• 한국산업보건학회지
• /
• 제4권2호
• /
• pp.189-197
• /
• 1994

Analytic methods for Cr(VI) level in industrial hygienic field were suggested by the National Institute for Occupational Safety and Health(NIOSH method 7600, 7604). There were growing needs for measurement of Cr(III) and Cr(VI) levels simultaneously. Two analytical methods were suggested to determine Cr(III) and Cr(VI) levels simultaneously. The one is method by using reversed phase high peformance liquid chromatography(HPLC) and the other is by using ion exchange HPLC. The purpose of this work was to evaluate the usefulness of these two analytic methods. For the difference of ionic charges of Cr(III)-ethylendiamine tetraacetic acid(EDTA) chelate and $CrO_4{^-2}$, we could detect them simultaneously by ion exchange HPLC. Also, we attempted to determine the levels of Cr(III) and Cr(VI) chelated with sodium diethyldithiocarbamate(NaDDTC) by using reversed phase HPLC. The confirmation of Cr(III) and Cr(VI) were checked by fraction collector and nameless atomic absorption spectrometer. The optimal conditions for the formation of Cr(III)-EDTA chelate were two hours incubation period with pH 5. Cr(III)-EDTA and Cr(VI) in EDTA solution were successfully separated by anion exchange column using $Na_2CO_3/NaOH$ mixture as mobile phase. Peaks of Cr(III)-EDTA and Cr(VI) in EDTA were identified at 5 minutes and 7 minutes of retention time respectively by the ion exchange HPLC. The formation of Cr(III)-NaDDTC and Cr(VI)-NaDDTC chelates were twelve hours incubation period. Cr(III)-NaDDTC and Cr(VI)-NaDDTC chelates were separated by reversed phase column using methanol and water mixture as mobile phase. Peaks of Cr(VI)NaDDTC and Cr(III)-NaDDTC chelates were identified at 13 minutes and 26 minutes of retention time respectively by the reversed phase HPLC. Due to reduction of Cr(VI) to Cr(III), it seems to be not suitable for simultaneous determination of Cr(III)-NaDDTC and Cr(VI)-NaDDTC chelates by reversed phase HPLS. Simultaneos determination of Cr(III) and Cr(VI) by ion exchange HPLC was more accurate and simple method.

• 대한치과기공학회지
• /
• 제29권2호
• /
• pp.197-211
• /
• 2007

With the development of orthodontics and increasing concerns on physical appearance, the number of patients has been steadily increasing. It is quite important not only to make effective cure plans and accurate diagnoses but also to have a thorough grasp of patients' malocclusion types and their occurrence frequency, in addition to patients' personality in order to cure the patients appropriately. This study is based on 946 malocclusion patients who had visited Chong-A Dental Hospital from 1999 to 2004 and investigated their aspects of malocclusion and characteristics of their gender, age and residence. The results are as follows. 1. The number of patients per year had been decreased until 2001, after which year the number had fluctuated. The number was the largest in 1999, 169 and the smallest in 2001, 140. Female occupied 68.0% of the total, twice as many as male, 32.0%) 2. Based on the Angle's classification, 19 or over year - old group was the largest of the total, 59.3% and 6 or younger year - old group, the smallest, 0.5%. The 19 or over year old group was less than a half of the total (47.4%) in 2003 and there were no patients who belonged to the 6 or younger year - old group in 2003 and 2004. 3. Distributions on the types of malocclusion have shown that 39.9 % of the total are in the Class I, the largest, 31.0% in the Class I and 29.2 in the Class II, the smallest. 1) The number of the ClassI was 73, the largest, that of the Class III being 35, the smallest in 1999. On the whole, the number of the Class I accounted for the largest part of the total. 2) The number of male patients in the Class II was the smallest, generally being the largest in the Class I. In case of female, that of the Class III was the smallest. 3) Based on the age, the Class I was the highest in between 7 and 13 age group, the Class III the lowest. The Class I occupied the largest around 40%. 4) In the shape of physiognomy, the meso occupied the largest part among all the Class, of which the Class II was the highest, 64.2%. The bracy was the largest in the Class I, and the dolicho in the Class III. 5) In the profile, the convex shape was the largest in the Class I and II, and especially in the Class II, over 3/4 of the total, 75.4%. In contrast, the direct shape was the largest in the Class III and the sunken shape occupied 33.3%, which was nearly ten times more than the case of the Class I and III. 6) In the asymmetry of physiognomy, the number of patients of the Class IIIwas the largest, 34.1% and that of the Class II, the smallest, 19.5%. It was found that about one fourth of the malocclusion patients were under the asymmetry of physiognomy. 4. In the distribution of patients' residence, 81.4% were from the Seoul Metropolis and 48.2% from Gangnam-Gu where Chong-A Dental Hospital is located and Seocho-Gu and Songpa-Gu which are adjacent to Gangnam-Gu.

