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Determination of Cholinesterase Activities in Sera of Human and Different Species of Animals by Means of the Propionylthiocholine-Dithiobis Procedure (Propionylthiocholine-Dithiobis 방법에 의한 사람과 몇몇 동물 혈청내의 Cholinesterase 활성도 측정)

  • Kim, Soon-tae;Kim, Sung-hoon;Do, Jae-cheul;Lee, Young-ho;Mo, Ki-cheul;Huh, Rhin-sou
    • Korean Journal of Veterinary Research
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    • v.27 no.1
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    • pp.153-155
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    • 1987
  • Determination of cholinesterase activity is a routine practice in many laboratories to detect the influence of cholinesterase inhibiting drugs such as organophosphate and carbamate insecticides. Among many different methods to determine the cholinesterase activity, the present method was the most recent, simple and accurate one for routine test in clinics. The results obtained in sera of human and the different species of animals by means of the present method were as follows: $5.76{\pm}1.12U/ml$ in human, $3.37{\pm}0.83U/ml$ in german shepherd, $0.61{\pm}0.18U/ml$ in rat, $14.91{\pm}3.10U/ml$ in mouse, $1.55{\pm}0.51U/ml$ in chicken, $0.28{\pm}0.11U/ml$ in slaughtered cattle and $0.50{\pm}0.10U/ml$ in slaughtered pig.

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Improvement of Nutritive Value and In vitro Ruminal Fermentation of Leucaena Silage by Molasses and Urea Supplementation

  • Phesatcha, K.;Wanapat, M.
    • Asian-Australasian Journal of Animal Sciences
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    • v.29 no.8
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    • pp.1136-1144
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    • 2016
  • Leucaena silage was supplemented with different levels of molasses and urea to study its nutritive value and in vitro rumen fermentation efficiency. The ensiling study was randomly assigned according to a $3{\times}3$ factorial arrangement in which the first factor was molasses (M) supplement at 0%, 1%, and 2% of crop dry matter (DM) and the second was urea (U) supplement as 0%, 0.5%, and 1% of the crop DM, respectively. After 28 days of ensiling, the silage samples were collected and analyzed for chemical composition. All the nine Leucaena silages were kept for study of rumen fermentation efficiency using in vitro gas production techniques. The present result shows that supplementation of U or M did not affect DM, organic matter, neutral detergent fiber, and acid detergent fiber content in the silage. However, increasing level of U supplementation increased crude protein content while M level did not show any effect. Moreover, the combination of U and M supplement decreased the content of mimosine concentration especially with M2U1 (molasses 2% and urea 1%) silage. The result of the in vitro study shows that gas production kinetics, cumulation gas at 96 h and in vitro true digestibility increased with the increasing level of U and M supplementation especially in the combination treatments. Supplementation of M and U resulted in increasing propionic acid and total volatile fatty acid whereas, acetic acid, butyric acid concentrations and methane production were not changed. In addition, increasing U level supplementation increased $NH_3$-N concentration. Result from real-time polymerase chain reaction revealed a significant effect on total bacteria, whereas F. succinogenes and R. flavefaciens population while R. albus was not affected by the M and U supplementation. Based on this study, it could be concluded that M and urea U supplementation could improve the nutritive value of Leucaena silage and enhance in vitro rumen fermentation efficiency. This study also suggested that the combination use of M and U supplementation level was at 2% and 1%, respectively.

A Study on the Improvement Plans of Disaster Response Capabilities for U-city (U-City 재난 대응역량 제고방안에 관한 연구)

  • Park, Hee-Soon;Lee, Jeong-Il;Yoon, Hyung-Goog
    • Journal of the Korean Society of Hazard Mitigation
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    • v.10 no.5
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    • pp.55-66
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    • 2010
  • As people's living standards and expectations for life increase, by applying advanced technology in the city, to enhance the convenience and safety needs are being expanded gradually. Accordingly, so the future of the city to see a viable alternative to that the ubiquitous city fused information communication technologies, large-scale projects conducted at the national level. Sectoral Disaster Prevention is ready for sure how much the lack of response capabilities in the field for checking solution has been suggested for The National Emergency Management Agency. These results present a Sectoral Disaster Prevention response capabilities somewhat different in were identified. Through these are proposed policy recommendations about Sectoral Disaster Prevention and insufficient areas of step response capabilities and building fire protection system approach combines ubiquitous technology in the new paradigm rather than traditional disaster management, greet era of ubiquitous.

