• Plant Breeding and Biotechnology
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• v.6 no.4
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• pp.337-353
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• 2018

We used Illumina/HiSeq sequencing for analysis of gene expression profiling among four maize seed types (dent, CM3 and CM6; waxy, CM5 and CM19) at 10 DAP (days after pollination). A total of 88,993,000 (CM3), 103,817,340 (CM6), 103,139,640 (CM5), and 66,978,958 (CM19) sequence reads were generated with read lengths of about 0.9, 1.0, 1.0, and 0.7 billion bp, respectively. We obtained 69.1 (CM3), 71.0 (CM6), 71.2 (CM5), and 71.8% (CM19) high quality reads from the raw data and compared them with reference RNA sequences in a public DB (NCBI). It was revealed that mapped reads were 58%, 63%, 62%, and 62% of the EST reference in CM3, CM6, CM5 and CM19, respectively; and more than 51,000 genes were expressed based on RPKM criteria (over 0.25 value) in each CM3, CM6, CM5, and CM19 inbred line. In differentially expressed gene (DEG) analysis, we found that 3,527 genes were differentially expressed by at least two-fold with 1,709 upregulated in the two waxy inbred lines and 1,818 upregulated in the two dent inbred lines. We also detected genes for the sucrose and starch biosynthesis pathways based on BINs, and different expression patterns between waxy and dent inbred lines were shown for the gene set for starch synthesis, such as sh2, bt2, du1, wx1, and ae1. Although some genes were more expressed in dent lines, most genes for starch synthesis were much expressed in waxy lines. Especially, there was greater expression of the sus2 gene in both waxy lines compared with the dent lines.

• Proceedings of the Korea Water Resources Association Conference
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• 2016.05a
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• pp.593-593
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• 2016

최근 기후변화에 따른 국내 기상특성이 변화함에 따라 가뭄, 하천건천화, 홍수 등 물 관련 재해 발생 빈도 및 규모가 점점 커지고 있으며, 세계적으로 홍수 발생 빈도뿐만 아니라 태풍 및 가뭄 발생 빈도도 꾸준히 증가하고 있어 광범위한 관측과 정확한 예측 및 즉각적 대처능력 확보를 위한 수재해 관리가 필요한 실정이다. 아울러 급격한 도시화에 따라 내수범람이 빈번하게 발생하기 때문에 재난발생 시 그 피해가 극대화로 직결되고 있어 도시에서 발생할 수 있는 내수범람을 정확하게 예 경보하기 위한 고해상도 실시간 강수관측이 필요하다. 또한, 유역차원의 홍수범람이 빈번하게 발생하고 있으며, 홍수기 홍수통제소의 댐수문 관리에 어려움이 따라 기상관련 재난이 발생할 수 있어, 유역 차원의 정확한 홍수량 예측과 예 경보 시스템 구축을 위해서는 고해상도의 실시간 강수관측을 통한 강수예측 기술이 중요하다. 이를 위해 위성, 레이더, AWS 등 각종 광역 관측 장비로부터 표출되는 데이터를 통합하고, 이를 종합적으로 분석하여 수리수문인자 및 기상인자로 전환할 수 있는 시스템을 개발 할 경우 가뭄, 하천 건천화, 홍수 등을 실시간으로 감시하고 평가 예측 할 수 있는 정보 생산이 가능할 것으로 판단된다. 이에 본 연구에서는 수재해 정보 플랫폼 서비스를 위하여 전체적인 시스템을 개발하기에 앞서 가능성을 타진하고 검증할 필요가 있는 주요 구간을 시험하는 개발 기법인 프로토타입을 우선적으로 개발한다. 주요 항목으로는 (1) 지속 산출 격자 자료에 대한 OGC WxS 또는 LOD 서비스 자동 연계, (2) 격자 자료 ?춤형 제공(해상도, 좌표계 등), (3) 기초, 분석 정보 제공 시스템 등을 프로토타입 대상으로 설정하고 웹 기반 수재해 정보 플랫폼 인터페이스를 구현한다. 개발된 플랫폼은 수재해 예측정보의 정확도를 향상시키고 국지적 침수재해 평가 예측 및 지역 맞춤형 재해평가 체계를 실현함으로써 국가 물 관련 재해를 혁신할 수 있는 기술을 확보하는 소중한 토대가 될 것으로 사료된다.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.31 no.2
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• pp.226-230
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• 1986

