• Journal of Veterinary Science
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• 제22권6호
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• pp.81.1-81.9
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• 2021

Background: Suid gammaherpesvirus 3, 4, and 5 (porcine lymphotropic herpesvirus - PLHV-1, -2, and -3) are viruses that infect domestic and feral pigs. Objectives: This study examined the presence of PLHV DNA in biological samples from free-living wild boars circulating in a Brazilian geographical region with a high density of commercial domestic pigs. Methods: Lung samples of 50 free-living wild boars were collected by exotic wildlife controller agents between 2017 and 2019 in the state of Paraná, southern Brazil. Lung and spleen fragments were obtained from six fetuses collected by hysterectomy post mortem from a pregnant sow. A polymerase chain reaction (PCR) assay using consensus primers (pan-herpesviruses) was performed to detect PLHV DNA. The samples showing positive results for PLHV DNA were submitted to single-round PCR assays with the specific primers for identifying PLHV-1 (213-S/215-As), PLHV-2 (208-S/212-As), and PLHV-3 (886s/886As). The specificity of the species-specific PCR products was assessed by nucleotide sequencing of the amplicons. Results: Forty-eight (96%) of the 50 lung samples analyzed were positive for PLHV by PCR using pan-herpesvirus primers. In 33 (68.75%) of the positive samples, at least two PLHV species were identified simultaneously. The DNA of PLHV-1, -2, and -3 was found in free-living wild boars of all ages, but not in the fetuses, even though they were from a sow that tested positive for all three viruses. Conclusion: These viruses are endemic to the population of feral pigs in the Brazilian region evaluated, as well as in domesticated pigs.

• Journal of Animal Science and Technology
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• 제48권1호
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• pp.1-8
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• 2006

포유동물에서 KIT 유전자는 우성 백색의 모색발현에 관여하는 후보유전자로서 mast/stem cell growth factor receptor를 암호화하는 것으로 알려져 있다. 본 연구에서는 한반도에서 서식하고 있는 야생멧돼지의 모색발현에 있어서 KIT 유전자의 유전적 변이를 확인하기 위하여 PCR- RFLP와 염기서열 분석을 수행하여 유전적 변이를 관찰하였다. 멧돼지 KIT 유전자의 exon17- intron 17 경계부위에 대한 NlaⅢ-RFLP 결과 splicing mutation이 없고, exon 19 상에서의 SNP C2678T에 대한 AciⅠ-RFLP 결과 역시 백색 품종들의 유전자형과는 다르게 나타났다. 이상의 결과는 멧돼지 집단 내 교잡에 의해 백모색의 자손이 출현하지 않음을 의미한다. 또한 exon 19과 exon 20에서 새로운 SNP들이 확인되었으며 이들 중 exon 20에서의 SNP A2760G는 아미노산의 변화(iso-leucine→valine)를 초래할 수 있으나 모색 발현과의 연관은 확인할 수 없었다. 돼지 KIT 유전자의 exon 19, 20과 intron 19 상에서의 새로 발견된 SNP와 splicing mutation의 존재 여부 등은 품종특이적인 양상으로 확인되었고, 이 같은 결과는 돼지에서 백색 모색 발현을 설명할 수 있는 좋은 자료가 될 것으로 사료된다.

• 한국동물위생학회지
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• 제39권4호
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• pp.267-270
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• 2016

We determined the nationwide seroprevalence of hepatitis E virus (HEV) infection in the wild boar population in Korea. Enzyme-linked immunosorbent assay (ELISA) results showed that 42% of the 528 wild boars that were hunted between 2013 and 2014 were anti-HEV antibody positive. Furthermore, all Korean provinces showed an HEV seroprevalence between 9.8% and 51.1%, suggesting that wild boar HEV infection occurs throughout the country. Importantly, infected wild boar could act as a potential reservoir for HEV and could aid transmission to other animals and humans.

• 환경영향평가
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• 제29권3호
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• pp.230-238
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• 2020

본 연구는 비침습 샘플인 털을 이용하여 영월 한반도습지 내 서식하는 멧돼지(Sus scrofa)의 유전분석을 통하여 그들의 서식 생태를 유추하였다. 털 시료는 2018년 11월부터 2019년 5월까지 한반도습지(2.772㎢) 내에서 비빔목 및 헤어트랩을 이용하여 수집하였다. 털 시료로부터 DNA를 추출하여 개체의 종과 성을 PCR을 통해 파악하였으며 6개의 마이크로새틀라이트 마커를 이용하여 개체 구분과 개체 간 유전적 근연관계를 유추하였다. 수집된 털 시료 중 총 16개의 털이 멧돼지의 시료였으며, 이는 암컷 7마리, 수컷 3마리의 10개체로부터 수집된 것임이 판명되었다. 이 10개체의 유전적 관계를 추정해 본 결과, 이들이 만들어내는 45쌍 중에 9쌍의 개체가 친족관계일 가능성이 높게 나타났다. 개체 쌍의 친족관계와 털 시료가 채집된 위치를 함께 고려하여 본 결과, 한반도습지 일대에서 서식하는 멧돼지는 암컷과 그 자손으로써 모계 가족단위로 생활하는 것으로 추정되며, 이는 기존에 알려진 멧돼지의 습성과도 일치하는 결과이다. 그러나 본 연구에 사용된 샘플과 마이크로새틀라이트 마커의 수가 제한적이므로 향후 추가적인 분석이 필요하다.

