• Journal of the Korea Academia-Industrial cooperation Society
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• v.16 no.1
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• pp.438-444
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• 2015

The aim of this research was to apply a HACCP system (Hazard Analysis Critical Control Point) to fermented milk. The main ingredients of fermented milk, work facilities and workers were obtained from a company named YD, which is located in Seobuk-gu, Cheonan-si between November 5 2013 to April 13, 2014. A manufacturing process chart was prepared by referring to the manufacturing process of fermented milk manufacturers in common. The manufacturing process chart was made with raw materials; Raw milk, High Fructose Corn Syrup, Oligosaccharides, Lactic Acid bacteria and Subsidiary ingredients, Warehousing of packaging materials, Storage, Input, Preheating, Mixing, Homogeneity, Sterilization, Precooling, Culture, Filtration, In packaging, Out packaging, Storage, and Consignment, as listed Table 1. The results of the microbiological hazard analysis on the raw materials was safe after sterilization($90^{\circ}C{\pm}5^{\circ}C$, $35{\pm}3min.$) On the other hand,a microorganism test of an environment and workers suggested that the microbiological hazard should be reduced through systematic cleaning and disinfection accompanied by improved personal hygiene based on hygienic education on workers and the management of microorganisms in air.

• Journal of Biomedical Engineering Research
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• v.38 no.4
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• pp.175-182
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• 2017

Microcurrent electrical stimulation(MES) has been used to accelerate recovery of atrophied skeletal muscle. However, convincing stimulation parameters for suppressing muscle atrophy due to injured sciatic nerve remains unclear. The objective of this study was to investigate the effective intensity of MES on restraining muscle atrophy with rat model underwent sciatic nerve injury(SNI). Twenty-5-week-old Sprague Dawley male rats were equally assigned to five groups : Control group(Control, CON, n = 4), Denervation group(Denervation, D, n = 4), Denervation with MES of $22{\mu}A$ group(Denervation + $22{\mu}A$, D+22, n = 4), Denervation with MES of $100{\mu}A$ group (Denervation + $100{\mu}A$, D+100 n = 4), Denervation with MES of $400{\mu}A$ group(Denervation + $400{\mu}A$, D+400, n = 4). To induce muscle atrophy, all rats in the D, D+22, D+100, and D+400 groups, were subjected to sciatic nerve injury on their right hindlimb and allowed to have 1 week of resting period. Following this period, rats underwent daily MES(60 min/ a day, 5times/1week) for 4 weeks. After that, we investigate morphological changes in muscle volume by using in vivo micro-computed tomography at week 0, 1, 3 and 5. After 5 weeks, the muscle volume had the highest value in D+400 group, and also noticeably increased in D+100 group compared to it in D group. The results of this study imply that MES with current intensities between $100-400{\mu}A$ can suppress muscle atrophy effectively.

• Korean Journal of Clinical Laboratory Science
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• v.45 no.2
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• pp.48-53
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• 2013

Recently, the isolation rate of nontuberculous mycobacteria (NTM) in clinical laboratories and the incidence of NTM infections are on the increase in Korea, but there have been only a few studies that reveal the general aspect of NTM isolation or species distribution. Therefore, this study was performed to examine the species identification by PCR-restriction fragment length polymorphism analysis (PRA, PCR-RFLP), and the clinical significance of mycobacterial cultures. PRA was used during the novel region of the rpoB gene and was developed for rapid and precise identification of mycobacteria to the species level. From January 2012 to April 2012, we examined pre-identified nontuberculous mycobacteria (60 species in 3 hospital of Busan-Kyeongnam area). We confirmed 4 (6.6%) Mycobacterium tuberculosis (MTB) and 56 (93.4%) NTM from 60 pre-identified NTM species by multiplex PCR (MolecuTech $MTB-ID^R$ V3, YD Diagnostics, Korea) and PRA (Myco-ID, YD Diagnostics, Korea). The distribution of 56 NTM species were M. intracellulare type I 15 (26.7%), M. avium 14 (25%), M. abscessus 11 (19.5%), M. kansasii type I 3 (5.4%), M. pulveris 2 (3.6%), M. intracellulare type, M. chelonae, M. kansasii type V, M. gallinarum, M. wolinskyi. Respectively, 1 (1.8%) and 6 (10.7%) species were not identified.

