• Journal of Microbiology and Biotechnology
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• v.28 no.12
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• pp.2079-2094
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• 2018

Sunflower trypsin inhibitor (SFTI) is a 14-amino-acid bicyclic peptide that contains a single internal disulfide bond. We initially constructed chimeras of SFTI with N-terminal secretion signals from the Escherichia coli OmpA and Pseudomonas aeruginosa ToxA, but only detected small amounts of protease inhibition resulting from these constructs. A substantially higher degree of protease inhibition was detected from a C-terminal SFTI fusion with E. coli YebF, which radiated more than a centimeter from an individual colony of E. coli using a culture-based inhibitor assay. Inhibitory activity was further improved in YebF-SFTI fusions by the addition of a trypsin cleavage signal immediately upstream of SFTI, and resulted in production of a 14-amino-acid, disulfide-bonded SFTI free in the culture supernatant. To assess the potential of the secreted SFTI to protect the ability of a cytotoxic protein to kill tumor cells, we utilized a tumor-selective form of the Pseudomonas ToxA (OTG-PE38K) alone and expressed as a polycistronic construct with YebF-SFTI in the tumor-targeted Salmonella VNP20009. When we assessed the ability of toxin-containing culture supernatants to kill MDA-MB-468 breast cancer cells, the untreated OTG-PE38K was able to eliminate all detectable tumor cells, while pretreatment with trypsin resulted in the complete loss of anticancer cytotoxicity. However, when OTG-PE38K was co-expressed with YebF-SFTI, cytotoxicity was completely retained in the presence of trypsin. These data demonstrate SFTI chimeras are secreted in a functional form and that co-expression of protease inhibitors with therapeutic proteins by tumor-targeted bacteria has the potential to enhance the activity of therapeutic proteins by suppressing their degradation within a proteolytic environment.

• Journal of Welding and Joining
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• v.28 no.2
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• pp.91-95
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• 2010

Stress distribution and deformation on the CT-type(Cross Tension type) spot welded lap joint subjected to out of plane tensile load were investigated by finite element method. Using the maximum principal stresses at the nugget edge obtained by FEM analysis, evaluated the fatigue strength of the CT-type spot welded lap joints having various dimensions and materials. and also, the influence of the geometrical parameters of CT-type spot welded lap joints on stress distribution and fatigue strength must be evaluated. thus, in this paper, ${\Delta}P-N_f$ curve were obtained by fatigue tests. Using these results, ${\Delta}P-N_f$ curve were systematically rearranged in the $\Delta\sigma-N_f$ relation with the hot spot stresses at the CT-type spot welded lab joints. It was found that the proposed $\Delta\sigma-N_f$ relation could provide a more reasonable fatigue design criterion for the CT-type spot welded lap joints.

• Journal of Welding and Joining
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• v.28 no.3
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• pp.86-91
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• 2010

Stainless steel sheets are widely used as the structural material for the railroad cars and the commercial vehicles. These kinds structures used stainless steel sheets are commonly fabricated by using the gas welding. For fatigue design of gas welded joints such as fillet and plug type joint, it is necessary to obtain design information on stress distribution at the weldment as well as fatigue strength of gas welded joints. And also, the influence of the geometrical parameters of gas welded joints on stress distribution and fatigue strength must be evaluated. the ${\Delta}P-N_f$ curves were obtained by fatigue tests. Using these results, ${\Delta}P-N_f$ curves were rearranged in the ${\Delta}{\sigma}-N_f$ relation with the maximum stress at the edge of fillet welded joint.

• Transactions of the Korean Society of Automotive Engineers
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• v.18 no.4
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• pp.127-131
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• 2010

Stainless steel sheets are widely used as the structural material for the railroad cars and the commercial vehicles. These kinds of structures used stainless steel sheets are commonly fabricated by using the gas welding. For fatigue design of gas welded joints such as fillet and plug type joint, it is necessary to obtain design information on stress distribution at the weldment as well as fatigue strength of gas welded joints. And also, the influence of the geometrical parameters of gas welded joints on stress distribution and fatigue strength must be evaluated. Thus, in this paper, ${\Delta}P-N_f$ curves were obtained by fatigue tests. Using these results, ${\Delta}P-N_f$ curves were rearranged in the. ${\Delta}{\sigma}-N_f$ relation with the hot spot stresses at the gas welded joints.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.34 no.5
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• pp.561-565
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• 2010

Stainless steel sheets are widely used as structural materials for the manufacture of railroad cars and commercial vehicles. These kinds structures used stainless steel sheets are commonly fabricated by using the gas welding. For the fatigue design of gas welded joints such as fillet joints and plug joints, it is necessary to obtain information on the stress distribution at the weldment and the fatigue strength of the gas welded joints. Moreover the influence of the geometrical parameters corresponding to the gas welded joints on the stress distribution and fatigue strength must be evaluated. ${\Delta}P-N_f$ curves were obtained from the data recorded in fatigue tests. Using these results, the ${\Delta}P-N_f$ curves were rearranged according the relation between $\Delta\sigma-N_f$ and the maximum stress at the edge of the fillet welded joint.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.16 no.7
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• pp.4398-4404
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• 2015

