• Preventive Nutrition and Food Science
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• 제4권1호
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• pp.23-27
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• 1999

To evaluate the quality of fish extracts with spicy vegetables (garlic, onion and ginger) in suppressing fishy oder, fish extracts of crucian carp, loach, bastard halibut and jacopever were processed at 100 $^{\circ}C$ for 6 hours, and their in vitro and in vivo protein qualities were determined . Protein and total lipid contents were closely related to the degree of discarding floated lipid on fish extracts and the kinds of added apicy vegetables . Boiling (10$0^{\circ}C$) , appeared to improve in vitro protein qualities slightly more than hydrocooking (11$0^{\circ}C$), but those with mild processing tended to result in better protein qualities than high temperature cooking (136-14$0^{\circ}C$). Spicy vegetables did not have remarkable effects on improving in vitro protein quality parameters. Fish extracts with 10% ginger were generally higher in in vitro protein quality than with the other vegetables . In spite of higher in vivo protein digestibility of fish extracts containing spicy vegetables processed under mild conditions(10$0^{\circ}C$), PERs of those extracts were not higher htan those of extranct processed at high temperature.

• Asian-Australasian Journal of Animal Sciences
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• 제3권1호
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• pp.39-46
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• 1990

This experiment was conducted to study the effects of blood-mixed and heat-treated protein feeds on protein degradation in the rumen, flow of protein to the abomasums and availability of undegraded protein in the intestine of sheep in a $4{\times}4$ Latin square design. Soybean oil meal, rapeseed meal, and whole soybean were mixed with fresh swine blood and dried at $140^{\circ}C$ for 2 h. Proportionate disappearance of apparently digested OM in the postrumen for the blood and heat treated protein group was ranged from 43.2 to 50.5% as compared with 28.0% for the unheated soybean oil meal diet. The treated protein supplements were resulted in greater total N and NAN flow passing at the abomasums than untreated soybean oil meal diet was fed. The quantities of undegraded feed N passing at the abomasums for the treated protein diets was approximately twice as high as that of the untreated soybean oil meal diet and the estimated amount of undegraded N of the protein supplement itself was 79.1 to 84.2% as compared with 15% of soybean oil meal.

• 한국식품과학회지
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• 제2권2호
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• pp.49-55
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• 1970

대두단백육(textured soybean protein food)의 제조 과정에서 분리한 대두단백의 건조공정은 단백 gel의 성질과 보수력, 점성도 같은 단백질 특성에 영향을 미쳤다. 모델시스템의 실험에서 질소용해계수로 표시한 대두단백의 변성도는 분리대두단백 gel의 강도의 중요한 변수로 나타났다. gel strength는 질소용해계수가 43이었을 때 최대가 되었고 질소 용해계수가 그보다 증가할 때나 감소할 때는 gel strength가 감소되었다. 이러한 사실은 분리대두단백의 건조공정 중 적당한 단백의 변성은 단백육제조에 필요하며 그 이상의 높은 질소용해계수를 갖도록 할 필요는 없다.

• Food Quality and Culture
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• 제3권1호
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• pp.45-48
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• 2009

The investigation assessed the influence of Compositae plants consumption on the protein profile in streptozotocin (STZ)-induced diabetic rats. Diabetes was induced in Sprague-Dawley rats by injection of STZ (45 mg/kg body weight) into tail vein. The rats were randomly assigned to five groups: normal and STZ-control fed an AIN-93 diet, and groups whose diets were supplemented with 10% Compositae powder containing Artemisia iwayomogi (A. iwayomogi), Atractylodes lancea (A. lancea) or Taraxacum mongolicum (T. mongolicum). To observe the effects of Compositae plants in the animal model, the levels of protein in liver, kidney, lung, pancreas, and brain were determined after 4 weeks. The level of protein in kidney increased significantly in rats receiving the A. iwayomogi- and T. mongolicum-supplemented diet compared to the STZ-control group. The level of protein in lung was increased significantly in the A. iwayomogi-supplemented group. Blood glucose level correlated well with brain protein level but did not correlate with other protein levels. Also, blood glucose correlated inversely with kidney, lung and brain protein levels. It is suggested that supplementation with A. iwayomogi in diabetic rats leads elevates protein in kidney and lung.

• 생명과학회지
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• 제8권2호
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• pp.158-161
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• 1998

Farmesy-Protein Transferase의 저해제는 Ras단백질의 발암활성을 차잔하는 항암제의 후보로서 알려져있다. 우리는 최근에 topoisomerase I에 대하여 저해활성을 갖는 dihydrotanshinone I을 약용식물인 Salvia miltorhiza Bunge(Danshen)으로부터 분리하였다. Dihydrotanshinone Iml 작용기작의 해석을 위한 시도에서 farmesy-Protein Transferase에 대한 저해능($IC_{50}$치= 15ug/ml)을 관찰하였으며, 이것은 유용한 항암제로서의 가능성을 제시한 결과로 본다.

