• Microbiology and Biotechnology Letters
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• v.24 no.5
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• pp.604-612
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• 1996

To improve the fermentation characteristics of the haploid starch-fermenting recombinant yeast strain K114/YIpMS$\Delta$R(LEU2/URA3) secreting both $\alpha$-amylase and glucoamylase was rare-mated with polyploid industrial yeast Saccharomyces sp. K35. The K35 strain had good fermentation-characteristics such as ethanol-tolerance, high temperature and sugar-tolerance, and high fermentation rate. Among the resulting 66 hybrids, the best strain RH51 was selected. The RH51 exhibited amylolytic activity of K114/YIpMS$\Delta$R(LEU2/URA3) as well as ethanol and sugar tolerance of K35. The optimum temperature of hybrid RH51 for starch fermentation was 34$\circ$C which was same as that of K35 but different from that (30$\circ$C) of K114/YIpMS$\Delta$R(LEU2/URA3). The optimum pH was 5.0. The optimum size of inoculum was 2% as the pellet (w/v) of yeast cells. The hybrid strain RH51 produced 7.0% ethanol (w/v) from 20% (w/v) soluble starch while K35 formed almost no ethanol, 0.3% (w/v). RH51 strain produced 7.5% (w/v) ethanol after 8 days in a 2.5 l fermenter containing 800 ml of 20% (w/v) soluble starch. The residual starch content in the fermentation medium was 1.68% (w/v), and therefore almost all the starch was fermented completely.

• Asian-Australasian Journal of Animal Sciences
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• v.33 no.3
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• pp.398-407
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• 2020

Objective: The Yip1 domain family (YIPF) proteins were proposed to function in endoplasmic reticulum (ER) to Golgi transport and maintenance of the morphology of the Golgi, which were homologues of yeast Yip1p and Yif1p. YIPF3, the member 3 of YIPF family was a homolog of Yif1p. The aim of present study was to investigate the expression and regulation mechanism of porcine YIPF3. Methods: Quantitative realtime polymerase chain reaction (qPCR) was used to analyze porcine YIPF3 mRNA expression pattern in different tissues and pig kidney epithelial (PK15) cells stimulated by polyinosine-polycytidylic acid (poly [I:C]). Site-directed mutations combined with dual luciferase reporter assays and electrophoretic mobility shift assay (EMSA) were employed to reveal transcription regulation mechanism of porcine YIPF3. Results: Results showed that the mRNA of porcine YIPF3 (pYIPF3) was widely expressed with the highest levels in lymph and lung followed by spleen and liver, while weak in heart and skeletal muscle. Subcellular localization results indicated that it expressed in Golgi apparatus and plasma membranes. Upon stimulation with poly (I:C), the level of this gene was dramatically up-regulated in a time- and concentration-dependent manner. pYIPF3 core promoter region harbored three cis-acting elements which were bound by ETS proto-oncogene 2 (ETS2), zinc finger and BTB domain containing 4 (ZBTB4), and zinc finger and BTB domain containing 14 (ZBTB14), respectively. In which, ETS2 and ZBTB4 both promoted pYIPF3 transcription activity while ZBTB14 inhibited it, and these three transcription factors all played important regulation roles in tumorigenesis and apoptosis. Conclusion: The pYIPF3 mRNA expression was regulated by ETS2, ZBTB4, and ZBTB14, and its higher expression in immune organs might contribute to enhancing ER to Golgi transport of proteins, thus adapting to the immune response.

• Microbiology and Biotechnology Letters
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• v.39 no.2
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• pp.111-118
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• 2011

For the strain improvement of the industrial polyploid yeast strain through hybridization and protoplast fusion, a dominant selection marker other than a recessive marker such as the auxotrophic marker was required for the selection of the resulting hybrids. In the present investigation, the aureobasidin A resistant gene was tested in relation to whether it can be used as the dominant selectable marker for the isolation of hybrids of the yeast Saccharomyces. The plasmid pAUR112, carrying the gene responsible for resistance to aureobasidin A, was introduced into the haploid yeast strain K114/YIp. From the rare-mating between polyploid C6 and haploid K114/YIp carrying pAUR112, many hybrids were obtained from the agar medium containing 0.5 ${\mu}g$/ml of aureobasidin A. The hybrids exhibited characteristics derived from both of the parental strains; and the cell sizes of the hybrids were larger than those of the parental strains. These results showed that the aureobasidin A resistant gene could be successfully used as the dominant selectable marker for the isolation of yeast hybrids resulting from rare-mating.

