• Animal Bioscience
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• 제34권1호
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• pp.48-55
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• 2021

Objective: This study was conducted to investigate the effects of wilting and microbial inoculant treatment on the fermentation pattern and quality of Italian ryegrass silage. Methods: Italian ryegrass was harvested at heading stage and ensiled into vinyl bags (20 cm×30 cm) for 60d. Italian ryegrass was ensiled with 4 treatments (NWNA, no-wilting noadditive; NWA, no-wilting with additive; WNA, wilting no-additive; WA, wilting with additive) in 3 replications, wilting time was 5 hours and additives were treated with 106 cfu/g of Lactobacillus plantarum. The silages samples were collected at 1, 2, 3, 5, 10, 20, 30, 45, and 60 days after ensiling and analyzed for the ensiling quality and characteristics of fermentation patterns. Results: Wilting treatment resulted in lower crude protein and in vitro dry matter digestibility and there were no significant differences in acid detergent fiber (ADF), total digestible nutrient (TDN), water-soluble carbohydrate (WSC), ammonia content, and pH (p>0.05). However, wilting treatment resulted in higher ADF and neutral detergent fiber content of Italian ryegrass silage (p<0.05), and the WNA treatment showed the lowest TDN and in vitro dry matter digestibility. The pH of the silage was higher in the wilting group (WNA and WA) and lower in the additive treatment group. Meanwhile, the decrease in pH occurred sharply between the 3-5th day of storage. The ammonia nitrogen content was significantly lower in the additive treatment (p<0.05), and wilting had no effect. As fermentation progressed, the lactic and acetic acid contents were increased and showed the highest content at 30 days of storage. Conclusion: The wilting treatment did not significantly improve the silage fermentation, but the inoculant treatment improved the fermentation patterns and quality of the silage. So, inoculation before ensiling is recommended when preparing high quality of Italian ryegrass silage, and when wilting, it is recommended to combine inoculation for making high quality silage.

• Animal Bioscience
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• 제37권12호
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• pp.2091-2100
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• 2024

Objective: This trial was conducted to explore the impact of different wilting time of Italian ryegrass (IRG) in the field on the nutritional quality and fermentation characteristics of its silage. Methods: The harvested IRG was directly wilted in the field for 0 day (W0), 1 day (W1), 2 days (W2), and 3 days (W3), respectively, and tedded every 6 hours. And the preserved IRG was sampled at 1, 2, 3, 5, 10, 20, 30, and 45 days after ensiling and three replicates per treatment. Results: With the extension of wilting, the dry matter (DM) content and pH value of wilted IRG gradually increased (p<0.05). There was a downward trend in; neutral detergent fiber (NDF), acid detergent fiber (ADF), and hemicellulose with the increase of wilting time, but only W2 and W3 were significantly different from W0 (p<0.05). Crude protein (CP), in vitro DM digestibility), total digestible nutrients (TDN), and relative feed value decreased significantly with the increase of wilting time (p<0.05), except for W1. After 45 days of ensiling, W1 had the highest CP, TDN, and the lowest ADF and NDF. During ensiling, the increase of acetic acid and the decrease of WSC in W0 and W1 were similar, but the accumulation rate of lactic acid in W0 was faster than that in W1, resulting in the lowest pH value in W0. After 5 days of ensiling, the ratio of lactic acid to acetic acid in W1 stabilized at around 3:1, while W0 kept changing. Conclusion: Italian ryegrass that wilted in the field for 1 day effectively improved the dynamic changes in CP, TDN, ADF, and NDF and fermentation quality of silage. Therefore, in practice, W1 was more recommended in production of IRG silage.

