• Asian-Australasian Journal of Animal Sciences
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• 제14권11호
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• pp.1542-1548
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• 2001

Wheat straw was treated on laboratory scale with 4% urea at a moisture level of 50% along with different amount of HC1 to fix various levels of ammonia (30, 40, 50 and 60%) and stored for 4 weeks. Result, revealed a significant (p<0.01) increase in CP content of the samples where HC1 was added. The CP content of the straw was only 7.8%, which increased to 14.1, 16.0, 15.0 and 15.2% with the addition of acid. Similarly the concentrations of NDF, ADF and hemicellulose was significantly different due to HC1 addition. The level of HC1 recommended was to trap 30% ammonia as there was not significant difference in CP content of straw due to addition of 4 levels of acids. Results of in vivo experiment conducted on nine buffaloes divided randomly into three groups of three animals in each revealed no significant difference in the intake of DM, OM, NDF, ADF, cellulose and hemicellulose in group I (ammoniated straw), group II (HC1 treated ammoniated straw) and group III (HC1 treated ammoniated straw + 1 kg barley grain), but the intake of CP was significantly (p<0.01) more in group III as compared to other 2 groups. The digestibility of DM, OM and CP was significantly (p<0.01) more in groups where HCI treated straw was fed as compared to only ammoniated straw fed group, whereas there was no significant difference in the digestibility of NDF, ADF and cellulose in 3 groups. Intake was significantly higher of nitrogen (p<0.05), calcium (p<0.01) and phosphorus (p<0.01) in group III as compared to other two groups. Animals in all the 3 groups showed positive nitrogen, calcium and phosphorus balance, though the balances of all the 3 nutrients were significantly higher in group III as compared to other 2 groups. Rumen fermentation study conducted in 3 rumen fistulated buffaloes in $3{\times}3$ latin square design offering the same 3 diets as in group I to III revealed that rumen pH was alike statistically in 3 groups and at various time intervals. The mean ammonia-N concentration was significantly (p<0.01) more in group II and III as compared to group I. The mean TVFA concentration (mM/100 ml SRL) were 6.46, 7.84 and 8.47 in 3 groups respectively and different statistically (p<0.01). Results revealed no significant difference in the activities of carboxy methyl cellulase, urease or protease at both the time of sampling (0 h and 4 h) in all the 3 groups of animals.

• 한국작물학회지
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• 제29권3호
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• pp.267-274
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• 1984

질소의 추비가 소맥엽신의 기공개도에 어떠한 영향을 미치는가를 검토하기 위하여 소맥 조광 품종을 공시하여 질소추비와 무추비구로 처리하여 침윤법으로 최고분얼기, 절간신장기, 수잉기, 개화기, 등숙중기에 기공개도를 측정하였던바 그 결과는 다음과 같다. 1. 소맥엽신의 기공개도는 생육이 진전됨에 따라 증가하여 개화기경에 최대에 달하였으며 그 이후는 저하하였다. 2. 1 일중 기공개도가 최대로 되는 시각은 각시기 모두 정오경이었으며 오후 6시경에는 상당히 폐공 되었으나 이는 생육단계에 따라 다소 상이하였다. 3. 질소의 추비는 엽신의 기공개도를 증가시켰는데 그 정도는 생육이 왕성한 수잉기와 개화기에 특히 큰 경향이었다. 4. 상위엽은 하위엽에 비하여 모든 생육단계에서 큰 기공개도를 나타내었으며, 질소추비에 의한 기공개도의 차이도 상위엽이 하위엽에 비하여 크게 나타나는 경향이었다. 5. 엽신의 질소함량과 1 일중 기공개도의 최대치간에는 고도의 정상관(r=0.66＊＊) 관계가 있었는데, 상위엽과 하위엽 모두 같은 경향이었다. 6. 각 엽기의 엽위간 기공개도의 차는 완전 전개한 최상위엽이 최대치를 나타내었고 전개중인 미성숙엽이나 엽의 노화가 진전된 하위엽일수록 기공개도는 작았다. 7. 각 엽신의 함수중, 근중 및 근의 활력은 질소의 추비에 의하여 증가되었는데 이는 기공개도를 증대시친 한 요인으로 작용한 것으로 추찰되었다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 1999년도 추계 학술대회지
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• pp.102-144
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• 1999

