• Journal of Ginseng Research
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• 제47권3호
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• pp.376-384
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• 2023

Background: Hepatic lipid disorder impaired mitochondrial homeostasis and intracellular redox balance, triggering development of non-alcohol fatty liver disease (NAFLD), while effective therapeutic approach remains inadequate. Ginsenosides Rc has been reported to maintain glucose balance in adipose tissue, while its role in regulating lipid metabolism remain vacant. Thus, we investigated the function and mechanism of ginsenosides Rc in defending high fat diet (HFD)-induced NAFLD. Methods: Mice primary hepatocytes (MPHs) challenged with oleic acid & palmitic acid were used to test the effects of ginsenosides Rc on intracellular lipid metabolism. RNAseq and molecular docking study were performed to explore potential targets of ginsenosides Rc in defending lipid deposition. Wild type and liver specific sirtuin 6 (SIRT6, 50721) deficient mice on HFD for 12 weeks were subjected to different dose of ginsenosides Rc to determine the function and detailed mechanism in vivo. Results: We identified ginsenosides Rc as a novel SIRT6 activator via increasing its expression and deacetylase activity. Ginsenosides Rc defends OA&PA-induced lipid deposition in MPHs and protects mice against HFD-induced metabolic disorder in dosage dependent manner. Ginsenosides Rc (20mg/kg) injection improved glucose intolerance, insulin resistance, oxidative stress and inflammation response in HFD mice. Ginsenosides Rc treatment accelerates peroxisome proliferator activated receptor alpha (PPAR-α, 19013)-mediated fatty acid oxidation in vivo and in vitro. Hepatic specific SIRT6 deletion abolished ginsenoside Rc-derived protective effects against HFD-induced NAFLD. Conclusion: Ginsenosides Rc protects mice against HFD-induced hepatosteatosis by improving PPAR-α-mediated fatty acid oxidation and antioxidant capacity in a SIRT6 dependent manner, and providing a promising strategy for NAFLD.

• Advances in concrete construction
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• 제13권3호
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• pp.215-221
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• 2022

Considering their similar mass densities, an attempt was made to optimize the mix design of micro-concrete that used barite sand as an aggregate by substituting marble powder (5%, 10%, 20%, 30%, 40%, 50%, 70%), clay brick powder (30%, 50%, 70%), and fly ash (30%, 50%, 70%) for the concrete (by mass) to form specimens for shaking table tests. The test results showed that for these three groups of materials, the substitutions had little effect on the density. The barite sand played a decisive role in the density, and the overall density of the specimens reached approximately 2.9 g/cm³. The compressive strength and elastic modulus decreased with an increase in the substitution rates for the three types of materials. Among them, the 28 day compressive strength values of the 40% and 50% marble powder groups were 11.73 MPa and 8.33 MPa, respectively, which were 58.7% and 70.7% lower than the control group, respectively. Their elastic modulus values were 1.33×10⁴ MPa and 1.42×10⁴ MPa, respectively, which were 39.1% and 35% lower than those of the control group, respectively. The 28 day compressive strength values of the 50% and 70% clay brick powder groups were 13.13 MPa and 5.8 MPa, respectively, which were 53.8% and 79.6% lower than the control group, respectively. Their elastic modulus values were 1.54×10⁴ MPa and 1.19×10⁴ MPa, respectively, which were 29.7% and 45.4% lower than those of the control group, respectively. The 28 day compressive strength values of the 50% and 70% fly ash groups were 13.5 MPa and 7.1 MPa, respectively, which were 52.5% and 75% lower than those of the control group, respectively. Their elastic modulus values were 1.36×10⁴ MPa and 0.95×10⁴ MPa, respectively, which were 37.9% and 56.6% lower than those of the control group, respectively. There was a linear relationship between the 28 day compressive strength and elastic modulus, with the correlation coefficient reaching a value higher than 0.88. The test results showed that the model materials met the high density, low compressive strength, and low elastic modulus requirements for shaking table tests, and the test data of the three groups of different alternative materials were compared and analyzed to provide references and assistance for relevant model testers.

