• Korean Journal of Microbiology
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• v.45 no.3
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• pp.251-256
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• 2009

Leaf samples and bulbs showing characteristic symptoms of virus infection were collected from Gang-won, Chung-nam, and Jeju Province of Korea in 2008-2009. Three viruses, Lily symptomless virus (LSV), Lily mottle virus (LMoV), and Cucumber mosaic virus (CMV) were detected by RT-PCR. Virus-infected plant samples were identified; 12 plants with LSV, 20 plants with LMoV, and 1 plant with CMV. Of the twelve LSV infected samples, seven samples were found to be mix-infected with LMoV and LSV. Symptoms of LMoV and LSV mixed infection were fairly severe, like as vein clearing, leaf curling, leaf mottling, leaf mosaic, and yellow streaking. Mixed infection with LMoV and LSV was also found in lily bulbs which have been stored under unfavorable environmental conditions. LMoV predominated in our tests, whereas spread of Lilyvirus X (LVX) was not found. The nucleotide sequences of coat protein (CP) region of seven isolates (4 LMoV, 2 LSV, and 1 CMV) were compared with the corresponding regions of LMoV (AJ564636), LSV (AJ516059) and CMV(AJ296154). The nucleotide sequence homologies between reference viruses and seven isolates were 95-99%. Complete sequencing of seven isolates is necessary to obtain more information on the molecular characteristics of these viruses as well as to increase sensitivity and rapidity of viral detection.

• Korean Journal of Microbiology
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• v.52 no.3
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• pp.344-351
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• 2016

A marine bacterium, designated as strain 50C-3, was isolated from a seawater sample collected from the East Sea of South Korea. The strain is a Gram-negative, aerobic, yellow colored polar-flagellated bacterium that grows at $20-50^{\circ}C$ and pH 5.5-8.5. Optimal growth occurred at $40-50^{\circ}C$, at pH 6.5-7.5, and in the presence of 2% (w/v) NaCl. Based on 16S rRNA gene sequence similarity, the isolate was considered to represent a member of the genus Ruegeria. The result of this analysis showed that strain 50C-3 shared 99.4% and 96.98% sequence similarity with Ruegeria intermedia CC-GIMAT-$2^T$ and Ruegeria lacuscaerulensis ITI-$1157^T$, respectively. Furthermore, strain 50C-3 showed clear differences from related strains in terms of several characteristics such as motility, carbon utilization, enzyme production, etc. The DNA G+C content was 66.7 mol%. Chemotaxonomic analysis indicated ubiquinone-10 (Q-10) as the predominant respiratory quinone. Based on phenotypic, chemotaxonomic, and phylogenetic characteristics, the isolate represents a novel variant of the Ruegeria intermedia CC-GIMAT-$2^T$, for which we named Ruegeria sp. 50C-3 (KCTC23890=DSM25519). Strain 50C-3 did not produce cellulase and agarase, but produced alkaline phosphatase, ${\alpha}$-galactosidase, and ${\beta}$-galactosidase. The three enzymes showed stable activities even at $50^{\circ}C$ and thus regarded as thermostable enzymes. Especially, the ${\beta}$-galactosidase activity enhanced by 1.9 times at $50^{\circ}C$ than that at $37^{\circ}C$, which may be very useful for industrial application.

• Journal of Conservation Science
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• v.25 no.3
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• pp.283-291
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• 2009

Methyl Bromide that was used as fumigation gas turned out to be the substance of destroying the ozone layer. For that reason, at the Montreal Protocol in 1987 the use of methyl bromide was forbidden starting 2005 in the advanced country. Also according to the 2007 Bali Protocolly methyl bromide is expected to be forbidden and therefore the purpose of this study is to find out the effects of substitution fumigation gas (Ethylene Oxide+HFC 134a, Methyl Iodide, Cyanogen and Argon) on the metal(silver, copper and iron), wood(oregon pine), pigment(yellow, red, blue, white and black), textile(hemp, ramie, jute, silk1 and silk2 / indigo, safflower and cork) and paper. After the fumigation test, ethylene oxide+HFC 134a did not have changes in the weight and color of the material itself before and after the experiment. On exterior alteration, color change occurred partly on paper and metal. Also, in most materials color change extent was 0.5 to 1.5 on the average and showed scanty difference. The materials after the fumigation test with methyl iodide did not show weight changes after the test. However, color changes more than 1.0 was shown in most of the materials especially in dyed textile material. In blue pigment, the discoloration on the surface could be seen by naked eyes. Fumigation test with cyanogen gas did not show weight changes and discoloration is more than 1.5 before and after the test. The weight changes of test materials with the argon gas was decreased about 3 to 6%. It can be observed that discoloration on paper was generated. Color changes can be seen on jute dyed with safflower and cork for two weeks with argon gas and the extent was 6.3 and 6.0.