The major problems of food crop cultivation in Korea are low yield of most crops except rice. inefficient cultivation techniques for aged farmers. and low international competibility. Therefore, development of cultivation techniques of food crops should aim the yield. quality improvement, labor reduction and production cost. The primary issue for increasing the yield of soybean, barely and wheat is to reduce the yield gap between the farmer's yield and recommended ones of experiment station. More advanced cultivation techniques needs to be developed. and/or the conventional breeding methods to be reconsidered. The newly developed labor-saving mechanized technique needs to reduce labor hours , and the cost of agricultural implements and machineries. In other words the labor-saving mechanized technique should be developed based on the improvement of total farming system as well as systemic fundamental innovation of cultural methods. The efficiency of solar energy use in food production of Korea in 1997 is as low as $0.52{\%}$ so there is much room to increase yield. It is recommendable that the concept of food Production should be changed to energy Producing efficiency Per unit area basis from volume and weight of food materials. Moreover, introduction of resonable cropping system is needed to increase yield of main crops, farmer's income, solar energy use efficiency, and decrease of land service expenditure. Current cropping system emphasized on economic crops. especially in vegetables , is not desirable for resonable use of arable land. stability of agricultural management and staple food crop self-sufficiency ratio. It is desirable to increase food crops . that are energy of carbohydrate and protein rich and land dependent crops. in cropping system. And the agronomist should develop the cultural methods to replace food crops for food self-sufficiency and stable farming management instead of economic crops in current cropping system. Low-input and environmentally-sound crop cultivation techniques, especially nitrogen-reducing culture technique which is directly related to food crop quality, also needs to be developed urgently. The extended cultivation of corn in upland and barely and wheat in lowland as a feed stuffs is recommended to prevent further decrease of food self-sufficiency ratio, which is mainly caused by the high reliance on imported feed grain. It is also considered that the calculation and presentation methods of standard agricultural income needs to be improved. The current calculation method uses unit land area of 10a regardless of crop kinds , characteristics of agricultural management and cultivation scale. So, it is apt to lead misunderstanding of farm income value. Therefore. it should show an income of average farmers for certain number of years. Research and developing system for food producing is not desirable because they are conducted currently individual crop and mono-culture basis. But actual agricultural income is usually earned by cropping system including upland and lowland. For example. the barley and wheat is usually cultivated in double cropping system. The cooperation among research institutes such as university agribusiness. government and farmers is indispensible. The public information and education on importance and consumption habit of food crops is necessary in Korean society to increase food self-sufficiency through nationwide cooperation.

• 한국식품과학회지
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• 제34권2호
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• pp.232-237
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• 2002

흑미가루 첨가비율에 따르는 식빵의 품질 특성을 조사한 결과 흑미가루의 첨가 비율이 증가함에 따라 빵의 부피는 감소하였다. 실험구간 내에서, 색도의 경우 명도와 황색도는 흑미가루의 증가에 따라 유의적으로 감소하고 적색도는 증가하여(p<0.05) 흑미가루 첨가비율이 식빵의 내부 및 외부 색깔의 변화에 큰 영향을 미침을 알 수 있었다. 조직검사에서 흑미가루의 첨가 비율 증가에 따라 hardness(견고성), gumminess(점착성), chewiness(씹힘성)는 증가하고 springiness(탄력성), cohesineness(응집성)는 감소하였다. 관능검사에서 흑미첨가 비율 증가로 기공, 조직, 외형, 터짐성 등 품질 특성은 낮게 평가되었지만 내부 색상과 향에 대한 기호도는 증가였으며 맛은 $10{\sim}20%$ 사이에서 밀가루 식빵과 차이가 없었다. 전체적인 기호도는 흑미가루를 20% 첨가한 것이 밀가루 식빵과 유의적인 차이를 나타내지 않았다(p<0.05). 그러므로 흑미 특유의 색, 향 및 영양기능성을 고려하였을 때 흑미가루를 20%까지 첨가해도 빵의 품질특성에 큰 영향을 미치지 않을 것으로 생각된다.

• 한국식품영양과학회지
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• 제30권6호
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• pp.1120-1128
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• 2001