• 대한치과교정학회지
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• 제53권3호
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• pp.150-162
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• 2023

Objective: To investigate craniofacial differences in individuals with hypodontia and explore the relationship between craniofacial features and the number of congenitally missing teeth. Methods: A cross-sectional study was conducted among 261 Chinese patients (males, 124; females, 137; age, 7-24 years), divided into four groups (without hypodontia: no teeth missing, mild: one or two missing teeth, moderate: three to five missing teeth, severe: six or more missing teeth) according to the number of congenitally missing teeth. Differences in cephalometric measurements among the groups were analyzed. Further, multivariate linear regression and smooth curve fitting were performed to evaluate the relationship between the number of congenitally missing teeth and the cephalometric measurements. Results: In patients with hypodontia, SNA, NA-AP, FH-NA, ANB, Wits, ANS-Me/N-Me, GoGn-SN, UL-EP, and LL-EP significantly decreased, while Pog-NB, AB-NP, N-ANS, and S-Go/N-Me significantly increased. In multivariate linear regression analysis, SNB, Pog-NB, and S-Go/N-Me were positively related to the number of congenitally missing teeth. In contrast, NA-AP, FH-NA, ANB, Wits, N-Me, ANS-Me, ANS-Me/N-Me, GoGn-SN, SGn-FH (Y-axis), UL-EP, and LL-EP were negatively related, with absolute values of regression coefficients ranging from 0.147 to 0.357. Further, NA-AP, Pog-NB, S-Go/N-Me, and GoGn-SN showed the same tendency in both sexes, whereas UL-EP and LL-EP were different. Conclusions: Compared with controls, patients with hypodontia tend toward a Class III skeletal relationship, reduced lower anterior face height, flatter mandibular plane, and more retrusive lips. The number of congenitally missing teeth had a greater effect on certain characteristics of craniofacial morphology in males than in females.

• 디지털융복합연구
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• 제20권3호
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• pp.63-68
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• 2022

5G 서비스 등장한 후 우리의 생활 방식을 크게 변화시켰다. 특히 스마트폰과 Long Term Evolution을 통한 네트워크 서비스의 확산은 사람과 사람 사이에 음성, 문자통신에 한정되었던 기존 이동통신 방식을 완전히 바꾸었으며, 사용자와 기업 활동의 모든 측면에서 혁신적 서비스들이 등장하게 되었다. 본 연구는 5G 서비스의 사용자 사회적 특성과 사용자가 5G 서비스의 사용의도의 관계를 검증하였다. 독립변수(사회적 실재감, 주관적 규범)들과 매개변수(지각된 유용성) 및 종속변수(사용의도) 간의 관계를 규명하고, 측정변수 간의 영향관계에서 지각된 유용성의 매개효과를 검증하였다. 본 연구에서는 5G 서비스의 사용자를 대상으로 설문조사를 진행하여 실증분석을 위한 데이터를 수집하였다. 연구결과, 5G 서비스의 사용자 사회적인 요인에서 사회적 실재감, 주관적 규범이 사용자들의 사용의도를 높일 수 있는 적합한 변수임을 확인하였으며, 지각된 유용성의 매개효과도 있는 것으로 검증되었다. 본 연구는 실증분석을 통해 5G 서비스의 지속 가능하고 안전한 발전을 위한 시사점을 제시하였고, 향후 관련 연구의 방향에 대해서도 제시하였다.

• Molecules and Cells
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• 제46권6호
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• pp.360-373
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• 2023

Papillary thyroid carcinoma (PTC) is the most common subtype of thyroid carcinoma. Despite a good prognosis, approximately a quarter of PTC patients are likely to relapse. Previous reports suggest an association between S-phase kinase-associated protein 2 (SKP2) and the prognosis of thyroid cancer. SKP1 is related to apoptosis of PTC cells; however, its role in PTC remains largely elusive. This study aimed to understand the expression and molecular mechanism of SKP2 in PTC. SKP2 expression was upregulated in PTC tissues and closely associated with clinical diagnosis. In vitro and in vivo knockdown of SKP2 expression in PTC cells suppressed cell growth and proliferation and induced apoptosis. SKP2 depletion promoted cell autophagy under glucose deprivation. SKP2 interacted with PH domain leucine-rich repeat protein phosphatase-1 (PHLPP1), triggering its degradation by ubiquitination. Furthermore, SKP2 activates the AKT-related pathways via PHLPP1, which leads to the cytoplasmic translocation of SKP2, indicating a reciprocal regulation between SKP2 and AKT. In conclusion, the upregulation of SKP2 leads to PTC proliferation and survival, and the regulatory network among SKP2, PHLPP1, and AKT provides novel insight into the molecular basis of SKP2 in tumor progression.