• Korean Journal of Medicinal Crop Science
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• v.21 no.6
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• pp.486-492
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• 2013

This study has been conducted to establish the optimal extraction process and HPLC analysis method for the determination of marker compounds as a part of the materials standardization for the development of health functional food materials from Astragali radix. Five extraction conditions including the shaking extraction at room temperature and the reflux extraction at $85^{\circ}C$ with 30%, 50% and 95% ethanol were evaluated. Reflux extraction with 50% ethanol showed the highest extraction yield as $27.27{\pm}2.27%$, while the extraction under reflux with 95% ethanol showed significantly the lowest yield of $10.55{\pm}0.24%$. The quantitative determination methods of calycosin-7-O-${\beta}$-D-glucoside and calycosin as marker compounds of Astragali radix extracts were optimized by HPLC analysis using a Thermo Hypersil column ($4.6{\times}250mm$, $5{\mu}m$) with the gradient elution of water and acetonitrile as the mobile phase at the flow rate of $0.8mLmin^{-1}$ and a detection wavelength of 230nm. The HPLC/UV method was applied successfully to the quantification of two marker compounds in Astragali radix extracts after validation of the method with the linearity, accuracy and precision. The contents of calycosin-7-O-${\beta}$-D-glucoside and calycosin in 50% ethanol extracts by reflux extraction were significantly higher as $1,700.3{\pm}30.4$ and $443.6{\pm}8.4{\mu}g-1$, respectively, comparing with those in other extracts. The results indicate that the reflux extraction with 50% ethanol at $85^{\circ}C$ is optimal for the extraction of Astragali radix, and the established HPLC method are very useful for the evaluation of marker compounds in Astragali radix extracts to develop the health functional material from Astragali radix.

• Journal of Physiology & Pathology in Korean Medicine
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• v.29 no.3
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• pp.230-239
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• 2015

This study was performed to find a relationship between each pattern identification and vascular status using the second derivative of photoplethysmogram waveform(SDPTG) indices. We analyzed 200 subjects who participated in stroke preventive examination. We classified the subjects into four groups of pattern identifications; Fire-Heat pattern(火熱證; FH), Yin Deficiency pattern(陰虛證; YD), Qi Deficiency pattern(氣虛證; QD) and Dampness-Phlegm pattern(濕痰證; DP) that based on Korean Standard Pattern Identifications for Stroke-Ⅲ. We studied a relationship between each pattern identification and the SDPTG. The total number of the subject group was 200, whereas the groups were divided into four groups; Fire-Heat pattern group(n=49), Yin Deficiency pattern(n=57), Qi Deficiency pattern(n=45), and Dampness-Phlegm pattern(n=49). b/a ratio was related with age and systolic blood pressure, c/a ratio was associated with age, systolic blood pressure, fasting blood sugar and Total cholesterol, d/a ratio was affected with age, diastolic blood pressure, and hypertension, e/a ratio was related with age and sex and SDPTG AI was associated with age. c/a ratio and d/a ratio were significantly higher in the Fire-Heat group than in the Qi Deficiency group. SDPTG AI was significantly higher in the Qi Deficiency group than in the Fire-Heat group. The Qi Deficiency group was significantly older than the Fire-Heat group and the number of hypertension patients was significantly more in the Fire-Heat group than in the Qi Deficiency group. Through this study, we found out some significant relationships between each pattern identification group and the SDPTG indices.

• Journal of the Korean Society of Clothing and Textiles
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• v.26 no.10
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• pp.1443-1454
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• 2002

The purposes of this study were to classify body type of adult males into several kind of shape and to provide the characteristics of size of each group which has same shape. As the sample, subjects were 1290 males of 20 to 54 year-old. The procedure and results were follows; 1. As the result of the previous reserch, the front line of body was classified in X, H, Y and A types. 2. The principal component analysis was used to obtain the shape factor of the side line of the trunk. 9 factors in the side were extracted. As the result of the cluster analysis of factor scores, the side line of body was classified in 5 types. It was named X, A, Y and H type in the front and S, D1, d, I and D2 type in the side. 3. In order to consider the shape of body as a whole, the body shape of the front and side were compounded. The whole body shapes of adult male were very various, and 6 body shapes, XS, YS, Yd, YI, AD2 and HD1 were selected as the basic types. In each type of body, several groups were classified by size factor, height and chest girth and master size was selected considering appearance frequency.

• Proceedings of the Korean Society of Dyers and Finishers Conference
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• 2011.03a
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• pp.102-102
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• 2011

레이온 섬유는 dry한 촉감, 고광택, 드레이프성 등 의류 분야에서 요구하는 많은 장점들을 가지고 있으나 타 섬유 소재에 비해 소비량이 적은 것은 합섬이나 면/울 제품에 비해 상대적으로 제품 가격이 높고, 형태안정성이 취약하여 정장 및 캐주얼의 겉감 용도로 쓰기에는 성능 보완이 필요하다. 또한 염색가공 공정에서 구김발생이 많으며, 열고정이 쉽게 이루어지지 않아 습윤강도와 탄성 회복률이 낮아 변형이 쉽게 발생된다. 이에 본 연구에서는 합섬의 장점을 그대로 유지하면서 레이온 섬유가 갖는 고비중과 우수한 드레이프성과 유연한 질감으로 소프트 터치를 발현하는 특수한 레이온 대체 소재를 실현하고자 하였다. 직물에서 드레이프성과 은은한 광택을 확보하기 위해서는 폴리머단계에서 비중과 광택을 발현할 수 있는 무기입자 중 비중이 높고, 중합 후 폴리머 내에서 광택을 유지하는 입자의 선택이 필요한데 본 실험에서는 $BaSO_4$를 이용하여 PET dope액과 중합한 후 용융 방사하여 고비중 폴리에스테르사(100/48)를 제조하였다. 고비중 폴리에스테르사를 이용하여 폭 58inch, 밀도 92T, 중량 324.8g/yd 직물로 제직하여 그에 따른 태를 측정하였다. 태측정기(KES-FB, KATO TECH CO)를 이용하여 인장 & 전단강도, 굽힘강도, 압축강도, 표면측정 시험 결과 $BaSO_4$ 2% 첨가한 원단의 드레이프성이 우수한 것을 확인할 수 있었다.