• Animal cells and systems
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• 제1권1호
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• pp.135-142
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• 1997

Random sequencing of expressed sequence tags in roots of Chinese cabbage led to isolation of a partial cDNA clone, BR77, which encoded a putative protein kinase. Using the BR77 cDNA as a probe, we isolated a full-length cDNA encoding the Brassica campestris protein kinase 1 (Bcpk1). The Bcpt1 cDNA contained one open reading frame encoding a polypeptide of 439 amino acids. The putative polypeptide consisted of a short N-terminal region and a protein kinase catalytic domain. The catalytic domain of Bcpkl showed a high homology to cAMP- and calcium- phospholipid-dependent subfamilies of serine/threonine protein kineses. Eleven major catalytic domains in protein kineses were well conserved in Bcpk1. However, Bcpk1 contained a unique nonhomologous intervening sequence between subdomains VII and VIII, which was not found in protein kineses of animals and lower eukaryotes. Genomic DNA gel blot analysis showed that Bcpt1 genes might be present as three copies in the Chinese cabbage genome. These imply that Bcpk1 belongs to a plant-specific serine/threonine protein kinase subfamily.

• Journal of Microbiology
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• 제44권2호
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• pp.145-154
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• 2006

Heterotrimeric G proteins (G proteins) are conserved in all eukaryotes and are crucial components sensing and relaying external cues into the cells to elicit appropriate physiological and biochemical responses. Basic units of the heterotrimeric G protein signaling system include a G protein-coupled receptor (GPCR), a G protein composed of ${\alpha},\;{\beta},\;and\;{\gamma}$ subunits, and variety of effectors. Sequential sensitization and activation of these G protein elements translates external signals into gene expression changes, resulting in appropriate cellular behaviors. Regulators of G protein signaling (RGSs) constitute a crucial element of appropriate control of the intensity and duration of G protein signaling. For the past decade, G protein signaling and its regulation have been intensively studied in a number of model and/or pathogenic fungi and outcomes of the studies provided better understanding on the upstream regulation of vegetative growth, mating, development, virulence/pathogenicity establishment, and biosynthesis of secondary metabolites in fungi. This review focuses on the characteristics of the basic upstream G protein components and RGS proteins, and their roles controlling various aspects of biological processes in the model filamentous ascomycete fungus Aspergillus nidulans. In particular, their functions in controlling hyphal proliferation, asexual spore formation, sexual fruiting, and the mycotoxin sterigmatocystin production are discussed.

• 동아시아식생활학회지
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• 제7권2호
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• pp.153-158
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• 1997

This study was designed to find out the effects of soybean protein and milk protein between nondiabetic and diabetic rats. The experimental results are summarized as follows. 1. Total food intake was higher in diabetic soybean protein group than other groups but it was not significant. 2. The change of body weight was lower in diabetic soybean protein group than other groups and the soybean protein was effective to maintain the ideal body weight. 3. The effects of lowering total cholesterol and glucose in serum was higher in soybean protein groups than the milk protein groups.

• 미생물학회지
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• 제28권3호
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• pp.258-263
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• 1990

고도 카드뮴 내성 Hansenula anomale B-7으로부터 카드뮴 결합 단백질을 분리하여 전기 영동적으로 단일단백질로 정세됨을 확인했다. 이 단백질의 분자량은 약 33000으로서, 분자량 18000과 14000의 subunit로 구성되어 있다. 카드뮴 결합 단백질의 는 19.58이였으며, $100{\mu}{\textrm{g}}$의 단백질 중에 $9.26{\mu}{\textrm{g}}$ 카드뮴을 함유했다. 카드뮴 결합 단백질로부터 14종류의 아미노산이 검출되엇으며, aspartic acid, glycine과 alanine이 비교적 많이 함유되어 있으며, proline, valine 및 methionine은 검출되지 않았다. 본 연구에 사용된 카드뮴 내성효모로부터 분리, 정제된 카드뮴 결합 단백질은 cysteine과 카드뮴을 다량 함유하여 metallothionein의 특징을 잘 나타내고 있었다.

• 한국식품영양과학회지
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• 제22권6호
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• pp.758-762
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• 1993

Sodium hexametaphosphate(SHMP)가 탈지대두박 단백질의 추출과 농축단백질의 색도 및 아미노산 조성에 미치는 영향을 검토하였다. SHMP에 의한 단백질의 추출은 1.5% SHMP에서 가장 높았으며 이 이상의 농도에서는 오히려 감소하는 결과를 나타내었다. 또한 참깨박 단백질의 추출은 pH 5.0에서 17.0%, pH 2.0에서는 22.7%로 아주 낮았으나 pH 12.0에서 60.7%의 단백질이 추출되었다. 시료와 용매와의 비율에 있어서는 1 : 40에서 약 60%의 단백질이 추출되었으며 비율이 증가할수록 추출율이 증가하는 경향을 나타내었고 SHMP처리로 인해 농축단백질의 색도가 개선되었다. 참깨빡 농축단백질의 아미노산 조성을 비교한 결과 SHMP의 처리에 의해 lysine과 hethionine의 함량이 현저히 감소된 반면 valine과 leucine함량은 증가된 결과를 나타내었다.