• Economic and Environmental Geology
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• v.53 no.4
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• pp.383-395
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• 2020

The stratigraphical position of the Haengmae Formation can provide clues towards solving the hot issue on the Silurian formation, also known as Hoedongri Formation. Since the 2010s, there have been several reports denying the Haengmae Formation as a lithostratigraphic unit. This study aimed to clarify the lithostratigraphic and chronostratigraphic significance of the Haengmae Formation. The distribution and structural geometry of the Haengmae Formation were studied through geologic mapping, and the correlation of relative geologic age and the absolute age was performed through conodont biostratigraphy and zircon U-Pb dating respectively. The representative rock of the Haengmae Formation is massive and yellow-yellowish brown pebble-bearing carbonate rocks with a granular texture similar to sandstone. Its surface is rough with a considerable amount of pores. By studying the mineral composition, contents, and microstructure of the rocks, they have been classified as pebble-bearing clastic rocks composed of dolomite pebbles and matrix. They chiefly comprise of euhedral or subhedral dolomite, and rounded, well-sorted fine-grained quartz, which are continuously distributed in the study area from Biryong-dong to Pyeongan-ri. Bedding attitude and the thickness of the Haengmae Formation are similar to that of the Hoedongri Formation in the north-eastern area (Biryong-dong to Haengmae-dong). The dip-direction attitudes were maintained 340°/15° from Biryong-dong to Haengmae-dong with a thickness of ca. 200 m. However, around the southwest of the studied area, the attitude is suddenly changed and the stratigraphic sequence is in disorder because of fold and thrust. Consequently, the formation is exposed to a wide low-relief area of 1.5 km × 2.5 km. Zircon U-Pb age dating results ranged from 470 to 449 Ma, which indicates that the Haengmae Formation formed during the Upper Ordovician or later. The pebble-bearing carbonate rock consisted of clastic sediments, suggesting that the Middle Ordovician conodonts from the Haengmae Formation must be reworked. Therefore, the above-stated evidence supports that the geologic age of the Haengmae Formation should be Upper Ordovician or later. This study revealed that the Haengmae Formation is neither shear zone, nor an upper part of the Jeongseon Limestone, and is also not the same age as the Jeongseon Limestone. Furthermore, it was confirmed that the Haengmae Formation should be considered a unit of lithostratigraphy in accordance with the stratigraphic guide of the International Commission on Stratigraphy (ICS).

• Korean Journal of Environmental Biology
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• v.27 no.2
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• pp.183-190
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• 2009

Characterization of the analysis of forest vegetation, soil environmental conditions and water quality were performed from March 2003 to March 2007. The two basins were characterized by cultivated area (Kaesim reservoir) and mountain area (Jangchan reservoir), and divided into eleven small basins, where dynamics of pollutants, forest vegetation and soil environmental conditions were surveyed. The vegetation can be divided into 10 types by $Z\ddot{u}rich$-Montpellier school's method. Pearson coefficients between vegetation type and water quality were correlated with dissolved oxygen (DO) in the Quercus variabilis community at the 5% level and total phosphorus (TP) in the Larix leptolepis plantation at the 1% level. Especially total phosphorous and total nitrogen increased in small basins where the proportion of cultivated and residential area increased. The analysis of influences of pollutant discharge on water quality showed that pollutant charge was very low in forest land area ($Y_{T-P}$=-0.0017X+0.2215, r=0.16, $Y_{COD}$=- 0.0395X+8.5051 r=0.47). The soil types of western area were comparatively simple, but those of eastern area were complicated with regosols, red-yellow soils, lithosoles, etc. The pH, total solid (TS) and volatile substance (VS) of the forest and agricultural land soils collected in each site were 5.4~6.9, 75.8~80.2%, and 3.80%~5.80%, respectively. According to the analytical result of soil environmental conditions, heavy metal contents fell short to the mean value of natural conditions. Runoff amount (Y) and depth of topsoil (X) were negatively correlated, $Y_{ron}=-1.0088X_{top}+35.378$ (r=0.68). The correlation was much lower in up-stream but much higher in down-stream, because permeation into soil particle was larger on down-stream due to its more or less gentle slope. Pearson coefficients between soil pH and water pH were statistically significant at 1% level.

