmeric>gardenia>safflower>Japanese pagoda tree>onion.

• Food Science and Biotechnology
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• 제18권1호
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• pp.108-112
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• 2009

Antioxidant and anticancer effects of methanolic extracts from the flesh (WFME) and peel (WPME) of white onion, the flesh (YFME) and peel (YPME) of yellow onion, the flesh (RFME) and peel (RPME) of red onion were studied. The content of total phenolics in WFME, WPME, YPME, YFME, RPME, and RFME were $0.260{\pm}0.01$, $4.480{\pm}0.23$, $0.319{\pm}0.02$, $719.12{\pm}37.36$, $0.248{\pm}0.01$, and $806.21{\pm}26.38\;mg/g$, respectively. The quercetin content of WFME, WPME, YFME, YPME, RFME, and RPME were $12.56{\pm}0.19$, $3.57{\pm}0.14$, $15.24{\pm}0.65$, $755.29{\pm}22.24$, $5.70{\pm}0.23$, and $774.03{\pm}29.48\;mg$/100 g, respectively. Like total phenolics, the highest 2,2'-diphenyl-1-picrylhydrazyl (DPPH) scavenging activities were found in RPME. However, inhibitory effects on lipid oxidation of RPME were similar to those of WPME and YPME. In addition, inhibitory effect of WPME, YPME, and RPME for human breast cancer cell (MCF-7) growth were 78.43, 81.90, and 96.52% while those on human prostate cancer cell (LNcap) were 71.58, 77.93, and 98.47% at $100{\mu}g/mL$, respectively. Total phenolics, quercetin content, antioxidant, and anticancer activities exhibited significant variation among the 3 onion varieties in this experiment. Therefore, it is assumed that antioxidant and anticancer activities were affected by the total phenolics and quercetin level of onion.

• 한국의류산업학회지
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• 제22권5호
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• pp.561-569
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• 2020

In this study, the dyeing of Chrysanthemum indicum Linne alone and the combined dyeing of Chrysanthemum indicum Linne and onion shell were first performed to compare dyeing properties. A combination extract of Chrysanthemum indicum Linne and onion shell can be expected to improve coloration. A Hanbok design for children used a natural dyeing fashion dyeing method to make the Chrysanthemum pattern. Chrysanthemum indicum Linne is a type of wild chrysanthemum with various medicinal effects for headache and stress relief, skin diseases, insomnia and depression. Widely used onion shells have medicinal properties that help prevent and treat various adult diseases. The colors of silk dyed exclusively by Chrysanthemum indicum Linne, and the colors of silk dyed by Chrysanthemum indicum Linne and onion shell extract are yellow; in addition, silk dyed by Chrysanthemum indicum Linne and onion shell extracts were almost all unchanged. Four hanboks, which are comfortable for children to wear and use, were produced using Chrysanthemum indicum Linne and onion shell extracts. If children wear hanbok fashion products with auspicious patterns, they can provide a chance to simultaneously experience the traditional symbolism of Korean patterns and culture. In addition, it is expected to develop pride in traditional dress culture.

• 한국식품저장유통학회지
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• 제21권1호
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• pp.46-54
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• 2014

양파를 첨가한 청국장 제조 가능성을 평가하고, 제조된 시료들을 대상으로 항산화능을 비교하였다. 먼저 양파의 적정 첨가량을 판단하기 위해 노랑콩과 검정콩 각각의 중량을 기준으로 0, 5, 10, 20, 30%가 되도록 양파를 첨가하여 청국장을 제조한 후 관능평가를 실시하였다. 그 결과, 노랑콩과 검정콩 청국장 모두 20% 양파 첨가 청국장이 높은 기호도를 나타냈다. 그래서 20%의 양파를 첨가하여 제조한 청국장을 대상으로 특정성분의 함량 및 항산화능을 비교 평가하였다. 그 결과, 양파를 첨가하여 제조한 청국장이 첨가하지 않은 청국장보다 유의하게 더 높은 총 페놀성 및 flavonoid 함량을 나타냈다. 그리고 DPPH radical-scavenging 활성평가에 있어서도 양파를 첨가하여 제조한 청국장이 첨가하지 않고 제조한 청국장보다 더 높은 radical-scavenging 활성을 나타냈다. 또 쥐 혈장 산화에 따른 CE-OOH 생성 억제능 평가에 있어서도 양파 첨가 청국장 추출물을 투여한 쥐혈장이 양파를 첨가하지 않은 청국장 추출물을 투여한 쥐 혈장에 비해 더 높은 산화 억제능을 나타냈다. 이상의 결과들은 양파에 다량 함유된 quercetin을 포함한 flavonoid들과 발효과정 중 생성되는 다양한 페놀성 화합물들에 의한 효과라 판단된다. 본 연구결과가 국산 양파의 가공시장확대 및 청국장 소비의 저변확대를 위한 기초자료로 활용되길 기대한다.

