• 한국환경과학회지
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• 제7권5호
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• pp.599-605
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• 1998

The purpose of this work was to examine whether X-Y chromosome dissociation in the primary spermatocytes of mice could be used as an in vivo short-term assaying system that detect environmental mutagens. Four alkylating agents(EMS, MMS, MMC and MNNG) which were known as strong mutagens were administered to BALB/c male mice 3-4 months old. In the control group, the mean frequencies of previously dissociated X and Y chromosomes and autosomes were 7.17% and 2.12%, respectively. Compared to the control group, mutagen-treated groups have no significant differences in dissociation rate of autosomes, while these poops were about 1.2-2.5 times higher in the frequencies of X-Y dissociation. Generally, X-Y dissociation frequency increased consistently with the concentration of mutagens whereas the tendency of autosome dissociation frequency was variable among several mutagens. These results suggest that X-Y dissociation in the primary spermatocytes of mice is applicable as an vivo short-term assaying system for environmental mutagens. There were significantly distinct increase in dissociation of X-Y chromosome in both the hybrid and parents but the X-Y previous dissociation of hybrid appeared higher frequency than BALB /c and wild mice. These results indicate that the factor related to binding X-Y chromosome is specific to strains.

• Toxicological Research
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• 제11권1호
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• pp.51-55
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• 1995

The present experiment was carried out to investigate whether X and Y chromosome dissociation in the primary spermatocytes of mice can be used as an in vivo assaying system that detect environmental mutagens. For this purpose, alkylating agents (EMS, MMS and MMC), which are strong mutagens, were administered to ICR male mice 12-15 weeks old. The mean frequencies of previously dissociated X-Y chromosomes and autosomes of the control group were 7.34-7.45% and 0.92-1.04%, respectively. The frequencies of X-Y dissociation in the mutagen-treated groups with 10.0 mM EMS and 5.0 mM MMS were about 3.3-4.6 times higher than that in the control group, but there were no significant differences in dissociation of autosomes in both the control and the mutagen-treated groups. These results suggest that X-Y dissociation in the primary spermatocytes of mice can be used as an in vivo short-term assaying system for environmental mutagens.

• 생명과학회지
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• 제8권1호
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• pp.60-66
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• 1998

G- 및 C-banding법에 의한 ICR 생쥐의 유사분열과 감수분열 시기의 염색체 특징에 관하여 조사하였다. 이를 위한 염색체 표본 작성은 Imai et al.의 공기건조법을 다소 변형한 방법에 의하였다. 정소를 이용한 염색체 분석은 유사분열뿐만 아니라 감수분열단계들을 모두 관찰할 수 있었으며, C-banding에 의하여 X 염색체를 포함한 모든 염색체의 돈원체 부위가 강하게 염색되었다. 정상의 제 1 감수분열 전기와 중기 사이의 세포들에서 2가 염색체로 된 19쌍의 상염색체과 1개의 말단결합으로 된X-Y 염색체를 관찰할 수 있었다. 대조군의 제 1 정모세포에서 조기분리된 성염색체를 가지는 세포의 빈도는 약 7.45%였으나,alkylating agents 처리군에서는 대조군에서보다 약 3-4배 이상 높에 나타났다. 그리고 감수분열 단계의 염색체 표본에 C-banding을 적용함으로서 X-Y 염색체 조기분리의 관찰이 매우 용이하였다.