• 대한임상검사과학회지
• /
• 제36권2호
• /
• pp.137-143
• /
• 2004

Erythrocyte sedimentation rate(ESR) measurements were performed in a total of 191 blood samples from patients below a hematocrit of 35% by both the Test-1(SIRE Analytical Systems, Udine, Italy) and the Westergren method, endorsed as the reference method for ESR by the International Council for Standardization in Hematology(ICSH). The corrected Westergren ESR values were also obtained applying the formula of Fabry (corrected ESR = ESR measured x 15/55-Hct). Linear regression analysis showed a close correlation (r=0.85) between the two methods both before and after ESR correction in total samples. There was no significant correlation difference between two methods in both two groups with hemoglobin $${\geq_-}9g/dl$$ and <9g/dl before and after ESR correction (r=0.873, r=0.827 respectively before correction and r=0.867, r=0.830 respectively after correction). Also, for two groups with hematocrit $${\geq_-}24%$$ and <24%, no significant difference was observed (r=0.859, r=0.792 respectively before correction and r=0.782, r=0.842 respectively after correction). However, for samples with <60mm of Westergren ESR before correction, a better correlation coefficient was obtained than samples with $${\geq_-}60mm$$(r=0.701, r=0.541 respectively). In corrected Westergren ESR, also samples that were obtained with <40mm showed better correlation than samples with $${\geq_-}40mm$$ (r=0.690, r=0.347 respectively). In conclusion, we found a good correlation between Test-1 and Westergren measurements in patients with anemia but an expert group discussion is required to solve discrepancy between two methods in blood samples with very high ESR.

• Journal of Yeungnam Medical Science
• /
• 제13권1호
• /
• pp.110-115
• /
• 1996

적혈구 침강속도는 전통적인 검사의 하나로서 진단에 있어서는 다소 비특이적인 검사이지만 몇몇 질병의 activity를 평가하고 추적검사에 유용한 것으로 평가되고 있다. Westergren법이 표준화된 검사법으로 인정받고 있으나 보고자에 따라 CV값이 0.8-22.9%로 보고되고 있다. 저자들은 143명의 검체를 대상으로 ESR 자동화 기기인$SEDIsystem^{TM}$ 사용하여 ESR을 측정하고 modified Westergren법, Wintrobe법과 비교하여 임상에 적용될 수 있는지를 검토하였다. $SEDIsystem^{TM}$로 측정한 ESR 결과와 modified Westergren법으로 측정한 ESR 결과사이의 상관식 및 상관계수(r)는 y = 0.863x - 1.69 (r=0.830)이었고, $SEDIsystem^{TM}$로 측정한 ESR 결과와 Wintrobe법으로 측정한 ESR 결과사이의 상관식 및 상관계수(r)는 y = 1. 14x - 14.7 (r=0.789)이었고, 검체의 안정성을 평가하기 위해 시간에 따른 ESR의 반복 측정치는 통계적으로 유의한 차이는 없었다. 위의 결과로 볼 때 $SEDIsystem^{TM}$을 이용한 검사는 기존의 modified Westergren, Wintrobe법들과의 상관성이 높았고 4시간까지 안정된 결과를 보였으며 또한 대량의 검체를 신속하게 보고할 수 있어서 임상 혈액검사실에서 이용하기에 적합하다고 생각된다.

• 대한수의학회지
• /
• 제32권3호
• /
• pp.421-427
• /
• 1992

The study was basically carried out to establish the appropriate condition for applying the Westergren method for erythrocyte sedimentation rate(W-ESR) in Throughbred racehorses at recess. To do this, we examined the correlationship among some factors including the kind of anticoagulants, optimal ambient temperature and reading time for W-ESR in healthy racehorses. The difference between the blood samples treated with only 3.8% solution of sodium citrate(SC) and both EDTA and SC as a coagulant, there was not recognized any significant difference(p<0.05) in the levels of W-ESR irrespective of the ambient temperature of $20^{\circ}C$ or $30^{\circ}C$. The best optimal ambient temperature for W-ESR in horses was proved at $30^{\circ}C$ resulting from the tendency of the most reduced dispersion in mean values analyaed from repeatly 4 times to the same blood samles compared with those of $10^{\circ}C$ and $20^{\circ}C$. The optimal reading time was determined as 60 minutes(Y=237.2~4.1X, $r^2=0.998$) and 70 minutes(Y=247.8~4.2X, $r^2=0.999$) under the same temperature of $30^{\circ}C$; the latter showed the better result on the basis of the correlation of packed cell volume(PCV) and ESR values. About 13 healthy racehorses, we compared the real values of W-ESR respectively obtained at 60 minutes and 70 minutes at $30^{\circ}C$ with the anticipated values of PCV by means of the analysis of linear regression equitation. As the result of this, the strong correlation between both of them was confirmed. For practical use of W-ESR in Thoroughbred racehorese, we can recommend the condition of 60 or 70 minutes for the optimal reading time as well as $30^{\circ}C$ for the best ambient temperature.