The Wearing Sensation and Physiological Responses in School Wear in the High School Girl's (여고생 통학복의 착용감과 생리반응에 관한 연구)

  • Kweon, Soo-Ae
    • Korean Journal of Human Ecology
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    • v.7 no.2
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    • pp.81-91
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    • 1998
  • The subjects in this research were 368 girls in high school for survey, and wearing sensation and physiological responses were investigated through wearing trials on human body in climatic chamber based on these results from the survey. The results are as follows : 1. They enjoyed wearing t-shirts, jackets, vests, and blouses in order for the upper clothes, and they preferred t-shirts to blouses. For the lower clothes, they enjoyed slacks much more than skirts. The weight of clothes was significantly heavier in the group where they wore the uniforms(U-group) than in the group where they wore the free styles(F-group). When they chose the school wear, activity was the most important of all, and the maintenance was the least. 2. As the classes were a little cool and dry, most of them dissatisfied the environment. The degree of the satisfaction of the class environment and properties to it were higher in the U-group than in F-group. 3. In the textiles, colors, styles, activity, static electricity, seasonal property, and easiness of putting on and taking off the clothes, F-group was more satisfied than U-group. U-group was more satisfied than F-group in the soil of the clothes. 4. The thermal comfort, thickness, and tightness of the clothes were not significantly different between the groups. The clothes of U-group was heavier than those of F-group, and the tactile sensation in U-group was worse than F-group. In U-group the students felt the skirts very inconvenient when they acted. 5. The weight of the clothes influenced the wearing sensation, therefore the heavier the clothes were the less satisfied they felt. 6. The inside temperature of clothes was significantly higher in U-group than in F-group. The skin temperatures of abdomen and arm were significantly higher in U-group than in F-group, while the skin temperatures of thighs and legs were significantly lower in U-group than in F-group. U-group felt heavier than F-group in wearing the clothes. Therefore the improvement of the clothes weight is needed.

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Unaided Stapling Technique for Pure Single-Incision Distal Gastrectomy in Early Gastric Cancer: Unaided Delta-Shaped Anastomosis and Uncut Roux-en-Y Anastomosis

  • Suh, Yun-Suhk;Park, Ji-Ho;Kim, Tae Han;Huh, Yeon-Ju;Son, Young Gil;Yang, Jun-Young;Kong, Seong-Ho;Lee, Hyuk-Joon;Yang, Han-Kwang
    • Journal of Gastric Cancer
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    • v.15 no.2
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    • pp.105-112
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    • 2015
  • Purpose: Intracorporeal anastomosis is the most difficult procedure during pure single-incision distal gastrectomy (SIDG) that affects its generalization. We introduced unaided delta-shaped anastomosis (uDelta), a novel anastomosis technique, for gastroduodenostomy after pure SIDG, and compared the results with those of previously reported Roux-en-Y anastomosis (RY). Materials and Methods: Between March 2014 and March 2015, SIDG with D1+ lymph node dissection was performed for early gastric cancer through a 2.5-cm transumbilical incision without any additional port. uDelta was performed by the operator alone, without any intracorporeal assistance. Results: uDelta was performed on 11 patents, and uncut RY was performed on 5-patients without open or multiport conversion. R0 resection was performed in all cases. No significant differences were observed in mean age and body mass index between patients who underwent uDelta or RY. Mean operation times were $214.5{\pm}36.2$ minutes for uDelta and $240.8{\pm}65.9$ minutes for RY, which was not significantly different. Reconstruction time for uDelta was shorter than that for RY, with marginal statistical significance ($26.1{\pm}8.3$ minutes vs. $38.0{\pm}9.1$ minutes, P=0.05). There were no intraoperative transfusions, 30-day mortality, or anastomosis-related complications in either group. Average length of hospital stay was $8.2{\pm}1.9$ days in the uDelta group and $7.2{\pm}0.8$ days in the RY group (P=0.320). Conclusions: After carefully considering indications, uDelta can be a feasible and can be a reproducible reconstruction method after SIDG in early gastric cancer.

The Linear Thermal Expansion Measurements and Estimations for UO2 and (U1-yCey)O2 Pellet (UO2 및 (U1-yCey)O2 소결체의 열팽창 측정 및 평가)

  • Kim, Dong-Joo;Kim, Yong-Soo;Lee, Young-Woo
    • Journal of the Korean Ceramic Society
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    • v.42 no.5 s.276
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    • pp.346-351
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    • 2005
  • The linear thermal expansions of $UO_2$ and $(U_{1-y}Ce_y)O_2$ pellet were measured from room temperature to $1400^{\circ}C$ as a function of Ce contents (0, 7.63, 14.84, and $21.68 mol\%$) by using the TMA(Thermo-Mechanical Analysis) method. From the measured data, the linear thermal expansion rate, the coefficient of linear thermal expansion and density variation with temperature were calculated, and the best-fitted temperature-dependent equations were recommended. It was shown that the rate and coefficient of $(U_{1-y}Ce_y)O_2$ thermal expansion increased and the density decreased with increasing Ce contents.