• 대한의생명과학회지
• /
• 제13권3호
• /
• pp.197-206
• /
• 2007

P2X receptors are membrane-bound ion channels that conduct $Na^+,\;K^+$, and $Ca^{2+}$ in response to ATP and its analogs. There are seven subunits identified so far ($P2X_1-P2X_7$). $P2X_2$ receptors are known to be expressed in a wide range of organs including brains and adrenal grands. PC12 cells are originated from adrenal grand and differentiated by nerve growth factor or pituitary adenylate cyclase activating poly peptide (PACAP). Previous studies indicate that $P2X_2$ receptor activation in PC12 cells couples to $Ca^{2+}-dependent$ release of catecholamine and ATP. It is known that acidic pH potentiates ATP currents at $P2X_2$ receptors. This leads to a hypothesis that $P2X_2$ receptors may play an important role in PC12 cell differentiation, one of the characteristics of which is neurite outgrowth, induced by the hormones under lower pH. In the present study, we isolated several clones which potentiate neurite outgrowth by PACAP in acidic pH (6.8), but not in alkaline pH (7.6). RT-PCR and electrophysiology data indicate that these clones express only functional $P2X_2$ receptors in the absence or presence of PACAP for 3 days. Potentiation of neurite outgrowth resulted from PACAP (100 nM) in acidic pH is inhibited by the two P2X receptor antagonists, suramin and PPADS ($100\;{\mu}M)$ each), and exogenous exprerssion of ATP-binding mutant $P2X_2$ receptor subunit ($P2X_2[K69A]$). However, acid sensing ion channels (ASICs) are not involved in PACAP-induced neurite outgrowth potentiation in lower pH since treatments of an inhibitor of ASICs, amyloride ($10\;{\mu}M$), did not give any effects to neurite extension. The vesicular proton pump ($H^+-ATPase$) inhibitor, bafilomycin (100 nM), reduced neurite extension indicating that ATP release resulted from $P2X_2$ receptor activation in PC12 cells is needed for neurite outgrowth. These were confirmed by activation of mitogen activated protein kinases, such as ERKs and p38. These results suggest roles of ATP and $P2X_2$ receptors in hormone-induced cell differentiation or neuronal synaptogenesis in local acidic environments.

