• Title/Summary/Keyword: X STRs

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Analysis of the Short Tandem Repeat Loci for STRX1, HPRTB, ARA, DYS390, DYS392 and DYS393 in Koreans

  • Seol, Hye-Won;Zaw Tun;Katsuya Honda;Shogo Misawa;Park, Kyung-Sook
    • Animal cells and systems
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    • v.5 no.3
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    • pp.237-241
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    • 2001
  • Three STR loci (STRX1[AGAT]$_{n}$, HPRTB[AGAT]$_{n}$ and ARA[AGC]$_{n}$) on X chromosome and three other STR loci (DYS390[CTG(A)T]$_{n}$, DYS392[ATT]$_{n}$ and DYS39[GATA]$_{n}$) on Y chromosome were analyzed in 154 unrelated healthy Korean subjects. Four loci (STRX1, HPRTB, DYS390 and DYS393) were amplified by quadruplex polymerase chain reaction (PCR) using fluorescent labeled primers (FLP). ARA and DYS392 were amplified separately using single PCR, similarly by using FLP. They were then run in an automated DNA sequencer and were analyzed with Genescan software. We found 7 alleles (308-332 bp) in STRX1, 7 alleles (275-299 bp) in HPRTB, 16 alleles (252-315 bp) in ARA, 6 alleles (203-223 bp) in DYS390, 7 alleles (245-263bp) in DYS392 and 5 alleles (116-132 bp) in DYS393. The *13(34%), *13(5l%), *23 (l8%), *23(50%), *14(39%) and *13(40%) alleles were observed to be the highest frequencies of STRX1, HPRTB, ARA, DYS390, DYS392 and DYS393, respectively. The detection of STR loci on sex chromosomes by quadruplex PCR allows simple determination of sex and identification of an individual. individual.

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Latral Composition Modulation 기법으로 성장된 InP/GaP 초격자의 분광특성

  • Sin, Yong-Ho;Kim, Yong-Min;Song, Jin-Dong
    • Proceedings of the Korean Vacuum Society Conference
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    • 2013.02a
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    • pp.483-483
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    • 2013
  • Latral Composition Modulation (LCM)으로 성장한 InP/GaP 초격자(Superlattice)의 선편광된 광발광(Photoluminescence) 특성을 저온(5 K)에서 측정하였다. LCM 기법은 z-축 방향으로 InP와 GaP를 단층 초격자(monolayer supperlattice)로 성장하는 과정에서 strain에 의해 x-y 평면으로 초격자가 형성되는 특별한 경우이다. 이렇게 성장된 LCM 초격자의 경우 In-rich 영역과 Ga-rich 영역이 교차로 성장되는 구조를 가지며 가전자대역(valence band)에서 무거운 양공과 가벼운 양공의 band mixing 이 일어나게 되어 선평광된 발광특성을 가진다. 우리는 저온 발광실험에서 In-rich 영역과 Ga-rich 영역의 재결합에 의해 나타나는 두 개의 독립된 전이 피크를 측정하였다. 이 두 피크는 [110] 방향의 편광에서 발광 강도가 최대치를 가지며 [1-10] 방향에서 최소값을 가짐을 보였다. 이때 전이 에너지의 경우 [110] 방향에서 [1-10] 방향으로 편광이 바뀔 때 Ga-rich 영역의 전이의 경우 적색편이를 나타낸 반면 In-rich 영역의 경우 청색편이를 보이는 현상을 발견하였다. 이러한 상반된 편이 현상은 서로 다른 3족 물질의 영역에 따라 격자 상수가 바뀌며 tensile strss와 compressive stress에 따른 가전자 대역의 band mixing 변화에 기인하는 것으로 여겨진다.

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A Case of Single-step Mutations at Two Short Tandem Repeat loci (D13S317 and DXS10148) among Three Generations of a Korean Family

  • Byeong Ju Youn;Kyungmyung Lee;Cho Hee Kim
    • Biomedical Science Letters
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    • v.28 no.4
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    • pp.327-333
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    • 2022
  • The DNA profiling of short tandem repeat (STR) markers is a powerful tool for forensic identification and forensic paternity testing. However, STR loci are susceptible to mutation that cause mismatches between parents and children when paternity is tested. Herein, we examined paternity disputes with 23 autosomal STR loci using two commercial human identification kits and revealed successive mismatches at the D13S317 locus across three generations of a Korean family. Additionally, we investigated 12 X-chromosomal STRs and discovered an inconsistency at the DXS10148 locus between the father and daughter of the same Korean family. Furthermore, we confirmed STR genotypes at the D13S317 and DXS10148 loci of the family using sequencing analysis. Consequently, we identified a successive single-step mutation at the D13S317 locus and one single-step mutation at the DXS10148 locus in three generations of the Korean family. Therefore, this case study may be useful for interpreting and understanding forensic paternity tests.