• Environmental Analysis Health and Toxicology
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• v.4 no.1_2
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• pp.1-10
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• 1989

Experiments were performed on mice to investigate the influences of doxycycine and ethanol on the immune responses. Doxycycline was injected intraperitoneally and ethanol was administered in the drinking water. Mice were sensitized and challenged with sheep red blood cells. Immune responses were evaluated by humoral immunity, cellular immunity, peripheral circulating white blood cell and phagocyte activity. Pathotoxicological influences were measured by serum protein and albumin. The weight of spleen, thymus and liver were measured. Doxycline and ethanol combined administration decreased the weight of thymus and spleen. Humoral and cellular immune response were reduced by doxycycline administration. Especially ethanol combined administration significantly reduced humoral and cellular immune response. Phagocyte activity was increased by ethanol combined administration and peripheral circulating white blood cell was significantly increased by ethanol adminstration. Ethanol combined administration decreased serum A/G ratio.

• Journal of Veterinary Clinics
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• v.15 no.2
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• pp.352-357
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• 1998

Effects of chicken egg white derivatives (EWD and EF-203) on the changes of blood cells and the neutrophil phagocytic activity were examined in the rats. Rats were administered orally with either EWD (200 mg/kg) or EF-203 (200 mg/kg) for 3 days. Thereafter, the changes of blood cell values (RBC, WBC, platelets, PCV, differential count of neutrophils) and the phagocytic activity of neutrophils were evaluated for 7 days. The numbers of WBC and the differential count of neutrophils of rats administered with either EWD or EF-203 were significantly increased (p

• Biomedical Science Letters
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• v.30 no.1
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• pp.10-16
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• 2024

Oxidative stress caused by elevated reactive oxygen species (ROS) in the heart causes various heart diseases. Oxidative stress is known as a factor that causes diseases in various organs as well as the heart. Diseases such as heart failure, myocardial infarction, and cardiomyopathy caused by oxidative stress in the heart can be treated with medication or surgery. Recently, blood cells concentrate (BCC) is used in various treatment areas such as orthopedics, gynecology, and urology. BCC therapy is applied to treatment by concentrating platelets and white blood cells necessary for regeneration through simple centrifugation using autologous blood. As the platelets are activated, many growth factors are released from alpha granules of the platelets. Growth factors such as TGF-β1, PDGF, VEGF, and EGF derived from platelets are involved in various cell signaling pathway. Due to these growth factors, BCC can contribute to tissue regeneration and can treat various diseases. CD34+ cells contained in BCC may also play an important role in tissue regeneration. In this study, we investigated whether BCC has a regenerative effect on heart disease, and if so, what mechanism causes the effect. To observe this, cardiomyocyte cells were treated with H₂O₂ to induce oxidative stress. And the effect was confirmed in the presence or absence of BCC. As a result, in the presence of BCC, the oxidative stress of cardiomyocyte cells was reduced and cell damage was also reduced. These results suggest that BCC therapy can be a new treatment alternative for heart disease.

• Journal of The Korean Society of Grassland and Forage Science
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• v.35 no.3
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• pp.217-224
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• 2015

This study aimed to investigate the effects of Dandelion (Taraxzcum coreanum) supplementation on milk yield, milk composition and blood characteristics in lactating dairy cows. Eight lactating dairy cows were divided into two groups (control: TMR supplementation, treatment: TMR with Dandelion supplementation). The milk yield, milk fat, lactose, solids not fat (SNF) and somatic cells counts (SCC) were not significantly different between the control group and the treatment group, whereas milk protein, milk urea nitrogen (MUN) and free fat acid (FFA) were significantly higher in the treatment group compared to the control (p<0.05). The blood components of the treatment group were compared with those of the control group and only aspartate aminotransferase (AST) appeared significantly high (p<0.05). The other blood components were not significantly different in the two groups. Blood corpuscle components in the groups were not significantly different. Especially, all blood corpuscle components in the treatment group were within the normal range. However, the white blood cells (WBC), lymphocytes (LYM) and hematocrits (HCT) in the control group exceeded the normal range. Based on the above results, the addition of Dandelion to feed increased milk protein, MUN and FFA, but did not significantly affect the composition of the blood and corpuscle in Holstein milking cows.

• Journal of the Korean Society of Food Science and Nutrition
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• v.27 no.6
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• pp.1267-1272
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• 1998

In order to investigate the preventive and therapeutic of royal jelly on diabetes, the levels of blood glucose and serum lipids as well as the number of blood cells were determined in streptozotocin(STZ) diabetic rats. Rats were divided into seven groups. The RJ group was administered royal jelly and the STZ group was treated with streptozotocin to induce diabetes. To determine the preventive effect, diabetes was induced after administration of royal jelly for 2 weeks in group RS1/RS2. In group SR1/SR2 diabetic rats were administered royal jelly for 2 weeks to investigate the therapeutic effect. After 3 weeks, the body weight was reduced in STZ and SR1 groups and food intake was increased in the STZ, RS1 and SR1 groups. The blood glucose level was similar to the control group in the RJ, RS1 and RS2 groups and there was no effect in the other groups. The total lipid and triglyceride level were lower in the SR1 group as compared to STZ, and the total cholesterol level was higher in the STZ group. The index of atherogenesis was lower in the RJ and SR1 groups compared to the normal group. The number of red blood cells and hemoglobin was higher in the RJ and SR1 groups and the number of white blood cells was higher in the RJ and SR2 groups.