• 대한지역사회영양학회지
• /
• 제21권3호
• /
• pp.284-292
• /
• 2016

Objectives: This study compared the differences of postmenopausal women's bone mineral density in relation to the degree of obesity, metabolism index and dietary factors that affect bone mineral density. Methods: The subjects included in the study are 39 postmenopausal women of normal weight with body mass index less than $25kg/m^2$ and 32 postmenopausal who are obese. Anthropometry and biochemical analysis were performed and nutrient intakes and DQI-I were assessed. Results: Normal weight women were $56.03{\pm}3.76years$ old and obese women were $58.09{\pm}5.13years$ old and there was no significant difference in age between the two groups. The T-score of bone mineral density was $0.03{\pm}1.06$ in normal weight women and $-0.60{\pm}1.47$ in obese women and this was significantly different between the two groups (p<0.05). Blood Leptin concentration was significantly lower in normal weight women ($6.09{\pm}3.37ng/mL$) compared to obese women in ($9.01{\pm}4.99ng/mL$) (p<0.05). The total score of diet quality index-international was $70.41{\pm}9.34$ in normal weight women and $64.93{\pm}7.82$ in obese women (p<0.05). T-score of bone mineral density showed negative correlations with percentage of body fat (r = -0.233, p=0.05), BMI (r = -0.197, p=0.017), triglyceride (r = -0.281, p=0.020) and leptin (r = -0.308, p=0.011). The results of multiple regression analysis performed as the method of entry showed that with 22.0% of explanation power, percentage of body fat (${\beta}=-0.048$, p<0.05), triglyceride (${\beta}=-0.005$, p<0.05) and HDL-cholesterol (${\beta}=0.034$, p<0.01), moderation of DQI-I (${\beta}=-0.231$, p<0.05) affected T-score significantly. Conclusions: The results of the study showed that obese women have less bone density than those with normal weight women. In addition, the factor analysis result that affect bone mineral density showed that intake of fat is a very important factor. Therefore, postmenopausal women need to maintain normal weight and manage blood lipid levels within normal range. They also need to take various sources of protein and reduce consumption of empty calorie foods that have high calories, fat, cholesterol and sodium.

• Toxicological Research
• /
• 제34권3호
• /
• pp.191-197
• /
• 2018

In 2015, a candidate for the second national reference standard (NRS) of Gloydius snake venom was produced to replace the first NRS of Gloydius snake venom. In the present study, the potencies of the candidate were determined by a collaborative study, and the qualification of the candidate was estimated. The potencies of the candidate were determined by measuring the murine lethal titers and lapine hemorrhagic titers of venom against the regional working reference standard (RWRS) for antivenom using the methods described in the previous report for the first NRS of Gloydius snake venom. Three Korean facilities contributed data from a total of 30 independent assays. Subsequently, two foreign national control research laboratories contributed to this collaborative study. The results were calculated using the Reed-Muench method for lethality and determined using a mixed-effects model for hemorrhage. The general common potencies of the lethal and hemorrhagic titers were obtained from the results of the 30 tests performed at three Korean facilities. The results are expressed in micrograms for 1 test dose (TD) with a 95% confidence interval as follows: a lethal titer of $90.13{\mu}g/TD$ (95% confidence interval = $87.39{\sim}92.86{\mu}g$) and a hemorrhagic titer of $10.80{\mu}g/TD$ (95% confidence interval = $10.46{\sim}11.14{\mu}g$). In addition, the candidate preparation showed good quality evaluation according to the results of the quality estimation of the candidate and is judged to be suitable to serve as the Korean NRS for snake venom. In conclusion, the second NRS of Gloydius snake venom was established in this study and will be used for national quality control, including a national lot release test of Korean antivenom products.

• 한국가금학회지
• /
• 제13권2호
• /
• pp.197-208
• /
• 1986

본 시험은 6가지 다른 사양방법이 White Plymouth Rock 육용종 웅계의 생체중, 정소, 계관 그리고 뇌하수체의 발육 및 성성숙에 미치는 영향을 구명하기 위하여 부화후부터 22주령까지 2주간격으로 생체중, 정소, 계관 그리고 뇌하수체의 중량이 조사되었으며, 또한 정소조직과 정액성상이 조사되었다. 그 얻어진 결과를 요약하면 다음과 같다. 1. 생체중, 정소, 계관의 발육은 10주령 이후부터 무제한급이군들이 제한급이군들(무제한급이군들의 70%급이)보다 2주 더 빠름을 나타내었다. 정소 및 계관의 급증시기는 무제한급이군들에서 20주령이었고, 제한급이군들에서 22주령이었다. 2. 뇌하수체의 무게는 부화후부터 22주령까지 4주령, 14주령, 16주령을 제외하고 각 주령별, 각 처리군간에 유의성이 나타나지 않았다. 뇌하수체의 중량은 주령이 경과하면서 완만한 증가를 나타내었다. 3. 22주령까지에서 주령, 생체중, 정소, 계관 및 뇌하수체간에는 고도의 유의적인 상관관계를 나타내었다. 4. 세정관의 관경과 강경은 부화후부터 10주령까지 점진적으로 증가되다가 무제한 급이군들에서는 12주령부터 제한급이군들에서는 14주령부터 급증하기 시작하였고, 그 후에도 22주령까지 계속증가되었다.

