• Journal of Life Science
• /
• v.10 no.4
• /
• pp.422-430
• /
• 2000

In order to search for the effects of Shine-Dalgarno (SD) sequence and nucleotide sequence of spacer region (SD-ATG) on bGH expression, oligonucleotides containing random SD sequences and a spacer region were chemically synthesized. The distance between SD region and initiation codon (ATG) was fixed to 9 nucleotides in length. The expression vectors have been constructed using pT7-1 vector containing a T7 promoter. Positive clones were screened with colony hybridization and named pT7A or pT7B plasmid series. The selected clones were confirmed by DNA sequencing and finally, 19 clones having various SD combinations were obtained. When bovine growth hormone was induced by IPTG in E. coli BL21(DE3), all cells harboring these plasmids produced a detectable level of bGH in western blot analysis. However, various SD sequences did not affect on bGH expression, indicating that the sequences of SD and the spacer region did not sufficiently destabilize mRNA secondary structure of bGH gene. Therefore, these results indicate that the disruption of mRNA secondary structure might be a major factor for regulating bGH expression in the translational initiation process.

• The Journal of Internal Korean Medicine
• /
• v.18 no.1
• /
• pp.86-96
• /
• 1997

We examined clinical and reseach data about abdominal palpation conducted in japanese oriental medicine from the early stages of its medical history to the latest research, so as to introduce it into korean oriental medicine. That is to say, we studied clinical significance and suitable oriental medicine prescription about following : sweat and moistness of abdominal skin ; temperature of abdominal skin ; palpation and visible peristaltic movement ; meteorism ; tonus of the whole rectus abdominis muscle ; local tonus of parts rectus abdominis muscle ; palpitation of abdominal aorta ; resistance tender on pressure in epigastric region, subcostal region, umbilical region, lower abdomen, para-umbilical region, cecal region, sigmoid region and inguinal region ; sound of fluctuating liquid in the epigastric region. The result was that abdominal palpation was an immediately applicable method of clinical diagnosis in oriental medicine, and is being proven by western medicine methodology today. Therefore we suggest that korean oriental medicine would advance greatly if abdominal palpation were applied.

• Journal of architectural history
• /
• v.31 no.4
• /
• pp.71-86
• /
• 2022

This study examines the process of the introduction of stupa, which has originated in India, to the Xinjiang Uyghur region and the features of the stupas in the Xinjiang Uyghur region in detail. This study examines the layout of the buildings in temple compounds and the types, structural elements, and construction methods of the stupas in the Xinjiang Uyghur region in particular by looking into the content of the Da Tang Xi Yu Ji and remaining stupas, which provide examples of stupas at the time. This study finds that due to the characteristics of dry deserts, stupas in Xinjiang Uyghur region, where assimilation between Eastern and Western cultures is seen, were mostly made by pressing clay into a mold and had no interior spaces. Also, construction materials and techniques had been developed and improved in a way that enabled stupas to combat the challenging desert conditions. However, the stupas in this region differed significantly from the wooden tower-like stupas discovered in central China(zhongyuan 中原). The shape of the dome of most stupas in Xinjiang Uyghur region was chosen under the influence of the Gandharan style. Some of the stupas in the region have taken the general forms of the wooden stupas and the others have taken many forms, from cylindrical drums to towers. Also, there have been forests of stupas and stupas similar in form to chaityas and stupas of Vajrayana. Such different forms were transformed and modified through regional history and it was related to the peoples and cultures that produced and used stupas. Stupas evolved into distinct forms in Xinjiang Uyghur region in this way.

• The Journal of Engineering Geology
• /
• v.32 no.2
• /
• pp.257-270
• /
• 2022

This study evaluated the hydraulic factors contributing to the decreasing groundwater levels across Jeju island. Time-series data for groundwater level, precipitation, and groundwater usage and information on land use were acquired, and the correlations among them were analyzed to evaluate the causes of the decreasing groundwater. The effects of precipitation and groundwater usage on the fluctuations of groundwater level were quantified using response surface analysis and sensitivity analysis, and methods for groundwater quantity management by region were proposed. The results showed that the rate of groundwater decrease in the western region was larger than that in the eastern region. For the eastern region, the influence of precipitation was large and the rate of decrease in the groundwater level was relatively small. The geological formation of this part of the island and continuous seawater intrusion suggest that although the absolute amount of groundwater extracted for use was large, the decrease in the groundwater level was not seen to be great due to an increase in pressure by seawater intrusion. Overall, precipitation and groundwater usage had the greatest effect on the amount of groundwater in the western region, and thus their data would be most useful for informing groundwater management, whereas other factors (e.g., sea level and the location of the freshwater-seawater transition zone) must be considered when understanding Jeju's eastern region. As the characteristics of groundwater level fluctuations in the eastern and western regions are distinct, an optimal management plan for each region should be proposed to ensure the efficient management of groundwater quantity.