• 한국가시화정보학회지
• /
• 제19권1호
• /
• pp.57-61
• /
• 2021

In order to determine erythrocyte sedimentation rate (ESR) indicating acute phase inflammation, a Westergren method has been widely used because it is cheap and easy to be implemented. However, the Westergren method requires quite a long time for 1 hour. In this study, a gated recurrent unit (GRU) neural network was used to reduce measurement time of ESR evaluation. The sedimentation sequences of the erythrocytes were acquired by the camera and data processed through image processing were used as an input data into the neural network models. The performance of a proposed models was evaluated based on mean absolute error. The results show that GRU model provides best accurate prediction than others within 30 minutes.

• 대한수의학회지
• /
• 제26권1호
• /
• pp.175-185
• /
• 1986

The measurement of angled erythrocyte sedimentation rate (ESR), as a replacement for perpendicular ESR, for cattle blood was scrutinized since it has been well known that perpendicular ESR in cattle is too slow to be adopted as an effective clinical test. Samples of blood were taken from 186 Korean native cattle over 2 years old. The results obtained in the experiment were summarized as follows. 1. Average values of perpendicular ESR/24hrs in 15 apparently healthy cattle, as measured by Wintrobe, Westergren and capillary tubes, were $5.8{\pm}2.2$, $11.1{\pm}3.7$ and $10.4{\pm}4.5%$ respectively, which were found to be similar to the values of perpendicular ESR/hr of normal blood of human. 2. The ESR was determined in the tubes held at 90, 75, 60, 45, 30 and 15-degree angles, using 3 types of tubes. For the diagnostic purposes, the best results were obtained from the tubes held at 45-degree angle. 3. The angled ESR values increased as the diameters of the tube-bores decreased. 4. The tube length did not affect the angled ESR(%). 5. The angled ESR values increased with the increased environmental temperature during the ESR measurement. 6. The storage temperature at $5^{\circ}C$, $20^{\circ}C$ and $35^{\circ}C$, of the blood for 24 hours did not affect the angled ESR. 7. Samples of blood were treated with 4 kinds of anticoagulants (heparin, $K_2$-EDTA, double oxalate and sodium citrate) and the ESR was determined at 45-degree angle, using capillary hematocrit tubes. The ESR values were higher in the blood samples treated with sodium citrate than in those treated with other anticoagulants. 8. By using the autologous plasma, the PCV was adjusted to be 5, 10, 20, 30, 40 and 50ml/100ml and the ESR was determined in the capillary hematocrit and Wintrobe tubes held at 45 degrees. In both of the methods the ESRs increased as the values of PCV decreased. The regressions of ESR to PCV in both 45-degree-angled capillary and Wintrobe tubes were curvilinear. For the capillary hematocrit tubes the second degree polynomial $Y=61.9779-2.3533x+0.0228x^2$ (r=0.9999) fits the data. And in the case of Wintrobe tubes the second degree polynomial $Y=27.9767-1.1314x-0.0117x^2$ (r=0.9998) fits the data. 9. The 45-degree angled ESR was determined in the blood of 71 healthy Korean native cows using capillary hematocrit tubes. The average PCV was $35.4{\pm}3.6ml/100ml$. The observed ESR/hr averaged $7.2{\pm}2.7%$, while the corrected ESR/hr to a PCV of 36ml/100ml averaged $6.6{\pm}1.3%$. From these results it was concluded that to obtain the best results the ESR/hr of Korean native cattle should be determined at 45-degree angle at room temperature($20^{\circ}C$) using capillary hematocrit tubes.