• 한국축산식품학회지
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• 제39권6호
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• pp.980-987
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• 2019

This study was conducted to investigate the hypotensive effect of egg white protein (EWP) hydrolysate (EWH) in spontaneously hypertensive rats (SHRs). The hydrolysis of EWP was effectively performed with a combination of 0.5% bromelain and 1% papain at 50℃ for 60 min. The resulting hydrolysate did not elicit an allergic reaction as confirmed by human mast cell activation test. The systolic and diastolic blood pressures of the SHRs fed the EWH diet were observed to be significantly or numerically lower than those of the other groups during the experimental period of 28 d. EWH treatment significantly (p<0.05) upregulated the nitric oxide levels in hCMEC/D3 cells and the plasma of the SHRs compared to those in the control. Moreover, EWH ingestion significantly (p<0.01) reduced the plasma angiotensin II level of the SHRs compared with that in the control. In conclusion, beyond its basic nutritional value, EWH prevents and manages hypertension, and thus can be an invaluable resource for functional food development.

• Animal cells and systems
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• 제5권1호
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• pp.45-50
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• 2001

Rana ovarian follicles consist of oocyte, vitelline envelope, granulosa cells, and theca/epithelial layer. Using scanning electron microscopy, the surface structure of each follicular component was investigated. Changes in oocyte surface during oocyte maturation were also examined. Theca/epithelial layer was almost transparent and some blood vessels and granulosa cells were observed underneath in intact follicle. The number of granulosa cells was estimated to be 6700-7200 per oocyte. The granulosa cells partially overlapped each other and their microvilli penetrated the vitelline membrane via holes present in the vitelline envelope and seemed to be linked to oocyte microvilli. After removal of the vitelline envelope by microforcep, oocyte microvilli were observed on the surface of the devitellined oocyte. The oocyte microvilli formed partial clusters on the surface of white spot area which appears iust before germinal vesicle breakdown (GVBD), whereas they were evenly distributed in other areas. The microvilli became shorter and less dense with oocyte maturation. The lengths of oocyte microvilli in the immature and mature oocyte were 1.5 $\mu$m and 0.6 $\mu$m, respectively. The present study suggests a fundamental structural change occurring on the oocyte surface during maturation.

• 한국임상약학회지
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• 제21권2호
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• pp.145-155
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• 2011

The objective of this study was to determine whether any pretreatment parameters were associated with pharmacological effect or toxicity parameters after vincristine administration and to describe a mathematical model, which explains the interpatient pharmacodynamic variability. The relationship between patient characteristics and vincristine dose and hematological toxicity were evaluated. 68 pediatric and adolescence patients and 107 adults with acute lymphoblastic leukemia were treated with vincristine $1.5mg/m^2/day$ IV and other anticancer drugs as scheduled. Complete blood counts and other blood test results were obtained. The input variables were age, gender, weight, lean body weight (LBW), height, body surface area, vincristine dose and total vincristine dose. The outcome measures were nadir values (white blood cells, absolute neutrophil counts, hemoglobin, and platelets); the absolute decrease, relative decrease, and survival fraction of blood cells. Polynomial regression analysis was carried out to determine the other significant covariates. The variability of $WBC_{nadir}$ was modeled with good precision and accuracy with a two-covariate model. This model should be validated and improved on with further clinical data. We believe that such pharmacodynamic modeling should be explored further to determine its performance and clinical relevance compared with modeling using pharmacokinetic parameter.

• 한국유기농업학회지
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• 제20권4호
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• pp.577-587
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• 2012

본 실험은 허브부식토 및 비타민 C 공급에 따른 송아지의 면역력, 증체 및 건강에 미치는 영향을 조사하기 위하여 대조구, 허브부식토제제 첨가구(T1), 비타민C제제 첨가구(T2), 비타민 C제제와 허브부식토제제의 50 : 50 혼합 첨가구(T3)로 나누고 시험축은 각 시험구당 5두씩 임의 배치하여 수행하였다. 조사항목으로 혈중 대사물질, 사료섭취량, 체중 및 설사빈도를 측정하였다. 혈액성상으로 WBC, NE, LY 및 RBC은 처리간 유의한 차이를 나타내지 않았으나 증체율 및 사료효율은 허브 부식토를 급여한 T1구에서 다른 처리구와 비교하여 유의하게 가장 높았으며, 설사빈도는 허브 부식토+비타민 C 급여한 T3구에서 낮았지만 유의한 차이는 없었다. 따라서 본 연구에서의 Holstein 송아지에게 부식토 급여는 송아지의 면역성 향상에는 분명한 효과를 보이지 못하였으나 증체율과 사료효율에 있어 긍정적인 효과가 있는 것으로 판단되었다.