• 한국초지조사료학회지
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• 제30권2호
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• pp.135-142
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• 2010

이 연구는 고품질 귀리 사일리지의 제조를 위한 수확시기별 적정 예건시간을 도출하고자 수행하였다. 시험은 국내 육성품종인 삼한귀리를 가을에 파종하고 이듬해 봄인 출수 후 20일 부터 10일 간격으로 3회에 걸쳐 수확하여 각 수확시기별로 0, 4, 8, 32시간동안 예건을 실시하였으며, 각 예건시간별 식물체의 수분변화와 사일리지 제조 후 품질을 분석하였다. 그 결과 귀리는 출수 후 20일 정도에 수확할 경우 4시간 정도 포장예건을 하고, 출수 후 30일 정도에 수확할 경우에는 사일리지 제조에 적합한 수분함량에 도달하여 예건이 필요하지 않은 것으로 나타났다. 사일리지도 수분함량이 약 55~65% 정도가 도달한 시기에 수확한 처리구나 그 수준에 도달하도록 예건한 처리구에서 전체적인 사료가치가 우수한 것으로 나타났다. 사일리지 품질 평가기준의 중요한 요인인 pH와 유기산은 수분변화에 따라 아주 큰 변이를 보였는데 55~65%의 수분조건에서 젖산 함량이 높게 나타났고, 초산과 낙산 함량이 낮았다. 따라서 귀리는 출수 후 20일 경에 수확 시에는 4시간 정도 포장예건을 하는 것이 바람직하고, 그 이후에는 바로 수확하여 곤포로 제조하여도 양질의 사일리지를 생산할 수 있을 것으로 판단된다.

• 한국초지조사료학회지
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• 제32권4호
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• pp.419-428
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• 2012

이 연구는 동계 사료맥류를 활용하여 건초대용 양질 헤일리지의 생산 가능성을 검토하기 위하여 맥종별 수확시기, 적정 건조시간 및 헤일리지 품질을 분석하였다. 그 결과 수확시기가 늦어질수록 건물수량과 TDN 수량은 높아지는 경향이었고, 단백질과 NDF, ADF 함량은 낮아지는 경향을 보였다(p<0.05). 헤일리지 조제를 위한 건조시간은 청보리, 귀리, 트리티케일, 총체밀 모두 유숙기에는 수분함량이 높음과 동시에 기상온도도 높지 않으므로 32시간 이상 예건이 필요하고, 황숙기에는 8~24시간 정도 예건이 필요하며, 완숙기에는 예건이 필요 없었다. 호밀은 출수 후 10일에는 32시간 이상, 출수 후 20일, 30일에서는 24시간 이상 예건이 필요하였다. 헤일리지 발효품질에서 pH는 모든 맥종에서 모두 수확시기가 늦어질수록 높아지는 경향을 보였고, 젖산함량은 유숙기에 가장 높은 값을 보였고, 수확시기가 늦어질수록 감소하였다(p<0.05). 초산도 수확시기가 늦어짐에 따라 감소하는 경향을 보였고, 낙산은 검출되지 않았다. 이상의 연구결과를 종합하여 볼 때, 사료맥류로 헤일리지 조제 시 단백질함량이나 발효품질 면에서는 수확이 빠를수록 유리하지만 우리나라 기상조건으로 볼 때 예건의 어려움이 있으므로, 호밀은 청예나 사일리지로 이용을 하고 청보리와 귀리, 트리티케일, 총체밀은 황숙기에 수확하여 8~24시간 정도 예건하면 헤일리지로 이용 가능성이 있는 것으로 판단되었다.

• 아시안잔디학회지
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• 제3권1호
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• pp.24-33
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• 1989

