• International Journal of Industrial Entomology and Biomaterials
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• v.34 no.1
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• pp.11-15
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• 2017

We constructed the fibroin H-chain expression system to produce enhanced cyan fluorescent proteins (ECFP) in transgenic silkworm cocoon. Fluorescent cocoon could be made by fusing ECFP cDNA to the heavy chain gene and injecting it into a silkworm. The ECFP fusion protein, each with N- and C-terminal sequences of the fibroin H-chain, was designed to be secreted into the lumen of the posterior silk glands. The expression of the ECFP/H-chain fusion gene was regulated by the fibroin H-chain promoter. The use of the 3xP3-driven EGFP cDNA as a marker allowed us to rapidly distinguish transgenic silkworms. The EGFP fluorescence became visible in the ocelli and in the central and peripheral nervous system on the seventh day of embryonic development. A mixture of the donor and helper vector was micro-injected into 1,020 Kumokjam, bivoltin silkworm eggs. We obtained 6 broods. The cocoon was displayed strong blue fluorescence, proving that the fusion protein was present in the cocoon. Accordingly, we suggest that the ECFP fluorescence silk will enable the production of novel biomaterial based on the transgenic silk.

• 한국가시화정보학회:학술대회논문집
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• 2004.12a
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• pp.1-13
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• 2004

A scanning stereo-PIV system was developed to measure the three-dimensional distribution of three-component velocity in a turbulent round jet. A laser light beam produced by a high repetition rate YLF pulse laser was expanded vertically by a cylindrical lens to form a laser light sheet. The light sheet is scanned in a direction normal to the sheet by a flat mirror mounted on an optical scanner, which is controlled by a programmable scanner controller. Two high-speed mega-pixel resolution C-MOS cameras captured the particle images illuminated by the light sheet, and stereoscopic PIV method was adopted to acquire the 3D-3C-velocity distribution of turbulent round jet in an octagonal tank filled with water. The jet Reynolds number was set at Re=1000 and the streamwise location of the measurement was fixed at approximately x = 40D. Time evolution of three-dimensional vortical structure, which is identified by vorticity, is visualized. It revealed that the existence of a group of hairpin-like vortex structures was quite evident around the rim of the shear layer of the jet. Turbulence statistics shows good agreement with the previous data, and divergence of a filtered (unfiltered) velocity vector field was $7\%\;(22\%)$ of root-me an-squared vorticity value.

• Proceedings of the Korea Institute of Convergence Signal Processing
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• 2001.06a
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• pp.165-168
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• 2001

본 논문에서는 카메라 또는 카메라 플랫폼의 흔들림 등 외부 영향과 비디오 시퀀스내의 모션이 함께 존재할 경우 출렁이는 비디오를 전자적으로 안정화하는 방법을 제안한다. 일반적인 영상 안정 시스템은 모션 측정과 모션 보상의 두 과정으로 구성되는데 모션 측정에서는 프레임간 모션 모델을 가정하고 파라메타를 측정하며 모션 보상에서는 측정된 파라메타를 이용하여 모션을 보상한다. 영상 내에 카메라 모션 이외의 움직임이 있을 경우 파라메타의 측정을 일관성 없게 만들 수 있으므로 이를 해결하기 위해 MVSD(Motion Vector Scatter Diagram)에 기반한 영상 안정 방법이 제안되었다. 그러나 이 방법은 최적화 파라메타를 정량화 하는데 한계가 있고 또한 계산 시간이 오래 걸리는 단점이 있어 이의 해결을 위해 본 논문에서는 선 기반(Line-based) 영상 안정 방법을 제안한다. 이 방법은 먼저 기준 영상에서 median filter를 이용해 영상 내의 코너를 검출하고 특징적인 두 점을 선택하여 이를 선으로 연결한다. 현재 영상에서 correlation을 이용하여 상응하는 두 특징점을 찾고 subpixel 방법으로 정확한 위치를 계산하여 선을 구한다. 이 두 선을 일치시키는 과정에서 모션 파라메타를 구하는데 먼저 평행 이동을 통해 한쪽 글을 일치시키고 이 과정에서 translation x, y 파라메타를 구한다. 다음 단계에서 한 쪽 끝이 일치된 두 선이 이루는 각을 계산하여 rotation 파라메타를 구한다. 이 방법으로 구해진 파라메타를 이용하여 모션 보상을 함으로서 영상 안정을 이를 수 있었다.

• Journal of Biomedical Engineering Research
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• v.19 no.3
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• pp.233-242
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• 1998

In anatomical aspects, magnetic resonance image offers more accurate information than other medical images such as X ray ultrasonic and CT images. This paper introduces a method that recognizes disk diseases from spine MR images. In this method, image enhancement, image segmentation and feature extraction for sagittal plane and axial plane images are performed to separate the disk region. And then template matching method is used to extract disease region for axial plane imges. Finally, disease feature vectors are integrated and disease discrimination processes are performed. Experimental results show that the proposed method discriminates between normal and diseased disk with a considerable recognition ratio.

• Journal of Microbiology and Biotechnology
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• v.15 no.3
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• pp.466-471
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• 2005

An Escherichia coli strain with high phytase activity was screened from pig excreta. The phytase gene, appA, was amplified by PCR technique. To obtain large amounts of appA phytase, the appA gene was subcloned into the baculovirus transfer vector pVL1393 under the control of the Polyhedrin promoter. The recombinant baculovirus harboring the appA gene was obtained after co-transfection and screening. The early $5^{th}$ instar larvae of silkworm were infected with the recombinant virus. Using this system, the appA phytase was overproduced up to 7,710 U per ml hemolymph. SDS-PAGE analysis revealed the baculovirus-derived appA phytase to be approximately 47 kDa in size. The optimal temperature and pH of the expressed phytase were $60^{\circ}C$ and pH 4.5, respectively. The enzymatic activity was increased by the presence of 1 mM $Ca^{2+}$, 1 mM $Mn^{2+}$, or $0.02\%$ Triton X-100.