• 자원환경지질
• /
• 제30권3호
• /
• pp.197-207
• /
• 1997

Rocks in the Cheongsong mine area consist of Precambrian gneiss, Cretaceous sedimentry rocks and late Cretaceous quartz porphyry. The Cheongsong deposit is composed of many hydrothermal quartz veins of strikes $N30^{\circ}{\sim}60^{\circ}W$, dips $60{\sim}85^{\circ}E$ which fill WNW fault system. Pyrite and hematite occur within transparent quartz near margins of early stage II, and milky quartz of middle stage II coexists with sphalerite, chalcopyrite and galena coexisting with Cu-Pb-Bi minerals in center part of stage II quartz veins. Stage III calcite vein filled cracks or fractures of earlier quartz veins contains native copper and chalcopyrite. Supergene minerals are chalcocite, covellite, malanchite and chrysocolla. Alteration minerals are sericite, chlorite, argillite, epitode and pyrite. Ranges of salinities and homogenization temperatures for fluid inclusions in the individual periods of stage II are: 3.7 to 7.8 wt.% eq. NaCl and 200 to $380^{\circ}C$ in transparent quartz of early stage II; 0.7 to 6.4 wt.% eq. NaCl and 200 to $320^{\circ}C$ in milky quartz of middle stage II; 0.0 to 0.9 wt.% eq. NaCl and 250 to $320^{\circ}C$ in calcite of late stage II. Those of stage III calcite range about 0 wt.% eq. NaCl, and from 140 to $260^{\circ}C$, respectively. The relationship between salinities and temperatures shows decrease tendency with paragenetic time from stage II to III. The ${\delta}^{18}O_{H_2O}$ value is 0.5‰ in stage I, range from 0.5 to -0.4‰ in stage II, and from -3.2 to -3.7‰ in stage III. Calcite in the stage II and stage III has ${\delta}^{13}C$ values of -5.0‰ and -4.5 to -4.9‰, respectively. There is a decrease in sulfur fugacity values with paragenetic time of stage II, from $10^{-6.3}$ atm for early mineralization, to $10^{-6.5}$ atm for middle stage, to $10^{-8.0}$ atm for late mineralization of stage II. The results of stable isotope and fluid inclusion indicate that ore fluids reacted with meteoric water and wall rock in the Cheongsong hydrothermal system.

• 환경생물
• /
• 제27권2호
• /
• pp.216-229
• /
• 2009

금강 수계에 위치한 구룡산, 전월산, 사마산, 용머리산, 함라산의 관속식물상을 파악하고 보존대책을 제시하기 위하여 자생식물에 대한 방문조사를 실시하였다. 조사는 2007년 5월부터 2008년 4월까지 17회 수행하였으며, 조사지역 내에 자생하는 식물을 채집 동정하고 목록을 작성하였다. 목록을 바탕으로 한국특산종 및 법적보호종(멸종위기종, 보호종, 천연기념물)의 분포 현황 및 생태계 위협종 등의 분포 상황을 파악하였다. 조사지역 중 구룡산의 관속식물은 70과 151속 158종 22변종 3품종의 총 181분류군, 전월산에서는 45과 78속 72종 10변종의 총 92분류군, 사마산에서는 35과 69속 67종 10변종의 총 77분류군, 용머리산에서는 57과 109속 123종 13변종 1품종의 총 137분류군, 함라산에서는 64과 155속 178종 19변종 1품종의 총 197분류군으로 조사되었다. 이를 합친 5개 조사구역의 관속식물은 86과 246속 297종 34변종 3품종의 총 334분류군으로 판명되었다. 한국 특산종은 8분류군이 조사되었으나 희귀 및 멸종위기식물은 발견되지 않았다. 환경부 지정 식물구계학적 특정식물종은 4등급종 2분류군, 3등급종 8분류군이 확인되었다. 1, 2등급종을 포함하여 특정식물종은 모두 24분류군으로 확인되었다. 등산로 주변에 귀화식물 분포가 높았고 총 25분류군이 확인되었다. 금강 중류는 행정복합도시 건설로 인해 식생 파괴가 많이 우려되는 상황이었으며, 경작지 등의 개간에 의해 식물다양성이 많이 감소되어 있어 보존대책을 수립할 필요가 있는 것으로 사료된다.