This study was conducted to investigate the effect of paclobutrazol [(2 RS , 3 RS )1-(4- chlor-ophenyl )-4, 4- dimethyl -2- (1, 2, 4- triazol -1- yl )- pentan -3-01] on the tolerance of hi-gh temperature and drought stress as related to growth retardation , iranspiration rate , soil water content , nitrogen level and photosynthetic rate in perennial ryegrass ( Loliurn perenne L . ' Omega H , ). Plants were given a 30 ml soil drench of paclohutrazol at the concentrations of 0, 0.01, 0.1, 1.0, 10.. 0, mg / 6 .5cm- diameter pot . The rcsults were as follows : 1. Increasing concentrations of paclohutrazul reduced plant height , leaf area , fresh weight and dry weight , hut increased chlorophyll content per unit area . The number of tillers and leaf width were not affected hy the paclobutrazol concentrations . 2. The proper concentration of paclohatrazol on growth retardation in perennial ryegrass was about I mq /pot , hut leaf deformity and severe growth retardation were shown at high concentration of 10 mq / pot . 3. Perennial ryegrasses grown at 30˚C were shown significantly short plant height and low leaf nitrogen level compared with those grown at 20˚C. Increasing concentrations of paclohutrazol at 20˚C increased nitrogen level hut it could not increase nitrogen level at 30˚C . 4. During the drought stress , increasing temperatures significantly promoted transpiration rate and wilting time . It took about 5 days at 20˚C and 3 days at 30˚C to reach wilting time of leaves from water stress treatment . Soil water contents at wilting time of non-treated controls were averaged 6. 871% at 20˚C and 6. 17% at 30˚C 5. Paclohutrazol reduced transpiration rate at high temperature and drought stress . Wilting appeared at the lower water content of soil according to increasing concentrations of paclobutrazol at 30˚C hut there were no differences among concentrations of at 20˚C. 6.Paclohutrazol treatment at 1 rag /pot reduced injury rate of leaves from 67.1 % and 100 % in control plants to 15.7% and 80% at 20˚C and 3010, respectively. 7. Photosynthetic rate per unit area was significantly reduced at high temperature . Paclohutrazol stimulated photosynthetic rate with increase of concentrations at 20˚C but there was no increasing effect at 30˚C.

• 한국초지조사료학회지
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• 제11권1호
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• pp.53-59
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• 1991

This experiment was conducted to investigate the effects of propionic acid and wilting on fermentation quality of Italian ryegrass silage. Grass material was wilted for a day before ensiling and propionic acid(0. 0, 0. 2. 0. 5 and 0.8 7~ of fresh matter) was applicated at ensiling time. Chemical composition, fermentation acids, pH, microbial population and distribution of the nitrogen contents were evaluated. The results obtained are summarized as follows: 1. The contents of dry matter and water soluble carbohydrate in the silage were increased by wilting, and crude fibre, NDF and ADF were decreased with increasing propionic acid levels. 2. The pH values of the silage increased by increasing DM content, but decreased with increasing propionic acid levels. Lactic acid content lowed in wilted silage, and acetic acid and butyric acid formation were decreased with increasing propionic acid levels. 3. Total nitrogen content in the silage was i n ~ r e a s e d ( ~ < . 05) by addition of propionic acid and wilting, and was the highest in the prewilting-0.8 Q propionic acid applicated silage. The production of NHBN was decreased with propionic acid and was the lowest in the 0.8 % treated silage. 4. The number of total bacteria and yeasts were estimated $10^6~10^7$, $10^2~10^3$ respectively. Moulds number were decreased with increasing propionic acid levels.

• 농업과학연구
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• 제37권2호
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• pp.171-175
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• 2010

This study was carried out to find the effect on growing media and proper time for water supply in rice seedling stages. Plant height of rice seedling for SIPAN-BRL among current several growing medias was higher in Chuchungbyeo than Ilpumbyeo, and that of root length was higher in check among used growing medias. Fresh and dry weight of shoot and root of two rice cultivar. Water time to absorption on each treatment of growing media concentration took similarly in SIPAN-BNH and check were similar. But 500 minutes did in 100ppm and did 370 and 470minute in 300 and 500ppm, respectively. Wilting time of rice seedling was appeared highly at 300ppm of SIPAN-BRL in Chuchungbyeo and 100ppm of SIPAN-BRL in Ilpumbyeo, while it prolonged at 300ppm in Chuchungbyeo and 500ppm in Ilpumbyeo, respectively. Water content at wilting stage was appeared highly at SIPAN-BRL 500ppm in both Chuchungbyo and Ilpumbyeo, while SIPAN-BNL was high in 300ppm of Chuchung-byeo and Ilpumbyeo, respectively. Plant and root length of SIPAN-BNL treated with 300ppm in 10day-rice seedling were highly appeared, while dry weight of shoot was high at 300ppm SIPAN-BNL.