• Horticultural Science & Technology
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• v.28 no.6
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• pp.921-927
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• 2010

The objective of this study was to improve the breeding efficiency and consumption of red onion by analysis of chemical components. The red cultivar S&P5294 ($52.9mg{\cdot}g^{-1}$ F.W.) resulted in the highest sugar content, and sweetness whereas 'Redprime' had the lowest. For the pyruvic acid content the cultivar 'Redsun' showed the highest value, followed by 'Sunpower' (yellow onion) and S&P7270. Breeding lines had higher sugar content and sweetness than 'Redprime' and 'Sunpower', while they had less pyruvic acid value than 'Redsun' and 'Sunpower'. The the same trend was verified for the total phenol and total flavonoid, of which breeding lines were found to have more content than 'Redprime' or 'Sunpower'. Three major flavonoids, rutin (quercetin glycoside), myricetin, and quercetin, were identified and quantified whereas kaempferol was not detected in all cultivars. Rutin was the major flavonoid present in all cultivars (136.9 to $169.9{\mu}g{\cdot}g^{-1}$ F.W.), followed by quercetin (62.5 to $135.7{\mu}g{\cdot}g^{-1}$ F.W.). Relatively smaller amount of myricetin (11.2 to $16.1{\mu}g{\cdot}g^{-1}$ F.W.) was also detected. Among nine cultivars, 'Redsun' exhibited significantly ($p$ < 0.05) higher content of quercetin and rutin (135.7 and $169.9{\mu}g{\cdot}g^{-1}$ F.W., respectively), and S&P5296 had the highest content of myricetin($16.1{\mu}g{\cdot}g^{-1}$ F.W.). Breeding lines had more quercetin, rutin and myricetin contents than the cultivars 'Redprime' and 'Sunpower'. Hampyeong had the highest content for total phenol and total flavonoid, followed by Andong and Changyeong whereas Changyeong had the highest value for rutin, myricetin and quercetin followed by Hampyeong and Andong. Among red onion cultivars, 'Redsun' showed the highest value for anthocyanin, followed by S&P5295 and S&P5296, which the cultivar 'Redprime' had significantly (p<0.05) lower content(35.5 mg%).

• Horticultural Science & Technology
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• v.32 no.3
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• pp.390-399
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• 2014

The objective of this study was to establish a processing technology for preserved leaves based on the results from the examination of the optimal period and condition for dye-absorbing treatment for Eucalyptus cinerea F. Mull. ex Benth. (silver dollar eucalyptus) being used frequently as plant material for flower design. Cut foliages of E. cinerea with uniformly matured leaves were cut into 20 cm lengths and their lower stem parts were placed in dye solution in growth chambers with different temperatures (10, 20, 30, and $40^{\circ}C$), vapor pressure deficits (VPD; 0.23, 0.70, 1.17, and 1.61 kPa), and photoperiods (0, 6, 12, 24 hours) for 3, 6, 9, and 12 days, and then dried in a room of $20^{\circ}C$ for three days. Lower temperature during preserving dye treatment reduced the changes in leaf color compared with fresh leaves and decreased ${\Delta}E$ value. Especially, high temperature increased red degree (a) and decreased yellow degree (b) due to browning. Lower VPD reduced the change in leaf color compared with fresh leaves and decreased ${\Delta}E$ value. Shorter photoperiod reduced the change in leaf color compared with fresh leaves and decreased ${\Delta}E$ value. The ${\Delta}E$ value increased with increasing absorbing duration under three environmental conditions. The flexibility of stem and leaves after dipped into preserving dye solution and dried for 3 days increased with decreasing temperature, VPD and dipping duration. Therefore, the optimal environment condition for dye treatment was 0.23-0.70 kPa VPD at $10-20^{\circ}C$ in the darkness, and the optimal and economical duration was 3 days. These conditions reduced the speed of water loss by decreasing transpiration, so yellowing or browning by rapid water loss deteriorated the quality of preserved leaves out of these ranges.

• Horticultural Science & Technology
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• v.34 no.6
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• pp.959-965
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• 2016

