• 한국기후변화학회지
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• 제9권3호
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• pp.245-251
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• 2018

Creating Shared Value (CSV) is a new business paradigm in which enterprises benefit from social works. The goal of this study is to assess greenhouse gas emission reduction through CSV-related activities of Yuhan-Kimberly (YK) Company. YK Company has planted over 50 million trees between 1984 and 2016 as a part of CSV activities. Through planting of trees, annual $CO_2 $ emission reduction ranged from 196.2 thousand to $336.3\;thousand\;tCO_2-eq$ depending on forest type in 2016, representing 44 million to 84 million KRW. Those results indicate that the company can contribute to reduction of greenhouse gas emissions as well as obtain economic profits through CSV-related activities. Furthermore, this study provides motives for other companies interested in similar CSV projects.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제42권
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• pp.6.1-6.6
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• 2020

Background: Various types of miniplates have been developed and used for the reduction of facial bone fractures. We introduced Yang's Keyhole (YK) plate, and reported on its short-term stability. The purpose of this study was to evaluate the long-term stability of the YK plate, as a follow-up study, by examining the patients who had used the YK plate among the patients with the reduction of mandible fractures and who visited for plate removal. Methods: We reviewed the medical records of 16 patients who underwent mandibular fracture fixation using a YK plate (group I) and 17 patients who underwent mandibular fracture fixation using a conventional plate (group II). Assessment was then made on malunion, occlusal stability, discomfort during the application, and clinical symptoms. Results: From January 2015 to December 2017, a total of 36 patients underwent mandibular fracture surgery using a YK plate. A total of 16 patients received plate removal. Among them, 15 were male and 1 female. The average age was 26 years. The applied surgical sites were the 12 on mandibular angle, 4 on mandibular symphysis, and 2 on subcondyle. The application period of YK plate was an average of 335 days. During the same period, 45 people underwent surgery on the conventional plate. A total of 17 patients received plate removal. Among them, 15 were male and 2 females. The average age was 36 years. The applied surgical sites were the 8 on mandibular angle, 4 on mandibular symphysis, and 2 on subcondyle. The application period of the conventional plate was an average of 349 days. No malocclusion occurred at the time of removal, and occlusion was stable. No patient complained of joint disease or discomfort. Conclusion: The YK plate system, in which the screw was first inserted and the plate was applied, for clinical convenience did not cause any particular problem and no significant difference from the conventional plate.

• Animal cells and systems
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• 제9권2호
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• pp.87-94
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• 2005

Spindle position checkpoint monitors the orientation of mitotic spindle for proper segregation of replicated chromosomes into mother cell and the daughter, and prohibits mitotic exit when mitotic spindle is misaligned. BUB2 forms one of the key upstream element of spindle position checkpoint in budding yeast, but its functional homologues have not been identified in higher eukaryotes. Here, we analyzed the functions of two putative BUB2 homologues of C. elegans in the spindle orientation checkpoint. From the C. elegans genome database, we found that two open reading frames (ORFs), F35H12_2 and C33F10_2, showed high sequence homology with BUB2. We obtained the expressed sequence tag (EST) clones for F35H12_2 (yk221d4) and C33F10_2 (yk14e10) and verified the full cDNA for each ORF by sequencing and 5' RACE with SL1 primer. The functional complementation assays of yk221d4 and yk14e10 in ${\Delta}bub2$ of S. cerevisiae revealed that these putative BUB2 homologues of C. elegans could not replace the function of BUB2 in spindle position checkpoint and mitotic exit. Our attempt to document the component of spindle position checkpoint in metazoans using sequence homology was not successful. This suggests that structural information about its components might be required to identify functional homologues of the spindle position checkpoint in higher eukaryotes.