Stainless steel sheets are widely used as the structural material for dynamic machine structures, These kinds structures used stainless steel sheets are commonly fabricated by using the gas welding, For fatigue design of gas welded joints such as various type joint. It is necessary to obtain design information on stress distribution at the weldment as well as fatigue strength of gas welded joints. Thus in this paper, ${\Delta}P-N_f$ curves were obtained by fatigue tests. and, ${\Delta}P-N_f$ curves were rearranged in the ${\Delta}{\sigma}-N_f$ relation with the hot spot stresses at the gas welded joints. Using these results, the accelerated life test(ALT) is conducted. From the experiment results, an life prediction model is derived and factors are estimated. So it is intended to obtain the useful information for the fatigue lifetime of welded joints and data analysis by statistic reliability method, to save time and cost, and to develop optimum accelerated life prediction plans.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.34 no.4
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• pp.467-474
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• 2010

Stainless steel sheets are widely used as the structural material for railroad cars and commercial vehicles. Structures made of stainless steel sheets are commonly fabricated by gas welding, For the fatigue design of gas welded joints such as fillet joints, it is necessary to obtain design information of the stress distribution at the weldment as well as the fatigue strength of the gas-welded joints. Further, the influence of the geometrical parameters of gas-welded joints on stress distribution and fatigue strength must be evaluated. in this study, ${\Delta}P-N_f$ curves were obtained by fatigue tests. and, the ${\Delta}P-N_f$ curves were rearranged on the basis of the ${\Delta}{\sigma}-N_f$ relation for the hot-spot stresses at the gas-welded joints. These results, were used for conducting an accelerated life test(ALT) From the experiment results, an acceleration model was derived and factors were estimated. The objective is to obtain the information required for the analysis of the fatigue lifetime of fillet welded joints and for data analysis by the statistic reliability method to save time and cost and to develop optimum accelerated life prediction plans.

• Proceedings of the KSME Conference
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• 2008.11a
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• pp.1355-1360
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• 2008

Stainless steel sheets are widely used as the structure material for the railroad cars and the commercial vehicles. These kinds structures used stainless steel sheets are commonly fabricated by using the gas welding. Gas welding is very important and useful technology in fabrication of an railroad car and vehicles structure. However fatigue strength of the gas welded joints is considerably lower than parent metal due to stress concentration at the weldment, fatigue strength evaluation of gas welded joints are very important to evaluate the reliability and durability of railroad cars and to establish a criterion of long life fatigue design. In this paper, ${\Delta}P-N_f$ curve were obtained by fatigue tests. Using these results, the accelerated life test (ALT) is conducted. From the experimental results, an acceleration model is derived and acceleration factors are estimated. So it is intended to obtain the useful information for the fatigue lifetime of plug and ring gas welded joints and data analysis by statistic reliability method, to save time and cost, and to develop optimum accelerated life prediction plans.

• Journal of Welding and Joining
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• v.28 no.1
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• pp.77-85
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• 2010

Stainless steel sheets are widely used as the structure material for the railroad cars and the commercial vehicles. These kinds structures used stainless steel sheets are commonly fabricated by using the gas welding. Gas welding is very important and useful technology in fabrication of a railroad car and vehicles structure.However fatigue strength of the gas welded joints is considerably lower than parent metal due to stress concentration at the weld, fatigue strength evaluation of gas welded joints are very important to evaluate the reliability and durability of railroad cars and to establish a criterion of long life fatigue design. In this paper, $({\Delta}{\sigma}_a)_R-N_f$ curve were obtained by fatigue tests. Using these results, the accelerated life test(ALT) was conducted. From the experimental results, an acceleration model was derived and acceleration factors are estimated. So it is intended to obtain the useful information for the fatigue lifetime of plug and ring gas welded joints and data analysis by statistic reliability method, to save time and cost, and to develop optimum accelerated life prediction plans.

• Transactions of the Korean Society of Automotive Engineers
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• v.19 no.3
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• pp.1-8
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• 2011

Stainless steel sheets are widely used as the structure material for the railroad cars and the commercial vehicles. These kinds structures used stainless steel sheets are commonly fabricated by using the gas welding. Gas welding is very important and useful technology in fabrication of an railroad car and vehicles structure. However fatigue strength of the gas welded joints is considerably lower than parent metal due to stress concentration at the weldment, fatigue strength evaluation of gas welded joints are very important to evaluate the reliability and durability of railroad cars and to establish a criterion of long life fatigue design. In this paper, ${\Delta}-N_f$ curve were obtained by fatigue tests. Using these results, the accelerated life test (ALT) is conducted. From the experimental results, an acceleration model is derived and acceleration factors are estimated. So it is intended to obtain the useful information for the fatigue lifetime of plug and ring gas welded joints and data analysis by statistical reliability method, to save time and cost, and to develop optimum accelerated life prediction plans.