• The Plant Pathology Journal
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• 제40권1호
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• pp.40-47
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• 2024

Garlic can be infected by a variety of viruses, but mixed infections with leek yellow stripe virus, onion yellow dwarf virus, and allexiviruses are the most damaging, so an easy, inexpensive on-site method to simultaneously detect at least these three viruses with a certain degree of accuracy is needed to produce virus-free plants. The most common laboratory method for diagnosis is multiplex reverse transcription polymerase chain reaction (RT-PCR). However, allexiviruses are highly diverse even within the same species, making it difficult to design universal PCR primers for all garlic-growing regions in the world. To solve this problem, we developed an inexpensive on-site detection system for the three garlic viruses that uses a commercial mobile PCR device and a compact electrophoresis system with a blue light. In this system, virus-specific bands generated by electrophoresis can be identified by eye in real time because the PCR products are labeled with a fluorescent dye, FITC. Because the electrophoresis step might eventually be replaced with a lateral flow assay (LFA), we also demonstrated that a uniplex LFA can be used for virus detection; however, multiplexing and a significant cost reduction are needed before it can be used for on-site detection.

• 한국의류산업학회지
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• 제9권4호
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• pp.441-444
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• 2007

For the purpose of application to new natural dyestuff, the dyeability of Purple onion shell extract was analyzed. It was dyed in cotton fabric according to various dye weight, dyeing temperature, dyeing time. And the effects of mordanting conditions were examined as color differences and color fastnesses. The dyeaffinities were increased as were increased dye weight, especially 100% owf. The optimum dyeing condition of Purple onion shell extract was 40minutes at $60^{\circ}C$. The dyeaffinity was increased at pre-mordanted condition, and color difference was increased distinctly at post mordanted condition. The hue of all mordanted cotton fabrics changed into Yellow where as non mordanted cotton fabric was Red. The color fastnesses of mordanted cotton fabrics were generally not so god, but light fastness was improved in post-Cu mordanted fabric.

• 원예과학기술지
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• 제31권1호
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• pp.72-79
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• 2013

Inactivation of the gene coding for dihydroflavonol 4-reductase (DFR) is responsible for the color difference between red and yellow onions (Allium cepa L.). Two inactive DFR-A alleles, DFR-$A^{PS}$ and DFR-$A^{DEL}$, were identified in our previous study. A functional marker was developed on the basis of the premature stop codon that inactivated the DFR-$A^{PS}$ allele. A derived cleaved amplified polymorphic sequences (dCAPS) primer was designed to detect the single nucleotide polymorphism, an A/T transition, which produced the premature stop codon. Digested PCR products clearly distinguished the homozygous and heterozygous red $F_2$ individuals. Meanwhile, to develop a molecular marker for detection of the DFR-$A^{DEL}$ allele in which entire DFR-A gene was deleted, genome walking was performed and approximately 3 kb 5' and 3' flanking sequences of the DFR-$A^R$ coding region were obtained. PCR amplification using multiple primers binding to the extended flanking regions showed that more of the extended region of the DFR-A gene was deleted in the DFR-$A^{DEL}$ allele. A dominant simple PCR marker was developed to identify the DFR-$A^{DEL}$ allele using the dissimilar 3' flanking sequences of the DFR-A gene and homologous DFR-B pseudogene. Distribution of the DFR-$A^{PS}$ and DFR-$A^{DEL}$ alleles in yellow onion cultivars bred in Korea and Japan was surveyed using molecular makers developed in this study. Results showed predominant existence of the DFR-$A^{PS}$ allele in yellow onion cultivars.

• 한국가정과학회지
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• 제3권1호
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• pp.51-63
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• 2000

The purpose of this study was to identify the best dyeing conditions using onion's outer shell. and to apply to the method in practical daily life. To do this. we extracted quercetin from onion's outer shell and dyed several natural fabrics such as cotton, slack mercerized cotton, ramie. and silk. under the different conditions. Dyed fabrics were Investigated in the aspect of dyeability and colorfastness. The effective conditions for the light-fastness and washing-fastness also have been studied. The results of the experiment were varied with such conditions as temperature. time. pH degree. and treatment and types of mordants. The results are as follows ; 1. Fabrics dyed with onion's outer shell showed excellent dyeability even though there were no mordants, and the silk fabric dyed better than both cotton and ramie fabric. Furthermore, in the cases of repeated dyeing and treatment of mordants using AIK(SO$_4$)$_2$.12$H_2O$ and CuSO$_4$,.5$H_2O$ dyeability of specimen had been improved 2. Cellulose fabrics such as cotton, mercerized cotton and ramie showed the best dyeability under relatively low temperature in the range of 20~4$0^{\circ}C$. On the contrary to cellulose fabric, silk fabric showed the best dyeability under higher dyeing temperature. All fabrics had the higest K/S value at pH 3 regardless of the kind of fiber 3. Dyeing colors varied with the kind of mordants. Colors were turned into yellow in AIK(SO$_4$)$_2$.12$H_2O$ into Yellow-red in CuSO$_4$,.5$H_2O$ , and into green-Yellow in FeSO$_4$.7$H_2O$. As mordants, AIK(SO$_4$)$_2$.12$H_2O$, CuSO$_4$,. 5$H_2O$. FeSO$_4$.7$H_2O$. gallic acid and tartaric acid were used and especially AIK(SO$_4$)$_2$.12$H_2O$ showed the best dyeability and colorfastness in repeated dyeing. Mordants such as AIK(SO$_4$)$_2$.12$H_2O$ made fabrics have better chroma and washing-fastness though the light-fastness was poorer than non mordanting. 4. Repeated dyeing brought us deep color. When fresh dyebath was used each time, the dyeability was increased as the experiment was repeated more. When dyed with used dyebath several times, improved dyeability could not be expected. The optimal using times of the used dyebath was twice.