• Journal of Periodontal and Implant Science
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• 제28권1호
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• pp.187-191
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• 1998

Aplastic anemia is a disease characterized by general lack of bone marrow activity; It may affect not only the red blood cells but also the white blood cells and platelets, resulting in pancytopenia. Spontaneous gingival hemorrhage is present in some cases and it is related to the blood platelet deficiency. This case report presents the periodontal treatment of a patient with aplastic anemia. A 43-year-old female was referred for continuous gingival bleeding after periodontal treatment. Periodontal findings revealed generalized gingival imflammation, oozing of blood from gingival crevice, and it was diagnosed as adult periodontitis. Root planing and extraction of the upper left third molar with poor prognosis were put into operation after elevation of the platelet count with platelet transfusion. The extraction socket was sutured with 3-0 silk. Bleeding continued even after digital compression at the upper right second premolar, second molar, and left canine areas, which presented severe inflammation. Although platelets were transfused repeatedly, platelet count did not stay elevated since survival rate of the transfused platelets were low due to alloimmunization. Thrombin gauze packing was not effective. Bleeding ceased 3 days after treatment with transfusion of donor platelets. 20 days after the treatment, the gingiva was generally healthy except upper right second premolar and lateral incisor areas. The result of periodontal treatment was good, but bleeding control after treatment was troublesome. In the periodontal treatment of patient with aplastic anemia, elevation of the platelet count with platelet transfusion seems to be the best method for hemorrhage control.

• Journal of Radiation Protection and Research
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• 제42권3호
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• pp.146-153
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• 2017

Background: Acute radiation syndrome (ARS) primarily refers to damage to the hematopoietic system, myeloid system, and gastrointestinal (GI) system caused by radiation exposure. Such damage progresses to become life-threatening. In particular, as the syndrome develops very rapidly-within several hours from radiation exposure-prompt and accurate diagnosis and treatment are needed, as is further research into appropriate diagnostic and treatment modalities. Materials and Methods: Minipigs, which display human-like properties, underwent whole-body irradiation at 2 or 4 Gy (doses causing hematopoietic ARS) or at higher doses of 7 or 12 Gy. Changes in the blood cells and clinical symptoms were analyzed and we performed a necropsy when the animals succumbed to ARS. Results and Discussion: The minipig irradiated with 2 Gy showed a decrease in white blood cells, including neutrophils, lymphocytes, and platelets in the early stages. However, the blood cell counts gradually increased and returned to normal values. The minipig irradiated with 4 Gy succumbed due to hematopoietic ARS. In contrast, the minipigs irradiated with 7 or 12 Gy exhibited clinical symptoms of combined GI damage and hematopoietic syndrome. Moreover, a characteristic pattern of platelet changes was observed in the 7 and 12 Gy irradiated minipigs. Conclusion: The changes in the platelet count caused by radiation exposure observed in minipigs, which are hematologically and pathohistologically similar to humans, suggest that they can be used as a novel diagnostic criterion.

• Journal of Ginseng Research
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• 제15권1호
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• pp.13-20
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• 1991

Ginseng components were examined for their ability to modify immunotoxicity of cyclophosphamide. Ginseng polysaccharide fraction (FO, 300 mg/kg) inhibited decreases in the ratio of spleen to body weight, white blood cells (WBC) count and the number of plaque forming cells (PFC) induced by cyclophosphamide (50 mg/kg, i.p.), and increased these variables in normal mice. Ginseng saponin fraction (50 mg/kg) showed to increase hemoglobin content as well as the number of PFC/spleen in normal mice. on the other hand, Panaxytriol (20 mg/kg) Prevented decrease in mc count by cyclophos phamide. Neither saponin fraction nor panaxytriol had any significant effect on the number of PFC and antibody titers in cyclophosphamide-treated mice. These results suggest that ginseng polysaccharine fraction may reduce the immunotoxicity of cyclophosphamide and may be effective in stimulating immune function in normal mice.

