• Journal of Life Science
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• v.21 no.4
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• pp.584-588
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• 2011

This study was conducted to investigate the effects of organic materials, chitosan, wood vinegar, and EM active solution, on soil microbial and growth in Chinese cabbage. The organic materials were treated with chitosan, wood vinegar, and EM (effective microoganism) active solution, and the treatment concentration was 100 times solution and 1,000 times solution level with foliar application. The results are summarized as follows: Among foliar application of organic materials treatment plot[?], with 1,000 times solution level of chitosan was effective in inhibiting microbial growth in Chinese cabbage compared to other plots and control. The microorganism number in the soil for cultivation of Chinese cabbage increased with organic materials treatment plot as compared with control. Especially, 1,000 times solution level of chitosan showed the most significant effect.

• Journal of the Korean Wood Science and Technology
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• v.30 no.4
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• pp.80-86
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• 2002

Volatile compounds in three different kinds of crude vinegars obtained from oak (Quercus serrata), bamboo (phyllostachys) and pine (Pinus densiflora) species were analyzed by the solid-phase microextraction (SPME) method. A total of 264 peaks were detected on the chromatograms obtained from the polar (CBP 20) and the nonpolar (CBP 1) columns, which were used for analyzing the volatile compounds in these vinegars. The major volatile compounds identified by using the polar column were 2-butanone, acetic acid, guaiacol, phenol, cresols, 4-ethyl guaiacol, 4-ethyl phenol, and syringol. Using the nonpolar column, seven compounds could be identified: 1,2-dimethoxybenzyl alcohol, 1-hydroxy-2-butanone, 1-(2-furanyl)-1-propane, ethisolide, furfuryl acetate, 1,2-dimethoxybenzene, phenyl acetate. The volatile compounds were classified into five groups: phenols, neutral compounds, organic acids, esters and others. The phenols were the main component and comprised 49~65% of the volatile compounds of these vinegars. In the case of bamboo vinegar, the proportion of the phenols in the volatile compounds was lower than that of the two wood vinegars. However, the proportions of the neutral compounds and the organic acids were higher than those of the wood vinegars. Therefore, it seems that these differences of the proportions of the volatile compounds would make a certain difference of a smoke flavor between the bamboo vinegar and the wood vinegars.

• Journal of Korea Foresty Energy
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• v.20 no.1
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• pp.28-34
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• 2001

To analyze chemical compositions of softwood vinegar prepared with continuous carbonized kiln, the chemical compositions were analyzed by GC-MS spectrometry. The results were summarized as follow : 1. The amounts of methylalcohol and acetic acid and of vinegar were 0.12% and 0.8% respectively, and acidity was 0.85 2. Perfume components of vinegar were frufual, 5-meayl-2-furancarboxyaldehyde, 2,3-pentanedione, 2-butanol, 2,3-dihydrofuran, 1-(2-furanyl)-etanone, benzaldehyde, 2-furan carboxyaldehyde and acetic acid. 3 Vinegar prepared from softwood, so that murk amount of guaiacyl compound and phenol derivetives are produced from lignin and extractives was analγzed. 4 The yield of 4-methyl-di-tert-butylphenol was the highest in the nutural and carbonyl and acetic acid in the acid fractions, 3-ethylpentane in the basic fraction, and guaiacol in the phenolic fraction.

• Korean Journal of Organic Agriculture
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• v.14 no.4
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• pp.433-443
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• 2006

With treatment of Kitosan, Wood vinegear and EM(effective microoganism) which farmers call it as substance in fertilizing, conditioning and disease control substances, authors in vestigated on microorganisms change in soil and ealy growth characteristics of tomato. The results were summarized as follows: Among foliar application of kitosan, wood vinegear and EM(effective microoganism) treatments diluted by chitosan 500 times solution level was effective considering growth of tomato as compared other dilutions and control plot. Change of microorganism number in the soil for cultivation of tomato was increased with microorganism treatment plot as compared with control plot. Specially chitosan 500 times solution level showes the most significant effect.

• Journal of Animal Science and Technology
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• v.48 no.1
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• pp.59-68
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• 2006

This experiment was conducted to investigate the effects of dietary supplementation of activated charcoal (AC) mixed with wood vinegar on performance and egg quality in laying hens. A total of one hundred ninety two, 56-wk-old Lohmann brown laying hens were divided into four groups, consisting four replicates of 12 birds each and fed one of the four experimental diets containing AC 0%, AC 0.5%, AC 1%, AC 2% for 5 wks. The blood profiles and contents of total lipid and cholesterol, fatty acid profiles in egg yolk were determined. There were no significant differences in laying performance, feed intake and vitelline membrane strength among the treatments. The eggshell breaking strength and Haugh unit were significantly increased in 2% AC group compared to the other groups (P<0.05). Egg yolk index in 2% AC group was significantly higher (P<0.05) than those of the control and 0.5% AC group and yolk color was significantly improved by the dietary AC (P<0.05). The levels of serum total cholesterol, HDL-cholesterol and enzyme activities were not influenced by feeding AC. Total lipid of egg yolk in 1 % and 2% AC groups was significantly decreased (P<0.05) compared to that of the control, although there was no significant difference in yolk cholesterol. In conclusion, the addition of activated charcoal with wood vinegar in layer diet resulted in improving egg quality by means of enhancing eggshell breaking strength, Haugh unit, yolk index and yolk color.