• Korean Journal of Agricultural Science
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• v.42 no.3
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• pp.201-205
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• 2015

The experiment was conducted to evaluate the effects of pathogenic Escherichia coli on diarrhea, growth performance, and blood profile of weaned pigs. A total of 48 pigs were used and housed in individual pens of disease containment chambers for 16 d (4 d before and 12 d after the first challenge [d0]). The treatments were with or without the pathogenic E. coli challenge (F-18 E. coli strain; heat-labile, heat-stable, and Shiga-like toxins). Pigs were orally inoculated with a dose of $10^{10}cfu$ E. coli per 3 mL PBS daily for 3 days. The common nursery diet and water were available at all times. The ADG, ADFI, G:F, diarrhea score, ratio of fecal ${\beta}$-hemolytic coliforms from total coliforms (RHT), and blood profile were measured. The pathogenic E. coli reduced (P < 0.05) ADG from d0 to 6 (117 vs. 297 g/d) and from d0 to 12 (377 vs. 238 g/d) compared with the control. Meanwhile, the pathogenic E. coli increased (P < 0.05) diarrhea score (average 3.4 vs. 1.4) and RHT (average 82 vs. 11%) on d3, 6, and 9 and the number of white blood cells (17.59 vs. $13.48{\times}10^3/{\mu}L$) on d6 compared with the control. No differences were found on ADFI and others in the blood profile (total protein and hematocrit). In conclusion, pathogenic E. coli used in this experiment successfully caused mild diarrhea, increased number of white blood cells, and adversely affected growth rate of weaned pigs.

• Natural Product Sciences
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• v.3 no.2
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• pp.89-99
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• 1997

Aminolysis, hydrazinolysis and alkylation of 4-methoxy and 4,9-dimethoxy-6-cyano-7-thione-5-methyl-7H furo [3,2-g] [1] benzopyridine (1 a-b) yielded 7N-substituted furobenzopyridine derivatives (2 a-e or the possible isomers 3 a-e and 4 a-b), (5 a,b and 6 a,b) and the ester (8 a,b). Hydrolysis of (la) with acetic acid gave the corresponding pyridone derivatives (7). Furobenzopyridinyl-7-thioacetyl hydrazide (9 a,b) have been prepared via alkylation of furobenzopyridine thione (1 a-b) with ethyl chloroacetate followed by condensation with hydrazine hydrate. Schiff base (11) was prepared by reacting (9a) with p. N,N-dimethyl aminobenzaldehyde in boiling ethanol. Treatment of (8a) with anthranilic acid gave the corresponding 7-substituted-4H-3,1-benzoxazine-4-one (10). We found that compound (11) increased bleeding, coagulating time, the total count of white blood cells, blood glucose level (cause hyperglycemia), enzymes (GOT, GPT) activities, concentration of urea and creatinine. On the other hand it decreased red blood cells number, haemoglobin content and haematocrite value.

• Journal of Pharmacopuncture
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• v.25 no.3
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• pp.250-257
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• 2022

Objectives: Bee venom (BV) therapy is performed by a bee sting or subcutaneous injection of BV. However, there is not much information on the effect of BV on blood parameters after entering the body. This project aimed to assess the side effects of subcutaneous BV injections in healthy rats by measuring the hematological and biochemical parameters. Methods: Various amounts of BV, including 100, 200, and 500 (㎍/day), were subcutaneously injected into rats for 30 days. The results showed that BV affected the metabolism of the liver, kidney, and glands. Results: An increase in blood sugar and a decrease in other biochemical parameters, including cholesterol, triglyceride, urea, creatinine AST, ALT, ALP, and phosphorous, were observed. Results also showed increased counts of white blood cells, neutrophils (%), and platelets and decreased levels of red cells, hemoglobin, and hematocrit. Conclusion: This study demonstrates that BV therapy in medical clinics requires routine care and testing to prevent eventual metabolic and anemia side effects.

• The Journal of the Korean dental association
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• v.10 no.10
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• pp.693-695
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• 1972

The authors had observed the clinical blood findings of dental patients. Sampling of studies was 187 persons coming in Infirmary of Dental College. Seoul National University. The purpose of this study was compare with normal American blood findings and Korean dental patient's blood findings. The results were as follows: 1. The average values of clinical blood findings dental patients were decreased to the normal values of normal American. 2. White blood cells were not increased in dental patients. 3. The number of lymphocytes was more increased to normal American's blood value.

• The Journal of Korean Medicine
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• v.28 no.2 s.70
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• pp.126-136
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• 2007

Objectives : In the present study, the effect of Kuwonsimsin-hwan (KSS) was tested in methotrexate (MTX)-induced immunosuppressed SD rats. Methods : Methotrexate was fed to white rats once a day for 4 days. After the immune responses of the rats deteriorated, dried extracts of Kuwonsimsin-hwan mixed in water was fed to the rats once a day for 14 days. We then measured the number of lymphocytes in peripheral blood and the percentage of B-cells, T-cells, CD3+CD4+T-cells, CD3+CD8+ T-cells and IL-2 productivity sampled from spleen and peripheral region. Results : (1) The number of lymphocytes and the percentage of T-cells and CD3+CD4+ T-cellsin peripheral blood increased significantly in the KSS group as compared with the control group. (2) The percentage of B-cells, CD3+CD8+ T-cells, and CD4+/CD8+ T-cells in peripheral blood were not different statistically. (3) The percentage of T-cells in spleen and IL-2 productivity of spleen cells increased significantly in the KSS group as compared with the control group. (4) The percentage of CD3+CD4+ T-cells in spleen increased in KSS the group as compared with the control group but without statistical significance. (5) The percentage of B-cells, CD3+CD8+ T-cells, and CD4+/CD8+ T-cells in spleen were not different statistically. Conclusion : It is concluded that Kuwonsimsin-hwan has immunostimulating effect on MTX-induced immunosuppressed SD rats.