• 대한기계학회논문집B
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• 제36권4호
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• pp.391-395
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• 2012

본 논문에서는 서로 다른 밀도의 유체 내 바이오 물질이 받는 중력과 부력 차를 이용한 연속적 세포 분리기를 제안하였다. 종래의 크기별 세포분리는 서로 다른 크기의 동일한 밀도를 가지는 세포를 분리하는데 한계가 있다. 반면, 본 논문에서 제안하는 세포 분리기는 미소유로 상하부에 밀도가 다른 다층 유체층 내에서 세포가 받는 중력과 부력 차이로 크기는 다르지만 동일한 밀도를 가지는 세포를 효율적으로 분리할 수 있다. 밀도가 다른 유체층(PBS, 밀도=1.0g/ml, Ficoll, 밀도=1.1g/ml) 내에서 전혈로부터 백혈구(직경=$6-10{\mu}m$, 밀도=1.06~1.1g/ml), 적혈구(직경=$4-6{\mu}m$, 밀도=1.09~1.2g/ml)를 밀도에 따라 분리한 효율이 각각 $90.9{\pm}9.1%$와 $86.4{\pm}1.99%$로 측정되었다 따라서, 본 세포 분리기는 크기 편차가 있는 동일 밀도의 세포를 크기에 둔감하고 밀도에만 민감한 분리가 가능하다.

• 디지털융복합연구
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• 제14권6호
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• pp.317-323
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• 2016

본 연구의 목적은 백삼, 오미자, 맥문동, 길경, 감초 등으로 구성된 백삼복합물이 난알부민으로 유도된 천식 마우스에서의 천식개선에 대한 융복합 연구를 통한 효능을 확인하는 것입니다. 난알부민으로 천식을 유발한 마우스에 백삼복합물을 100 mg/kg과 300 mg/kg을 경구 투여한 후 혈청 내 Th2 사이토카인 IL-4, IL-5, IL-13을 루미넥스를 이용하여 측정하였다. 또한, 혈청과 혈액 내 난알부민 특이-IgE 수치와 호산구, BALF 내 총 세포수는 자동혈구계산기 및 현미경을 통해 측정하였다. 그 결과, 혈청 내 IL-4, IL-5, IL-13 및 난알부민 특이-IgE 수치가 대조군에 비해 각각 감소하였다. 또한, 혈액 내 호산구 및 BALF 내 총 세포수 역시 감소를 보였다. 이러한 결과는 백삼복합물이 Th2 사이토카인과 천식 요인에 대해 감소가 나타나 효과적인 항천식 치료 약물로서 사용될 수 있음을 시사하는 바이다.

• 한국자연치유학회지
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• 제8권1호
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• pp.21-28
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• 2019

목적: 본 연구는 한국인 대상자에게 12종의 Lactobacillus균주와 4종의 Yeast 혼합 발효용액을 8주간 섭취한 대상자들의 일부 혈액성상에 미치는 영향을 연구하는 것이 목적이었다. 방법: 대상자들의 혈액을 채취하여 임상병리학적 혈액 성분 검사를 실시하였다. 결과: 백혈구 수의 조사에서는. Zen발효액을 섭취한 실험군의 사전 검사치 평균은 5.73×10³ cells/µl, 사후의 평균은 5.37×10³ cells/µl로 감소하였으나 차이 값은 유의성은 없었다. 대조군은 모두 유의성이 없었다. Hemoglobin 함량의 조사에서는 Zen-실험군의 섭취전의 평균 수치는 13.58 g/dl이었고, 섭취 후에는 14.77 g/dl이었으며, 유의성이 있게 증가했다. Albumin 함량 조사에서는 Zen-실험군의 섭취전의 평균 수치는 4.33 g/dl이었고, 섭취 후에는 4.36 g/dl이었으나, 유의성은 없이 증가했다. Triglyceride 함량을 조사에서는 Zen-실험군의 사전 측정치 평균은 109.8 mg/dl, 사후의 측정치 평균은 99.93 mg/dl로 9.87 mg/dl로 감소하였으나 유의성이 없었다. LDL 함량 조사에서는 Zen-실험군의 사전 측정치 평균은 109 mg/dl, 사후의 측정치 평균은 97.87 mg/dl로 나타났으며, 차이 값이 11.13 mg/dl로 유의성이 있게 감소하였다. HDL 함량조사에서는 Zen-실험군은 사전 검사치 평균이 51.4 mg/dl, 사후는 56.87 mg/dl로 나타나 차이 값이 5.47 mg/dl이 증가하여 유의한 차이를 보이었다. 결론: Zen발효액을 섭취한 후에는 실험군의 평균치는 백혈구, albumin, triglyceride는 유의한 차이는 없었고, hemoglobin, LDL 그리고 HDL은 유의한 차이를 보이었다. 인체에서 Zen의 섭취가 혈액 성분의 일부 신진대사에는 영향이 있었다고 판단된다.