• Korean Journal of Poultry Science
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• v.33 no.4
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• pp.283-293
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• 2006

Two experiments were conducted to investigate the effects of dietary activated charcoal mixed with wood vinegar (AC) on broiler performance and antibiotics residue in eggs. In experiment 1, a total of low hundred fifty, 1-day-old male broile. chicks (Ross) were divided into 6 groups, consisting 3 replicates of 25 birds each, and 134 one of the six experimental diets: devoid of AC and antibiotics (negative control), devoid of AC with 0.1% antibiotics (positive control), devoid of antibiotics with 1% AC, with 0.1% antibiotics and 1% AC, devoid of antibiotics with 2% AC, with 0.1% antibiotics and 2% AC, for 5 wks. Feed consumption and body weights were measured weekly. At the end of the experiment, eight birds from each group were selected and sacrificed. The relative weight of organs and characteristics of breast muscle were investigated. There were no significant differences in feed intake, body weight gain and feed conversion rate among the treatments. There were also no significant differences in the relative weight of breast meat, leg and liver. The abdominal fat tended to be decreased by the dietary AC. Significant differences were shown (P<0.05) in meat redness (a) and yellowness (b) among the treatments. However, the sensual characteristics of breast muscle was not affected by the dietary treatments. There were significant differences in total microbes, Coli forms and lactic acid bacteria (P<0.05) among the treatments. In experiment 2, a total of ninety, 40-wk-old Hy-line Brown laying hens were divided into 3 groups, consisting 3 replicates of 10 birds each and fed medicated control diet devoid of AC or diets containing 1% AC and 2% AC for 2 wks. The residue of antibiotics in Plasma and egg Yolk were significantly decreased (P<0.05) in 2% AC group compared to that of control. This study suggest that dietary AC may improve the external quality of edible meat and reduced antibiotics residue in chicken eggs without affecting laying performance.

• Korean Journal of Environmental Agriculture
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• v.25 no.3
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• pp.276-283
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• 2006

To evaluate the effectiveness of the foliar spray of calcium on "Baekdo" peach fruit, we carried out experiments in the orchards. The sprays were applied with $CaCl_2\;(Ca:\;400mg.kg^{-1})\;and\;CaCl_2$ with adjuvants (amino acid, $2g.kg^{-1}$; phytic acid, $2ml.kg^{-1}$ and wood vinegar, $2ml.kg^{-1}$) for four times from June 12 through July 4 at weekly intervals. The fruits and leaves were evaluated for Ca content, firmness and incidence of Brown rot caused by Monilinia fructicola at harvest To evaluate fruit quality included Ca content, firmness natural decay during the storage, the fruits were stored at room temperature for 14 days. The Ca content in leaf and fruit flesh at harvest was significantly increased (P=0.05) in $CaCl_2$ + amino acid treatment among $CaCl_2$ treatments. However, there was not significant Ca content in fruit peel. The firmness of flesh increased significantly (P=0.05) in $CaCl_2$ + amino acid treatment. The natural decay (Rhizopus stolonifers) during storage at the room temperature for 14 days, the fruit treated with $CaCl_2$ + wood vinegar exhibited lowest (P=0.05) incidence. Also, the firmness of the fruit during storage was firmer with treated $CaCl_2$ than untreated fruit. In the treatments of $CaCl_2$ + phytic acid and $CaCl_2$ + amino acid, it was possible to reduce incidence of Brown rot caused by M. fructicola most effectively in the field. In addition, inoculation with M. fructicola in fruits was also the most effective treatment for inhibiting disease development in vitro. These results suggested that the foliar spray of $CaCl_2$ with adjuvants increased the content of Ca and firmness of the fruits positively. It also inhibited the natural decay and the Brown rot effectively.

• Food Science of Animal Resources
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• v.27 no.1
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• pp.60-66
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• 2007

We investigated the effects of dietary supplements of probiotics, illite, active carbon and hardwood vinegar on growth performance, carcass characteristics and fatty acid composition in finishing pigs. One hundred fifty pigs ($Landrace{\times}Yorkshire{\times}Duroc$; $32.3{\pm}1.3 kg$ average initial body weight) were used in a 100 day experiment. Pigs were randomly placed into five experimental feeding groups (control, 0.2% probiotics, 1.0% illite, 1.0% active carbon and 1.0% hardwood vinegar) and were slaughtered at approximately 110 kg live weight. There was a slight, but insignificant increase in average daily body weight gain and feed intake among all treatment groups. Feed conversion was reduced when probiotics were added (p<0.05). There were no significant differences in the carcass rate and back fat thickness among the all treatment groups including controls. However, the frequency rate of grade A increased in the groups fed 1.0% active carbon and hard- wood vinegar compared to controls. Stearic acid content was lower in the groups fed probiotics illite active carbon and hardwood vinegar (p<0.05). Oleic acid contents were higher only in the groups fed 1.0% active carbon and hardwood vinegar (p<0.05).