The Effects of Swim Training on Neurogenesis in the Hippocampal Dentate Gyrus and Functional Ability After Focal Ischemic Stroke in Rats (수영 훈련이 뇌허혈 유발 흰쥐의 해마 치아이랑에서 뇌신경생성과 기능적 능력에 미치는 영향)

  • Kim, Ho-Sung;Kim, Deuk-Ho;Lee, Jeong-Pil;Kim, Young-Joo;Shin, Young-Oh;Kim, Sang-Hoon;Kwon, Ki-Wook;Oh, Jae-Keun
    • Physical Therapy Korea
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    • v.12 no.3
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    • pp.11-21
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    • 2005
  • The present study was aimed at investigating the effect of swimming training on brain function after focal cerebral ischemia in rats. Therefore, this study was examined on neurogenesis in dentate gyrus of hippocampus using 5-bromo-2'-deoxyuridine (BrdU) to label proliferating cells and assessed the neurological response following focal cerebral ischemia in rats using neurological motor behavioral test. In an observer-blinded fashion, twenty male Sprague-Dawley (280~310 g, 7 weeks old) rats were divided into four groups: MCAO plus swimming group (ME, $n_1$=5), MCAO plus control group (MC, $n_2$=5), SHAM plus swimming group (SE, $n_3$=5), SHAM plus control group (SC, $n_4$=5). The results of this study were as follows: 1) The limb placing time before and after swimming in the ME group were significantly longer than the MC group (p<.05), the SE group were significantly longer than the SC group (p<.01). 2) The balance beam scores before and after swimming in the ME group was higher than the SE group, the MC group was higher than the SC group but was not significantly different (p>.001). 3) The foot fault index before and after swimming training in ME group was significantly lower (i.e., improved) than the MC group (p<.001) and the SE group (p<.001), the SE group was significantly lower (i.e., improved) than the SC group (p<.001). 4) The mean number of BrdU-positive cells in the dentate gyrus in the ME group was significantly higher than the MC group (p<.001) and the SE group (p<.01). The MC group and the SE group was significantly higher than the SC group (p<.001). 5) There was significantly correlation between limb placing time and number of BrdU-positive cells on swimming training, there was positive correlation (r=.807, p<.0001) and between foot fault index and BrdU-positive cells number, there was negative correlation (r=-.503, p<.05). However, between balance beam scores and BrdU-positive cells number, there was no correlation. In conclusion, the present study demonstrates that the role of swimming training improves behavioral motor function probably by enhancing cell proliferation in that hippocampus. This study provides a model for investigating the stroke rehabilitation that underlies neurogenesis and functional ability.

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Development of Slow-Release Compound Fertilizer Used Urea-Resin for Upland Crop I. Manufacture of Slow-Release Compound Fertilizer (요소수지(尿素樹脂)를 이용(利用)한 전작용(田作用) 완효성(緩效性) 복비(複肥) 개발(開發) I. 제조시험(製造試驗))

  • Seong, Ki-Seog;Kim, Bok-Jin;Shin, Jae-Sung
    • Korean Journal of Soil Science and Fertilizer
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    • v.23 no.3
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    • pp.193-198
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    • 1990
  • In order to develop the slow-release compound fertilizer fur upland crops, trial products were made with ammonium sulfate, diammonium phosphate, and potassium chloride with filler of calcium sulfate, and hinder of additional material of urea resin Nine trial slow-release compound fertilizers were manufactured by varying urea/formaldehyde mole ratios(U/F mole ratio : 0.5, 1.0, and 1.5 ) and the proportions of urea resin (3.3. 6.7. and 10%) and the proportions of urea resin (3.3. 6.7 and 10%). The aimed percentage of $N-P_2O_5-K_2O$ in the trial product was 12.5-10.0-14.0%, which was appropriate for Chinese cabbage. The N dissolution of the trial products in water was slower with lower U/F ratio and in creased to the proportion of urea resin. The dissolved nitrogen in water after 24hrs ranged from 69 to 83 percent of total nitrogen in the trial products.