• 한국토양비료학회지
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• 제22권1호
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• pp.53-60
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• 1989

수도(水稻)의 급성위조증상(急性萎凋症狀) 발생원인(發生原因)을 명확(明確)히 하기 위(爲)하여 전보(前報)에서 실시(實施)하였던 동일방법(同一方法)으로 우리나라의 기상조건(氣象條件) 및 답토양(畓土壤)을 공시토양(供試土壤)으로하여 인위적(人爲的)으로 급성위조증상(急性萎凋症狀)을 재현유기(再現誘起)하면서 근활력(根活力)과 도체내(稻體內)의 영양상태(營養狀態)가 위조증상발생(萎凋症狀發生)에 미치는 영향(影響)을 비교검토(比較檢討)한 결과(結果)를 요약(要約)하면 다음과 같다. 1. 공시(供試)한 품종(品種)의 위조증상발생(萎凋症狀發生) 및 피해정도(被害程度)와 발생시기(發生時期) 등(等)은 전보(前報)에서의 결과(結果)와 같았다. 2. 위조증상발생(萎凋症狀發生)과 관련(關連)하여 출수기경엽중(出穗期莖葉中)의 질소함량(窒素含量)과 칼리함량간(含量間)에는 "유신(維新)" - 0.9224, "밀양(密陽)23호(號)" - 0.8243의 높은 부(負)의 상관관계(相關關係)가 있었다. 3. 위조증상발생(萎凋症狀發生)과 생육시기별(生育時期別) 근활력(根活力)과의 관계(關係)는 위조증상(萎凋症狀)이 발생(發生)한 "유신(維新)" 및 "밀양(密陽)23호(號)" 모두 생식생장기(生殖生長期)보다 영양생장기(營養生長期)의 근활력(根活力)이 우세(優勢)하였으며 "유신(維新)"보다 "밀양(密陽)23호(號)"에서 근(根)의 활력(活力)이 높게 유지(維持)되었으나 이들 품종(品種)의 근활력(根活力)은 생식생장기(生殖生長期)에 현저(顯著)히 저하(低下)하는 특징(特徵)이 있는것이 재확인(再確認)되었다. 4. 위조증상발생(萎凋症狀發生)이 비교적(比較的) 적었던 칼리시용구(施用區)의 근활력(根活力)과 칼리무시용구(無施用區)의 근(根)의 활력간(活力間)에는 "유신(維新)" 0.8947, "밀양(密陽)23호(號)" 0.7301의 높은 정(正)의 상관계수(相關係數)를 나타내었다. 5. 위조증상발생(萎凋症狀發生)과 수분증산량(水分蒸散量)과의 관계(關係)를 보면 엽위별(葉位別) 증산량(蒸散量)은 출수전(出穗前)보다 출수후(出穗後)의 수분증산량(水分蒸散量)이 관계(關係)가 있는 것으로 추찰(推察)되었으며 위조증상(萎凋症狀)이 발생(發生)한 칼리무여용구(無旅用區)에서 수분증산량(水分蒸散量)이 많았고 상위엽(上位葉)보다 하위엽(下位葉), "밀양(密陽)23호(號)"보다 "유신(維新)"에서 명확(明確)하였다. 6. 등숙기(登熟期) "유신(維新)" 및 "밀양(密陽)23호(號)"의 간(稈)의 전분함량(澱粉含量)은 칼리시용량(施用量)에 따라 높은값을 나타냈으며 하위절간(下位節間)일수록 전분함량(澱粉含量)은 낮았으나 제(第)4절간(節間)에는 칼리무여용구(無旅用區)에 비(比)하여 칼리시용구(施用區)에서 크게 증가(增加)하는 것이 확인(確認)되었다.