• 한국엔터테인먼트산업학회논문지
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• 제13권8호
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• pp.181-187
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• 2019

청춘 멜로영화, 장르는 한국 영화업계의 강점으로 해외 시장에서도 통했다. 곽재용, 한국의 유명한 영화감독, 시나리오작가는 화면의 아름다움, 감정의 섬세함, 상상력이 풍부한 '청춘 멜로 영화'를 잘 찍는다. 그의 영화는 독특한 매력과 특징이 있는데, 이 글은 영상 스타일, 인물 만들기, 그리고 주제 사상의 세 가지 측면에서 서술되어 있다. 우선 영상 스타일 차원에서 어떻게 다른 서사적 기법과 렌즈 언어를 구사해 영화의 분위기를 조성하고, 황홀한 동양미를 만들어냈는지 분석한다. 또 감독을 둘러싸고 영화 속 캐릭터를 다르게 그려내고 디테일을 부각시켜 스토리에 복선을 깔고 해석하는 데 주력했다. 다시 한번, 주제사상을 중심으로 곽재용의 멜로영화의 희비가 엇갈리는 깊은 의미를 분석하는 것은 오늘날 현대인의 내면적 지향과 추구를 반영하는 것이기 때문에 사람들은 깊이 생각하고 깊이 생각해야 한다. 경제 글로벌화 된 현대, 각국의 문화교류 협력 한국의 뛰어난 많은 영화들은 연구할 가치가 있으며, 곽재용 감독의 청춘 멜로 영화를 연구함으로써 그 영화가 시장에서 유행하는 원인을 발굴하고 작품에 반영된 심미를 분석한다. 성적 특징을 살려 청춘 멜로 영화를 만든 경험을 정리한다.

• 무역학회지
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• 제48권4호
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• pp.23-45
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• 2023

본 연구는 제도적 거리와 해외직접투자의 관계를 규명하기 위한 것으로 중국의 해외투자를 대상으로 수행한다. 중국과 투자 유치국 간의 제도적 거리는 세계은행에서 발표한 제도적 품질을 이용한다. 2008년부터 2019년까지 중국이 투자한 50개국의 패널 데이터를 수집하여 패널 GLS 방법론을 통해 해외직접투자에 영향을 미치는 요인을 제도적 관점에서 다음의 3가지를 검증한다. 첫째, 중국의 해외직접투자 전체 국가들을 대상으로 제도적 거리의 절대값이 중국의 OFDI에 미치는 영향이다. 두 번째는 중국과의 제도적 거리가 양(+)인 국가와 음(-)인 국가의 두 그룹으로 나눈 후 각 그룹에서의 제도적 거리와 OFDI의 관계이다. 마지막으로 제도적 거리와 중국의 OFDI 사이의 비선형 관계이다. 이를 위해 제도적 거리의 제곱항을 분석 모형에 추가한다. 분석 결과는 다음과 같다. 제도적 거리는 중국의 OFDI와 정(+)의 관계가 확인된다. 중국보다 상대적으로 제도 품질이 높은 진출국 그룹에서는 제도적 거리와 OFDI는 역 U자형 관계가 나타나지만, 낮은 진출국 그룹에서는 정(+)의 관계가 나타난다. 또한, 중국의 OFDI는 제도적 요인과 경제적 요인의 복합적인 영향을 받는 것으로 확인된다. 본 연구의 결과는 FDI 유치국뿐만 아니라 다국적 기업의 FDI 진출국 선택에 있어서 시사점을 제공할 것이다.

• Journal of Ginseng Research
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• 제47권6호
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• pp.743-754
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• 2023