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Effect of Superovulation and Freezing Methods on the Survival of Bovine Embryos (육우의 과배란 유기와 동결방법이 난자의 생존율에 미치는 영향)

  • 문성호;김중계
    • Journal of Embryo Transfer
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    • v.11 no.3
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    • pp.271-281
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    • 1996
  • The effect of superovulation (PMSG, FSH) on the ovarian response of matured cows were tested. The survival rates of bovine embryos and ovarian oocytes frozen by slow, rapid freezing and vitrification were investigated. A total of 15 heads of cow were devided into 3 groups by injection dose of GTH (PSMG, FSH). Each group was superrovulated with injections of 2, 500, 3, OOOJU PSMG and 40mg FSH followed by injection of 30mg PGF2a. Embryos were non-surgically recovered from superovulated cows 6~7days after estrus. The recovered embryos were frozen in 10% glycerol + 10% sucrose by slow and rapid freezing. Ovarian oncytes were frozen in 20% g]ycerol+l0% ethylene glycerol + 30% Ficol + 10% sucrose by vitrification and the survival of frozen embryos and ovarian oncytes were judged by FDA-test. The results are summarized as follows; 1. Estrus after the injection of 2500, 3000 I.U. PMSG and 4Omg FSH were 32.8, 35.0 and 43.4 and the duration of estrus were 18.6, 18.8 and 22.4 hours respectively. 2. The average sizes of the left ovaries were 5.4cm (2, 500 IU PMSG), 5.1cm (3, OOOIU PMSG) and 6.4cm (FSH), and the right were 6.2cm (2, 5001U PMSG), 5.7cm (3, OOOIU PMSG) and 7.&m (FSH) respectively. There were significant differences in the right overies among treatments (P<0.05). 3. The average number of ovarian follicles in the left ovaries were 4.8 (2, 500 IU PMSG), 5.2(3, 000 IU PMSG) and 7.8 (FSH) respectively. There were significant difference in the right ovaries among treatments (P<0.05). 4. In the average numbers of ovulation points in the left ovaries were 3.0 (2, 5001U PMSG), 3.2 (3, OOOIU PMSG) and 4.4 (FSH) respectively, and the right were 7.2 (2, 5001U PMSG), 7.8(3, 000IU PMSG) and 11.4 (FSH). There were significant differences in the right ovaries among treatments (P<0.05). 5. The numbers of the recovered embryos were 20 (2, 5001U PMSG), 19 (3, 000 IU PMSG) and 21 (FSH) respectively, and oncytes and degenerted oncytes were 6.5 and 11.0 Estrus periods of post parturation were 52.4days (2, 5001U PMSG), 69.8days (3, OOOIU PMSG) and 62.4days (FSH) respectively. 6. The FDA score of cow morulae frozen by slow freezing, sernirapid frezing and vitrified freezing was higher in slow (3.1) and vitrified freezing (3.0) than that in semirapid freezing (1.28). The FDA-scores of cow, pig and rabbit ovarian oocytes frozen in 20% glycerol + 10% ethylene glycol + 30% Ficoll + 10% sucrose by vitrification were higher in cows (3.3) than both in pigs (2.6) and rabbits (2.3).

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Study on the Characteristics of Laser-induced Fluorescence from Trace Samarium, Europium and Terbium (미량분석을 위한 Sm, Eu과 Tb의 레이저 여기 형광 특성 분석)

  • Lee, Sang-Mock;Shin, Jang-Soo;Zee, Kwang-Yong;Kim, Cheol-Jung
    • Nuclear Engineering and Technology
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    • v.21 no.4
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    • pp.287-293
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    • 1989
  • The purpose of this study was to develop a rapid and effective method of laser-induced fluorescence analysis for thrace amounts of Sm, Eu and Tb in nuclear fuels. The features of the method are the use of the distinct fluorescence wavelengths and the discriminative lifetimes of the respective elements when excited by a pulsed nitrogen laser. Fluorescence signals of the three elements were isolated by adequate selection of the filters or complexing agents (HFA, TTA) or discriminative delay and gate times in the signal processing circuit. It was found that S $m^{+3}$ and E $u^{+3}$ emitted strong fluorescence in the two complexing agent solutions or HFA and TTA. But in the case or T $b^{+3}$, the fluorescence signal was detected only in HFA solution. With respect to the concentrations of S $m^{+3}$, E $u^{+3}$ and T $b^{+3}$, the fluorescence signal intensities gave superior linearities in the range of 5 ppb-10 ppm for S $m^{+3}$, 0.5 ppb-1 ppm for E $u^{+3}$, and 0.1 ppb-300 ppb for T $b^{+3}$, The detection limits obtained were 5 ppb for S $m^{+3}$, 0.1 ppb for E $u^{+3}$, and 0.01 ppb for T $b^{+3}$, respectively.

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