• Journal of Environmental Science International
• /
• v.19 no.12
• /
• pp.1343-1354
• /
• 2010

The purpose of the study was to analyze the persistence of HCH in atmosphere, soil, sediment and water of the western and southern regions of Korea. The samples from the western region were collected from Anmyeon Island, and the samples from the southern region were collected from Kimhae and Busan. The concentration of HCH isomers in atmosphere showed the pattern of $\alpha$-HCH>$\gamma$-HCH>$\beta$-HCH. The regions with high HCH concentration in the atmosphere are the regions that have been highly exposed to HCH used in the past, and the areas that have been influenced by the long range transport. The HCH that persists in the soil, water and sediment evaporates into the atmosphere, showing the characteristics of Air-Surface exchange. When the regional concentration distributions are compared, the concentration of HCH was higher in the atmosphere of a plain and the cities near the plain, than the urban areas. In this study, the ratio of $\alpha/\beta$-HCH was used as an indicator for estimating the source of Technical HCH and Lindane. According to the result, the contribution of Lindane was high in Kimhae plain and Kimhae urban areas. However, in Busan, the contribution of Technical HCH was higher than Lindane. In case of Anmyeon Island, the western region of Korea had high contribution from Tehcnical HCH. In soil and sediment, $\beta$-HCH was dominant. In water, $\gamma$-HCH was dominant among other isomers. Such results are due to $\gamma$-HCH inLindane. Furthermore, the source of $\gamma$-HCH in urban areas is assumed to be the use of medicine, medical supplies and other living supplies. Based on the results of this study, the management of HCH, a newly list up emerging POPs, should be strengthened by further research on sources, fate, persistency, accumulation and exposures and etc. to the risk assessments.

• Journal of the Korean Society of Marine Environment & Safety
• /
• v.15 no.1
• /
• pp.17-24
• /
• 2009

This study was conducted to find out the effects of relationship between ocean-meteorological factors and snowfall at Incheon, Gunsan and Mokpo when cold and dry continental air mass passed through the West Sea of Korea in winter. Mean snowfall from December to February showed the order of Gunsan (12.7 cm), Mokpo (9.0 cm) and Incheon (7.8 cm). In particular, the snowfall in the three regions showed the regional difference in December and February. It was well consistent with the extension of continental high. Extension of continental high can cause effect on snowfall at the west regions (Inchoen, Gunsan, Mokpo) of the Korean Peninsula. The continental high extended from the southern China to western coastal region of the Korean Peninsula in December, it extended from the northern China to central area of the Korean Peninsula in January. It also extended from the north side of China through Bohai Sea and Yodong Peninsula to central area of the Korean Peninsula in February. Therefore, more snowfall recorded in Incheon is higher in February than December whereas Gunsan and Mokpo is the opposite. The heavy snowfall at the three regions was caused by loss of the heat from the ocean to air when the heat loss was higher than 100 $W/m^2$. The heavy snowfall was also observed when the arrangement of continental high atmospheric pressure and low pressure was high at the west and low at the east, which formed a front in West and when the wind blow from the North or North West at the speed of 4${\sim}$8 m/sec.

• Journal of the Korean association of regional geographers
• /
• v.17 no.2
• /
• pp.150-166
• /
• 2011

This study aims to investigate the differences of synoptic characteristics and frontal structures over East Asia according to the main path types of water vapor flux (WVF) of 850hPa surface in cases of the heavy rainfall in the south coastal region of Korea during the Changma season (June and July), In the cases of type A in which the main path of WVF is running from the South china Sea via the South china to the South Sea of Korea, the North Pacific subtropical anticyclone (NPSA) expands to the South China and strong cyclones appear in the Yellow Sea. In cases of type B and C in those the main paths of WVF are running from the South China Sea via the Western Pacific Ocean near Taiwan to the South Sea and from the Western North Pacific Ocean to the South Sea respectively, tropical cyclones appear frequently near Taiwan and the NPSA shifts northward. In the case of type D in which the main path of WVF appear only near the South Sea, strong cyclones appear near the Yellow Sea. In all cases upper jets are intensified in the northern part of the heavy rainfall region and low-level jets appear near the main paths of WVF. In the view of frontal structure, deep active-type of the Changma front is identified in most cases of all types. In this point the Changma season is different from the Baiu season in Western Japan where many cases of shallow active-type of the Baiu front appear.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
• /
• v.20 no.1
• /
• pp.16-28
• /
• 2015