• 농업과학연구
• /
• 제13권2호
• /
• pp.193-197
• /
• 1986

본(本) 시험(試驗)은 임신말기(姙娠末期) 및 초기포유기간(初期哺乳期間)의 모돈(母豚)에 아미노산 킬레이트 철분제(鐵分齊)를 급여(給與)함으로써 자돈(仔豚)에게 철분제(鐵分劑)를 경구투여(經口投與)하거나 주사(注射)하지 않고 자돈(仔豚)의 철분결핍성(鐵分缺乏性) 빈혈(貧血)을 예방(豫防)할 수 있는 지를 구명(究明)하기 위해서 모돈(母豚) 20두(頭)를 가지고 4처리(處理)로 나누어 실시(實施)한 바, 그 결과(結果)를 요약(要約)하면 다음과 같다. 1. 생시체중(生時體重)은 각(各) 처리군(處理群)들 간(間)에 차이(差異)가 없었으나, 15일령(日齡)의 체중(體重)에서는 킬레이트 철분급여군(鐵分給與群)이 제일 무거웠고, 35일령(日齡)의 체중(體重)에서는 대조군(對照群)이 제일 가벼웠다. 2. 이유시(離乳時) 생존율(生存率)은 각(各) 처리군(處理群)들 간(間)에 차이(差異)를 인정(認定)할 수 없었다. 3. 헤모그로빈, 적혈구(赤血球) 및 헤마토크리트의 수준(水準)은 출산시(出生時)와 15일령(日齡)에서 킬레이트 직분(織分) 급여군(給與群)이 대조군(對照群)에 의하여 높은 수준(水準)을 보였으며(p<.05) 35일령(日齡) 이유시(離乳時)에는 각(各) 처리군(處理群)들 간(間)에 차이(差異)가 없었다.

• Journal of Ginseng Research
• /
• 제40권3호
• /
• pp.203-210
• /
• 2016

Background: Korean Red Ginseng (KRG) is a well-known natural product with anticarcinogenic and antioxidant effects. We evaluated the antifatigue effect of KRG in patients with nonalcoholic fatty liver disease (NAFLD). Methods: Eighty patients with NAFLD were prospectively randomized to receive 3 wk of KRG or placebo in addition to counseling on healthy eating and regular exercise. Liver function test, proinflammatory cytokines, adiponectin, antioxidant activity, and fatigue score were measured and compared according to the body mass index between the KRG and placebo groups. Results: The liver function tests were significantly improved after 3 wk of treatment in both groups. The mean levels (at baseline and after treatment) of tumor necrosis factor-${\alpha}$ were $108.0pg/mL{\pm}54.8pg/mL$ and $92.7pg/mL{\pm}39.0pg/mL$ (p = 0.018) in the KRG group and $123.1pg/mL{\pm}42.1pg/mL$ and $127.5pg/mL{\pm}62.2pg/mL$ (p = 0.694) in the placebo group, respectively. There was a significant difference in change of adiponectin levels between the KRG ($7,751.2pg/mL{\pm}3,108.1pg/mL$ and $8,197.3pg/mL{\pm}2,714.5pg/mL$) and placebo groups ($7,711.6pg/mL{\pm}3,041.3pg/mL$ and $7,286.1pg/mL{\pm}5,188.7pg/mL$, p = 0.027). In patients with overweight, the fatigue score was significantly decreased in the KRG group ($35.0{\pm}13.2$ and $24.5{\pm}8.9$, p = 0.019). Conclusion: Our results show that KRG might be effective in reducing proinflammatory cytokine and fatigue in overweight patients with NAFLD, in addition to improvements in adiponectin levels.