• The Korean Journal of Ecology
• /
• v.21 no.4
• /
• pp.337-344
• /
• 1998

To select tolerant species among the Korean major woody plants for restoring disturbed ecosystems by air and soil pollution, we transplanted the seedlings of 56 species in control and polluted sites within Yeocheon industrial complex area, and compared their aboveground growth characteristics such as total branch length, total leaf weight, and maximum photozynthetic rate. Tolerant species growting better in polluted site than in control site was Quercus variabilis, Pinus thunbergii, Q. aliena, P. densiflora, Styrax japonica, Alnus firma, Celtis sinensis, Elaeagnus umbellata, Q. serrata, japonica, Sorbus alnifolia, and Q. acutissimia in local tree occuring within polluted area group (80%), Ailanthus altissima in street tree group (20%), Populus tomentiglandulosa and A. hirsuta var. sibirica in fast growing tree group (50%), Acer ginala and Abies holophylla in late successional tree group (20%), Betulla platyphylla var. japonica, Acer truncatum, A. palmatum, Syringa dilatata, and Rosa multifora in garden tree group (38%), and Q. rubura, and Robinia pseudoacacia in foreign restoring tree group (20%), respectively. The remaining plant species, 37 species (57% of total species), were classified into sensitive species to pollution. Those tolerant species can be utilized for restoration of the degraded ecosystem in this polluted area.

• Korean Journal of Plant Taxonomy
• /
• v.37 no.2
• /
• pp.87-108
• /
• 2007

The pollen morphology of 31 taxa, representing all woody species from 12 genera of Fabaceae in Korea, was examined by light microscope and scanning electron microscope. The pollen grains were monad and prolate spheroidal or rarely subprolate in equatorial view. The pollen grain aperture was trizonocolporate or rarely trizonooperculate, which does not occur in the other families of Fabales in Korea. The exine showed a great variety of sculpture pattern, i.e. psilate, microperforate, densely microperforate, perforate, polygonal perforate, macroperforate, macroperforate with granules in holes, polygonal macroperforate, verrucate with pitted perforate striae, verrucate-perforate, granulate, rugulate, reticulate or refined reticulate. The pollen morphological data supported the idea that the genera Echinosophora and Sophora were congeneric. Caragana (Galegeae) was quite close to the Sophora group (Maackia, Sophora and Echinosophora) of the Sophoreae in each measured pollen parameter and exine sculpture pattern. Eleven genera of Fabaceae were recognized based on differences in aperture type, pollen grain shape and exine pattern, and a generic pollen key of the woody Fabaceae in Korea was developed. A key to pollen types of Lespedeza was also suggested. The examined taxa of Coronilla and Wisteria were recognized by their exine patterns.

• Journal of Plant Biotechnology
• /
• v.41 no.1
• /
• pp.33-37
• /
• 2014

Lychnis wilfordii (Regel) Maxim is a rare and valued ornamental plant. Germination rate reached 46.6% when seeds were treated with $100mg{\cdot}l^{-1}$ GA (Gibberellic acid). The highest callus induction was observed in the leaf explants of the seedlings on MS medium containing specific concentrations of $0.5mg{\cdot}l^{-1}$ BA ($N^6$-benzyladenine) and $3.0mg{\cdot}l^{-1}$ NAA (a-naphthalene acetic acid). The adventitious shoot was formed in 97.3% of calli on 1/2 WPM (Woody Plant Medium) medium. Shoot elongation of in vitro propagated plantlets was no difference among various medium. The plantlets grew well after transferring to the pot. This in vitro propagation protocol should be useful for conservation of this endangered plant.