Background: Myocardial fibrosis post-myocardial infarction (MI) can induce maladaptive cardiac remodeling as well as heart failure. Although 20(S)-ginsenoside Rg3 (Rg3) has been applied to cardiovascular diseases, its efficacy and specific molecular mechanism in myocardial fibrosis are largely unknown. Herein, we aimed to explore whether TGFBR1 signaling was involved in Rg3's anti-fibrotic effect post-MI. Methods: Left anterior descending (LAD) coronary artery ligation-induced MI mice and TGF-β1-stimulated primary cardiac fibroblasts (CFs) were adopted. Echocardiography, hematoxlin-eosin and Masson staining, Western-blot and immunohistochemistry, CCK8 and Edu were used to study the effects of Rg3 on myocardial fibrosis and TGFBR1 signaling. The combination mechanism of Rg3 and TGFBR1 was explored by surface plasmon resonance imaging (SPRi). Moreover, myocardial Tgfbr1-deficient mice and TGFBR1 adenovirus were adopted to confirm the pharmacological mechanism of Rg3. Results: In vivo experiments, Rg3 ameliorated myocardial fibrosis and hypertrophy and enhanced cardiac function. Rg3-TGFBR1 had the 1.78×10^-7 M equilibrium dissociation constant based on SPRi analysis, and Rg3 inhibited the activation of TGFBR1/Smads signaling dose-dependently. Cardiac-specific Tgfbr1 knockdown abolished Rg3's protection against myocardial fibrosis post-MI. In addition, Rg3 downregulated the TGF-β1-mediated CFs growth together with collagen production in vitro through TGFBR1 signaling. Moreover, TGFBR1 adenovirus partially blocked the inhibitory effect of Rg3. Conclusion: Rg3 improves myocardial fibrosis and cardiac function through suppressing CFs proliferation along with collagen deposition by inactivation of TGFBR1 pathway.

• Molecules and Cells
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• 제46권10호
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• pp.592-610
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• 2023

The Hippo kinase cascade functions as a central hub that relays input from the "outside world" of the cell and translates it into specific cellular responses by regulating the activity of Yes-associated protein 1 (YAP1). How Hippo translates input from the extracellular signals into specific intracellular responses remains unclear. Here, we show that transforming growth factor β (TGFβ)-activated TAK1 activates LATS1/2, which then phosphorylates YAP1. Phosphorylated YAP1 (p-YAP1) associates with RUNX3, but not with TEAD4, to form a TGFβ-stimulated restriction (R)-point-associated complex which activates target chromatin loci in the nucleus. Soon after, p-YAP1 is exported to the cytoplasm. Attenuation of TGFβ signaling results in re-localization of unphosphorylated YAP1 to the nucleus, where it forms a YAP1/TEAD4/SMAD3/AP1/p300 complex. The TGFβ-stimulated spatiotemporal dynamics of YAP1 are abrogated in many cancer cells. These results identify a new pathway that integrates TGFβ signals and the Hippo pathway (TGFβ→TAK1→LATS1/2→YAP1 cascade) with a novel dynamic nuclear role for p-YAP1.

• Korean Journal of Radiology
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• 제22권9호
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• pp.1525-1536
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• 2021

Objective: To investigate the feasibility of cine three-dimensional (3D) balanced steady-state free precession (b-SSFP) imaging combined with a non-local means (NLM) algorithm for image denoising in evaluating cardiac function in children with repaired tetralogy of Fallot (rTOF). Materials and Methods: Thirty-five patients with rTOF (mean age, 12 years; range, 7-18 years) were enrolled to undergo cardiac cine image acquisition, including two-dimensional (2D) b-SSFP, 3D b-SSFP, and 3D b-SSFP combined with NLM. End-diastolic volume (EDV), end-systolic volume (ESV), stroke volume (SV), and ejection fraction (EF) of the two ventricles were measured and indexed by body surface index. Acquisition time and image quality were recorded and compared among the three imaging sequences. Results: 3D b-SSFP with denoising vs. 2D b-SSFP had high correlation coefficients for EDV, ESV, SV, and EF of the left (0.959-0.991; p < 0.001) as well as right (0.755-0.965; p < 0.001) ventricular metrics. The image acquisition time ± standard deviation (SD) was 25.1 ± 2.4 seconds for 3D b-SSFP compared with 277.6 ± 0.7 seconds for 2D b-SSFP, indicating a significantly shorter time with the 3D than the 2D sequence (p < 0.001). Image quality score was better with 3D b-SSFP combined with denoising than with 3D b-SSFP (mean ± SD, 3.8 ± 0.6 vs. 3.5 ± 0.6; p = 0.005). Signal-to-noise ratios for blood and myocardium as well as contrast between blood and myocardium were higher for 3D b-SSFP combined with denoising than for 3D b-SSFP (p < 0.05 for all but septal myocardium). Conclusion: The 3D b-SSFP sequence can significantly reduce acquisition time compared to the 2D b-SSFP sequence for cine imaging in the evaluation of ventricular function in children with rTOF, and its quality can be further improved by combining it with an NLM denoising method.