In order to elucidate the spatiotemporal variations of marine environmental parameters, we collected seawater samples in the south-western region of the East Sea in May, August, and November 2012 and February 2013. The concentrations of dissolved inorganic nutrients (dissolved inorganic nitrogen, phosphorus, and silicate) in surface seawater during the summer season were lower than those during autumn and winter seasons, which the mixed layer is deeper. The low nutrient concentration in spring and summer seasons seems by consumption of dissolved inorganic nutrients by phytoplankon photosynthesis (high chlorophyll a concentration) and the limited supply of dissolved inorganic nutrients from subsurface layer having high nutrients. The low nutrient concentration during spring season seems to be related to the limited supply of dissolved inorganic nutrients from land and subsurface layer because the concentration of chlorophyll a was low. The DIN:DIP ratio was a wide range of average $15.6{\pm}13.6$ in the surface seawater compared to that of average $14.8{\pm}4.2$ in the bottom seawater during sampling periods. The dissolved inorganic nitrogen might act as a limiting factor of the growth of phytoplankton because the DIN:DIP ratio (on average $8.35{\pm}4.67$) was low during the spring season.

• Journal of Ecology and Environment
• /
• v.29 no.6
• /
• pp.573-580
• /
• 2006

A sediment core from the Cheollipo arboretum ($36^{\circ}$ 47' 57'N, $126^{\circ}$ 09' 04') was studied for pollen analysis in order to reconstruct postglacial vegetational change and environmental changes around the central western region of the Korean Peninsula. The record shows four pollen assemblage zones: Zone CHL-I, Quercus stage (ca. 9,300$\sim$6,200 yr BP): zone CHL-II, Quercus-Pinus stage (ca. 6,200$\sim$4,600 yr BP); zone CHL- III, Pinus-Quercus stage (ca. 4,600$\sim$1,160 yr BP): zone CHL-IV and Pinus stage (ca. 1,160 yr BP-present). During the 9,300$\sim$8,500 yr BP, the early Holocene, researchers have guessed a piece of cool-temperate norihern/altimontane mixed coniferous and deciduous broad-leaved forest. Between 8,500$\sim$4,600 yr BP the Quercus dominated the landscape of study area and the established dates of this typical cool-temperate central/montane deciduous broad-leaved forest vegetation might be ca. 6500 yr BP, and then the Pinus developed around the site at ca. 5,700 yr BP. The abrupt increase of Pinus and NAP (non-arboreal pollen) after ca. 1,100 yr BP indicates the vegetation changes due to human activities. From the dynamics of the Chenopodiaceae pollen indicating salt marsh and freshwater pollen flora such as Typha, Trapa, Nymphoides and so forth, we suggest that the tidal flat was altered into freshwater lake around 6,500 yr BP.

• Pediatric Infection and Vaccine
• /
• v.31 no.1
• /
• pp.1-11
• /
• 2024

Despite improvements in vaccine coverage, a resurgence of measles has been reported, especially in the infant and adult populations in recent years. We conducted a systematic review of seroprevalence studies conducted in the Western Pacific Region (WPR) to provide insights into seropositivity trends in different countries. This systematic review aimed to collect data from all available measles seroprevalence studies to characterize the differences in population immunity against measles in different countries. We searched the online databases PubMed and Embase to identify: 1) observational studies that investigated seroprevalence in all age groups, and 2) results reported as antibody levels. The following variables were extracted from different study arms: paper identification (title, first author, publication year), inclusion and exclusion criteria, study site, age of subjects, number of subjects, country/area, population, methods, and seropositivity (%). The search yielded a total of 69 studies included in the review. Among the 1-6-year-old group, seropositivity remained relatively high, at 81-100% in China, 86-94% in Korea, and 77-91% in Australia. In adolescents aged 7-18-years old, seropositivity was relatively constant in China and Australia over time; however, a decreasing trend was noted in Korea in 2011 (66%), 2014 (69%), and 2014 (50%) in this age group. A similar downward trend was observed among Korean adults aged 19-39 years in 2011 (74%), 2019 (71%), and 2019 (64%). Children are likely to be protected by universal vaccination programs in WPR countries and regions. However, susceptible individuals with waned immunity may be present among the adult population.