한국형 찐빵 품질에 적합한 시중 밀가루 품질과 발효 시간 (0~60분) 및 첨가 재료(베이킹파우더, 쇼트닝)가 찐빵 품질에 미치는 효과를 조사하고자 박력분(LF), 중력분(MF), 강력 분(HF)과. 박력분과 강력분(HLF) 혹은 박력분과 중력분 (HMF)을 각각 동량 혼합한 것을 포함한 5종의 밀가루를 사용하여 밀가루 반죽 물성과 빵 품질을 비교하였다. 사용된 밀가루의 단백질 함량은 8.17.~12.52%, 회분은 0.38 ~ 0.41% 범위에 있으며 찐빵은 오븐에서 굽는 roll bread와는 다르게 1차 발효 과정을 생략한 제조 조건에서 찐빵 품질이 가장 우수하였고, 찐빵 제조에 요구되는 밀가루는 반죽 강도가 강한 강력분에 가까운 밀가루보다 단백질 함량이 9.5 ~ 10.9%, 범위의 반죽 강도가 중간 정도인 시중 밀가루가 찐빵 제조에 적합하였다. 찐빵 제조에 베이킹파우더 1.0~1.5%, 쇼트닝 5 ~ 7% 첨가로 빵 부피, 빵 모양, bread score, 조직감 등이 향상되었다. 전반적인 기호도가 가장 높았던 찐빵은 강력분과 박력분을 동량 혼합한 혼합분(단백질 10.53%)으로 만든 것으로 빵 껍질이 매끄럽고, 광택이 있고 희었으며, 촉감은 촉촉하고 부드러웠다. 시중 밀가루 품질과 빵 품질과의 상관성은 ro11 bread와 찐빵에서 다르게 나타났는데 roll bread의 빵 부피나 total bread score는 단백질 함량과 farinograph 반죽 물성이 모두 관련이 있었으나 찐빵의 경우 단백질 함량은 빵 부피에 영향을 미치지 않았고 새심 bread score는 단백질 보다 반죽 안정도와 상관성이 더 높았으며 빵 표면 특성은 반죽 형성 시간과 호화 점도에 의해 더 큰 영향을 받는 것으로 나타났다.

• 한국식품과학회지
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• 제37권5호
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• pp.736-740
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• 2005

Yeast로 발효시킨 빵을 섭취한 후 호흡기 증상이 나타났다는 보고에서 yeast가 생성한 ${\alpha}-amylase$가 알레르기의 원인(22,23)임이 밝혀졌으므로 본 연구에서는 yeast 대체 첨가제로 sodium bicarbonate를 선택하였으며, 첨가제의 농도가 다른 12종류의 반죽을 제조하였다. Meissle 발효관에 의한 $CO_2$ 발생량 비교 실험 결과, sodium bicarbonate 첨가를 많이 할수록 $CO_2$ 발생량이 증가함을 알 수 있었다. 그러나 hypo-allergenic breads(HAB) 제조 시 2.0% 이상 첨가한 HAB 7 이상에서는 sodium bicarbonate의 쓴맛이 강하게 나타나 관능 특성 평가 결과 기호도가 떨어지는 것으로 드러났다. 체적과 무게에 대한 비로 빵의 품질을 조사한 결과, 체적의 경우 hypo-allergenic bread (HAB) 11, 12가 가장 높은 값을 보였다. HAB 12, 9, 11이 가장 많이 부푼 빵이 되었으며 HAB 1은 빵의 부피가 현저하게 적어 제빵용 조합으로는 부적합하였다. 관능검사에서 색, 향, 맛 항목에서 유의적인 차이를 보였다. 색은 HAB 7이, 향과 맛 에서는 HAB 5가 가장 우수한 결과를 나타내었으며 기공과 촉감항목은 유의적인 차이를 나타내지 않았다. 마지막으로 전체적인 기호도 평가 시 매우 유의적인 차이를 보이며 HAB 5가 우수하게 나타났다. 결과적으로 hypo-allergenic bread(HAB) 시작품은 b.p. protease로 gluten을 분해하여 항원성을 저감화 시킨 국내산 밀 반죽에 sodium bicarbonate 1.5%, sucrose 1.0%, NaCl 1.0%, citric acid 1.0%를 첨가하여 제작되었다.

• 농업과학연구
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• 제45권3호
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• pp.355-363
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• 2018

This study was conducted to investigate suitable rice cultivars for various rice based cropping systems in a paddy field in the Yeongnam plain area. Thirteen rice cultivars (5 early, 3 medium and 5 mid-late maturing cultivars) and three transplanting dates (June 5, June 25 and July 5) were evaluated in this study. The mid-late and early maturing cultivars for the July 5 and June 25 transplanting headed before August 30 which is the safe heading date in the Yeongnam plain area, ranging from Aug. 26 - 28 and Aug. 18 - 23, respectively. The safe harvest time of rice for double and triple cropping systems should be before the middle of October for the cultivation of the succeeding winter crops. The rice yield was the highest for the June 5 transplanting regardless of the rice cultivars, and it gradually decreased as the transplanting date was delayed from June 5 to July 5 due to a decrease in the spikelet numbers per panicle number and in the ripened grain rate. In contrast, the other yield parameters that include the panicle number per $m^2$, the 1,000-brown rice weight, and the ripened grain ratio were not significantly affected. The result indicates that based on the milled rice, heading time and harvest time, a medium maturing cultivar (Haiami and Samdeog) would be applicable to a winter barley/wheat, garlic/onion-summer rice double cropping, while a mid-late cultivar (Saeilmi, Saenuri and Hyunpoom) would be suitable for a spring potato/waxy corn-summer rice double cropping in the Yeongnam plain area. On the other hand, an early maturing cultivar (Unkwang, Jokwang and Haedamssal) would be preferable for a triple cropping because of the short growth period of rice.