• Biotechnology and Bioprocess Engineering:BBE
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• 제10권3호
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• pp.180-185
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• 2005

Cytosine deaminase (cytosine aminohydrolase, EC 3.5.4.1) stoichiometrically catalyzes the hydrolytic deamination of cytosine and 5-fluorocytosine to uracil and 5-fluorouracil, respectively. Amino acid residues located in or near the active sites of the intracellular cytosine deaminase from chromobacterium violaceum YK 391 were identified by chemical modification studies. The enzymic activity was completely inhibited by chemical modifiers, such as 1mM NBS, chloramine-T, $\rho-CMB,\;\rho-HMB$ and iodine, and was strongly inhibited by 1mM PMSF and pyridoxal 5'-phosphate. This chemical deactivation of the enzymic activity was reversed by a high concentration of cytosine. Furthermore, the deactivation of the enzymic activity by $\rho-CMB$ was also reversed by 1mM cysteine-HCI, DTT and 2-mercaptoethanol. These results suggested that cysteine, tryptophan and methionine residues might be located in or near the active sites of the enzyme, while serine and lysine were indirectly involved in the enzymic activity. The intracellular cytosine deaminase from C violaceum YK 391 was assumed to be a thiol enzyme.

• Journal of Microbiology and Biotechnology
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• 제14권6호
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• pp.1182-1189
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• 2004

Cytosine deaminase (cytosine aminohydrolase, EC 3.5.4.1) stoichiometrically catalyzes the hydrolytic deamination of cytosine and 5-fluorocytosine to uracil and 5-fluorouracil, respectively. The intracellular cytosine deaminase from Chromobacterium violaceum YK 391 was purified to apparent homogeneity with 272.9-fold purification with an overall yield of $13.8\%$. The enzyme consisted of dimeric polypeptides of 63 kDa, and the total molecular mass was calculated to be approximately 126 kDa. Besides cytosine, the enzyme deaminated 5-fluorocytosine, cytidine, 6-azacytosine, and 5-methylcytosine, but not 5-azacytosine. Optimum pH and temperature for the enzyme reaction were 7.5 and $30^{\circ}C$, respectively. The enzyme was stable at pH 6.0 to 8.0, and at 30T for a week. About $70\%$ of the enzyme activity was retained at $60^{\circ}C$ for 5 min. The apparent $K_{m}$ values for cytosine, 5-fluorocytosine, and 5-methylcytosine were calculated to be 0.38 mM, 0.87 mM, and 2.32 mM, respectively. The enzyme activity was strongly inhibited by 1 mM $Hg^{2+},\;Zn^{2+},\;Cu^{2+},\;Pb^{2+},\;and\;Fe^{3+}$, and by o-phenanthroline, $\alpha,\;{\alpha}'$-dipyridyl, p-choromercuribenzoate, N-bromosuccinimide, and cWoramine­T. In addition, the enzyme activity was strongly inhibited by I mM 2-thiouracil, and weakly inhibited by 2-thiocytosine, or 5-azacytosine. Finally, intracellular and extracellular cytosine deaminases from Chromobacterium violaceum YK 391 were found to have a different optimum temperature, apparent $K_{m}$ value, and molecular mass.

• 펄프종이기술
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• 제31권2호
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• pp.8-14
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• 1999

A screening has been performed to find hyper-ligninolytic fungi, which degtrade beech and pine lignin extensively in order to broaden the understanding of the ligninolytic enzymes elaborated by various white-rot fungi. One hundred and twenty two ligninolytic strains were selected from decayed woods with a selective medium for screening ligninolytic wood-rotting fungi. Two of them, Phanerochaete sordida YK-624 and YK-472, showed much higher ligninolytic activity and selectivity in beech-wood degradation than typical lignin-degrading fungi, phanerochaete chrysosporium and Coriolus versicolor. They also degraded birch dioxane lignin and residual lignin in unbleached kraft pulp(UKP) much more extensively than P. chrysosporium and C. versicolor. During fungal treatment of beech wood-powder, the fungus strain P. sordida YK-624 showed higher activity of extracellular manganese peroxidase (MnP) in the medium than P. chrysosporium. It also showed MnP activity, which would not be lignin peroxidast during treatment of oxygen-bleached kraft pulp(OKP) and under enzyme-inducing conditin.