• Animal Bioscience
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• 제34권1호
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• pp.56-65
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• 2021

Objective: This study was conducted to investigate the effects of molasses and Lactobacillus plantarum on the ensiling quality and in vitro rumen fermentation of sudangrass silage prepared with or without wilting. Methods: The ensiling experiment, measured with 3 replicates, was carried out according to a 2×4 (wilted stages×additives) factorial treatment structure. Dry matter of the fresh (210 g/kg fresh matter) or wilted (305 g/kg fresh matter) sudangrass were ensiled (packed into 5.0-L plastic jars) without additive (control) or with molasses (M), Lactobacillus plantarum (LP), or molasses + Lactobacillus plantarum (M+LP). After 60 days of ensiling, the silages were analyzed for the chemical, fermentation, and in vitro characteristics. Results: After 60 days of ensiling, the fermentation parameters were affected by wilted, the additives and the interactions of wilted with the additives (p<0.05). The M+LP treatment at wilted had higher lactic acid levels and V-score (p<0.05) but lower pH values and butyric acid concentrations than the other treatments. In comparison with sudangrass before ensiling, after ensiling had lower dry matter and higher non-fibrous carbohydrate. The in vitro gas production, in vitro dry matter digestibility, in vitro crude protein digestibility, and in vitro acid fiber detergent digestibility changed under the effects of the additives. Significant interactions were observed between wilted and the additives in terms of in vitro gas production at 48 h, asymptotic gas production, gas production rate, half time, and the average gas production rate. The total volatile fatty acid levels in the additive treatments were higher than those in the control. Conclusion: Wilting and supplementation with molasses and Lactobacillus plantarum had the ability to improve the ensiling quality and in vitro nutrient digestibility of sudangrass silage. The M+LP treatment at wilted exhibited the strongest positive effects on silage quality and in vitro ruminal fermentation characteristics.

• Journal of Animal Science and Technology
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• 제63권2호
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• pp.354-366
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• 2021

Limited data about the effects of various factors on forage quality and β-carotene content of rye produced in Korea are available, so this study investigated the effects of two preservation methods. Samples were collected from rye harvested every 5 days between April 25 and May 31, and comparisons were done among rye silage wilted for different periods of time and hay of three growth stages of rye. For the silage, dry matter (DM), acid detergent fiber (ADF), and neutral detergent fiber (NDF) contents increased with advanced maturity of rye, whereas crude protein, in vitro dry matter digestibility (IVDMD), total digestible nutrients (TDN), relative feed value (RFV), and DM loss decreased (p < 0.0001). Wilting increased the DM content and pH value significantly (p < 0.0001). Silage harvested at the heading stage had the lowest pH value (4.45), propionic acid (0.83 g/kg DM), butyric acid (0 g/kg DM), and fungi and yeast populations (3.70 Log CFU/g of fresh matter [FM]); conversely, it had the highest lactic acid (9.7 g/kg DM), lactic acid bacteria (LAB) (6.87 Log CFU/g of FM), total microorganisms (TM) (7.33 Log CFU/g of FM), and Flieg's score (70) (p < 0.0001). Wilting elevated LAB and TM populations, but it had no consistent effect on other fermentation products. Both delayed harvest and prolonged wilting decreased β-carotene content. Rye silage harvested around May 9 (heading stage) with 24 h of wilting was preferred for highland, Pyeongchang. For rye hay, advanced maturity decreased DM loss, IVDMD, TDN, and RFV, but it increased DM, ADF, and NDF significantly (p < 0.05). β-carotene was decreased by delay of hay-making. Consequently, to attain lower DM loss and higher hay quality, the harvest date of May 9 (heading stage) is recommended.