In 1994, a new cultivar 'Joyskin' was created from a cross between the cultivars 'Whangkeumbae' and 'Waseaka' at the Pear Research Institute of the National Institute of Horticultural and Herbal Science, Rural Development Administration. In 2006, the 'Joyskin' was selected from among the 317 seedlings resulting from the cross for its skin and taste qualities. Regional adaptation tests were conducted in nine regions and in ten experimental plots from 2006 to 2011. The cultivar was named in 2011. 'Joyskin' showed a vigorous growth habit and semi-spread characteristics similar to 'Whangkeumbae'. The average full bloom date for 'Joyskin' was April 21st, which was also similar to 'Whangkeumbae'. The optimum fruit ripening time was September 6-8th, which was six or eight days earlier than 'Whangkeumbae'. The fruit was round in shape and the skin was a golden yellow color at maturity. The average fruit weight was 320 g and the flesh firmness was $2.5kg/8mm{\varphi}$. The firmness of the fruit skin determined by a blade-type plunger of texture analyzer was 22.9 N, which was significantly different from that of 'Whangkeumbae' 29.9N. Stone cell analysis of 'Joyskin' by phloroglucinol-HCl, showed that 'Joyskin' stone cells were small in size and few in numbers cpmpared to those of cultivars of was 'Manpungbae', 'Niitaka', and 'Whangkeumbae'. The patent application for 'Joyskin' was submitted in April, 2012 (Grant No. 2012-337). In 2016, 'Joyskin' (Grant No. 5895) was registered as a separate record, with uniformity and stability per Korean Seed Industry Law.

• Journal of The Korean Society of Grassland and Forage Science
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• v.15 no.3
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• pp.198-206
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• 1995

Baled silage making(BS) of selected forage crops was discussed during 1991-1993, to determine the best cutting time of the plants for BS production, BS yields and silage quality. Seven species of forage crops and pasture grasses(rye, barley, spring oat, Italian ryegrass, orchardgrass, alfalfa and grass-legume pasture mixtures) were harvested at different stage of growth from young plant to physiological maturity, and baled in a self constructed square baling chamber. Each bales, measured 90cm length, 60cm width and 50cm height, were wrapped with 0.05mrn thick polyethylene plastic film, and stored in stack silo. Each bales were weighed between 15-20 kg in dry matter basis. The effects of pre wilting and formic acid addition on the silage quality of young plant materials, which contained high water concentration, was also evaluated during the experiment. Rye plant including of barley and spring oat were evaluated as a good materials for baled silage making. Fodder rye produced high quality BS with a value of silage quality point 84(Flieg's point) when the plant harvested at stage of greatest dry matter accumulation by 12.64 tonha. The best quality BS of barley was obtained at stage of hard dough to yellow stage by 11.9 ton/ha dry matter yield and 81 point silage quality. Italian ryegrass and pasture grasses including of orchardgrass, alfalfa and grass-legume pasture mixtures procuced also high quality bale silage by harvesting at stage of late blooming. However pre witting operation and formic acid addition was required for BS production of grass materials because of high water contents. Water contents of Italian ryegrass and other pasture species ranged 18.9%(Italian) to 20.8%(alfalfa). Silage quality point of Italian BS harvested at late blooing was increased from 72 to 88 by 1/2-one day pre wilting and 0.3% formic acid treatment. Silage quality of young plant materials of rye and other forage crops, barley and spring oat were also improved markedly through the pre wilting treatment and formic acid addition.

• Journal of The Korean Society of Grassland and Forage Science
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• v.33 no.2
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• pp.94-99
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• 2013

Yanganok, a new single cross variety, was developed by the maize breeding team at the National Institute of Crop Science (NICS), RDA in 2012. This hybrid, which has a high yield of grain was produced by crossing two inbred lines, KS164 and KS163. KS164 is the seed parent and KS163 is the pollen parent of Yanganok. It is a yellow-orange intermediate maize hybrid (Zea mays L.). After a preliminary yield trial and advanced yield trial of Yanganok (Suwon180) in Suwon for 2 years, a regional yield trial was carried out for its growth characteristics, and yields were determined at 4 different locations from 2009 to 2012, with the exception of 2010. It was named 'Yanganok'. The silking date of Yanganok is 2 days earlier than that of the check hybrid, Kwangpyeongok. Plant height of Yanganok is similar to Kwangpyeongok, but its ear height ratio is higher than that of Kwangpyeongok. It has resistance to lodging. Stay-green features of Yanganok are similar to those of Kwangpyeongok. The ear ratio of Yanganok is similar to Kwangpyeongok. It has moderate resistance to southern leaf blight (Bipolaris maydis) and strong resistance to northern leaf blight (Exserohilum turcicum). It has moderate resistance to black streaked dwarf virus (BSDV), ear lot and corn borer. The dry matter yield of Yanganok, 17.45 ton/ha, was similar to that of Kwangpyeongok. The total digestible nutrients (TDN) yield of Yanganok, 11.96 ton/ha, was similar to that of Kwangpyeongok. The grain yield of Yanganok, 8.32 ton/ha was similar to that of Jangdaok. Seed production of Yanganok went well due to a good match during crossing between the seed parent, KS164, and the pollen parent, KS163, in Yeongwol, and F1 seed yield was 1.79 ton/ha. Yanganok would be a suitable cultivar to all plain areas in the Korean peninsula.