• The Bulletin of The Korean Astronomical Society
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• v.36 no.2
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• pp.64-64
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• 2011

We present a new quasi-stellar object (QSO) selection algorithm using a Support Vector Machine, a supervised classification method, on a set of extracted time series features including period, amplitude, color, and autocorrelation value. We train a model that separates QSOs from variable stars, non-variable stars, and microlensing events using 58 known QSOs, 1629 variable stars, and 4288 non-variables in the MAssive Compact Halo Object (MACHO) database as a training set. To estimate the efficiency and the accuracy of the model, we perform a cross-validation test using the training set. The test shows that the model correctly identifies ~80% of known QSOs with a 25% false-positive rate. The majority of the false positives are Be stars. We applied the trained model to the MACHO Large Magellanic Cloud (LMC) data set, which consists of 40 million lightcurves, and found 1620 QSO candidates. During the selection, none of the 33,242 known MACHO variables were misclassified as QSO candidates. In order to estimate the true false-positive rate, we crossmatched the candidates with astronomical catalogs including the Spitzer Surveying the Agents of a Galaxy's Evolution (SAGE) LMC catalog and a few X-ray catalogs. The results further suggest that the majority of the candidates, more than 70%, are QSOs.

• YAKHAK HOEJI
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• v.47 no.3
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• pp.184-189
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• 2003

Peptide deformylase (PDF) is essential and unique to bacteria, thus making it an attractive target for the discovery of novel antibacterial drugs. PDF deformylates the N-formylmethionine of newly synthesized polypeptides in prokaryotes. In this study, a pdf gene from Staphylococcus aureus 6538p was cloned in pET-14b vector and PDF protein was over-produced in Escherichia coli BL21 (DE3). NH$_2$-terminal His-tagged PDF protein was purified by nickel-nitrilotriacetic acid (Ni-NTA) metal-affinity chromatography. Enzymatic activity of purified 6xHis-tagged PDF was tested on the substrate (formyl-Methionine-Alanine-Serine) by formate dehydrogenase-coupled spectrometric assay of peptide deformylase. For the discovery of new PDF inhibitors from chemical libraries and culture broths of soil bacteria, a target-oriented screening system using a 96-well plate was developed. About 3,000 commercial chemical libraries were tested in this screening system, and 2 chemicals (0.07％) among them showed an inhibitory activity against PDF enzyme. This result showed that a new screening system can be used for the discovery of new PDF inhibitors.

• Bulletin of the Korean Chemical Society
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• v.28 no.4
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• pp.574-580
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• 2007

Full-length chloride intracellular channel protein 1 (CLIC1) is a member of the family of proteins related to bovine chloride intracellular channel p64. Mutations in the SEDL gene cause spondyloepiphyseal dysplasia tarda (SEDT), a rare X-linked chondrodysplasia. The link between the intracellular chloride channels and SEDL is an important step toward understanding their functional interplay. In the present study, CLIC1 protein was subcloned into the pGEX-KG vector and overexpressed in XL-1 blue cells. We developed a large-scale expression system composed of glutathione S-transferase (GST) fused with a 240-amino-acid CLIC1 protein in Escherichia coli. The soluble CLIC1 protein was successfully purified to homogeneity, and its purity, identity, activity and conformation were determined using SDS-PAGE, MALDI-MS, biophotometer and circular dichroism spectroscopic studies. The binding of both CLIC1 and SEDL proteins in vitro was detected by BIAcore biosensor and fluorescence measurements.

• Proceedings of the IEEK Conference
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• summer
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• pp.23-26
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• 2004

Five-port reflectometer which consists of a ring with 5 arms (two inputs, three outputs) and three RF power detectors has been used as a vector network analyser, a demodulator in the homodyne receiver as well as in Phase Looked Loop (PLL) and so on. Calibration of five-port reflectometer is an important task. In this paper, we present a calibration method of five-port for a propagation channel sounder. The method is based on measurement of the phase differences between the three voltages at the five-port's outputs in order to determine the ratio of two input incident waves. The frequency channel sounder based on five-port is calibrated for each frequency from 2.2 GHz to 2.6 GHz with 1 MHz step. This method can also determine the absolute delays of each propagation path in the propagation channel. The calibration method is validated using measurement data.

• The Bulletin of The Korean Astronomical Society
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• v.41 no.1
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• pp.46.1-46.1
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• 2016

The formation process of a current sheet is important for solar flare from a viewpoint of a space weather prediction. We therefore derive the temporal development of the spatial and statistical distribution of electric current density distributed in a flare-producing active region to describe the formation of a current sheet. We derive time sequence distribution of electric current density by applying a nonlinear force-free approximation reconstruction to Active Region 12158 that produces an X1.6-class flare. The time sequence maps of photospheric vector magnetic field used for reconstruction are captured by a Helioseismic and Magnetic Imager (HMI) onboard Solar Dynamic Observatory (SDO) on 10th September, 2014. The spatial distribution of electric current density in NLFFF model well reproduce observed sigmoidal structure at the preflare phase, although a layer of high current density shrinks at the postflare phase. A double power-law profile of electric current density is found in statistical analysis. This may be expected to use an indicator of the occurrence of a solar flare.