The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Effect of Perenniporia fraxinea on Eliminating Urushiol from Rhus verniciflua Stokes Stem Bark

아까시재목버섯이 옻나무 껍질의 urushiol 제거에 미치는 영향

  • Lee, Ji-Hyun (Department of Agrofood Resources, National Academy of Agricultural Science, RDA) ;
  • Jeong, Seok-Tae (Department of Agrofood Resources, National Academy of Agricultural Science, RDA) ;
  • Kang, Ji-Eun (Department of Agrofood Resources, National Academy of Agricultural Science, RDA) ;
  • Choi, Han-Seok (Department of Agriculture & Fisheries processing, Korea National College of Agriculture and Fisheres)
  • 이지현 (농촌진흥청 국립농업과학원 발효가공식품과) ;
  • 정석태 (농촌진흥청 국립농업과학원 발효가공식품과) ;
  • 강지은 (농촌진흥청 국립농업과학원 발효가공식품과) ;
  • 최한석 (한국농수산대학 농수산가공학과)
  • Received : 2019.11.19
  • Accepted : 2019.11.28
  • Published : 2019.12.31
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Abstract

This study evaluated the raw materials for spawning Perenniporia fraxinea, to eliminate urushiol. The growth rates of spawns on grains of millet, brown rice, and wheat were 4.92±0.05, 2.20±0.03, and 1.93±0.03 mm/day, respectively, and the laccase activity was 0.86±0.02, 0.04±0.01, and 0.01±0.00 U/mL, respectively. These observations revealed millet as the most appropriate grain for spawn production in terms of growth rate and enzyme activity. Inoculation of lacquer tree (Rhus verniciflua Stokes) stem bark with millet spawns of P. fraxinea resulted in a reduction of its urushiol contents, up to 86.6% on the third day and up to 98.5% on the seventh day. The optimal period of cultivation to eliminate urushiol was three days with 68% of residual flavonoids and 42% of phenolic components. When compared to the product cultivated from liquid spawn, the millet spawn reduced the cultivation period from 10 days to 3 days for eliminating urushiol.

본 연구에서는 urushiol 제거에 적합한 장수버섯 종균용 원료에 대하여 평가하였다. 세 가지 곡물조, 현미, 통밀에서의 장수버섯 균사체 증식속도는 각각 4.92±0.05, 2.20±0.03, 1.93±0.03 mm/day 이었다. 장수버섯이 배양된 곡물종균의 laccase의 활성은 각각 0.86±0.02, 0.04±0.01, 0.01±0.00 U/mL 이었다. 증식속도와 효소활성 측면에서 장수버섯 종균용 곡물로는 조를 사용하는 것이 적합하였다. 조에 배양된 장수버섯 종균을 옻나무에 배양하였더니 3일차에 urushiol이 최대 86.6% 감소하였고 7일차에 98.5%까지 감소하였다. Urushiol 제거를 위한 적정 배양기간은 3일이었으며 이때 flavonoid 잔존량은 68%, phenolic 성분의 잔존량은 42% 이었다. 액체종균을 사용하여 배양한 것과 비교했을 때 urushiol을 제거하기 위한 증식기간이 10일에서 3일로 단축되었다.

Keywords

References

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