• Journal of dental hygiene science
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• v.17 no.5
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• pp.433-438
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• 2017

Relative to its incidence, oral cancer has serious negative social effects. The exact causes of oral cancer have not been clarified, but many studies have implicated smoking and drinking. However, the fundamental mechanism of oral cancer causation has yet to be elucidated. Lysophosphatidic acid (LPA) augments epithelial mesenchymal transition (EMT) and development of various cancer cells. However, a detailed mechanistic explanation for LPA-induced EMT and the effects of EMT-promoting conditions on oral squamous cell carcinoma development remain elusive. In the present study, a quantitative reverse transcription polymerase chain reaction was used to analyze TWIST1, Slug, E-cadherin, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) transcript expression. Immunoblotting was used to analyze TWIST1, Slug, E-cadherin, and GAPDH protein expression. siRNAs were used to silence TWIST1 and Slug transcript expression. A matrigel-coated in vitro invasion insert was used to analyze oral cancer cell invasion. The results of the present study show that the expression levels of TWIST1 and Slug, which are EMT factors, were increased by LPA treatment in YD-10B oral squamous cell carcinoma. Conversely, E-cadherin expression was significantly reduced. In addition, transfection of the cells with TWIST1 and Slug siRNA strongly inhibited LPA-induced oral cancer cell invasion. The present study shows that TWIST1 and Slug mediate LPA-induced oral cancer cell EMT and invasiveness. The present study confirmed the mechanism by which LPA promotes oral cancer cell development, with TWIST1 and Slug providing novel biomarkers and promising therapeutic targets for oral cancer cell development.

• Journal of Pharmacopuncture
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• v.23 no.2
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• pp.79-87
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• 2020

Objectives: Ginsenosides found in ginseng, and the hydrolysates derived from their conversion, exhibit diverse pharmacological characteristics [1]. These have been shown to include anti-cancer, anti-angiogenic, and anti-metastatic effects, as well as being able to provide hepatic and neuroprotective effects, immunomodulation, vasodilation, promotion of insulin secretion, and antioxidant activity. Therefore, the purpose of this study was to examine how quickly the ginsenosides decompose and what kinds of degradation products are created under physicochemical processing conditions that don't involve toxic chemicals or other treatments that may be harmful. Methods: The formation of ginsenoside-Rg2 and ginsenoside-Rg3 was examined. These demonstrated diverse pharmacological effects. Results: We also investigated physicochemical factors affecting their conversion. The heating temperatures and times yielding the highest concentration of ginsenosides (-Rb1, -Rb2, -Rc, -Rd, -Rf, -Rg1, and -Re) were examined. Additionally, the heating temperatures and rates of conversion of these ginsenosides into new 'ginseng saponins', were examined. Conclusion: In conclusion, obtained provide us with effective technology to control the concentration of both ginsenosides and the downstream converted saponins (ginsenoside-Rg2, Rg3, Rg5, and Rk1 etc.), as well as identifying the processing conditions which enable an enrichment in concentration of these compounds.

• Korean Journal of Pharmacognosy
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• v.43 no.1
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• pp.22-26
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• 2012

The aim of this study was to evaluate the anti-inlfammatory effect of the extract of Zanthoxylum schinifolium on ulcerative colitis induced by 3% dextran sulfate sodium in the rat. The experiment animals were divided into six groups: control (normal), DDS-induced colitis, 1 mg/kg, 15 mg/kg and 150 mg/kg of the extract of Z. schinifolium and 150 mg/kg of 5-aminosalicylic acid (5-ASA) as comparative drug. Anti-ulcerative colitis effect was evaluated pathologically by disease activity index (DAI), the change of weight and myeloperoxidase (MPO) in colon mucosa. Treatment with 15 mg/kg and 150 mg/kg of the extract of Z. schinifolium significantly improved the gain of body weight and DAI as clinical symptoms, and reduced MPO and $PGE_2$ level as biochemical index. Especially, 150 mg/kg of the extract of Z. schinifolium showed markedly more improvement than the same dose of 5-ASA in all kind of index such as MPO, $PGE_2$ and DAI. These results suggest that Z. schinifolium mediated anti-inflammatory action on colorectal sites may be a useful therapeutic approach to ulcerative colitis.