• The Journal of Internal Korean Medicine
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• v.31 no.3
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• pp.650-666
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• 2010

Objectives : In this study, the author tried to investigate whether wood vinegar produced from Morus alba (MA) significantly affects the increase in airway epithelial mucosubstances and hyperplasia of tracheal goblet cells of rats, and in vitro airway mucin secretion and PMA- or EGF- or TNF-alpha-induced MUC5AC mucin production / gene expression from human airway epithelial cells. Materials and Methods : For the in vivo experiment, the author induced hypersecretion of airway mucus and goblet cell hyperplasia by exposure of rats to SO2 over 3 weeks. Effect of orally-administered MA over 2 weeks on increase in airway epithelial mucosubstances from tracheal goblet cells of rats and hyperplasia of goblet cells were assessed using histopathological analysis after staining the epithelial tissue with alcian blue. For the in vitro experiment, confluent RTSE cells were chased for 30 min in the presence of MA to assess the effect of MA on mucin secretion by enzyme-linked immunosorbent assay (ELISA). Also, effects of MA on PMA- or EGF- or TNF-alpha-induced MUC5AC mucin production and gene expression from human airway epithelial cells (NCI-H292) were investigated. Confluent NCI-H292 cells were pretreated for 30 min in the presence of MA and treated with PMA (10 ng/ml), EGF (25 ng/ml) or TNF-alpha (0.2 nm) for 24 hrs, to assess both effects of MA on PMA- or EGF- or TNF-alpha-induced MUC5AC mucin production by enzyme-linked immunosorbent assay (ELISA) and gene expression by reverse transcription-polymerase chain reaction (RT-PCR). Possible cytotoxicities of MA in vitro were assessed by examining LDH release from RTSE cells and the rate of survival and proliferation of NCI-H292 cells. In vivo liver and kidney toxicities of MA were evaluated by measuring serum GOT/GPT activities and serum BUN/creatinine concentrations of rats after administering MA orally. Results : 1. MA decreased the amount of intraepithelial mucosubstances of rats exposed to sulfur dioxide inhalationally. 2. MA decreased in vitro mucin secretion from cultured RTSE cells. 3. MA significantly inhibited PMA-, EGF-, and TNF-alpha-induced MUC5AC mucin productions and the expression levels of MUC5AC mRNA from NCI-H292 cells. 4. MA did not show either in vitro or in vivo hepatic or renal toxicities. Conclusion : The results from this study suggests that MA can regulate the secretion, production and gene expression of airway mucin observed in diverse respiratory diseases accompanied by mucus hypersecretion and does not show in vivo toxicity to liver and kidney functions after oral administration. Effects of MA should be further studied using animal experimental models that simulate the diverse pathophysiology of respiratory diseases via future research.

• Korean Journal of Soil Science and Fertilizer
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• v.45 no.5
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• pp.760-763
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• 2012

This study was conducted to investigate foliar treatment effects of organic agricultural materials for the environmentally-friendly cultivation of grape. The organic materials applied were chitosan, wood vinegar (pyroligneous acid), amino acid solution, and ginkgo leaf extract. All the organic materials were relatively strong acidic ranging lower than pH 4.6. when comparing with other organic materials, amino acid solution contained relatively high contents of selected plant nutrients, such as N, P, K, Ca, Mg, Cu, Fe, Mn, and Zn. As comparison of selected soil properties in the grape cultivating field, soil pH values were lower at the harvest stage than at the first stage of grape growing (before treating the organic materials), and electrical conductivity (EC) and soil organic matter content were higher at the harvest stage in the all plots. The concentrations of available phosphorus increased in most of the plot soils except in control plot (conventional treatment). The concentrations of exchangeable K decreased in the plot treated with ginkgo leaf extract and the control plot. The exchangeable Mg concentrations decreased in soils of all the plots. On the other hand, the concentrations of N and K in the grape leaves were higher with the treatments of chitosan and amino acid solution, P concentrations were higher with the applications of chitosan, wood vinegar and amino acid solution, and Ca and Mg concentrations were higher with chitosan and amino acid solution treatments, respectively, than with others. The yields of grape were higher, $1,581{\sim}1,583kg\;10a^{-1}$, in the control and wood vinegar treatment plots than others. Sugar contents of grape were not different among all the plots.