• 대한전자공학회:학술대회논문집
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• 대한전자공학회 2006년도 하계종합학술대회
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• pp.719-720
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• 2006

Ad hoc networks does not need any wired network infrastructure. Therefore, they have been developed in temporary networks or mainly in military networks. Infostations offer geographically intermittent coverage at high speeds. Up-to-date there have been frequent big forest fires in Korea mountain areas. It is very important to detect them early to prevent them from being big disasters. In this paper, we propose a disaster emergency management system using sensor attached wild boars' mobility combined with infostation system. We also make a numerical analysis of the performance of the system.

• 한국동물위생학회지
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• 제46권1호
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• pp.59-66
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• 2023

Wild boar is closely related to domestic pigs in terms of genetic homogeneity and the possibility of a source of infection by contact. This study investigated the prevalence of viral diseases from wild boars inhabiting Gyeongsangnam-do, South Korea. A total of 374 blood samples were collected and subjected to antigen tests to detect African swine fever virus (ASFV), Porcine circovirus type-2 (PCV2), Porcine reproductive and respiratory syndrome virus (PRRSV). For seroprevalence, PCV2, PRRS, classical swine fever virus (CSFV), Aujezsky's disease (ADV), and foot and mouth disease virus (FMDV) were investigated. The antigenic analysis revealed 73 positive cases (19.5%) for PCV2, while no positive cases for ASFV and PRRSV. For the antibody test, 225 (60.2%), 2 (0.5%), and 48 (12.8%) cases were detected against PCV2, PRRSV, and CSFV, respectively. There were no antibodies detected against both ADV and FMDV. Our results suggest that the viruses infecting both wild boar and domestic pig, mainly PCV2, are circulating in the wild boar population thus, the consistent monitoring of prevalence in wild boar will be needed for transboundary spillover to the domestic pig.

• Parasites, Hosts and Diseases
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• 제51권3호
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• pp.379-381
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• 2013

To know the status of sparganum (plerocercoid of Spirometra erinacei) infection in the Korean wild life, several species of wild animals were captured in Gangwon-do and examined for their status of infection with spargana. From February to December 2011, a total of 62 wild boars, 5 badgers, 1 weasel, 1 Siberian chipmunk, and 53 wild rodents were captured, and their whole muscles were examined with naked eyes for the presence of spargana worms. From the weasel and 1 wild boar, a total of 5 spargana specimens were extracted. The weasel was for the first time recorded as an intermediate or paratenic/transport host of S. erinacei in Korea, and both the weasel (Mustela sibirica manchurica) and wild boar (Sus scrofa) were added to the list of wild animals carrying spargana.

• Asian-Australasian Journal of Animal Sciences
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• 제20권4호
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• pp.461-465
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• 2007

Porcine Endogenous Retroviruses (PERVs) are a major concern in relation to xenotransplantation. Previous research indicated that PERVs are present at about 50 copies in the pig genome and their chromosomal insertion sites are different among pig breeds. We examined nine Korean native pigs and seven Asian Wild Boars for the presence of a PERV-A at SSC 1q2.4 and a PERV-B at SSC 7p1.1-2 previously reported in a Large White pig. The PERV-B at locus 7p1.1-2 displayed insertional variability in Korean native pigs and Asian Wild Boars. Using the primers for the PERV-A at 1q2.4 from Large White pig, we only can amplify an unclassified 798 bp sequence, which showed insertional variability only in Korean native pigs. This study indicates that there are differences within and between Asian and European pigs in PERV insertions and suggests that selection could generate PERV-free lines of pigs more suitable for xenotransplantation.

• Journal of Forest and Environmental Science
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• 제38권1호
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• pp.55-63
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• 2022

The African swine fever virus (ASFV) remains contagious for a long time, not only in the carcass, but also in the bone marrow of an infected animal. The scavenging activity of various animals on ASFV-infected carcasses is a likely risk factor for ASFV transmission. Thus, we conducted this study to determine whether scavengers are potential vectors for ASFV. In nonprotected wild boar carcasses on the forest floor, we investigated the seasonal patterns of carcass decomposition and scavenger visits for feeding on them. The duration from fresh to early skeletonization (only bones and leather remaining) of adult carcasses was 37.6±23.1 days (n=3, range=11-51 days) in winter. The duration from fresh to later skeletonization (only bones and some fur remaining) of all carcasses, including subadult carcasses, was 8.3±2.5 days (n=4, range=7-12 days) in summer. At all three study sites, leopard cats (30.3%), large-billed crows (21.6%), and golden eagles (18.1%) were the frequently visiting species, representing more than 10% of the total visits (343 visits) in winter, whereas raccoons (21.9%), grey-backed thrushes (39.4%), and eyebrowed thrushes (14.7%) were the most frequent visitors in summer. In winter, crows or cinereous vultures were the first animals to arrive at a carcass; in summer, raccoons or crows arrived first. Our results showed that wild boars, raccoons, and leopard cats relatively frequently visited wild boar carcasses and stayed there for a long time. Wild rodents chewing on or staying near carcasses were photographed during winter. In addition to wild boars, thus, mammals, such as raccoons, leopard cats and rodents, and birds, such as accipitrids and thrushes, may be spreaders of ASFV in South Korea.

• Journal of Veterinary Science
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• 제22권2호
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• pp.28.1-28.6
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• 2021

An African swine fever (ASF) outbreak in wild boars was first reported on October 2, 2019, in South Korea. Since then, additional cases were reported in South Korea's border areas. We here report the identification of ASF virus (ASFV) DNAs from two out of eight environmental abiotic matter samples collected from areas where ASF-positive wild boar carcasses were found. Comparative genomic investigations suggested that the contaminating ASFV DNAs originated from the wild boar whose carcass had been found near the positive sample sites. This is the first report on the identification of ASF viral material in wild boar habitats.