• Safety and Health at Work
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• v.6 no.2
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• pp.156-156
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• 2015

• 월간산업보건
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• s.281
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• pp.69-71
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• 2011

• The Journal of Korean Medical History
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• v.15 no.2
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• pp.117-134
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• 2002

"Gup Yu Bang" is the first Korean book that specialized in pediatrics. It was written by Jo Jeong-jun on the 25th year of King Young Jo in the Choson dynasty. He quoted a lot of information from "Yi-Hak-Yip-Mun" by Yi-cheon in the Myung dynasty and "Xiao Er Yao Zheag Zhi Jue" by Qian Yi in the Song dynasty, but he added his own opinions and clinical experiences to the book. In addition to that, the book explains not only symptoms and prescriptions but also the etiology and pathology of children's diseases. In particular, he showed a great deal of creativity in his book called "Dong Bang Six Fu's Qi Principle" After studying "Gup Yu Bang", I have drawn the following conclusions. 1. Prevention is more important than treatment in pediatrics. 2. When we make a diagnosis, facial inspection has priority. 3. In terms of treatmemt, Wu zang is a general rule. Therefore, we should focus on Wu zang and Pi Wei. 4. The following terms definition symptom cause prescription clinical example prevention are to be explained with reference to the nature or symptoms of the disease. 5. We can see that smallpox was more widspread than the measles in the period of King Young Jo. 6. Even though he was influenced by "Yi-Hak-Yip-Mun", "Xiao Er Yao Zheag Zhi Jue", and "Dong eui bo gam", his own explanation is clearer in his book called "Gup Yu Bang" which is based on his own clinical experiences. 7. "Gup Yu Bang" is a practical and experiential book.

• 전기의세계
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• v.32 no.11
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• pp.664-669
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• 1983

대규모 system을 취급하는 공학분야에 있어서 Matrix의 demension이 커짐에 따라 전산부담을 줄이기 위한 소요기억용량 절감 및 계산시간의 단축이 주된 관심사 중의 하나가 되어 왔다. LP(Linear Programming)기법 역시 대규모 선형 system에 적용될 경우 Basic Matrix 에 대한 dimensionality 문제를 포함하고 있으며 이에 대한 효과적인 해결방법으로 Bartels-Golub Decomposition 방법이 연구되어 있다. 이 방법은 Chan과 Yip등이 LSGA(Load Shedding and Generator Rescheduling) 문제에의 적용을 시도한 바 있으며, 일반적인 선형최적화 문제뿐만 아니라, dimensionality가 큰 Matrix의 Inverse 계산을 요하는 문제에 널리 적용될 수 있으므로 그 개요를 소개하고자 한다.

• Child Studies in Asia-Pacific Contexts
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• v.2 no.2
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• pp.123-132
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• 2012

The current study examined 3- and 4-year-olds' affective decision-making in a variety of cultural contexts by comparing European Canadian children to Chinese Canadian, Hong Kong Chinese, and mainland Chinese children (N = 245). All children were tested with a delay of gratification task in which children chose between an immediate reward of lower value and a delayed reward of higher value. Results showed that Chinese Canadian and Hong Kong Chinese children chose more delayed rewards than European Canadian children, with mainland Chinese children showing a trend toward more delayed rewards. Across cultures, 4-year-olds chose more delayed rewards than 3-year-olds; and among 4-year-olds, girls made more such choices than boys. The findings are consistent with previous findings that exposure to Chinese culture is associated with better cool executive function, but they also highlight the importance of examining development across diverse cultural contexts.

• Nuclear Engineering and Technology
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• v.41 no.1
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• pp.21-38
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• 2009

The use of multi scale modeling concepts and simulation techniques to study the destabilization of an ultrathin layer of oxide interface between a metal substrate and the surrounding environment is considered. Of particular interest are chemo-mechanical behavior of this interface in the context of a molecular-level description of stress corrosion cracking. Motivated by our previous molecular dynamics simulations of unit processes in materials strength and toughness, we examine the challenges of dealing with chemical reactivity on an equal footing with mechanical deformation, (a) understanding electron transfer processes using first-principles methods, (b) modeling cation transport and associated charged defect migration kinetics, and (c) simulation of pit nucleation and intergranular deformation to initiate the breakdown of the oxide interlayer. These problems illustrate a level of multi-scale complexity that would be practically impossible to attack by other means; they also point to a perspective framework that could guide future research in the broad computational science community.

• Microbiology and Biotechnology Letters
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• v.21 no.3
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• pp.247-255
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• 1993

Antitumor activity of Lactobacillus casei YIP 9018(LC9018) was studied in mice by using sarcoma 180(S-180) and Lewis lung carcinoma (3LL). Following the eatablishment of in vivo tumor models such as ascites form S-180, solid form S-180 and 3LL for estimating antitumor activity of Lactobacilli, optimal dose and injection route of heat-killed LC9018 for supperssion of local tumor were examined. Administration of 100ng/mouse of LC9018 significantly inhibited the growth of ascites form S-180, solid form S-180 and 3LL.