Two near-isogenic lines with dark and pale green leaves, derived from the F$\_$7/ generation of wxl26 cross were compared on photosynthetic capacity, growth parameters, grain yield and yield-related characters. Dark green-leaved lines contained much greater content of chlorophyll a and b than pale green-leaved ones, but chlorophyll a to b ratio showed no difference between them. The photosynthetic nte per unit leaf area was higher in dark green-leaved lines than in pale green-leaved ones in the flag leaves at heading stage, but that per unit chlorophyll content showed reversed result. The crop growth rate from transplanting to heading was consistantly higher in the dark green-leaved lines, resulting from their greater net assimilation rate. Dark green-leaved lines produced greater number of panicles and spikelets per hill, out yielding pale green-leaved lines, but ripened grain ratio and 1000-grain weight showed no differences between those lines.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.50 no.5
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• pp.346-355
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• 2005

To investigate the application of biochemical markers' and small-sample methods using whole-wheat flours for screening in early generation in Korean wheat breeding system, 74 Korean wheats, including cultivars, local breeding lines and experimental lines, were analyzed. Seed storage protein and amylose contents of grains were evaluated. Biochemical makers, including granule bound starch synthase (GBSS), high molecular weigh glutenin subunits (HMW-GS) and friabilin were also evaluated by using one-dimensional sodium dodecyl sulfate-polyacryla-mide gel electrophoresis with a single kernel. The small­sample methods, including modified SDS-sedimentation test (MST), micro-alkaline water retention capacity (AWRC) and whole-wheat flour swelling volume (WSV) were also tested in this study. Protein content, MST and AWRC was $11.0 - 15.8\%$, 2.7 - 26.2 ml and $71.9 - 109.7\%$, respectively. Apparent and total amylose content and WSV was $20.6 - 25.0\%$, $26.1 - 32.4\%$ and 9.0 - 16.9 ml, respectively. There were highly significant correlations between MST and AWRC (r=0.592, P<0.001), but Korean wheats showed no significant difference in protein content, amylose content and small-sample methods. In the biochemical markers, Korean wheats contained all three GBSS encoded by Wx loci, except for Suwon 252. Korean wheats showed the high frequency ($58.1\%$) of 1Dx2.2 + 1Dy12 subunits of HMW-GS. Friabilin band was present in 46 lines ($62.2\%$) and absent in 28 lines ($37.8\%$). Friabilin-absence lines showed the higher MST (14.9 ml) and AWRC ($92.1\%$) value than friabilin-presence lines (8.5 ml and $82.4\%$, respectively).

• KOREAN JOURNAL OF CROP SCIENCE
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• v.67 no.4
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• pp.265-273
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• 2022

Waxy corn (Zea mays L.), which contains homozygous mutant alleles for the waxy1 (wx1) gene, is widely consumed as a snack food in Asia. This study evaluated sixteen agronomic characteristics of inbred Korean waxy corn lines to aid development of high-quality waxy corn cultivars. The plant materials studied were 177 inbred waxy corn lines developed by the National Institute of Crop Science, Rural Development Administration, Republic of Korea. For the tested lines, days to tasseling and silking averaged 77.69±2.22 days (with a range of 56-97 days), and 81.12±7.56 days (66-99 days), respectively. Plant length ranged from 88 to 237 cm (averaged 164.88±22.67 cm), ear length averaged 11.75±2.52 cm (5.0-18.5 cm), and ear width averaged 2.94±0.68 cm (1.4-4.5 cm). The number of rows on each ear of corn averaged 12.22±2.22 (7-32 rows) and the kernel number averaged 24.30±4.22 (9-37 kernels) per row. The crude protein content was 12.05±1.53% (8.90-21.80%) and total starch content was 69.27±5.74% (49.5-83.9%). Principal component analysis revealed that ear width, grain length, ear length, days to tasseling, days to silking, percentage of ear setting height, and total starch are features that allow distinction between the 177 waxy inbred corn lines. Hierarchical cluster analysis identified twelve waxy inbred lines that produce tall plants and have a short silking period. These lines may improve yield among quickly growing corn varieties.