• The Plant Pathology Journal
• /
• v.20 no.2
• /
• pp.142-146
• /
• 2004

Woody plant tissues contain great amounts of phenolic compounds and polysaccharides. These substances inhibit the activation of reverse transcriptase and/or Taq polymerase in RT-PCR. The commonly used multiple-step protocols using several additives to diminish polyphenolic compounds during nucleic acid extraction are time consuming and laborious. In this study, sodium sulfite was evaluated as an additive for nucleic acid extraction from woody plants and the efficiency of RT-PCR assay of commercial nucleic acid extraction kits and small-scale dsRNA extraction was compared. Sodium sulfite was used as an inhibitor against polyphenolic oxidases and its effects were compared in RNA extraction by commercial extraction kit and small-scale double-stranded RNA (dsRNA) extraction method for RT-PCR. During nucleic acid extraction, addition of 0.5%-1.5%(w/v) of sodium sulfite to lysis buffer or STE buffer resulted in lighter browning by oxidation than extracts without sodium sulfite and improved the RT-PCR detection. When commercial RNA extraction kit was used, optimal concentrations of sodium sulfite were variable according to the tested plant. However, with dsRNA as RT-PCR template, sodium sulfite 1.5% in STE buffer improved the detection efficiency of Apple chlorotic leaf spot virus (ACLSV) and Apple stem grooving virus (ASGV) in fruit trees, and reduced the unspecific amplifications signi-ficantly. Furthermore, when viruses existed at low titers in host plant, small-scale dsRNA extractions were very reliable.

• Korean Journal of Agricultural Science
• /
• v.46 no.3
• /
• pp.529-538
• /
• 2019

This study examined the changes in plant phenology of 12 woody species in Daegu University Forest, Gyeongsan-si, Gyeongsanbuk-do in 2007 and 2018. The Hobo was installed at a height of 1.2 meters to measure the microclimate such as air temperature and relative humidity to identify its effects on the changes in plant phenology. The number of trees surveyed were 42 individuals that included 12 species in 9 families, all of which are deciduous broad-leaved trees. The trends in temperature change in 2018 were similar to that in 2007, except for the temperatures in the warmest and coldest month. Compared to 2007, the average temperature of the warmest month in 2018 was $2.5^{\circ}C$ higher, and the coldest month was $3.3^{\circ}C$ lower. The lowest temperature from February to April in 2018 had the highest correlation with the blooming dates of the trees (r = 0.692) in 2018, and the highest temperature showed the lowest correlation (r = 0.392). The blooming date by species was significantly different (p < 0.05). The blooming date of 8 species (75%), including Prunus padus, was earlier by 1 to 16 days, while 4 species, such as Prunus armeniaca var. ansu, was later by 1 to 7 days in 2018 than that in 2007. However, the flowering duration did not have a significant effect on the plant phenology. The results can be used as basic data for long-term monitoring of plant phenology in the future, and follow-up studies on other environmental factors and physiological factors are needed.

• Mycobiology
• /
• v.45 no.1
• /
• pp.15-19
• /
• 2017

This study proposed a rapid method to quantify the colonization rate of arbuscular mycorrhizal fungi (AMF) in plant roots. The method involved the use of an image analysis software (WinRHIZO Pro). The colonization rate is defined as the ratio of the fungal body to the plant root area in a micrograph. Three seedlings of Chengiopanax sciadophylloides, a woody species that accumulates radiocesium, were collected from a secondary forest in the Yamakiya district of Kawamata, Fukushima Prefecture during May-September 2014. The colonization of AMF structures was examined under a light microscope, and the percentage of colonization was determined using the WinRHIZO method. The superiority of the new method was verified by comparing with a modified grid-line intersect method. The colonization of AMF was confirmed in all the seedlings, and a significant coefficient of determination ($R^2=0.94$) was found with both the methods. The results suggested that the WinRHIZO method is reliable for estimating the colonization of AMF in C. sciadophylloides.

• Journal of the Korean Society of Environmental Restoration Technology
• /
• v.13 no.1
• /
• pp.112-120
• /
• 2010

This study was carried out to develop the environmental friendly restoration technique for various disturbed slopes using native plants seed chips. As the result of the separated seeding using the developed seed chips of native plant combination, average germination rate and survival rate of trees, shrubs, and herbs were higher than those of the direct seeding, as 79~70%, 83~73%, and 59~53% or so, respectively. Also, the germination rates and the survival rates of trees, shrubs, and herbs in the separated seeding by the native plants seed chips were 10~15 times higher than those of the direct seeding. Numbers of plants survived were at least 10 times of that of the simple seeding. The native plants seed chip technique is more efficient in soil slopes compared with stone slopes. The application of the native plants seed chip technique makes the restoration stabilized through the herbs dominant vegetation to the woody dominant 1 within 3 years, and the Landscapes also changed from herbs to variously combined herbs and woody. The technique using native plants seed chips that can be applicable to various disturbed slops is more efficient than conventional restoration techniques in the respect of economics and landscape.