• Journal of Microbiology and Biotechnology
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• 제19권6호
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• pp.588-595
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• 2009

A bacterial strain, YK-2, was isolated as a producer of trans glutaminase from a forest soil sample of Daegu, Korea. The isolate showed a G+C content of 72.7 mol%, contained meso-$A_2pm$ as the cell-wall amino acid, and possessed menaquinone MK-9 ($H_6$) and menaquinone MK-9 ($H_8$) at a ratio of 6:4. The chemotaxonomic analysis, as well as phylogenetic analysis based on the 16S rDNA sequence, identified the isolate as a member of Streptomyces platensis. For transglutaminase production, the optimum medium composition was determined to be 2% glucose, 1% polypeptone, 1% soy tone, and 0.1% $MnCl_2$. The transglutaminase was stable within the pH range of 5.0-9.0 and $30-45^{\circ}C$, and the optimum pH and temperature were pH 8.0 and $45^{\circ}C$, respectively, without any requirement for $Ca^{2+}$.

• 한국정보처리학회논문지
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• 제7권10호
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• pp.3138-3147
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• 2000

전자상거래는 인터넷이라는 가상 공간을 통해 이루어지므로, 시간과 장소에 구애받지 않는다는 강점이 있는 반면, 누구나 쉽게 접근할 수 있는 개방형 네트워크의 특성에 따라 보안상의 문제점이 대두될 수 있다는 약점을 동시에 갖고 있다. 따라서, 안전하고 효율적인 전자상거래 구현을 위해서는 전자상거래 보안상의 문제를 해결하기 위한 장치가 필요한데, 이러한 장치 중에 하나가 견고한 암호 알고리즘의 구축이라 할 수 있겠다. 본 논문에서 제안한 YK2(Young Ku Kang) 암호 알고리즘은 이러한 전자상거래 보안을 충족시켜주기에 적합한 시스템으로서, 기본적으로 입·출력의 키와 암호화에 사용되는 키의 크기가 각각 128비트로 구성되고 32회전으로 전개됨으로서 기존의 64비트를 사용했던 블록 암호 알고리즘의 한계를 극복할 수 있다. 또한, 암호화에 민감한 영향을 미치는 요소 중에 하나인 키 스케줄링을 보다 복잡하게 설계함으로써 계산 복잡도의 증가와 확률 계산의 증가를 도모하였다.

• The Korean Journal of Ecology
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• 제9권1호
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• pp.41-50
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• 1986

The net primary production in the Korean peninsula was estimated by Miami model, Montreal model and Kira's model, based on 148 meteorological data. The modes in frequency distribution of the values calculated by Montreal and Miami model were found at 1,500g/m2/yr. class and at one step high class in 100g. interval, while by Kira's madel at 1,700g/m2/yr. class. The relationships between values by Miami model(X) and those by Motreal model (Ym) and Kira's model(Yk) can be expressed as follows: Ym=0.365X+944.7, Yk=0.462 X+1006.9 and Yk=1.282Ym-211.5. The total amount of the net primary production in 218,583.4km2, 98.9% of the whole area(220,951 km2) of the Korean Peninsula, was estimated as 290,691,407 tons/yr. by Miami model, 310,751,566 tons/yr by Montreal model and 352,071,901 tons/yr by Kira's model. Therefore, it is reasonable that the organic substance over 300 million-tons is added yearly in the Korean Peninsula, because only 1.1% of the whole area no calculated. In additiion, the net primary production amount of Han-river basin was estimated as ca. 38 million-tons, whether calculated with the meteorological data in level of the Korean Peninsula or with more detail data.

• Journal of applied mathematics & informatics
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• 제23권1_2호
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• pp.311-319
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• 2007

This article studies a modified BFGS algorithm for solving smooth unconstrained strongly convex minimization problem. The modified BFGS method is based on the new quasi-Newton equation $B_k+1{^s}_k=yk\;where\;y_k^*=yk+A_ks_k\;and\;A_k$ is a matrix. Wei, Li and Qi [WLQ] have proven that the average performance of two of those algorithms is better than that of the classical one. In this paper, we prove the global convergence of these algorithms associated to a general line search rule.