• 한국균학회지
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• 제47권4호
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• pp.347-357
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• 2019

본 연구에서는 urushiol 제거에 적합한 장수버섯 종균용 원료에 대하여 평가하였다. 세 가지 곡물조, 현미, 통밀에서의 장수버섯 균사체 증식속도는 각각 4.92±0.05, 2.20±0.03, 1.93±0.03 mm/day 이었다. 장수버섯이 배양된 곡물종균의 laccase의 활성은 각각 0.86±0.02, 0.04±0.01, 0.01±0.00 U/mL 이었다. 증식속도와 효소활성 측면에서 장수버섯 종균용 곡물로는 조를 사용하는 것이 적합하였다. 조에 배양된 장수버섯 종균을 옻나무에 배양하였더니 3일차에 urushiol이 최대 86.6% 감소하였고 7일차에 98.5%까지 감소하였다. Urushiol 제거를 위한 적정 배양기간은 3일이었으며 이때 flavonoid 잔존량은 68%, phenolic 성분의 잔존량은 42% 이었다. 액체종균을 사용하여 배양한 것과 비교했을 때 urushiol을 제거하기 위한 증식기간이 10일에서 3일로 단축되었다.

• The Plant Pathology Journal
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• 제34권2호
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• pp.104-112
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• 2018

Accurate and rapid detection of bacterial plant pathogen is the first step toward disease management and prevention of pathogen spread. Bacterial plant pathogens Clavibacter michiganensis subsp. nebraskensis (Cmn), Pantoea stewartii subsp. stewartii (Pss), and Rathayibacter tritici (Rt) cause Goss's bacterial wilt and blight of maize, Stewart's wilt of maize and spike blight of wheat and barley, respectively. The bacterial diseases are not globally distributed and not present in Korea. This study adopted comparative genomics approach and aimed to develop specific primer pairs to detect these three bacterial pathogens. Genome comparison among target pathogens and their closely related bacterial species generated 15-20 candidate primer pairs per bacterial pathogen. The primer pairs were assessed by a conventional PCR for specificity against 33 species of Clavibacter, Pantoea, Rathayibacter, Pectobacterium, Curtobacterium. The investigation for specificity and sensitivity of the primer pairs allowed final selection of one or two primer pairs per bacterial pathogens. In our assay condition, a detection limit of Pss and Cmn was $2pg/{\mu}l$ of genomic DNA per PCR reaction, while the detection limit for Rt primers was higher. The selected primers could also detect bacterial cells up to $8.8{\times}10^3cfu$ to $7.84{\times}10^4cfu$ per gram of grain seeds artificially infected with corresponding bacterial pathogens. The primer pairs and PCR assay developed in this study provide an accurate and rapid detection method for three bacterial pathogens of grains, which can be used to investigate bacteria contamination in grain seeds and to ultimately prevent pathogen dissemination over countries.

• Asian-Australasian Journal of Animal Sciences
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• 제27권7호
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• pp.946-950
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• 2014

Bacillus licheniformis was grown in minimal nutrient medium containing 1% (w/v) of distillers dried grain with soluble (DDGS), palm kernel meal (PKM), wheat bran (WB) or copra meal (CM), and the enzyme activity of endoglucanase, ${\beta}$-glucosidase, xylanase and reducing sugars was measured to investigate a possibility of using cost-effective agricultural residues in producing cellulolytic and hemicellulolytic enzymes. The CM gave the highest endoglucanase activity of 0.68 units/mL among added substrates at 48 h. CM yielded the highest titres of 0.58 units/ml of ${\beta}$-glucosidase, compared to 0.33, 0.23, and 0.16 units/mL by PKM, WB, and DDGS, respectively, at 72 h. Xylanase production was the highest (0.34 units/mL) when CM was added. The supernatant from fermentation of CM had the highest reducing sugars than other additional substrates at all intervals (0.10, 0.12, 0.10, and 0.11 mg/mL respectively). It is concluded that Bacillus licheniformis is capable of producing multiple cellulo- and hemicellololytic enzymes for bioethanol production using cost-effective agricultural residues, especially CM, as a sole nutrient source.