• The Korean Journal of Ecology
• /
• v.20 no.3
• /
• pp.163-168
• /
• 1997

Taxa of the herbivorus insect larvae and their foodplant species were surveyed in a temperate forest of Namhansansung Area, Sungnam City, Kyonggi Province, in 1994-1996 growing season, Sixty two taxa of insect larvae fed on leaves of 18 woody species in 11 families during three growing season. Larvae began to be detected from the mid-April when the leafing time began. The number of larvae taxa reached to the maximum value(32 taxa) early in May, 1994. It was the time that the value of specific leaf area reached to the maximum. It decreased up to 3-4 taxa in the mid-June. Taxa of insect larvae were different year by year even in the same season. Most of larvae fed on various plant species, suggesting that they were generalist or polyphagous species. Fagaceae, Betulaceae, Ulmaceae, Rosaceae. Aceraceae, Ericaceae, Oleaceae and Styracaceae were fed on by many taxa of insect larvae, while Euonymus spp. (Celastraceae). Lindera obtusiloba(Lauraceae) and Symplocos chinensis for. pilosa(Symplocaceae) were mainly fed on by a few taxon.. Erannis, Calospilos and Phigalia were observed to feed on various species, but Illiberis, Pryeria and Chalocosia fed on only Rosaceae, Euonymus spp.(Cerastraceae) and Symplocos chinensis for. pilosa(Symplocaceae), respectively. An unidentified larva was observed only on Lindera obtusiloba (Lauraceae).

• Journal of the Korean Society of Environmental Restoration Technology
• /
• v.14 no.4
• /
• pp.55-66
• /
• 2011

The objective of this study was to investigate the possibility of seeding methods for quarry rehabilitation. To achieve the objective, the experiment was designed for rehabilitation of quarry with seed mixing types (woody type and herbaceous type) and forest topsoil. Seeds and seeding materials were applied to the quarry slope using hydroseeding measures. The study was conducted in limestone quarry (Lafarge Halla Cement Inc.) near Baekdu Daegan Mountain System at Okke, Kanwon-do. The experimental seedbed was set in 2007 and field investigation was carried out from 2007 to 2010. As the result of experiment, it was found that the early-phase pattern for surveyed species to establish was affected by the soil mixture types. The mixture type of herbaceous seeds resulted in the higher plant coverage than the woody seeds. The application of forest topsoil showed a potential increase the plant diversity, but it was affected by mixing seeds. Naturally-emerged species as Alnus hirsuta, Quercus mongolica will be useful for rehabilitation at the quarry and damaged slopes.

• Journal of Plant Biology
• /
• v.33 no.3
• /
• pp.173-182
• /
• 1990

To investigate the structure of the plant community structure of Mt. Yongmun in Kyonggi-do, fifty-four plots were set up by the clumped sampling method. The classification by TWINSPAN and DCA ordination were applied to the study area in order to classify them into several groups based on woody plant and environmental variables. By both techniques, the plant community were divided into two groups by the aspect. the dominant species of south aspect were Pinus densiflora, Quercus aliena, Q. mongolica, Carpinus laxiflora and of north aspect were Q. ongolica, Fraxinus rhynchophylla. The successional trends of tree species in south aspect seem to be from P. densiflora through Q. serrata, Q. aliena, A. mongolica to C. laxiflora. As a result of the analysis for the relationship between the stand scores of DCA and environmental variables, they had a tendency to increase significantly from the P. densiflora and Q. mongolica community to C. laxiflora and F. rhynchophylla community that was the soil moisture, the amount of soil humus and soil pH.